Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4531

Experiment: Nitrate reduction, replicate C t3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4529 and Pf1N1B4_4530 are separated by 336 nucleotidesPf1N1B4_4530 and Pf1N1B4_4531 are separated by 12 nucleotides Pf1N1B4_4529 - FIG00966904: hypothetical protein, at 4,993,208 to 4,993,603 _4529 Pf1N1B4_4530 - Phophatidylinositol-4-phosphate 5-kinase (EC 2.7.1.68), at 4,993,940 to 4,994,482 _4530 Pf1N1B4_4531 - FIG01221261: hypothetical protein, at 4,994,495 to 4,996,504 _4531 Position (kb) 4994 4995 4996 4997Strain fitness (log2 ratio) -3 -2 -1 0 1at 4993.739 kb on + strandat 4993.868 kb on - strandat 4993.868 kb on - strandat 4994.143 kb on - strand, within Pf1N1B4_4530at 4994.143 kb on - strand, within Pf1N1B4_4530at 4994.179 kb on - strand, within Pf1N1B4_4530at 4994.322 kb on + strand, within Pf1N1B4_4530at 4994.656 kb on + strandat 4994.657 kb on - strandat 4994.817 kb on + strand, within Pf1N1B4_4531at 4995.726 kb on + strand, within Pf1N1B4_4531at 4995.745 kb on - strand, within Pf1N1B4_4531at 4995.783 kb on + strandat 4995.813 kb on + strand, within Pf1N1B4_4531at 4996.086 kb on + strand, within Pf1N1B4_4531at 4996.087 kb on - strand, within Pf1N1B4_4531at 4996.549 kb on + strandat 4996.829 kb on - strandat 4996.986 kb on - strandat 4997.210 kb on + strandat 4997.370 kb on - strandat 4997.408 kb on + strandat 4997.453 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3
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4,993,739 + -0.0
4,993,868 - -2.9
4,993,868 - -3.0
4,994,143 - Pf1N1B4_4530 0.37 -2.5
4,994,143 - Pf1N1B4_4530 0.37 -0.0
4,994,179 - Pf1N1B4_4530 0.44 -0.0
4,994,322 + Pf1N1B4_4530 0.70 -0.0
4,994,656 + -0.0
4,994,657 - -0.0
4,994,817 + Pf1N1B4_4531 0.16 -3.1
4,995,726 + Pf1N1B4_4531 0.61 -3.5
4,995,745 - Pf1N1B4_4531 0.62 -2.5
4,995,783 + -0.6
4,995,813 + Pf1N1B4_4531 0.66 -0.0
4,996,086 + Pf1N1B4_4531 0.79 -1.0
4,996,087 - Pf1N1B4_4531 0.79 -3.7
4,996,549 + -0.0
4,996,829 - -0.0
4,996,986 - -0.0
4,997,210 + -0.0
4,997,370 - -0.0
4,997,408 + -1.6
4,997,453 - -2.7

Or see this region's nucleotide sequence