Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2881

Experiment: Nitrate reduction, replicate C t3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2880 and Pf1N1B4_2881 are separated by 146 nucleotidesPf1N1B4_2881 and Pf1N1B4_2882 are separated by 63 nucleotides Pf1N1B4_2880 - Ketol-acid reductoisomerase (EC 1.1.1.86), at 3,226,732 to 3,227,748 _2880 Pf1N1B4_2881 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 3,227,895 to 3,228,752 _2881 Pf1N1B4_2882 - Putative sulfite oxidase subunit YedY, at 3,228,816 to 3,229,829 _2882 Position (kb) 3227 3228 3229Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 3226.996 kb on + strand, within Pf1N1B4_2880at 3226.996 kb on + strand, within Pf1N1B4_2880at 3226.996 kb on + strand, within Pf1N1B4_2880at 3226.996 kb on + strand, within Pf1N1B4_2880at 3226.997 kb on - strand, within Pf1N1B4_2880at 3226.997 kb on - strand, within Pf1N1B4_2880at 3226.997 kb on - strand, within Pf1N1B4_2880at 3227.068 kb on + strand, within Pf1N1B4_2880at 3227.068 kb on + strand, within Pf1N1B4_2880at 3227.068 kb on + strand, within Pf1N1B4_2880at 3227.069 kb on - strandat 3227.069 kb on - strandat 3227.185 kb on - strand, within Pf1N1B4_2880at 3227.188 kb on + strand, within Pf1N1B4_2880at 3227.188 kb on + strand, within Pf1N1B4_2880at 3227.188 kb on + strand, within Pf1N1B4_2880at 3227.188 kb on + strand, within Pf1N1B4_2880at 3227.188 kb on + strand, within Pf1N1B4_2880at 3227.188 kb on + strand, within Pf1N1B4_2880at 3227.188 kb on + strandat 3227.189 kb on - strand, within Pf1N1B4_2880at 3227.189 kb on - strand, within Pf1N1B4_2880at 3227.230 kb on + strand, within Pf1N1B4_2880at 3227.230 kb on + strand, within Pf1N1B4_2880at 3227.231 kb on - strand, within Pf1N1B4_2880at 3227.348 kb on + strand, within Pf1N1B4_2880at 3227.401 kb on + strand, within Pf1N1B4_2880at 3227.401 kb on + strand, within Pf1N1B4_2880at 3227.401 kb on + strand, within Pf1N1B4_2880at 3227.401 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.446 kb on + strand, within Pf1N1B4_2880at 3227.447 kb on - strand, within Pf1N1B4_2880at 3227.447 kb on - strand, within Pf1N1B4_2880at 3227.447 kb on - strand, within Pf1N1B4_2880at 3227.447 kb on - strand, within Pf1N1B4_2880at 3227.447 kb on - strand, within Pf1N1B4_2880at 3227.447 kb on - strand, within Pf1N1B4_2880at 3227.473 kb on + strand, within Pf1N1B4_2880at 3227.473 kb on + strand, within Pf1N1B4_2880at 3227.473 kb on + strand, within Pf1N1B4_2880at 3227.473 kb on + strand, within Pf1N1B4_2880at 3227.473 kb on + strand, within Pf1N1B4_2880at 3227.473 kb on + strand, within Pf1N1B4_2880at 3227.506 kb on + strand, within Pf1N1B4_2880at 3227.506 kb on + strand, within Pf1N1B4_2880at 3227.506 kb on + strand, within Pf1N1B4_2880at 3227.506 kb on + strand, within Pf1N1B4_2880at 3227.506 kb on + strand, within Pf1N1B4_2880at 3227.506 kb on + strand, within Pf1N1B4_2880at 3227.507 kb on - strand, within Pf1N1B4_2880at 3227.571 kb on + strand, within Pf1N1B4_2880at 3227.571 kb on + strand, within Pf1N1B4_2880at 3227.571 kb on + strand, within Pf1N1B4_2880at 3227.571 kb on + strand, within Pf1N1B4_2880at 3227.571 kb on + strand, within Pf1N1B4_2880at 3227.571 kb on + strand, within Pf1N1B4_2880at 3227.571 kb on + strand, within Pf1N1B4_2880at 3227.571 kb on + strand, within Pf1N1B4_2880at 3227.571 kb on + strand, within Pf1N1B4_2880at 3227.571 kb on + strand, within Pf1N1B4_2880at 3227.572 kb on - strand, within Pf1N1B4_2880at 3227.572 kb on - strand, within Pf1N1B4_2880at 3227.572 kb on - strand, within Pf1N1B4_2880at 3227.587 kb on + strand, within Pf1N1B4_2880at 3227.649 kb on + strandat 3227.746 kb on + strandat 3227.746 kb on + strandat 3227.746 kb on + strandat 3227.746 kb on + strandat 3227.746 kb on + strandat 3227.746 kb on + strandat 3227.746 kb on + strandat 3227.746 kb on + strandat 3227.746 kb on + strandat 3227.746 kb on + strandat 3227.746 kb on + strandat 3227.746 kb on + strandat 3227.777 kb on + strandat 3227.777 kb on + strandat 3227.777 kb on + strandat 3227.814 kb on + strandat 3227.814 kb on + strandat 3227.816 kb on + strandat 3228.898 kb on + strandat 3228.898 kb on + strandat 3228.901 kb on + strandat 3228.901 kb on + strandat 3228.901 kb on + strandat 3228.901 kb on + strandat 3229.104 kb on + strand, within Pf1N1B4_2882at 3229.104 kb on + strand, within Pf1N1B4_2882at 3229.105 kb on - strand, within Pf1N1B4_2882at 3229.253 kb on + strandat 3229.253 kb on + strand, within Pf1N1B4_2882at 3229.257 kb on + strand, within Pf1N1B4_2882at 3229.257 kb on + strand, within Pf1N1B4_2882at 3229.395 kb on - strand, within Pf1N1B4_2882at 3229.437 kb on + strand, within Pf1N1B4_2882at 3229.437 kb on + strand, within Pf1N1B4_2882at 3229.438 kb on - strand, within Pf1N1B4_2882at 3229.441 kb on + strand, within Pf1N1B4_2882at 3229.441 kb on + strand, within Pf1N1B4_2882at 3229.441 kb on + strand, within Pf1N1B4_2882at 3229.441 kb on + strand, within Pf1N1B4_2882at 3229.442 kb on - strandat 3229.442 kb on - strand, within Pf1N1B4_2882at 3229.500 kb on + strand, within Pf1N1B4_2882at 3229.500 kb on + strand, within Pf1N1B4_2882at 3229.500 kb on + strand, within Pf1N1B4_2882at 3229.500 kb on + strand, within Pf1N1B4_2882at 3229.500 kb on + strand, within Pf1N1B4_2882at 3229.500 kb on + strand, within Pf1N1B4_2882at 3229.500 kb on + strand, within Pf1N1B4_2882at 3229.500 kb on + strand, within Pf1N1B4_2882at 3229.501 kb on - strand, within Pf1N1B4_2882at 3229.501 kb on - strand, within Pf1N1B4_2882at 3229.560 kb on + strand, within Pf1N1B4_2882at 3229.560 kb on + strand, within Pf1N1B4_2882at 3229.622 kb on + strand, within Pf1N1B4_2882at 3229.622 kb on + strand, within Pf1N1B4_2882at 3229.623 kb on - strand, within Pf1N1B4_2882at 3229.660 kb on - strand, within Pf1N1B4_2882

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3
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3,226,996 + Pf1N1B4_2880 0.26 +0.0
3,226,996 + Pf1N1B4_2880 0.26 -3.3
3,226,996 + Pf1N1B4_2880 0.26 -5.7
3,226,996 + Pf1N1B4_2880 0.26 -5.6
3,226,997 - Pf1N1B4_2880 0.26 +0.0
3,226,997 - Pf1N1B4_2880 0.26 +0.0
3,226,997 - Pf1N1B4_2880 0.26 +0.0
3,227,068 + Pf1N1B4_2880 0.33 -5.6
3,227,068 + Pf1N1B4_2880 0.33 -4.7
3,227,068 + Pf1N1B4_2880 0.33 +0.0
3,227,069 - -2.3
3,227,069 - +0.0
3,227,185 - Pf1N1B4_2880 0.45 -4.4
3,227,188 + Pf1N1B4_2880 0.45 -2.3
3,227,188 + Pf1N1B4_2880 0.45 -5.8
3,227,188 + Pf1N1B4_2880 0.45 +0.0
3,227,188 + Pf1N1B4_2880 0.45 -1.6
3,227,188 + Pf1N1B4_2880 0.45 +0.0
3,227,188 + Pf1N1B4_2880 0.45 -4.4
3,227,188 + +0.0
3,227,189 - Pf1N1B4_2880 0.45 +0.0
3,227,189 - Pf1N1B4_2880 0.45 +0.0
3,227,230 + Pf1N1B4_2880 0.49 +0.0
3,227,230 + Pf1N1B4_2880 0.49 -5.8
3,227,231 - Pf1N1B4_2880 0.49 -3.9
3,227,348 + Pf1N1B4_2880 0.61 -4.3
3,227,401 + Pf1N1B4_2880 0.66 +0.0
3,227,401 + Pf1N1B4_2880 0.66 +0.0
3,227,401 + Pf1N1B4_2880 0.66 -4.3
3,227,401 + Pf1N1B4_2880 0.66 -3.8
3,227,446 + Pf1N1B4_2880 0.70 -5.2
3,227,446 + Pf1N1B4_2880 0.70 -3.4
3,227,446 + Pf1N1B4_2880 0.70 -4.1
3,227,446 + Pf1N1B4_2880 0.70 -1.0
3,227,446 + Pf1N1B4_2880 0.70 -6.4
3,227,446 + Pf1N1B4_2880 0.70 +0.0
3,227,446 + Pf1N1B4_2880 0.70 -4.2
3,227,446 + Pf1N1B4_2880 0.70 +0.0
3,227,446 + Pf1N1B4_2880 0.70 -5.2
3,227,446 + Pf1N1B4_2880 0.70 +0.0
3,227,446 + Pf1N1B4_2880 0.70 -4.1
3,227,446 + Pf1N1B4_2880 0.70 -4.1
3,227,446 + Pf1N1B4_2880 0.70 -4.0
3,227,446 + Pf1N1B4_2880 0.70 -4.2
3,227,446 + Pf1N1B4_2880 0.70 +0.0
3,227,446 + Pf1N1B4_2880 0.70 -4.2
3,227,446 + Pf1N1B4_2880 0.70 +0.0
3,227,446 + Pf1N1B4_2880 0.70 +0.0
3,227,446 + Pf1N1B4_2880 0.70 -4.5
3,227,447 - Pf1N1B4_2880 0.70 +0.0
3,227,447 - Pf1N1B4_2880 0.70 -5.1
3,227,447 - Pf1N1B4_2880 0.70 +0.0
3,227,447 - Pf1N1B4_2880 0.70 -3.4
3,227,447 - Pf1N1B4_2880 0.70 +0.0
3,227,447 - Pf1N1B4_2880 0.70 -5.9
3,227,473 + Pf1N1B4_2880 0.73 -5.6
3,227,473 + Pf1N1B4_2880 0.73 +0.0
3,227,473 + Pf1N1B4_2880 0.73 -2.3
3,227,473 + Pf1N1B4_2880 0.73 -4.2
3,227,473 + Pf1N1B4_2880 0.73 -6.4
3,227,473 + Pf1N1B4_2880 0.73 +0.0
3,227,506 + Pf1N1B4_2880 0.76 -4.6
3,227,506 + Pf1N1B4_2880 0.76 +0.0
3,227,506 + Pf1N1B4_2880 0.76 -5.5
3,227,506 + Pf1N1B4_2880 0.76 +0.0
3,227,506 + Pf1N1B4_2880 0.76 -4.5
3,227,506 + Pf1N1B4_2880 0.76 +0.0
3,227,507 - Pf1N1B4_2880 0.76 -2.0
3,227,571 + Pf1N1B4_2880 0.82 -4.2
3,227,571 + Pf1N1B4_2880 0.82 -4.9
3,227,571 + Pf1N1B4_2880 0.82 +0.0
3,227,571 + Pf1N1B4_2880 0.82 +0.0
3,227,571 + Pf1N1B4_2880 0.82 +0.0
3,227,571 + Pf1N1B4_2880 0.82 -5.5
3,227,571 + Pf1N1B4_2880 0.82 +0.0
3,227,571 + Pf1N1B4_2880 0.82 +0.0
3,227,571 + Pf1N1B4_2880 0.82 -4.8
3,227,571 + Pf1N1B4_2880 0.82 +0.0
3,227,572 - Pf1N1B4_2880 0.83 +0.0
3,227,572 - Pf1N1B4_2880 0.83 +0.0
3,227,572 - Pf1N1B4_2880 0.83 +0.0
3,227,587 + Pf1N1B4_2880 0.84 +0.0
3,227,649 + -3.9
3,227,746 + +0.0
3,227,746 + +0.0
3,227,746 + +0.0
3,227,746 + -2.7
3,227,746 + -2.7
3,227,746 + +0.0
3,227,746 + +0.0
3,227,746 + -2.4
3,227,746 + +0.0
3,227,746 + -3.0
3,227,746 + +0.0
3,227,746 + +0.0
3,227,777 + +0.0
3,227,777 + +0.0
3,227,777 + +0.0
3,227,814 + +0.0
3,227,814 + -1.9
3,227,816 + +0.0
3,228,898 + -5.0
3,228,898 + +0.0
3,228,901 + +0.0
3,228,901 + +0.0
3,228,901 + -3.2
3,228,901 + +0.0
3,229,104 + Pf1N1B4_2882 0.28 -3.9
3,229,104 + Pf1N1B4_2882 0.28 -2.1
3,229,105 - Pf1N1B4_2882 0.29 -3.9
3,229,253 + -1.8
3,229,253 + Pf1N1B4_2882 0.43 +0.0
3,229,257 + Pf1N1B4_2882 0.43 -4.5
3,229,257 + Pf1N1B4_2882 0.43 +0.0
3,229,395 - Pf1N1B4_2882 0.57 +0.0
3,229,437 + Pf1N1B4_2882 0.61 -1.6
3,229,437 + Pf1N1B4_2882 0.61 +0.0
3,229,438 - Pf1N1B4_2882 0.61 +0.0
3,229,441 + Pf1N1B4_2882 0.62 -2.5
3,229,441 + Pf1N1B4_2882 0.62 +0.0
3,229,441 + Pf1N1B4_2882 0.62 -4.2
3,229,441 + Pf1N1B4_2882 0.62 -4.0
3,229,442 - -3.3
3,229,442 - Pf1N1B4_2882 0.62 +0.0
3,229,500 + Pf1N1B4_2882 0.67 +0.0
3,229,500 + Pf1N1B4_2882 0.67 -4.6
3,229,500 + Pf1N1B4_2882 0.67 -3.9
3,229,500 + Pf1N1B4_2882 0.67 +0.0
3,229,500 + Pf1N1B4_2882 0.67 -3.1
3,229,500 + Pf1N1B4_2882 0.67 +0.0
3,229,500 + Pf1N1B4_2882 0.67 +0.0
3,229,500 + Pf1N1B4_2882 0.67 +0.0
3,229,501 - Pf1N1B4_2882 0.68 -2.2
3,229,501 - Pf1N1B4_2882 0.68 +0.0
3,229,560 + Pf1N1B4_2882 0.73 +0.0
3,229,560 + Pf1N1B4_2882 0.73 -2.3
3,229,622 + Pf1N1B4_2882 0.79 -2.9
3,229,622 + Pf1N1B4_2882 0.79 +0.0
3,229,623 - Pf1N1B4_2882 0.80 -4.8
3,229,660 - Pf1N1B4_2882 0.83 +0.0

Or see this region's nucleotide sequence