Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4021 and Pf1N1B4_4022 are separated by 221 nucleotides Pf1N1B4_4022 and Pf1N1B4_4023 are separated by 79 nucleotides Pf1N1B4_4023 and Pf1N1B4_4024 are separated by 477 nucleotides
Pf1N1B4_4021 - Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA, at 4,474,077 to 4,474,733
_4021
Pf1N1B4_4022 - hypothetical protein, at 4,474,955 to 4,475,155
_4022
Pf1N1B4_4023 - hypothetical protein, at 4,475,235 to 4,475,567
_4023
Pf1N1B4_4024 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 4,476,045 to 4,476,425
_4024
Position (kb) 4475
4476 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4474.568 kb on + strand, within Pf1N1B4_4021 at 4474.686 kb on + strand at 4474.723 kb on + strand at 4474.727 kb on + strand at 4474.727 kb on + strand at 4474.727 kb on + strand at 4474.727 kb on + strand at 4474.727 kb on + strand at 4474.727 kb on + strand at 4474.727 kb on + strand at 4474.727 kb on + strand at 4474.728 kb on - strand at 4474.731 kb on + strand at 4474.731 kb on + strand at 4474.731 kb on + strand at 4474.831 kb on + strand at 4474.832 kb on - strand at 4474.835 kb on + strand at 4474.835 kb on + strand at 4474.836 kb on - strand at 4474.869 kb on - strand at 4474.875 kb on + strand at 4474.876 kb on - strand at 4474.876 kb on - strand at 4474.879 kb on + strand at 4474.879 kb on + strand at 4474.879 kb on + strand at 4474.879 kb on + strand at 4474.880 kb on - strand at 4474.880 kb on - strand at 4474.880 kb on - strand at 4474.916 kb on + strand at 4474.917 kb on - strand at 4474.935 kb on - strand at 4474.938 kb on + strand at 4474.938 kb on + strand at 4474.938 kb on + strand at 4475.143 kb on + strand at 4475.153 kb on + strand at 4475.153 kb on + strand at 4475.153 kb on + strand at 4475.153 kb on + strand at 4475.153 kb on + strand at 4475.154 kb on - strand at 4475.234 kb on + strand at 4475.235 kb on - strand at 4475.235 kb on - strand at 4475.340 kb on - strand, within Pf1N1B4_4023 at 4475.361 kb on + strand, within Pf1N1B4_4023 at 4475.480 kb on + strand, within Pf1N1B4_4023 at 4475.493 kb on + strand, within Pf1N1B4_4023 at 4475.493 kb on + strand, within Pf1N1B4_4023 at 4475.493 kb on + strand, within Pf1N1B4_4023 at 4475.493 kb on + strand, within Pf1N1B4_4023 at 4475.516 kb on + strand, within Pf1N1B4_4023 at 4475.516 kb on + strand, within Pf1N1B4_4023 at 4475.516 kb on + strand, within Pf1N1B4_4023 at 4475.530 kb on - strand, within Pf1N1B4_4023 at 4475.530 kb on - strand, within Pf1N1B4_4023 at 4475.693 kb on + strand at 4475.704 kb on + strand at 4475.704 kb on + strand at 4475.705 kb on - strand at 4475.741 kb on + strand at 4475.741 kb on + strand at 4475.742 kb on - strand at 4475.875 kb on + strand at 4475.875 kb on + strand at 4475.875 kb on + strand at 4475.875 kb on + strand at 4475.875 kb on + strand at 4475.875 kb on + strand at 4475.876 kb on - strand at 4475.876 kb on - strand at 4475.876 kb on - strand at 4475.876 kb on - strand at 4475.901 kb on - strand at 4475.978 kb on - strand at 4475.978 kb on - strand at 4476.024 kb on + strand at 4476.165 kb on + strand, within Pf1N1B4_4024 at 4476.166 kb on - strand, within Pf1N1B4_4024 at 4476.168 kb on + strand, within Pf1N1B4_4024 at 4476.168 kb on + strand, within Pf1N1B4_4024 at 4476.168 kb on + strand, within Pf1N1B4_4024 at 4476.169 kb on - strand, within Pf1N1B4_4024 at 4476.169 kb on - strand at 4476.423 kb on + strand at 4476.423 kb on + strand at 4476.423 kb on + strand at 4476.424 kb on - strand at 4476.543 kb on + strand at 4476.544 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 4,474,568 + Pf1N1B4_4021 0.75 +0.0 4,474,686 + +0.0 4,474,723 + -2.3 4,474,727 + -2.1 4,474,727 + +0.0 4,474,727 + +0.0 4,474,727 + +0.0 4,474,727 + -4.0 4,474,727 + -2.9 4,474,727 + -3.2 4,474,727 + -1.9 4,474,728 - -1.9 4,474,731 + -1.6 4,474,731 + +0.0 4,474,731 + -3.5 4,474,831 + -2.4 4,474,832 - +0.0 4,474,835 + -2.1 4,474,835 + +0.0 4,474,836 - -3.1 4,474,869 - -2.7 4,474,875 + -2.3 4,474,876 - +0.0 4,474,876 - +0.0 4,474,879 + +0.0 4,474,879 + +0.0 4,474,879 + +0.0 4,474,879 + -0.9 4,474,880 - +0.0 4,474,880 - -3.6 4,474,880 - +0.0 4,474,916 + +0.0 4,474,917 - +0.0 4,474,935 - +0.0 4,474,938 + +0.0 4,474,938 + +0.0 4,474,938 + +0.0 4,475,143 + +0.0 4,475,153 + +0.0 4,475,153 + -2.6 4,475,153 + +0.0 4,475,153 + -1.0 4,475,153 + +0.0 4,475,154 - -2.8 4,475,234 + -2.5 4,475,235 - +0.0 4,475,235 - +0.0 4,475,340 - Pf1N1B4_4023 0.32 +0.0 4,475,361 + Pf1N1B4_4023 0.38 +0.0 4,475,480 + Pf1N1B4_4023 0.74 -3.9 4,475,493 + Pf1N1B4_4023 0.77 +0.0 4,475,493 + Pf1N1B4_4023 0.77 +0.0 4,475,493 + Pf1N1B4_4023 0.77 -3.0 4,475,493 + Pf1N1B4_4023 0.77 +0.0 4,475,516 + Pf1N1B4_4023 0.84 -3.4 4,475,516 + Pf1N1B4_4023 0.84 -2.6 4,475,516 + Pf1N1B4_4023 0.84 +0.0 4,475,530 - Pf1N1B4_4023 0.89 +0.0 4,475,530 - Pf1N1B4_4023 0.89 -2.4 4,475,693 + -2.4 4,475,704 + +0.0 4,475,704 + +0.0 4,475,705 - -4.7 4,475,741 + -1.0 4,475,741 + +0.0 4,475,742 - +0.0 4,475,875 + +0.0 4,475,875 + +0.0 4,475,875 + -2.9 4,475,875 + -5.6 4,475,875 + +0.0 4,475,875 + -2.8 4,475,876 - +0.0 4,475,876 - +0.0 4,475,876 - +0.0 4,475,876 - -3.5 4,475,901 - -2.6 4,475,978 - +0.0 4,475,978 - -3.3 4,476,024 + +0.0 4,476,165 + Pf1N1B4_4024 0.31 -3.0 4,476,166 - Pf1N1B4_4024 0.32 -3.6 4,476,168 + Pf1N1B4_4024 0.32 +0.0 4,476,168 + Pf1N1B4_4024 0.32 +0.0 4,476,168 + Pf1N1B4_4024 0.32 +0.0 4,476,169 - Pf1N1B4_4024 0.33 +0.0 4,476,169 - +0.0 4,476,423 + +0.0 4,476,423 + +0.0 4,476,423 + +0.0 4,476,424 - -3.3 4,476,543 + -2.2 4,476,544 - +0.0
Or see this region's nucleotide sequence