Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2555

Experiment: Nitrate reduction, replicate C t3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2553 and Pf1N1B4_2554 are separated by 184 nucleotidesPf1N1B4_2554 and Pf1N1B4_2555 are separated by 82 nucleotidesPf1N1B4_2555 and Pf1N1B4_2556 are separated by 50 nucleotides Pf1N1B4_2553 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-), at 2,896,170 to 2,896,817 _2553 Pf1N1B4_2554 - Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) (EC 3.6.1.17), at 2,897,002 to 2,897,340 _2554 Pf1N1B4_2555 - Oxidoreductase, short chain dehydrogenase/reductase family, at 2,897,423 to 2,898,223 _2555 Pf1N1B4_2556 - FIG00954311: hypothetical protein, at 2,898,274 to 2,899,251 _2556 Position (kb) 2897 2898 2899Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2896.851 kb on + strandat 2896.852 kb on - strandat 2896.852 kb on - strandat 2896.955 kb on + strandat 2896.955 kb on + strandat 2897.046 kb on + strand, within Pf1N1B4_2554at 2897.046 kb on + strand, within Pf1N1B4_2554at 2897.046 kb on + strand, within Pf1N1B4_2554at 2897.047 kb on - strand, within Pf1N1B4_2554at 2897.047 kb on - strand, within Pf1N1B4_2554at 2897.047 kb on - strandat 2897.057 kb on + strand, within Pf1N1B4_2554at 2897.058 kb on - strand, within Pf1N1B4_2554at 2897.058 kb on - strandat 2897.058 kb on - strand, within Pf1N1B4_2554at 2897.154 kb on + strand, within Pf1N1B4_2554at 2897.154 kb on + strand, within Pf1N1B4_2554at 2897.155 kb on - strand, within Pf1N1B4_2554at 2897.173 kb on - strand, within Pf1N1B4_2554at 2897.222 kb on - strand, within Pf1N1B4_2554at 2897.234 kb on - strand, within Pf1N1B4_2554at 2897.269 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.286 kb on - strand, within Pf1N1B4_2554at 2897.286 kb on - strand, within Pf1N1B4_2554at 2897.286 kb on - strand, within Pf1N1B4_2554at 2897.286 kb on - strand, within Pf1N1B4_2554at 2897.317 kb on - strandat 2897.317 kb on - strandat 2897.334 kb on + strandat 2897.377 kb on - strandat 2897.387 kb on + strandat 2897.436 kb on + strandat 2897.437 kb on - strandat 2897.437 kb on - strandat 2897.437 kb on - strandat 2897.437 kb on - strandat 2897.533 kb on + strand, within Pf1N1B4_2555at 2897.533 kb on + strandat 2897.534 kb on - strand, within Pf1N1B4_2555at 2897.534 kb on - strand, within Pf1N1B4_2555at 2897.568 kb on + strand, within Pf1N1B4_2555at 2897.568 kb on + strand, within Pf1N1B4_2555at 2897.568 kb on + strand, within Pf1N1B4_2555at 2897.568 kb on + strand, within Pf1N1B4_2555at 2897.568 kb on + strand, within Pf1N1B4_2555at 2897.569 kb on - strand, within Pf1N1B4_2555at 2897.569 kb on - strand, within Pf1N1B4_2555at 2897.569 kb on - strand, within Pf1N1B4_2555at 2897.569 kb on - strand, within Pf1N1B4_2555at 2897.569 kb on - strand, within Pf1N1B4_2555at 2897.739 kb on + strand, within Pf1N1B4_2555at 2897.739 kb on + strand, within Pf1N1B4_2555at 2897.739 kb on + strand, within Pf1N1B4_2555at 2897.739 kb on + strand, within Pf1N1B4_2555at 2897.740 kb on - strand, within Pf1N1B4_2555at 2897.740 kb on - strand, within Pf1N1B4_2555at 2897.755 kb on - strandat 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2898.292 kb on + strandat 2898.292 kb on + strandat 2898.293 kb on - strandat 2898.293 kb on - strandat 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.402 kb on - strand, within Pf1N1B4_2556at 2898.402 kb on - strand, within Pf1N1B4_2556at 2898.402 kb on - strand, within Pf1N1B4_2556at 2898.402 kb on - strand, within Pf1N1B4_2556at 2898.525 kb on + strand, within Pf1N1B4_2556at 2898.583 kb on + strand, within Pf1N1B4_2556at 2898.584 kb on - strand, within Pf1N1B4_2556at 2898.586 kb on + strand, within Pf1N1B4_2556at 2898.586 kb on + strand, within Pf1N1B4_2556at 2898.586 kb on + strandat 2898.586 kb on + strand, within Pf1N1B4_2556at 2898.586 kb on + strand, within Pf1N1B4_2556at 2898.586 kb on + strand, within Pf1N1B4_2556at 2898.587 kb on - strand, within Pf1N1B4_2556at 2898.587 kb on - strand, within Pf1N1B4_2556at 2898.762 kb on + strand, within Pf1N1B4_2556at 2898.935 kb on + strand, within Pf1N1B4_2556at 2898.935 kb on + strand, within Pf1N1B4_2556at 2898.935 kb on + strand, within Pf1N1B4_2556at 2898.936 kb on - strand, within Pf1N1B4_2556at 2898.936 kb on - strand, within Pf1N1B4_2556at 2899.030 kb on + strand, within Pf1N1B4_2556at 2899.030 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.071 kb on - strandat 2899.071 kb on - strand, within Pf1N1B4_2556at 2899.071 kb on - strand, within Pf1N1B4_2556at 2899.071 kb on - strand, within Pf1N1B4_2556

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3
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2,896,851 + -3.5
2,896,852 - -2.5
2,896,852 - -0.0
2,896,955 + -0.0
2,896,955 + -0.0
2,897,046 + Pf1N1B4_2554 0.13 -3.0
2,897,046 + Pf1N1B4_2554 0.13 -0.0
2,897,046 + Pf1N1B4_2554 0.13 -3.1
2,897,047 - Pf1N1B4_2554 0.13 -0.0
2,897,047 - Pf1N1B4_2554 0.13 -0.0
2,897,047 - -0.0
2,897,057 + Pf1N1B4_2554 0.16 -3.4
2,897,058 - Pf1N1B4_2554 0.17 -0.0
2,897,058 - -3.3
2,897,058 - Pf1N1B4_2554 0.17 -0.0
2,897,154 + Pf1N1B4_2554 0.45 -2.3
2,897,154 + Pf1N1B4_2554 0.45 -2.9
2,897,155 - Pf1N1B4_2554 0.45 -0.0
2,897,173 - Pf1N1B4_2554 0.50 -0.0
2,897,222 - Pf1N1B4_2554 0.65 -0.0
2,897,234 - Pf1N1B4_2554 0.68 -3.7
2,897,269 + Pf1N1B4_2554 0.79 -2.8
2,897,285 + Pf1N1B4_2554 0.83 -1.3
2,897,285 + Pf1N1B4_2554 0.83 -2.7
2,897,285 + Pf1N1B4_2554 0.83 -0.0
2,897,285 + Pf1N1B4_2554 0.83 -2.8
2,897,285 + Pf1N1B4_2554 0.83 -2.5
2,897,285 + Pf1N1B4_2554 0.83 -0.0
2,897,285 + Pf1N1B4_2554 0.83 -0.0
2,897,286 - Pf1N1B4_2554 0.84 -0.0
2,897,286 - Pf1N1B4_2554 0.84 -0.0
2,897,286 - Pf1N1B4_2554 0.84 -0.0
2,897,286 - Pf1N1B4_2554 0.84 -0.0
2,897,317 - -0.0
2,897,317 - -0.0
2,897,334 + -0.0
2,897,377 - -2.6
2,897,387 + -1.8
2,897,436 + -0.0
2,897,437 - -0.0
2,897,437 - -0.0
2,897,437 - -0.0
2,897,437 - -3.8
2,897,533 + Pf1N1B4_2555 0.14 -3.0
2,897,533 + -0.0
2,897,534 - Pf1N1B4_2555 0.14 -0.0
2,897,534 - Pf1N1B4_2555 0.14 -2.4
2,897,568 + Pf1N1B4_2555 0.18 -0.0
2,897,568 + Pf1N1B4_2555 0.18 -0.0
2,897,568 + Pf1N1B4_2555 0.18 -2.9
2,897,568 + Pf1N1B4_2555 0.18 -0.0
2,897,568 + Pf1N1B4_2555 0.18 -2.4
2,897,569 - Pf1N1B4_2555 0.18 -0.0
2,897,569 - Pf1N1B4_2555 0.18 -0.0
2,897,569 - Pf1N1B4_2555 0.18 -0.0
2,897,569 - Pf1N1B4_2555 0.18 -2.1
2,897,569 - Pf1N1B4_2555 0.18 -2.2
2,897,739 + Pf1N1B4_2555 0.39 -0.0
2,897,739 + Pf1N1B4_2555 0.39 -2.2
2,897,739 + Pf1N1B4_2555 0.39 -0.0
2,897,739 + Pf1N1B4_2555 0.39 -0.0
2,897,740 - Pf1N1B4_2555 0.40 -0.0
2,897,740 - Pf1N1B4_2555 0.40 -0.0
2,897,755 - -1.7
2,897,757 + Pf1N1B4_2555 0.42 -0.0
2,897,757 + Pf1N1B4_2555 0.42 -2.2
2,897,757 + Pf1N1B4_2555 0.42 -1.8
2,897,757 + Pf1N1B4_2555 0.42 -2.2
2,897,757 + Pf1N1B4_2555 0.42 -0.0
2,897,757 + Pf1N1B4_2555 0.42 -0.0
2,897,758 - Pf1N1B4_2555 0.42 -0.0
2,897,758 - Pf1N1B4_2555 0.42 -0.0
2,897,758 - Pf1N1B4_2555 0.42 -0.0
2,897,758 - Pf1N1B4_2555 0.42 -0.0
2,897,758 - Pf1N1B4_2555 0.42 -0.0
2,897,758 - Pf1N1B4_2555 0.42 -0.0
2,897,758 - Pf1N1B4_2555 0.42 -3.3
2,897,758 - Pf1N1B4_2555 0.42 -0.0
2,897,758 - Pf1N1B4_2555 0.42 -3.6
2,897,758 - Pf1N1B4_2555 0.42 -3.1
2,897,758 - Pf1N1B4_2555 0.42 -3.6
2,898,292 + -0.0
2,898,292 + -2.6
2,898,293 - -2.6
2,898,293 - -0.0
2,898,401 + Pf1N1B4_2556 0.13 -0.0
2,898,401 + Pf1N1B4_2556 0.13 -0.0
2,898,401 + Pf1N1B4_2556 0.13 -0.0
2,898,401 + Pf1N1B4_2556 0.13 -0.0
2,898,401 + Pf1N1B4_2556 0.13 -0.0
2,898,401 + Pf1N1B4_2556 0.13 -0.4
2,898,401 + Pf1N1B4_2556 0.13 -0.0
2,898,402 - Pf1N1B4_2556 0.13 -3.5
2,898,402 - Pf1N1B4_2556 0.13 -0.0
2,898,402 - Pf1N1B4_2556 0.13 -0.0
2,898,402 - Pf1N1B4_2556 0.13 -2.3
2,898,525 + Pf1N1B4_2556 0.26 -1.0
2,898,583 + Pf1N1B4_2556 0.32 -0.0
2,898,584 - Pf1N1B4_2556 0.32 -0.0
2,898,586 + Pf1N1B4_2556 0.32 -0.0
2,898,586 + Pf1N1B4_2556 0.32 -2.5
2,898,586 + -4.8
2,898,586 + Pf1N1B4_2556 0.32 -0.0
2,898,586 + Pf1N1B4_2556 0.32 -0.0
2,898,586 + Pf1N1B4_2556 0.32 -0.0
2,898,587 - Pf1N1B4_2556 0.32 -2.3
2,898,587 - Pf1N1B4_2556 0.32 -2.4
2,898,762 + Pf1N1B4_2556 0.50 -0.0
2,898,935 + Pf1N1B4_2556 0.68 -0.0
2,898,935 + Pf1N1B4_2556 0.68 -0.0
2,898,935 + Pf1N1B4_2556 0.68 -1.0
2,898,936 - Pf1N1B4_2556 0.68 -2.6
2,898,936 - Pf1N1B4_2556 0.68 -0.0
2,899,030 + Pf1N1B4_2556 0.77 -3.2
2,899,030 + Pf1N1B4_2556 0.77 -3.0
2,899,070 + Pf1N1B4_2556 0.81 -3.8
2,899,070 + Pf1N1B4_2556 0.81 -0.0
2,899,070 + Pf1N1B4_2556 0.81 -0.0
2,899,070 + Pf1N1B4_2556 0.81 -3.1
2,899,070 + Pf1N1B4_2556 0.81 -0.0
2,899,070 + Pf1N1B4_2556 0.81 -0.0
2,899,071 - -2.7
2,899,071 - Pf1N1B4_2556 0.81 -0.7
2,899,071 - Pf1N1B4_2556 0.81 -0.0
2,899,071 - Pf1N1B4_2556 0.81 -0.0

Or see this region's nucleotide sequence