Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_396

Experiment: Nitrate reduction, replicate C t3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_395 and Pf1N1B4_396 overlap by 4 nucleotidesPf1N1B4_396 and Pf1N1B4_397 are separated by 60 nucleotides Pf1N1B4_395 - Alcohol dehydrogenase (EC 1.1.1.1), at 464,129 to 465,181 _395 Pf1N1B4_396 - D-galactonate regulator, IclR family, at 465,178 to 465,981 _396 Pf1N1B4_397 - Hexuronate transporter, at 466,042 to 467,352 _397 Position (kb) 465 466Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 464.297 kb on + strand, within Pf1N1B4_395at 464.298 kb on - strand, within Pf1N1B4_395at 464.356 kb on + strand, within Pf1N1B4_395at 464.359 kb on + strandat 464.359 kb on + strand, within Pf1N1B4_395at 464.359 kb on + strand, within Pf1N1B4_395at 464.359 kb on + strand, within Pf1N1B4_395at 464.360 kb on - strand, within Pf1N1B4_395at 464.360 kb on - strand, within Pf1N1B4_395at 464.360 kb on - strand, within Pf1N1B4_395at 464.448 kb on - strand, within Pf1N1B4_395at 464.474 kb on + strand, within Pf1N1B4_395at 464.474 kb on + strand, within Pf1N1B4_395at 464.475 kb on - strand, within Pf1N1B4_395at 464.475 kb on - strand, within Pf1N1B4_395at 464.621 kb on + strand, within Pf1N1B4_395at 464.622 kb on - strand, within Pf1N1B4_395at 464.639 kb on + strand, within Pf1N1B4_395at 464.640 kb on - strand, within Pf1N1B4_395at 464.919 kb on + strandat 464.919 kb on + strand, within Pf1N1B4_395at 464.919 kb on + strand, within Pf1N1B4_395at 464.920 kb on - strand, within Pf1N1B4_395at 464.920 kb on - strand, within Pf1N1B4_395at 465.113 kb on + strandat 465.113 kb on + strandat 465.113 kb on + strandat 465.113 kb on + strandat 465.113 kb on + strandat 465.113 kb on + strandat 465.114 kb on - strandat 465.114 kb on - strandat 465.114 kb on - strandat 465.114 kb on - strandat 465.114 kb on - strandat 465.114 kb on - strandat 465.114 kb on - strandat 465.143 kb on + strandat 465.144 kb on - strandat 465.144 kb on - strandat 465.144 kb on - strandat 465.180 kb on - strandat 465.266 kb on + strand, within Pf1N1B4_396at 465.266 kb on + strand, within Pf1N1B4_396at 465.266 kb on + strand, within Pf1N1B4_396at 465.267 kb on - strand, within Pf1N1B4_396at 465.267 kb on - strand, within Pf1N1B4_396at 465.393 kb on - strand, within Pf1N1B4_396at 465.393 kb on - strand, within Pf1N1B4_396at 465.486 kb on - strand, within Pf1N1B4_396at 465.617 kb on + strand, within Pf1N1B4_396at 465.618 kb on - strand, within Pf1N1B4_396at 465.767 kb on + strand, within Pf1N1B4_396at 465.794 kb on + strand, within Pf1N1B4_396at 465.794 kb on + strand, within Pf1N1B4_396at 465.795 kb on - strand, within Pf1N1B4_396at 465.835 kb on - strand, within Pf1N1B4_396at 466.021 kb on - strandat 466.021 kb on - strandat 466.044 kb on - strandat 466.054 kb on - strandat 466.082 kb on + strandat 466.083 kb on - strandat 466.116 kb on + strandat 466.116 kb on + strandat 466.117 kb on - strandat 466.117 kb on - strandat 466.117 kb on - strandat 466.117 kb on - strandat 466.117 kb on - strandat 466.117 kb on - strandat 466.118 kb on + strandat 466.118 kb on + strandat 466.118 kb on + strandat 466.118 kb on + strandat 466.118 kb on + strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.119 kb on - strandat 466.123 kb on - strandat 466.179 kb on + strand, within Pf1N1B4_397at 466.541 kb on + strand, within Pf1N1B4_397at 466.542 kb on - strand, within Pf1N1B4_397at 466.542 kb on - strand, within Pf1N1B4_397at 466.601 kb on + strand, within Pf1N1B4_397at 466.601 kb on + strand, within Pf1N1B4_397at 466.602 kb on - strand, within Pf1N1B4_397at 466.659 kb on + strand, within Pf1N1B4_397at 466.660 kb on - strand, within Pf1N1B4_397at 466.904 kb on + strand, within Pf1N1B4_397at 466.904 kb on + strand, within Pf1N1B4_397at 466.904 kb on + strand, within Pf1N1B4_397at 466.904 kb on + strand, within Pf1N1B4_397at 466.905 kb on - strand, within Pf1N1B4_397at 466.905 kb on - strand, within Pf1N1B4_397at 466.974 kb on - strand, within Pf1N1B4_397

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3
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464,297 + Pf1N1B4_395 0.16 -5.0
464,298 - Pf1N1B4_395 0.16 -3.1
464,356 + Pf1N1B4_395 0.22 -2.3
464,359 + -0.1
464,359 + Pf1N1B4_395 0.22 -0.1
464,359 + Pf1N1B4_395 0.22 -2.2
464,359 + Pf1N1B4_395 0.22 -2.5
464,360 - Pf1N1B4_395 0.22 -0.1
464,360 - Pf1N1B4_395 0.22 -0.1
464,360 - Pf1N1B4_395 0.22 -3.4
464,448 - Pf1N1B4_395 0.30 -3.3
464,474 + Pf1N1B4_395 0.33 -0.1
464,474 + Pf1N1B4_395 0.33 -0.1
464,475 - Pf1N1B4_395 0.33 -3.1
464,475 - Pf1N1B4_395 0.33 -3.3
464,621 + Pf1N1B4_395 0.47 -2.5
464,622 - Pf1N1B4_395 0.47 -0.1
464,639 + Pf1N1B4_395 0.48 -0.1
464,640 - Pf1N1B4_395 0.49 -0.1
464,919 + -4.4
464,919 + Pf1N1B4_395 0.75 -2.6
464,919 + Pf1N1B4_395 0.75 -0.1
464,920 - Pf1N1B4_395 0.75 -2.5
464,920 - Pf1N1B4_395 0.75 -0.1
465,113 + -0.1
465,113 + -3.1
465,113 + -0.1
465,113 + -0.1
465,113 + -0.1
465,113 + -1.6
465,114 - -0.1
465,114 - -0.1
465,114 - -0.1
465,114 - -2.3
465,114 - -2.3
465,114 - -2.9
465,114 - -0.1
465,143 + -0.1
465,144 - -3.9
465,144 - -2.5
465,144 - -1.6
465,180 - -1.4
465,266 + Pf1N1B4_396 0.11 -2.8
465,266 + Pf1N1B4_396 0.11 -0.1
465,266 + Pf1N1B4_396 0.11 -0.1
465,267 - Pf1N1B4_396 0.11 -0.1
465,267 - Pf1N1B4_396 0.11 -4.0
465,393 - Pf1N1B4_396 0.27 -1.6
465,393 - Pf1N1B4_396 0.27 -3.3
465,486 - Pf1N1B4_396 0.38 -2.6
465,617 + Pf1N1B4_396 0.55 -3.1
465,618 - Pf1N1B4_396 0.55 -3.1
465,767 + Pf1N1B4_396 0.73 -3.6
465,794 + Pf1N1B4_396 0.77 -0.1
465,794 + Pf1N1B4_396 0.77 -0.1
465,795 - Pf1N1B4_396 0.77 -1.9
465,835 - Pf1N1B4_396 0.82 -0.1
466,021 - -0.1
466,021 - -2.7
466,044 - -0.1
466,054 - -2.4
466,082 + -0.1
466,083 - -0.1
466,116 + -0.1
466,116 + -2.3
466,117 - -0.1
466,117 - -2.8
466,117 - -0.1
466,117 - -0.1
466,117 - -0.1
466,117 - -1.9
466,118 + -4.6
466,118 + -2.7
466,118 + -3.1
466,118 + -0.1
466,118 + -0.1
466,119 - -0.1
466,119 - -0.1
466,119 - -0.1
466,119 - -0.1
466,119 - -3.7
466,119 - -1.7
466,119 - -3.0
466,119 - -0.1
466,119 - -0.1
466,119 - -0.1
466,119 - -0.1
466,119 - -3.1
466,119 - -0.1
466,119 - -0.1
466,119 - -0.1
466,119 - -1.8
466,119 - -0.1
466,119 - -6.0
466,119 - -3.1
466,119 - -0.1
466,119 - -0.1
466,119 - -0.1
466,123 - -0.1
466,179 + Pf1N1B4_397 0.10 -1.4
466,541 + Pf1N1B4_397 0.38 -3.6
466,542 - Pf1N1B4_397 0.38 -0.1
466,542 - Pf1N1B4_397 0.38 -0.1
466,601 + Pf1N1B4_397 0.43 -0.1
466,601 + Pf1N1B4_397 0.43 -0.1
466,602 - Pf1N1B4_397 0.43 -3.2
466,659 + Pf1N1B4_397 0.47 -1.8
466,660 - Pf1N1B4_397 0.47 -0.1
466,904 + Pf1N1B4_397 0.66 -0.1
466,904 + Pf1N1B4_397 0.66 -0.1
466,904 + Pf1N1B4_397 0.66 -0.1
466,904 + Pf1N1B4_397 0.66 -0.1
466,905 - Pf1N1B4_397 0.66 -2.3
466,905 - Pf1N1B4_397 0.66 -2.2
466,974 - Pf1N1B4_397 0.71 -0.3

Or see this region's nucleotide sequence