Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3524

Experiment: Nitrate reduction, replicate C t3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3521 and Pf1N1B4_3522 are separated by 32 nucleotidesPf1N1B4_3522 and Pf1N1B4_3523 are separated by 52 nucleotidesPf1N1B4_3523 and Pf1N1B4_3524 are separated by 110 nucleotidesPf1N1B4_3524 and Pf1N1B4_3525 overlap by 4 nucleotidesPf1N1B4_3525 and Pf1N1B4_3526 are separated by 50 nucleotides Pf1N1B4_3521 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 3,927,792 to 3,929,459 _3521 Pf1N1B4_3522 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 3,929,492 to 3,929,935 _3522 Pf1N1B4_3523 - hypothetical protein, at 3,929,988 to 3,930,125 _3523 Pf1N1B4_3524 - FIG00955363: hypothetical protein, at 3,930,236 to 3,931,042 _3524 Pf1N1B4_3525 - hydrolase, alpha/beta fold family, at 3,931,039 to 3,931,893 _3525 Pf1N1B4_3526 - Glutathione S-transferase (EC 2.5.1.18), at 3,931,944 to 3,932,603 _3526 Position (kb) 3930 3931 3932Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3929.400 kb on + strandat 3929.400 kb on + strandat 3929.488 kb on - strandat 3929.569 kb on + strand, within Pf1N1B4_3522at 3929.705 kb on + strand, within Pf1N1B4_3522at 3929.705 kb on + strand, within Pf1N1B4_3522at 3929.706 kb on - strand, within Pf1N1B4_3522at 3930.133 kb on + strandat 3930.181 kb on + strandat 3930.235 kb on + strandat 3930.235 kb on + strandat 3930.235 kb on + strandat 3930.235 kb on + strandat 3930.236 kb on - strandat 3930.236 kb on - strandat 3930.236 kb on - strandat 3930.236 kb on - strandat 3930.249 kb on + strandat 3930.250 kb on - strandat 3930.250 kb on - strandat 3930.250 kb on - strandat 3930.288 kb on - strandat 3930.288 kb on - strandat 3930.321 kb on - strand, within Pf1N1B4_3524at 3930.674 kb on - strandat 3930.674 kb on - strand, within Pf1N1B4_3524at 3930.716 kb on + strand, within Pf1N1B4_3524at 3930.716 kb on + strand, within Pf1N1B4_3524at 3930.791 kb on + strand, within Pf1N1B4_3524at 3930.792 kb on - strand, within Pf1N1B4_3524at 3930.792 kb on - strand, within Pf1N1B4_3524at 3930.792 kb on - strand, within Pf1N1B4_3524at 3930.949 kb on + strand, within Pf1N1B4_3524at 3930.949 kb on + strand, within Pf1N1B4_3524at 3930.949 kb on + strand, within Pf1N1B4_3524at 3931.299 kb on - strand, within Pf1N1B4_3525at 3931.431 kb on + strand, within Pf1N1B4_3525at 3931.516 kb on + strandat 3931.516 kb on + strand, within Pf1N1B4_3525at 3931.516 kb on + strand, within Pf1N1B4_3525at 3931.629 kb on + strand, within Pf1N1B4_3525at 3931.629 kb on + strand, within Pf1N1B4_3525at 3931.630 kb on - strandat 3931.948 kb on + strandat 3931.948 kb on + strandat 3931.948 kb on + strandat 3931.949 kb on - strandat 3931.949 kb on - strandat 3931.956 kb on + strandat 3931.956 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3
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3,929,400 + -3.1
3,929,400 + -3.2
3,929,488 - -0.0
3,929,569 + Pf1N1B4_3522 0.17 -0.0
3,929,705 + Pf1N1B4_3522 0.48 -0.0
3,929,705 + Pf1N1B4_3522 0.48 -0.0
3,929,706 - Pf1N1B4_3522 0.48 -0.0
3,930,133 + -2.8
3,930,181 + -0.0
3,930,235 + -0.0
3,930,235 + -0.0
3,930,235 + -2.8
3,930,235 + -0.0
3,930,236 - -2.2
3,930,236 - -1.1
3,930,236 - -3.6
3,930,236 - -1.9
3,930,249 + -0.0
3,930,250 - -0.0
3,930,250 - -0.0
3,930,250 - -0.0
3,930,288 - -2.0
3,930,288 - -0.0
3,930,321 - Pf1N1B4_3524 0.11 -0.0
3,930,674 - -3.9
3,930,674 - Pf1N1B4_3524 0.54 -0.0
3,930,716 + Pf1N1B4_3524 0.59 -2.4
3,930,716 + Pf1N1B4_3524 0.59 -3.5
3,930,791 + Pf1N1B4_3524 0.69 -2.9
3,930,792 - Pf1N1B4_3524 0.69 -2.4
3,930,792 - Pf1N1B4_3524 0.69 -3.0
3,930,792 - Pf1N1B4_3524 0.69 -3.6
3,930,949 + Pf1N1B4_3524 0.88 -2.7
3,930,949 + Pf1N1B4_3524 0.88 -0.0
3,930,949 + Pf1N1B4_3524 0.88 -0.0
3,931,299 - Pf1N1B4_3525 0.30 -0.0
3,931,431 + Pf1N1B4_3525 0.46 -0.0
3,931,516 + -4.0
3,931,516 + Pf1N1B4_3525 0.56 -3.4
3,931,516 + Pf1N1B4_3525 0.56 -2.9
3,931,629 + Pf1N1B4_3525 0.69 -2.6
3,931,629 + Pf1N1B4_3525 0.69 -2.2
3,931,630 - -1.9
3,931,948 + -0.0
3,931,948 + -0.0
3,931,948 + -0.0
3,931,949 - -2.3
3,931,949 - -0.0
3,931,956 + -2.8
3,931,956 + -0.8

Or see this region's nucleotide sequence