Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2657 and Pf1N1B4_2658 overlap by 4 nucleotides Pf1N1B4_2658 and Pf1N1B4_2659 overlap by 1 nucleotides Pf1N1B4_2659 and Pf1N1B4_2660 are separated by 108 nucleotides
Pf1N1B4_2657 - GTP cyclohydrolase II (EC 3.5.4.25), at 2,993,243 to 2,993,860
_2657
Pf1N1B4_2658 - Permeases of the major facilitator superfamily, at 2,993,857 to 2,994,276
_2658
Pf1N1B4_2659 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 2,994,276 to 2,995,076
_2659
Pf1N1B4_2660 - Outer membrane vitamin B12 receptor BtuB, at 2,995,185 to 2,997,068
_2660
Position (kb)
2994
2995
2996 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2993.905 kb on + strand, within Pf1N1B4_2658 at 2993.906 kb on - strand, within Pf1N1B4_2658 at 2994.244 kb on + strand at 2994.244 kb on + strand at 2994.244 kb on + strand at 2994.244 kb on + strand at 2994.244 kb on + strand at 2994.244 kb on + strand at 2994.245 kb on - strand at 2994.245 kb on - strand at 2994.245 kb on - strand at 2994.245 kb on - strand at 2994.245 kb on - strand at 2994.256 kb on - strand at 2994.468 kb on + strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.739 kb on + strand, within Pf1N1B4_2659 at 2994.740 kb on - strand, within Pf1N1B4_2659 at 2994.750 kb on + strand, within Pf1N1B4_2659 at 2994.751 kb on - strand, within Pf1N1B4_2659 at 2994.774 kb on + strand, within Pf1N1B4_2659 at 2994.774 kb on + strand, within Pf1N1B4_2659 at 2994.775 kb on - strand, within Pf1N1B4_2659 at 2995.267 kb on + strand at 2995.267 kb on + strand at 2995.268 kb on - strand at 2995.268 kb on - strand at 2995.370 kb on - strand at 2995.405 kb on + strand, within Pf1N1B4_2660 at 2995.405 kb on + strand at 2995.406 kb on - strand, within Pf1N1B4_2660 at 2995.732 kb on + strand, within Pf1N1B4_2660 at 2995.732 kb on + strand, within Pf1N1B4_2660 at 2995.733 kb on - strand, within Pf1N1B4_2660 at 2995.733 kb on - strand, within Pf1N1B4_2660 at 2995.733 kb on - strand, within Pf1N1B4_2660 at 2995.733 kb on - strand, within Pf1N1B4_2660 at 2995.733 kb on - strand, within Pf1N1B4_2660 at 2995.733 kb on - strand, within Pf1N1B4_2660 at 2995.733 kb on - strand, within Pf1N1B4_2660 at 2995.780 kb on + strand, within Pf1N1B4_2660 at 2995.783 kb on + strand, within Pf1N1B4_2660 at 2995.784 kb on - strand, within Pf1N1B4_2660 at 2995.784 kb on - strand, within Pf1N1B4_2660 at 2995.784 kb on - strand, within Pf1N1B4_2660 at 2995.784 kb on - strand, within Pf1N1B4_2660 at 2995.784 kb on - strand, within Pf1N1B4_2660 at 2995.822 kb on + strand, within Pf1N1B4_2660 at 2995.822 kb on + strand, within Pf1N1B4_2660 at 2995.822 kb on + strand, within Pf1N1B4_2660 at 2995.822 kb on + strand, within Pf1N1B4_2660 at 2995.823 kb on - strand, within Pf1N1B4_2660 at 2995.823 kb on - strand, within Pf1N1B4_2660 at 2996.029 kb on + strand, within Pf1N1B4_2660
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 2,993,905 + Pf1N1B4_2658 0.11 -0.0 2,993,906 - Pf1N1B4_2658 0.12 -4.4 2,994,244 + -0.0 2,994,244 + -0.0 2,994,244 + -0.0 2,994,244 + -2.8 2,994,244 + -0.0 2,994,244 + -0.0 2,994,245 - -0.0 2,994,245 - -3.8 2,994,245 - -2.6 2,994,245 - -0.0 2,994,245 - -0.0 2,994,256 - -0.0 2,994,468 + Pf1N1B4_2659 0.24 -0.0 2,994,469 - Pf1N1B4_2659 0.24 -0.0 2,994,469 - Pf1N1B4_2659 0.24 -3.2 2,994,469 - Pf1N1B4_2659 0.24 -0.0 2,994,469 - Pf1N1B4_2659 0.24 -3.3 2,994,469 - Pf1N1B4_2659 0.24 -0.0 2,994,469 - Pf1N1B4_2659 0.24 -3.5 2,994,469 - Pf1N1B4_2659 0.24 -0.0 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,585 + Pf1N1B4_2659 0.39 -5.0 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,585 + -2.2 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,585 + Pf1N1B4_2659 0.39 -4.1 2,994,585 + Pf1N1B4_2659 0.39 -2.5 2,994,585 + -3.3 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,585 + Pf1N1B4_2659 0.39 -0.0 2,994,586 - Pf1N1B4_2659 0.39 -0.0 2,994,586 - Pf1N1B4_2659 0.39 -3.2 2,994,586 - Pf1N1B4_2659 0.39 -0.0 2,994,586 - Pf1N1B4_2659 0.39 -3.1 2,994,586 - Pf1N1B4_2659 0.39 -5.4 2,994,586 - Pf1N1B4_2659 0.39 -3.3 2,994,586 - Pf1N1B4_2659 0.39 -3.8 2,994,586 - Pf1N1B4_2659 0.39 -0.0 2,994,586 - -0.0 2,994,586 - Pf1N1B4_2659 0.39 -3.9 2,994,586 - Pf1N1B4_2659 0.39 -0.0 2,994,586 - Pf1N1B4_2659 0.39 -0.0 2,994,586 - Pf1N1B4_2659 0.39 -4.0 2,994,586 - Pf1N1B4_2659 0.39 -3.7 2,994,586 - Pf1N1B4_2659 0.39 -5.5 2,994,739 + Pf1N1B4_2659 0.58 -0.0 2,994,740 - Pf1N1B4_2659 0.58 -0.0 2,994,750 + Pf1N1B4_2659 0.59 -0.0 2,994,751 - Pf1N1B4_2659 0.59 -0.0 2,994,774 + Pf1N1B4_2659 0.62 -0.0 2,994,774 + Pf1N1B4_2659 0.62 -0.0 2,994,775 - Pf1N1B4_2659 0.62 -0.0 2,995,267 + -4.2 2,995,267 + -1.8 2,995,268 - -3.6 2,995,268 - -2.8 2,995,370 - -4.8 2,995,405 + Pf1N1B4_2660 0.12 -0.0 2,995,405 + -0.0 2,995,406 - Pf1N1B4_2660 0.12 -2.9 2,995,732 + Pf1N1B4_2660 0.29 -3.0 2,995,732 + Pf1N1B4_2660 0.29 -0.0 2,995,733 - Pf1N1B4_2660 0.29 -2.2 2,995,733 - Pf1N1B4_2660 0.29 -0.0 2,995,733 - Pf1N1B4_2660 0.29 -0.0 2,995,733 - Pf1N1B4_2660 0.29 -0.0 2,995,733 - Pf1N1B4_2660 0.29 -5.6 2,995,733 - Pf1N1B4_2660 0.29 -3.2 2,995,733 - Pf1N1B4_2660 0.29 -0.0 2,995,780 + Pf1N1B4_2660 0.32 -0.0 2,995,783 + Pf1N1B4_2660 0.32 -0.0 2,995,784 - Pf1N1B4_2660 0.32 -4.0 2,995,784 - Pf1N1B4_2660 0.32 -0.0 2,995,784 - Pf1N1B4_2660 0.32 -4.1 2,995,784 - Pf1N1B4_2660 0.32 -0.0 2,995,784 - Pf1N1B4_2660 0.32 -4.5 2,995,822 + Pf1N1B4_2660 0.34 -2.9 2,995,822 + Pf1N1B4_2660 0.34 -0.0 2,995,822 + Pf1N1B4_2660 0.34 -2.2 2,995,822 + Pf1N1B4_2660 0.34 -0.0 2,995,823 - Pf1N1B4_2660 0.34 -0.0 2,995,823 - Pf1N1B4_2660 0.34 -0.0 2,996,029 + Pf1N1B4_2660 0.45 -0.0
Or see this region's nucleotide sequence