Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2839

Experiment: Nitrate reduction, replicate C t3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2836 and Pf1N1B4_2837 are separated by 63 nucleotidesPf1N1B4_2837 and Pf1N1B4_2838 are separated by 9 nucleotidesPf1N1B4_2838 and Pf1N1B4_2839 overlap by 4 nucleotidesPf1N1B4_2839 and Pf1N1B4_2840 are separated by 8 nucleotidesPf1N1B4_2840 and Pf1N1B4_2841 are separated by 1 nucleotides Pf1N1B4_2836 - FIG028593: membrane protein, at 3,182,602 to 3,183,291 _2836 Pf1N1B4_2837 - FIG00956650: hypothetical protein, at 3,183,355 to 3,183,570 _2837 Pf1N1B4_2838 - FIG003276: zinc-binding protein, at 3,183,580 to 3,183,780 _2838 Pf1N1B4_2839 - Dephospho-CoA kinase (EC 2.7.1.24), at 3,183,777 to 3,184,388 _2839 Pf1N1B4_2840 - Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-), at 3,184,397 to 3,185,269 _2840 Pf1N1B4_2841 - Type IV fimbrial assembly protein PilC, at 3,185,271 to 3,186,488 _2841 Position (kb) 3183 3184 3185Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 3182.803 kb on + strand, within Pf1N1B4_2836at 3182.804 kb on - strand, within Pf1N1B4_2836at 3182.804 kb on - strand, within Pf1N1B4_2836at 3182.804 kb on - strand, within Pf1N1B4_2836at 3182.804 kb on - strand, within Pf1N1B4_2836at 3182.804 kb on - strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.019 kb on + strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strandat 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.020 kb on - strand, within Pf1N1B4_2836at 3183.021 kb on + strand, within Pf1N1B4_2836at 3183.021 kb on + strand, within Pf1N1B4_2836at 3183.021 kb on + strand, within Pf1N1B4_2836at 3183.021 kb on + strand, within Pf1N1B4_2836at 3183.021 kb on + strand, within Pf1N1B4_2836at 3183.021 kb on + strand, within Pf1N1B4_2836at 3183.021 kb on + strand, within Pf1N1B4_2836at 3183.021 kb on + strand, within Pf1N1B4_2836at 3183.097 kb on + strand, within Pf1N1B4_2836at 3183.097 kb on + strand, within Pf1N1B4_2836at 3183.098 kb on - strand, within Pf1N1B4_2836at 3183.145 kb on + strand, within Pf1N1B4_2836at 3183.155 kb on + strand, within Pf1N1B4_2836at 3183.178 kb on - strand, within Pf1N1B4_2836at 3183.364 kb on + strandat 3183.364 kb on + strandat 3183.364 kb on + strandat 3183.364 kb on + strandat 3183.364 kb on + strandat 3183.365 kb on - strandat 3183.365 kb on - strandat 3183.365 kb on - strandat 3183.489 kb on + strand, within Pf1N1B4_2837at 3183.489 kb on + strand, within Pf1N1B4_2837at 3183.489 kb on + strand, within Pf1N1B4_2837at 3183.489 kb on + strand, within Pf1N1B4_2837at 3183.489 kb on + strand, within Pf1N1B4_2837at 3183.490 kb on - strand, within Pf1N1B4_2837at 3183.490 kb on - strand, within Pf1N1B4_2837at 3183.491 kb on + strand, within Pf1N1B4_2837at 3183.491 kb on + strand, within Pf1N1B4_2837at 3183.491 kb on + strand, within Pf1N1B4_2837at 3183.491 kb on + strand, within Pf1N1B4_2837at 3183.491 kb on + strand, within Pf1N1B4_2837at 3183.491 kb on + strand, within Pf1N1B4_2837at 3183.491 kb on + strand, within Pf1N1B4_2837at 3183.491 kb on + strand, within Pf1N1B4_2837at 3183.491 kb on + strand, within Pf1N1B4_2837at 3183.491 kb on + strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strandat 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.492 kb on - strand, within Pf1N1B4_2837at 3183.550 kb on + strandat 3183.551 kb on - strandat 3183.558 kb on + strandat 3183.800 kb on + strandat 3183.800 kb on + strandat 3183.800 kb on + strandat 3183.800 kb on + strandat 3183.800 kb on + strandat 3183.801 kb on - strandat 3183.801 kb on - strandat 3183.801 kb on - strandat 3183.801 kb on - strandat 3183.801 kb on - strandat 3183.801 kb on - strandat 3183.801 kb on - strandat 3183.801 kb on - strandat 3183.801 kb on - strandat 3183.801 kb on - strandat 3184.395 kb on - strandat 3184.423 kb on - strandat 3184.423 kb on - strandat 3184.423 kb on - strandat 3184.423 kb on - strandat 3184.423 kb on - strandat 3184.434 kb on - strandat 3184.434 kb on - strandat 3184.480 kb on - strandat 3184.673 kb on + strand, within Pf1N1B4_2840at 3185.001 kb on + strand, within Pf1N1B4_2840at 3185.001 kb on + strand, within Pf1N1B4_2840at 3185.002 kb on - strandat 3185.002 kb on - strand, within Pf1N1B4_2840at 3185.002 kb on - strand, within Pf1N1B4_2840at 3185.002 kb on - strand, within Pf1N1B4_2840at 3185.002 kb on - strand, within Pf1N1B4_2840at 3185.073 kb on - strand, within Pf1N1B4_2840at 3185.082 kb on + strand, within Pf1N1B4_2840at 3185.082 kb on + strand, within Pf1N1B4_2840at 3185.082 kb on + strand, within Pf1N1B4_2840at 3185.083 kb on - strand, within Pf1N1B4_2840at 3185.083 kb on - strand, within Pf1N1B4_2840at 3185.083 kb on - strand, within Pf1N1B4_2840at 3185.083 kb on - strand, within Pf1N1B4_2840at 3185.083 kb on - strand, within Pf1N1B4_2840at 3185.269 kb on + strandat 3185.269 kb on + strandat 3185.270 kb on - strandat 3185.270 kb on - strandat 3185.270 kb on - strandat 3185.270 kb on - strandat 3185.270 kb on - strandat 3185.270 kb on - strandat 3185.305 kb on + strandat 3185.305 kb on + strandat 3185.306 kb on - strandat 3185.343 kb on + strandat 3185.344 kb on - strandat 3185.346 kb on + strandat 3185.346 kb on + strandat 3185.347 kb on - strandat 3185.347 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3
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3,182,803 + Pf1N1B4_2836 0.29 +0.0
3,182,804 - Pf1N1B4_2836 0.29 +0.0
3,182,804 - Pf1N1B4_2836 0.29 +0.0
3,182,804 - Pf1N1B4_2836 0.29 +0.0
3,182,804 - Pf1N1B4_2836 0.29 +0.0
3,182,804 - Pf1N1B4_2836 0.29 +0.0
3,183,019 + Pf1N1B4_2836 0.60 +1.6
3,183,019 + Pf1N1B4_2836 0.60 +0.0
3,183,019 + Pf1N1B4_2836 0.60 +0.0
3,183,019 + Pf1N1B4_2836 0.60 +0.0
3,183,019 + Pf1N1B4_2836 0.60 -4.2
3,183,019 + Pf1N1B4_2836 0.60 +0.0
3,183,019 + Pf1N1B4_2836 0.60 +0.0
3,183,019 + Pf1N1B4_2836 0.60 +0.0
3,183,019 + Pf1N1B4_2836 0.60 +0.0
3,183,019 + Pf1N1B4_2836 0.60 +0.0
3,183,019 + Pf1N1B4_2836 0.60 +0.0
3,183,019 + Pf1N1B4_2836 0.60 +0.0
3,183,020 - Pf1N1B4_2836 0.61 -1.6
3,183,020 - Pf1N1B4_2836 0.61 -2.3
3,183,020 - Pf1N1B4_2836 0.61 -2.9
3,183,020 - Pf1N1B4_2836 0.61 +0.0
3,183,020 - Pf1N1B4_2836 0.61 +0.0
3,183,020 - +0.0
3,183,020 - Pf1N1B4_2836 0.61 +0.0
3,183,020 - Pf1N1B4_2836 0.61 +0.0
3,183,020 - Pf1N1B4_2836 0.61 -2.3
3,183,020 - Pf1N1B4_2836 0.61 +0.0
3,183,020 - Pf1N1B4_2836 0.61 -1.9
3,183,020 - Pf1N1B4_2836 0.61 +0.0
3,183,020 - Pf1N1B4_2836 0.61 +0.0
3,183,020 - Pf1N1B4_2836 0.61 +0.0
3,183,020 - Pf1N1B4_2836 0.61 +0.0
3,183,021 + Pf1N1B4_2836 0.61 -1.2
3,183,021 + Pf1N1B4_2836 0.61 +0.0
3,183,021 + Pf1N1B4_2836 0.61 +0.0
3,183,021 + Pf1N1B4_2836 0.61 -1.8
3,183,021 + Pf1N1B4_2836 0.61 +0.0
3,183,021 + Pf1N1B4_2836 0.61 +0.0
3,183,021 + Pf1N1B4_2836 0.61 +0.0
3,183,021 + Pf1N1B4_2836 0.61 +0.0
3,183,097 + Pf1N1B4_2836 0.72 +0.0
3,183,097 + Pf1N1B4_2836 0.72 -2.3
3,183,098 - Pf1N1B4_2836 0.72 +0.0
3,183,145 + Pf1N1B4_2836 0.79 +0.0
3,183,155 + Pf1N1B4_2836 0.80 +0.0
3,183,178 - Pf1N1B4_2836 0.83 -2.6
3,183,364 + +0.0
3,183,364 + -0.5
3,183,364 + +0.0
3,183,364 + +0.0
3,183,364 + -0.4
3,183,365 - -2.3
3,183,365 - -2.4
3,183,365 - +0.0
3,183,489 + Pf1N1B4_2837 0.62 -0.4
3,183,489 + Pf1N1B4_2837 0.62 +0.0
3,183,489 + Pf1N1B4_2837 0.62 +1.8
3,183,489 + Pf1N1B4_2837 0.62 -1.2
3,183,489 + Pf1N1B4_2837 0.62 -1.6
3,183,490 - Pf1N1B4_2837 0.62 -1.0
3,183,490 - Pf1N1B4_2837 0.62 -1.0
3,183,491 + Pf1N1B4_2837 0.63 +0.0
3,183,491 + Pf1N1B4_2837 0.63 +0.0
3,183,491 + Pf1N1B4_2837 0.63 -0.5
3,183,491 + Pf1N1B4_2837 0.63 +0.0
3,183,491 + Pf1N1B4_2837 0.63 +0.0
3,183,491 + Pf1N1B4_2837 0.63 -1.2
3,183,491 + Pf1N1B4_2837 0.63 +0.7
3,183,491 + Pf1N1B4_2837 0.63 +0.0
3,183,491 + Pf1N1B4_2837 0.63 +0.2
3,183,491 + Pf1N1B4_2837 0.63 +0.1
3,183,492 - Pf1N1B4_2837 0.63 +1.0
3,183,492 - Pf1N1B4_2837 0.63 +0.0
3,183,492 - Pf1N1B4_2837 0.63 -2.8
3,183,492 - Pf1N1B4_2837 0.63 +0.0
3,183,492 - Pf1N1B4_2837 0.63 -0.8
3,183,492 - Pf1N1B4_2837 0.63 +0.4
3,183,492 - Pf1N1B4_2837 0.63 +0.0
3,183,492 - Pf1N1B4_2837 0.63 +0.0
3,183,492 - Pf1N1B4_2837 0.63 +0.0
3,183,492 - Pf1N1B4_2837 0.63 +0.0
3,183,492 - +1.4
3,183,492 - Pf1N1B4_2837 0.63 +0.0
3,183,492 - Pf1N1B4_2837 0.63 +0.0
3,183,492 - Pf1N1B4_2837 0.63 +0.0
3,183,492 - Pf1N1B4_2837 0.63 +0.0
3,183,492 - Pf1N1B4_2837 0.63 +0.0
3,183,550 + -2.9
3,183,551 - -2.1
3,183,558 + +0.0
3,183,800 + +0.0
3,183,800 + -1.8
3,183,800 + +0.0
3,183,800 + -2.7
3,183,800 + +0.0
3,183,801 - +0.0
3,183,801 - +0.0
3,183,801 - +0.0
3,183,801 - +0.0
3,183,801 - +0.0
3,183,801 - -4.4
3,183,801 - +0.0
3,183,801 - +0.0
3,183,801 - +0.0
3,183,801 - +0.0
3,184,395 - +0.0
3,184,423 - -1.8
3,184,423 - +0.0
3,184,423 - -1.9
3,184,423 - -2.8
3,184,423 - +0.0
3,184,434 - -1.9
3,184,434 - +0.0
3,184,480 - +0.0
3,184,673 + Pf1N1B4_2840 0.32 +0.0
3,185,001 + Pf1N1B4_2840 0.69 +0.0
3,185,001 + Pf1N1B4_2840 0.69 -4.7
3,185,002 - -1.5
3,185,002 - Pf1N1B4_2840 0.69 +0.0
3,185,002 - Pf1N1B4_2840 0.69 +0.0
3,185,002 - Pf1N1B4_2840 0.69 +0.0
3,185,002 - Pf1N1B4_2840 0.69 -4.9
3,185,073 - Pf1N1B4_2840 0.77 -5.2
3,185,082 + Pf1N1B4_2840 0.78 -3.4
3,185,082 + Pf1N1B4_2840 0.78 -3.9
3,185,082 + Pf1N1B4_2840 0.78 +0.0
3,185,083 - Pf1N1B4_2840 0.79 +0.0
3,185,083 - Pf1N1B4_2840 0.79 +0.0
3,185,083 - Pf1N1B4_2840 0.79 +0.0
3,185,083 - Pf1N1B4_2840 0.79 +0.0
3,185,083 - Pf1N1B4_2840 0.79 +0.0
3,185,269 + +0.0
3,185,269 + +0.0
3,185,270 - +0.0
3,185,270 - +0.0
3,185,270 - -1.6
3,185,270 - +0.0
3,185,270 - +0.0
3,185,270 - -2.4
3,185,305 + +0.0
3,185,305 + +0.0
3,185,306 - +0.0
3,185,343 + -3.7
3,185,344 - -4.7
3,185,346 + -3.7
3,185,346 + +0.0
3,185,347 - -1.2
3,185,347 - -5.0

Or see this region's nucleotide sequence