Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2659

Experiment: Nitrate reduction, replicate C t3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2657 and Pf1N1B4_2658 overlap by 4 nucleotidesPf1N1B4_2658 and Pf1N1B4_2659 overlap by 1 nucleotidesPf1N1B4_2659 and Pf1N1B4_2660 are separated by 108 nucleotides Pf1N1B4_2657 - GTP cyclohydrolase II (EC 3.5.4.25), at 2,993,243 to 2,993,860 _2657 Pf1N1B4_2658 - Permeases of the major facilitator superfamily, at 2,993,857 to 2,994,276 _2658 Pf1N1B4_2659 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 2,994,276 to 2,995,076 _2659 Pf1N1B4_2660 - Outer membrane vitamin B12 receptor BtuB, at 2,995,185 to 2,997,068 _2660 Position (kb) 2994 2995 2996Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2993.905 kb on + strand, within Pf1N1B4_2658at 2993.906 kb on - strand, within Pf1N1B4_2658at 2994.244 kb on + strandat 2994.244 kb on + strandat 2994.244 kb on + strandat 2994.244 kb on + strandat 2994.244 kb on + strandat 2994.244 kb on + strandat 2994.245 kb on - strandat 2994.245 kb on - strandat 2994.245 kb on - strandat 2994.245 kb on - strandat 2994.245 kb on - strandat 2994.256 kb on - strandat 2994.468 kb on + strand, within Pf1N1B4_2659at 2994.469 kb on - strand, within Pf1N1B4_2659at 2994.469 kb on - strand, within Pf1N1B4_2659at 2994.469 kb on - strand, within Pf1N1B4_2659at 2994.469 kb on - strand, within Pf1N1B4_2659at 2994.469 kb on - strand, within Pf1N1B4_2659at 2994.469 kb on - strand, within Pf1N1B4_2659at 2994.469 kb on - strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strandat 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strandat 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.585 kb on + strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strandat 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.586 kb on - strand, within Pf1N1B4_2659at 2994.739 kb on + strand, within Pf1N1B4_2659at 2994.740 kb on - strand, within Pf1N1B4_2659at 2994.750 kb on + strand, within Pf1N1B4_2659at 2994.751 kb on - strand, within Pf1N1B4_2659at 2994.774 kb on + strand, within Pf1N1B4_2659at 2994.774 kb on + strand, within Pf1N1B4_2659at 2994.775 kb on - strand, within Pf1N1B4_2659at 2995.267 kb on + strandat 2995.267 kb on + strandat 2995.268 kb on - strandat 2995.268 kb on - strandat 2995.370 kb on - strandat 2995.405 kb on + strand, within Pf1N1B4_2660at 2995.405 kb on + strandat 2995.406 kb on - strand, within Pf1N1B4_2660at 2995.732 kb on + strand, within Pf1N1B4_2660at 2995.732 kb on + strand, within Pf1N1B4_2660at 2995.733 kb on - strand, within Pf1N1B4_2660at 2995.733 kb on - strand, within Pf1N1B4_2660at 2995.733 kb on - strand, within Pf1N1B4_2660at 2995.733 kb on - strand, within Pf1N1B4_2660at 2995.733 kb on - strand, within Pf1N1B4_2660at 2995.733 kb on - strand, within Pf1N1B4_2660at 2995.733 kb on - strand, within Pf1N1B4_2660at 2995.780 kb on + strand, within Pf1N1B4_2660at 2995.783 kb on + strand, within Pf1N1B4_2660at 2995.784 kb on - strand, within Pf1N1B4_2660at 2995.784 kb on - strand, within Pf1N1B4_2660at 2995.784 kb on - strand, within Pf1N1B4_2660at 2995.784 kb on - strand, within Pf1N1B4_2660at 2995.784 kb on - strand, within Pf1N1B4_2660at 2995.822 kb on + strand, within Pf1N1B4_2660at 2995.822 kb on + strand, within Pf1N1B4_2660at 2995.822 kb on + strand, within Pf1N1B4_2660at 2995.822 kb on + strand, within Pf1N1B4_2660at 2995.823 kb on - strand, within Pf1N1B4_2660at 2995.823 kb on - strand, within Pf1N1B4_2660at 2996.029 kb on + strand, within Pf1N1B4_2660

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3
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2,993,905 + Pf1N1B4_2658 0.11 -0.0
2,993,906 - Pf1N1B4_2658 0.12 -4.4
2,994,244 + -0.0
2,994,244 + -0.0
2,994,244 + -0.0
2,994,244 + -2.8
2,994,244 + -0.0
2,994,244 + -0.0
2,994,245 - -0.0
2,994,245 - -3.8
2,994,245 - -2.6
2,994,245 - -0.0
2,994,245 - -0.0
2,994,256 - -0.0
2,994,468 + Pf1N1B4_2659 0.24 -0.0
2,994,469 - Pf1N1B4_2659 0.24 -0.0
2,994,469 - Pf1N1B4_2659 0.24 -3.2
2,994,469 - Pf1N1B4_2659 0.24 -0.0
2,994,469 - Pf1N1B4_2659 0.24 -3.3
2,994,469 - Pf1N1B4_2659 0.24 -0.0
2,994,469 - Pf1N1B4_2659 0.24 -3.5
2,994,469 - Pf1N1B4_2659 0.24 -0.0
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,585 + Pf1N1B4_2659 0.39 -5.0
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,585 + -2.2
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,585 + Pf1N1B4_2659 0.39 -4.1
2,994,585 + Pf1N1B4_2659 0.39 -2.5
2,994,585 + -3.3
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,585 + Pf1N1B4_2659 0.39 -0.0
2,994,586 - Pf1N1B4_2659 0.39 -0.0
2,994,586 - Pf1N1B4_2659 0.39 -3.2
2,994,586 - Pf1N1B4_2659 0.39 -0.0
2,994,586 - Pf1N1B4_2659 0.39 -3.1
2,994,586 - Pf1N1B4_2659 0.39 -5.4
2,994,586 - Pf1N1B4_2659 0.39 -3.3
2,994,586 - Pf1N1B4_2659 0.39 -3.8
2,994,586 - Pf1N1B4_2659 0.39 -0.0
2,994,586 - -0.0
2,994,586 - Pf1N1B4_2659 0.39 -3.9
2,994,586 - Pf1N1B4_2659 0.39 -0.0
2,994,586 - Pf1N1B4_2659 0.39 -0.0
2,994,586 - Pf1N1B4_2659 0.39 -4.0
2,994,586 - Pf1N1B4_2659 0.39 -3.7
2,994,586 - Pf1N1B4_2659 0.39 -5.5
2,994,739 + Pf1N1B4_2659 0.58 -0.0
2,994,740 - Pf1N1B4_2659 0.58 -0.0
2,994,750 + Pf1N1B4_2659 0.59 -0.0
2,994,751 - Pf1N1B4_2659 0.59 -0.0
2,994,774 + Pf1N1B4_2659 0.62 -0.0
2,994,774 + Pf1N1B4_2659 0.62 -0.0
2,994,775 - Pf1N1B4_2659 0.62 -0.0
2,995,267 + -4.2
2,995,267 + -1.8
2,995,268 - -3.6
2,995,268 - -2.8
2,995,370 - -4.8
2,995,405 + Pf1N1B4_2660 0.12 -0.0
2,995,405 + -0.0
2,995,406 - Pf1N1B4_2660 0.12 -2.9
2,995,732 + Pf1N1B4_2660 0.29 -3.0
2,995,732 + Pf1N1B4_2660 0.29 -0.0
2,995,733 - Pf1N1B4_2660 0.29 -2.2
2,995,733 - Pf1N1B4_2660 0.29 -0.0
2,995,733 - Pf1N1B4_2660 0.29 -0.0
2,995,733 - Pf1N1B4_2660 0.29 -0.0
2,995,733 - Pf1N1B4_2660 0.29 -5.6
2,995,733 - Pf1N1B4_2660 0.29 -3.2
2,995,733 - Pf1N1B4_2660 0.29 -0.0
2,995,780 + Pf1N1B4_2660 0.32 -0.0
2,995,783 + Pf1N1B4_2660 0.32 -0.0
2,995,784 - Pf1N1B4_2660 0.32 -4.0
2,995,784 - Pf1N1B4_2660 0.32 -0.0
2,995,784 - Pf1N1B4_2660 0.32 -4.1
2,995,784 - Pf1N1B4_2660 0.32 -0.0
2,995,784 - Pf1N1B4_2660 0.32 -4.5
2,995,822 + Pf1N1B4_2660 0.34 -2.9
2,995,822 + Pf1N1B4_2660 0.34 -0.0
2,995,822 + Pf1N1B4_2660 0.34 -2.2
2,995,822 + Pf1N1B4_2660 0.34 -0.0
2,995,823 - Pf1N1B4_2660 0.34 -0.0
2,995,823 - Pf1N1B4_2660 0.34 -0.0
2,996,029 + Pf1N1B4_2660 0.45 -0.0

Or see this region's nucleotide sequence