Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4684 and Pf1N1B4_4685 overlap by 4 nucleotides Pf1N1B4_4685 and Pf1N1B4_4686 overlap by 4 nucleotides
Pf1N1B4_4684 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 5,171,667 to 5,174,465
_4684
Pf1N1B4_4685 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 5,174,462 to 5,176,540
_4685
Pf1N1B4_4686 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 5,176,537 to 5,178,291
_4686
Position (kb) 5174
5175
5176
5177 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5173.720 kb on + strand, within Pf1N1B4_4684 at 5173.720 kb on + strand at 5173.720 kb on + strand, within Pf1N1B4_4684 at 5173.720 kb on + strand, within Pf1N1B4_4684 at 5173.721 kb on - strand, within Pf1N1B4_4684 at 5173.721 kb on - strand, within Pf1N1B4_4684 at 5173.835 kb on + strand, within Pf1N1B4_4684 at 5174.342 kb on - strand at 5174.371 kb on + strand at 5174.947 kb on + strand, within Pf1N1B4_4685 at 5175.111 kb on + strand, within Pf1N1B4_4685 at 5175.117 kb on + strand, within Pf1N1B4_4685 at 5175.499 kb on - strand at 5175.723 kb on + strand, within Pf1N1B4_4685 at 5175.723 kb on + strand, within Pf1N1B4_4685 at 5175.724 kb on - strand, within Pf1N1B4_4685 at 5176.062 kb on + strand, within Pf1N1B4_4685 at 5176.062 kb on + strand, within Pf1N1B4_4685 at 5176.063 kb on - strand, within Pf1N1B4_4685 at 5176.126 kb on - strand, within Pf1N1B4_4685 at 5176.186 kb on - strand, within Pf1N1B4_4685 at 5176.290 kb on + strand, within Pf1N1B4_4685 at 5176.291 kb on - strand, within Pf1N1B4_4685 at 5176.517 kb on + strand at 5176.517 kb on + strand at 5176.823 kb on + strand, within Pf1N1B4_4686 at 5176.823 kb on + strand, within Pf1N1B4_4686 at 5176.823 kb on + strand, within Pf1N1B4_4686 at 5176.823 kb on + strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand at 5177.351 kb on + strand, within Pf1N1B4_4686 at 5177.351 kb on + strand, within Pf1N1B4_4686
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 5,173,720 + Pf1N1B4_4684 0.73 -0.0 5,173,720 + -0.0 5,173,720 + Pf1N1B4_4684 0.73 -0.0 5,173,720 + Pf1N1B4_4684 0.73 -0.0 5,173,721 - Pf1N1B4_4684 0.73 -0.9 5,173,721 - Pf1N1B4_4684 0.73 -0.0 5,173,835 + Pf1N1B4_4684 0.77 -3.3 5,174,342 - -2.6 5,174,371 + -3.4 5,174,947 + Pf1N1B4_4685 0.23 -0.0 5,175,111 + Pf1N1B4_4685 0.31 -2.4 5,175,117 + Pf1N1B4_4685 0.32 -2.7 5,175,499 - -0.0 5,175,723 + Pf1N1B4_4685 0.61 -2.9 5,175,723 + Pf1N1B4_4685 0.61 -3.5 5,175,724 - Pf1N1B4_4685 0.61 -3.2 5,176,062 + Pf1N1B4_4685 0.77 -0.0 5,176,062 + Pf1N1B4_4685 0.77 -0.0 5,176,063 - Pf1N1B4_4685 0.77 -4.4 5,176,126 - Pf1N1B4_4685 0.80 -0.0 5,176,186 - Pf1N1B4_4685 0.83 -3.8 5,176,290 + Pf1N1B4_4685 0.88 -3.6 5,176,291 - Pf1N1B4_4685 0.88 -0.0 5,176,517 + -1.8 5,176,517 + -0.0 5,176,823 + Pf1N1B4_4686 0.16 -0.0 5,176,823 + Pf1N1B4_4686 0.16 -0.0 5,176,823 + Pf1N1B4_4686 0.16 -2.1 5,176,823 + Pf1N1B4_4686 0.16 -0.0 5,176,824 - Pf1N1B4_4686 0.16 -0.0 5,176,824 - Pf1N1B4_4686 0.16 -0.0 5,176,824 - Pf1N1B4_4686 0.16 -2.8 5,176,824 - Pf1N1B4_4686 0.16 -1.7 5,176,824 - Pf1N1B4_4686 0.16 -0.0 5,176,824 - Pf1N1B4_4686 0.16 -0.0 5,176,824 - -0.0 5,177,351 + Pf1N1B4_4686 0.46 -0.0 5,177,351 + Pf1N1B4_4686 0.46 -0.0
Or see this region's nucleotide sequence