Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2740 and Pf1N1B4_2741 overlap by 4 nucleotides Pf1N1B4_2741 and Pf1N1B4_2742 are separated by 141 nucleotides Pf1N1B4_2742 and Pf1N1B4_2743 overlap by 1 nucleotides
Pf1N1B4_2740 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 3,078,941 to 3,079,810
_2740
Pf1N1B4_2741 - Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family, at 3,079,807 to 3,080,400
_2741
Pf1N1B4_2742 - putative exported protein, at 3,080,542 to 3,081,501
_2742
Pf1N1B4_2743 - YpfJ protein, zinc metalloprotease superfamily, at 3,081,501 to 3,082,394
_2743
Position (kb)
3080
3081
3082 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3079.680 kb on - strand, within Pf1N1B4_2740 at 3080.118 kb on - strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.160 kb on - strand, within Pf1N1B4_2741 at 3080.160 kb on - strand, within Pf1N1B4_2741 at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.478 kb on + strand at 3080.580 kb on + strand at 3080.581 kb on - strand at 3080.727 kb on + strand, within Pf1N1B4_2742 at 3080.770 kb on + strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.834 kb on + strand, within Pf1N1B4_2742 at 3080.834 kb on + strand, within Pf1N1B4_2742 at 3080.835 kb on - strand, within Pf1N1B4_2742 at 3080.859 kb on - strand, within Pf1N1B4_2742 at 3080.859 kb on - strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.955 kb on - strand, within Pf1N1B4_2742 at 3080.955 kb on - strand, within Pf1N1B4_2742 at 3081.136 kb on - strand, within Pf1N1B4_2742 at 3081.188 kb on - strand, within Pf1N1B4_2742 at 3081.188 kb on - strand, within Pf1N1B4_2742 at 3081.308 kb on + strand, within Pf1N1B4_2742 at 3081.309 kb on - strand at 3081.475 kb on + strand at 3081.495 kb on + strand at 3081.495 kb on + strand at 3081.495 kb on + strand at 3081.495 kb on + strand at 3081.496 kb on - strand at 3081.496 kb on - strand at 3081.496 kb on - strand at 3081.496 kb on - strand at 3081.882 kb on + strand, within Pf1N1B4_2743 at 3081.883 kb on - strand, within Pf1N1B4_2743 at 3081.883 kb on - strand, within Pf1N1B4_2743 at 3081.883 kb on - strand, within Pf1N1B4_2743 at 3081.883 kb on - strand, within Pf1N1B4_2743 at 3081.883 kb on - strand, within Pf1N1B4_2743 at 3081.886 kb on + strand, within Pf1N1B4_2743 at 3081.887 kb on - strand, within Pf1N1B4_2743 at 3081.887 kb on - strand at 3081.887 kb on - strand, within Pf1N1B4_2743 at 3081.955 kb on + strand, within Pf1N1B4_2743 at 3081.955 kb on + strand, within Pf1N1B4_2743 at 3081.955 kb on + strand, within Pf1N1B4_2743 at 3081.955 kb on + strand, within Pf1N1B4_2743 at 3081.955 kb on + strand, within Pf1N1B4_2743 at 3081.956 kb on - strand, within Pf1N1B4_2743 at 3081.956 kb on - strand, within Pf1N1B4_2743 at 3081.956 kb on - strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.980 kb on - strand, within Pf1N1B4_2743 at 3082.058 kb on + strand, within Pf1N1B4_2743 at 3082.058 kb on + strand, within Pf1N1B4_2743 at 3082.058 kb on + strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.061 kb on - strand, within Pf1N1B4_2743 at 3082.061 kb on - strand, within Pf1N1B4_2743 at 3082.451 kb on + strand at 3082.451 kb on + strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.455 kb on + strand at 3082.456 kb on - strand at 3082.456 kb on - strand at 3082.456 kb on - strand at 3082.456 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 3,079,680 - Pf1N1B4_2740 0.85 -0.0 3,080,118 - Pf1N1B4_2741 0.52 -0.0 3,080,159 + Pf1N1B4_2741 0.59 -5.5 3,080,159 + Pf1N1B4_2741 0.59 -2.7 3,080,159 + Pf1N1B4_2741 0.59 -2.3 3,080,159 + Pf1N1B4_2741 0.59 -0.0 3,080,160 - Pf1N1B4_2741 0.59 -0.0 3,080,160 - Pf1N1B4_2741 0.59 -3.2 3,080,405 + -0.0 3,080,405 + -0.0 3,080,405 + -2.4 3,080,405 + -0.0 3,080,405 + -0.0 3,080,405 + -0.0 3,080,405 + -3.1 3,080,405 + -2.9 3,080,405 + -2.1 3,080,406 - -0.0 3,080,406 - -0.0 3,080,406 - -2.3 3,080,406 - -0.0 3,080,406 - -0.0 3,080,406 - -0.0 3,080,478 + -0.0 3,080,580 + -1.7 3,080,581 - -2.2 3,080,727 + Pf1N1B4_2742 0.19 -0.0 3,080,770 + Pf1N1B4_2742 0.24 -0.0 3,080,771 - Pf1N1B4_2742 0.24 -1.8 3,080,771 - Pf1N1B4_2742 0.24 -3.9 3,080,771 - Pf1N1B4_2742 0.24 -0.0 3,080,771 - Pf1N1B4_2742 0.24 -0.0 3,080,774 + Pf1N1B4_2742 0.24 -0.0 3,080,774 + Pf1N1B4_2742 0.24 -0.0 3,080,774 + Pf1N1B4_2742 0.24 -0.7 3,080,774 + Pf1N1B4_2742 0.24 -0.0 3,080,775 - Pf1N1B4_2742 0.24 -3.7 3,080,775 - Pf1N1B4_2742 0.24 -2.5 3,080,775 - Pf1N1B4_2742 0.24 -0.0 3,080,775 - Pf1N1B4_2742 0.24 -3.2 3,080,795 + Pf1N1B4_2742 0.26 -0.0 3,080,795 + Pf1N1B4_2742 0.26 -1.9 3,080,795 + Pf1N1B4_2742 0.26 -0.0 3,080,834 + Pf1N1B4_2742 0.30 -0.0 3,080,834 + Pf1N1B4_2742 0.30 -0.6 3,080,835 - Pf1N1B4_2742 0.31 -0.0 3,080,859 - Pf1N1B4_2742 0.33 -0.0 3,080,859 - Pf1N1B4_2742 0.33 -2.7 3,080,924 + Pf1N1B4_2742 0.40 -0.0 3,080,924 + Pf1N1B4_2742 0.40 -0.0 3,080,924 + Pf1N1B4_2742 0.40 -0.0 3,080,924 + Pf1N1B4_2742 0.40 -0.0 3,080,924 + Pf1N1B4_2742 0.40 -2.4 3,080,924 + Pf1N1B4_2742 0.40 -0.0 3,080,924 + Pf1N1B4_2742 0.40 -2.5 3,080,925 - Pf1N1B4_2742 0.40 -1.7 3,080,925 - Pf1N1B4_2742 0.40 -0.0 3,080,925 - Pf1N1B4_2742 0.40 -3.1 3,080,925 - Pf1N1B4_2742 0.40 -0.0 3,080,925 - Pf1N1B4_2742 0.40 -0.0 3,080,930 + Pf1N1B4_2742 0.40 -0.0 3,080,930 + Pf1N1B4_2742 0.40 -0.0 3,080,930 + Pf1N1B4_2742 0.40 -3.7 3,080,931 - Pf1N1B4_2742 0.41 -0.0 3,080,931 - Pf1N1B4_2742 0.41 -0.0 3,080,931 - Pf1N1B4_2742 0.41 -0.0 3,080,954 + Pf1N1B4_2742 0.43 -0.0 3,080,954 + Pf1N1B4_2742 0.43 -0.0 3,080,954 + Pf1N1B4_2742 0.43 -0.0 3,080,955 - Pf1N1B4_2742 0.43 -0.0 3,080,955 - Pf1N1B4_2742 0.43 -0.0 3,081,136 - Pf1N1B4_2742 0.62 -0.0 3,081,188 - Pf1N1B4_2742 0.67 -0.0 3,081,188 - Pf1N1B4_2742 0.67 -3.8 3,081,308 + Pf1N1B4_2742 0.80 -0.0 3,081,309 - -1.1 3,081,475 + -4.5 3,081,495 + -0.0 3,081,495 + -0.0 3,081,495 + -0.0 3,081,495 + -0.0 3,081,496 - -0.0 3,081,496 - -0.0 3,081,496 - -2.0 3,081,496 - -0.0 3,081,882 + Pf1N1B4_2743 0.43 -0.0 3,081,883 - Pf1N1B4_2743 0.43 -0.8 3,081,883 - Pf1N1B4_2743 0.43 -1.8 3,081,883 - Pf1N1B4_2743 0.43 -0.0 3,081,883 - Pf1N1B4_2743 0.43 -0.0 3,081,883 - Pf1N1B4_2743 0.43 -0.0 3,081,886 + Pf1N1B4_2743 0.43 -0.0 3,081,887 - Pf1N1B4_2743 0.43 -0.0 3,081,887 - -1.0 3,081,887 - Pf1N1B4_2743 0.43 -0.0 3,081,955 + Pf1N1B4_2743 0.51 -0.0 3,081,955 + Pf1N1B4_2743 0.51 -0.0 3,081,955 + Pf1N1B4_2743 0.51 -0.0 3,081,955 + Pf1N1B4_2743 0.51 -0.0 3,081,955 + Pf1N1B4_2743 0.51 -0.0 3,081,956 - Pf1N1B4_2743 0.51 -0.0 3,081,956 - Pf1N1B4_2743 0.51 -0.0 3,081,956 - Pf1N1B4_2743 0.51 -0.0 3,081,979 + Pf1N1B4_2743 0.53 -0.0 3,081,979 + Pf1N1B4_2743 0.53 -5.6 3,081,979 + Pf1N1B4_2743 0.53 -1.2 3,081,979 + Pf1N1B4_2743 0.53 -0.0 3,081,979 + Pf1N1B4_2743 0.53 -0.0 3,081,979 + Pf1N1B4_2743 0.53 -0.0 3,081,980 - Pf1N1B4_2743 0.54 -2.5 3,082,058 + Pf1N1B4_2743 0.62 -0.0 3,082,058 + Pf1N1B4_2743 0.62 -0.0 3,082,058 + Pf1N1B4_2743 0.62 -0.0 3,082,059 - Pf1N1B4_2743 0.62 -0.0 3,082,059 - Pf1N1B4_2743 0.62 -0.0 3,082,059 - Pf1N1B4_2743 0.62 -1.6 3,082,059 - Pf1N1B4_2743 0.62 -0.0 3,082,059 - Pf1N1B4_2743 0.62 -1.0 3,082,059 - Pf1N1B4_2743 0.62 -0.0 3,082,059 - Pf1N1B4_2743 0.62 -0.0 3,082,059 - Pf1N1B4_2743 0.62 -0.0 3,082,059 - Pf1N1B4_2743 0.62 -1.9 3,082,059 - Pf1N1B4_2743 0.62 -0.0 3,082,059 - Pf1N1B4_2743 0.62 -0.0 3,082,059 - Pf1N1B4_2743 0.62 -2.3 3,082,061 - Pf1N1B4_2743 0.63 -0.0 3,082,061 - Pf1N1B4_2743 0.63 -0.0 3,082,451 + -2.5 3,082,451 + -2.6 3,082,452 - -0.0 3,082,452 - -1.8 3,082,452 - -2.5 3,082,452 - -0.0 3,082,452 - -0.0 3,082,452 - -0.0 3,082,452 - -0.0 3,082,452 - -0.0 3,082,455 + -0.0 3,082,456 - -0.0 3,082,456 - -2.2 3,082,456 - -0.0 3,082,456 - -0.0
Or see this region's nucleotide sequence