Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_44

Experiment: Nitrate reduction, replicate C t3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_43 and Pf1N1B4_44 are separated by 145 nucleotidesPf1N1B4_44 and Pf1N1B4_45 are separated by 10 nucleotidesPf1N1B4_45 and Pf1N1B4_46 are separated by 55 nucleotides Pf1N1B4_43 - hypothetical protein, at 50,934 to 51,638 _43 Pf1N1B4_44 - Multicopper oxidase, at 51,784 to 53,577 _44 Pf1N1B4_45 - Copper resistance protein B, at 53,588 to 54,478 _45 Pf1N1B4_46 - Copper resistance protein C precursor, at 54,534 to 54,917 _46 Position (kb) 51 52 53 54Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 50.841 kb on + strandat 50.904 kb on - strandat 50.959 kb on + strandat 50.987 kb on + strandat 50.987 kb on + strandat 50.987 kb on + strandat 51.128 kb on - strandat 51.142 kb on - strand, within Pf1N1B4_43at 51.318 kb on + strand, within Pf1N1B4_43at 51.318 kb on + strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.417 kb on + strand, within Pf1N1B4_43at 51.418 kb on - strandat 51.418 kb on - strand, within Pf1N1B4_43at 51.543 kb on + strand, within Pf1N1B4_43at 51.543 kb on + strand, within Pf1N1B4_43at 51.544 kb on - strand, within Pf1N1B4_43at 51.579 kb on + strandat 51.580 kb on - strandat 51.580 kb on - strandat 51.580 kb on - strandat 51.580 kb on - strandat 51.580 kb on - strandat 51.580 kb on - strandat 51.581 kb on + strandat 51.582 kb on - strandat 51.582 kb on - strandat 51.582 kb on - strandat 51.582 kb on - strandat 51.664 kb on + strandat 51.665 kb on - strandat 51.716 kb on + strandat 51.716 kb on + strandat 51.716 kb on + strandat 51.717 kb on - strandat 51.717 kb on - strandat 51.717 kb on - strandat 51.717 kb on - strandat 51.717 kb on - strandat 52.145 kb on + strand, within Pf1N1B4_44at 52.146 kb on - strand, within Pf1N1B4_44at 52.146 kb on - strand, within Pf1N1B4_44at 52.146 kb on - strand, within Pf1N1B4_44at 52.146 kb on - strand, within Pf1N1B4_44at 52.162 kb on + strand, within Pf1N1B4_44at 52.162 kb on + strand, within Pf1N1B4_44at 52.162 kb on + strand, within Pf1N1B4_44at 52.162 kb on + strand, within Pf1N1B4_44at 52.162 kb on + strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strandat 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.168 kb on + strand, within Pf1N1B4_44at 52.169 kb on - strand, within Pf1N1B4_44at 52.169 kb on - strand, within Pf1N1B4_44at 52.169 kb on - strand, within Pf1N1B4_44at 52.198 kb on + strand, within Pf1N1B4_44at 52.198 kb on + strand, within Pf1N1B4_44at 52.198 kb on + strand, within Pf1N1B4_44at 52.198 kb on + strand, within Pf1N1B4_44at 52.199 kb on - strand, within Pf1N1B4_44at 52.199 kb on - strand, within Pf1N1B4_44at 52.199 kb on - strand, within Pf1N1B4_44at 52.199 kb on - strand, within Pf1N1B4_44at 52.300 kb on + strand, within Pf1N1B4_44at 52.300 kb on + strand, within Pf1N1B4_44at 52.379 kb on - strand, within Pf1N1B4_44at 52.379 kb on - strand, within Pf1N1B4_44at 52.385 kb on - strand, within Pf1N1B4_44at 52.385 kb on - strand, within Pf1N1B4_44at 52.385 kb on - strand, within Pf1N1B4_44at 52.519 kb on + strand, within Pf1N1B4_44at 52.519 kb on + strand, within Pf1N1B4_44at 52.519 kb on + strand, within Pf1N1B4_44at 52.520 kb on - strand, within Pf1N1B4_44at 52.520 kb on - strand, within Pf1N1B4_44at 52.520 kb on - strand, within Pf1N1B4_44at 52.525 kb on + strandat 52.525 kb on + strand, within Pf1N1B4_44at 52.525 kb on + strand, within Pf1N1B4_44at 52.525 kb on + strandat 52.525 kb on + strand, within Pf1N1B4_44at 52.525 kb on + strand, within Pf1N1B4_44at 52.525 kb on + strand, within Pf1N1B4_44at 52.525 kb on + strand, within Pf1N1B4_44at 52.526 kb on - strand, within Pf1N1B4_44at 52.526 kb on - strand, within Pf1N1B4_44at 52.526 kb on - strand, within Pf1N1B4_44at 52.526 kb on - strand, within Pf1N1B4_44at 52.529 kb on + strand, within Pf1N1B4_44at 52.529 kb on + strand, within Pf1N1B4_44at 52.530 kb on - strand, within Pf1N1B4_44at 52.530 kb on - strand, within Pf1N1B4_44at 52.530 kb on - strand, within Pf1N1B4_44at 52.624 kb on + strand, within Pf1N1B4_44at 52.765 kb on + strandat 52.765 kb on + strand, within Pf1N1B4_44at 52.765 kb on + strand, within Pf1N1B4_44at 52.766 kb on - strand, within Pf1N1B4_44at 52.766 kb on - strand, within Pf1N1B4_44at 53.177 kb on - strand, within Pf1N1B4_44at 53.512 kb on + strandat 53.531 kb on - strandat 53.689 kb on + strand, within Pf1N1B4_45at 53.816 kb on + strandat 53.861 kb on + strand, within Pf1N1B4_45at 53.861 kb on + strand, within Pf1N1B4_45at 53.861 kb on + strandat 53.861 kb on + strandat 53.861 kb on + strand, within Pf1N1B4_45at 53.861 kb on + strandat 53.862 kb on - strandat 53.862 kb on - strand, within Pf1N1B4_45at 53.864 kb on + strand, within Pf1N1B4_45at 53.864 kb on + strand, within Pf1N1B4_45at 53.864 kb on + strand, within Pf1N1B4_45at 53.864 kb on + strand, within Pf1N1B4_45at 54.137 kb on + strand, within Pf1N1B4_45at 54.138 kb on - strand, within Pf1N1B4_45at 54.210 kb on - strand, within Pf1N1B4_45at 54.365 kb on + strandat 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.366 kb on - strand, within Pf1N1B4_45at 54.366 kb on - strand, within Pf1N1B4_45at 54.366 kb on - strand, within Pf1N1B4_45at 54.393 kb on - strandat 54.410 kb on + strandat 54.411 kb on - strandat 54.485 kb on + strandat 54.486 kb on - strandat 54.514 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3
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50,841 + -0.0
50,904 - -0.0
50,959 + -0.0
50,987 + -3.3
50,987 + -0.0
50,987 + -3.0
51,128 - -0.0
51,142 - Pf1N1B4_43 0.30 -0.0
51,318 + Pf1N1B4_43 0.54 -0.0
51,318 + Pf1N1B4_43 0.54 -0.0
51,319 - Pf1N1B4_43 0.55 -5.9
51,319 - Pf1N1B4_43 0.55 -0.0
51,319 - Pf1N1B4_43 0.55 -0.0
51,319 - Pf1N1B4_43 0.55 -2.8
51,319 - Pf1N1B4_43 0.55 -0.0
51,319 - Pf1N1B4_43 0.55 -0.0
51,319 - Pf1N1B4_43 0.55 -0.0
51,319 - Pf1N1B4_43 0.55 -4.5
51,319 - Pf1N1B4_43 0.55 -1.6
51,319 - Pf1N1B4_43 0.55 -4.4
51,417 + Pf1N1B4_43 0.69 -0.0
51,418 - -0.0
51,418 - Pf1N1B4_43 0.69 -3.0
51,543 + Pf1N1B4_43 0.86 -3.6
51,543 + Pf1N1B4_43 0.86 -1.3
51,544 - Pf1N1B4_43 0.87 -0.0
51,579 + -1.6
51,580 - -3.8
51,580 - -0.0
51,580 - -0.0
51,580 - -3.7
51,580 - -0.0
51,580 - -0.0
51,581 + -4.0
51,582 - -4.2
51,582 - -3.8
51,582 - -0.0
51,582 - -0.0
51,664 + -3.8
51,665 - -0.0
51,716 + -3.3
51,716 + -0.0
51,716 + -2.4
51,717 - -2.1
51,717 - -0.0
51,717 - -2.8
51,717 - -0.0
51,717 - -0.0
52,145 + Pf1N1B4_44 0.20 -1.6
52,146 - Pf1N1B4_44 0.20 -0.0
52,146 - Pf1N1B4_44 0.20 -2.3
52,146 - Pf1N1B4_44 0.20 -0.0
52,146 - Pf1N1B4_44 0.20 -0.0
52,162 + Pf1N1B4_44 0.21 -0.0
52,162 + Pf1N1B4_44 0.21 -0.0
52,162 + Pf1N1B4_44 0.21 -2.8
52,162 + Pf1N1B4_44 0.21 -2.6
52,162 + Pf1N1B4_44 0.21 -2.1
52,163 - Pf1N1B4_44 0.21 -0.0
52,163 - -2.5
52,163 - Pf1N1B4_44 0.21 -2.4
52,163 - Pf1N1B4_44 0.21 -0.0
52,163 - Pf1N1B4_44 0.21 -0.0
52,163 - Pf1N1B4_44 0.21 -0.0
52,163 - Pf1N1B4_44 0.21 -0.0
52,163 - Pf1N1B4_44 0.21 -0.0
52,163 - Pf1N1B4_44 0.21 -3.4
52,163 - Pf1N1B4_44 0.21 -5.1
52,163 - Pf1N1B4_44 0.21 -3.3
52,163 - Pf1N1B4_44 0.21 -2.7
52,163 - Pf1N1B4_44 0.21 -0.0
52,163 - Pf1N1B4_44 0.21 -5.1
52,163 - Pf1N1B4_44 0.21 -0.0
52,163 - Pf1N1B4_44 0.21 -0.0
52,168 + Pf1N1B4_44 0.21 -2.2
52,169 - Pf1N1B4_44 0.21 -0.0
52,169 - Pf1N1B4_44 0.21 -0.0
52,169 - Pf1N1B4_44 0.21 -0.0
52,198 + Pf1N1B4_44 0.23 -0.0
52,198 + Pf1N1B4_44 0.23 -0.0
52,198 + Pf1N1B4_44 0.23 -0.0
52,198 + Pf1N1B4_44 0.23 -0.0
52,199 - Pf1N1B4_44 0.23 -0.0
52,199 - Pf1N1B4_44 0.23 -0.0
52,199 - Pf1N1B4_44 0.23 -0.0
52,199 - Pf1N1B4_44 0.23 -0.0
52,300 + Pf1N1B4_44 0.29 -1.0
52,300 + Pf1N1B4_44 0.29 -0.0
52,379 - Pf1N1B4_44 0.33 -3.3
52,379 - Pf1N1B4_44 0.33 -1.9
52,385 - Pf1N1B4_44 0.34 -0.0
52,385 - Pf1N1B4_44 0.34 -4.5
52,385 - Pf1N1B4_44 0.34 -0.0
52,519 + Pf1N1B4_44 0.41 -0.0
52,519 + Pf1N1B4_44 0.41 -3.3
52,519 + Pf1N1B4_44 0.41 -0.0
52,520 - Pf1N1B4_44 0.41 -0.0
52,520 - Pf1N1B4_44 0.41 -0.0
52,520 - Pf1N1B4_44 0.41 -0.0
52,525 + -4.7
52,525 + Pf1N1B4_44 0.41 -2.1
52,525 + Pf1N1B4_44 0.41 -2.1
52,525 + -0.0
52,525 + Pf1N1B4_44 0.41 -0.0
52,525 + Pf1N1B4_44 0.41 -0.0
52,525 + Pf1N1B4_44 0.41 -3.3
52,525 + Pf1N1B4_44 0.41 -1.9
52,526 - Pf1N1B4_44 0.41 -0.0
52,526 - Pf1N1B4_44 0.41 -0.0
52,526 - Pf1N1B4_44 0.41 -0.0
52,526 - Pf1N1B4_44 0.41 -0.0
52,529 + Pf1N1B4_44 0.42 -2.3
52,529 + Pf1N1B4_44 0.42 -2.0
52,530 - Pf1N1B4_44 0.42 -0.0
52,530 - Pf1N1B4_44 0.42 -0.0
52,530 - Pf1N1B4_44 0.42 -0.0
52,624 + Pf1N1B4_44 0.47 -2.5
52,765 + -2.7
52,765 + Pf1N1B4_44 0.55 -0.0
52,765 + Pf1N1B4_44 0.55 -0.0
52,766 - Pf1N1B4_44 0.55 -0.0
52,766 - Pf1N1B4_44 0.55 -1.6
53,177 - Pf1N1B4_44 0.78 -0.0
53,512 + -1.7
53,531 - -1.6
53,689 + Pf1N1B4_45 0.11 -2.3
53,816 + -3.9
53,861 + Pf1N1B4_45 0.31 -1.0
53,861 + Pf1N1B4_45 0.31 -3.4
53,861 + -4.6
53,861 + -2.2
53,861 + Pf1N1B4_45 0.31 -0.0
53,861 + -4.2
53,862 - -0.0
53,862 - Pf1N1B4_45 0.31 -2.7
53,864 + Pf1N1B4_45 0.31 -1.8
53,864 + Pf1N1B4_45 0.31 -4.3
53,864 + Pf1N1B4_45 0.31 -0.0
53,864 + Pf1N1B4_45 0.31 -0.0
54,137 + Pf1N1B4_45 0.62 -2.7
54,138 - Pf1N1B4_45 0.62 -0.0
54,210 - Pf1N1B4_45 0.70 -5.5
54,365 + -2.8
54,365 + Pf1N1B4_45 0.87 -0.0
54,365 + Pf1N1B4_45 0.87 -1.9
54,365 + Pf1N1B4_45 0.87 -0.0
54,365 + Pf1N1B4_45 0.87 -0.0
54,365 + Pf1N1B4_45 0.87 -0.0
54,365 + Pf1N1B4_45 0.87 -1.8
54,366 - Pf1N1B4_45 0.87 -0.0
54,366 - Pf1N1B4_45 0.87 -1.6
54,366 - Pf1N1B4_45 0.87 -3.0
54,393 - -0.0
54,410 + -3.1
54,411 - -0.0
54,485 + -1.9
54,486 - -0.0
54,514 - -2.9

Or see this region's nucleotide sequence