Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2354 and Pf1N1B4_2355 are separated by 10 nucleotides Pf1N1B4_2355 and Pf1N1B4_2356 overlap by 20 nucleotides Pf1N1B4_2356 and Pf1N1B4_2357 are separated by 27 nucleotides Pf1N1B4_2357 and Pf1N1B4_2358 are separated by 36 nucleotides
Pf1N1B4_2354 - twitching motility protein PilH, at 2,667,917 to 2,668,282
_2354
Pf1N1B4_2355 - type IV pili signal transduction protein PilI, at 2,668,293 to 2,668,826
_2355
Pf1N1B4_2356 - hypothetical protein, at 2,668,807 to 2,668,929
_2356
Pf1N1B4_2357 - type IV pilus biogenesis protein PilJ, at 2,668,957 to 2,671,014
_2357
Pf1N1B4_2358 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 2,671,051 to 2,676,954
_2358
Position (kb)
2668
2669
2670
2671
2672 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2668.160 kb on - strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.702 kb on + strand, within Pf1N1B4_2355 at 2668.702 kb on + strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.706 kb on + strand, within Pf1N1B4_2355 at 2668.706 kb on + strand, within Pf1N1B4_2355 at 2668.707 kb on - strand, within Pf1N1B4_2355 at 2668.771 kb on + strand, within Pf1N1B4_2355 at 2668.772 kb on - strand, within Pf1N1B4_2355 at 2668.823 kb on - strand at 2668.823 kb on - strand at 2668.823 kb on - strand at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.961 kb on + strand at 2668.961 kb on + strand at 2668.962 kb on - strand at 2668.968 kb on + strand at 2669.068 kb on + strand at 2669.069 kb on - strand at 2669.101 kb on + strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.237 kb on - strand, within Pf1N1B4_2357 at 2669.435 kb on - strand, within Pf1N1B4_2357 at 2669.542 kb on - strand, within Pf1N1B4_2357 at 2669.542 kb on - strand, within Pf1N1B4_2357 at 2669.794 kb on - strand, within Pf1N1B4_2357 at 2669.800 kb on - strand, within Pf1N1B4_2357 at 2669.884 kb on - strand, within Pf1N1B4_2357 at 2669.893 kb on + strand, within Pf1N1B4_2357 at 2669.894 kb on - strand, within Pf1N1B4_2357 at 2669.894 kb on - strand, within Pf1N1B4_2357 at 2669.894 kb on - strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2670.115 kb on + strand, within Pf1N1B4_2357 at 2670.115 kb on + strand, within Pf1N1B4_2357 at 2670.115 kb on + strand, within Pf1N1B4_2357 at 2670.115 kb on + strand, within Pf1N1B4_2357 at 2670.115 kb on + strand, within Pf1N1B4_2357 at 2670.116 kb on - strand, within Pf1N1B4_2357 at 2670.116 kb on - strand, within Pf1N1B4_2357 at 2670.116 kb on - strand, within Pf1N1B4_2357 at 2670.116 kb on - strand, within Pf1N1B4_2357 at 2670.116 kb on - strand, within Pf1N1B4_2357 at 2670.593 kb on + strand, within Pf1N1B4_2357 at 2670.593 kb on + strand, within Pf1N1B4_2357 at 2670.594 kb on - strand at 2670.594 kb on - strand, within Pf1N1B4_2357 at 2670.594 kb on - strand, within Pf1N1B4_2357 at 2670.594 kb on - strand, within Pf1N1B4_2357 at 2670.801 kb on + strand, within Pf1N1B4_2357 at 2670.802 kb on - strand, within Pf1N1B4_2357 at 2670.802 kb on - strand, within Pf1N1B4_2357 at 2670.802 kb on - strand, within Pf1N1B4_2357 at 2670.802 kb on - strand, within Pf1N1B4_2357 at 2670.988 kb on - strand at 2671.029 kb on + strand at 2671.029 kb on + strand at 2671.030 kb on - strand at 2671.046 kb on + strand at 2671.046 kb on + strand at 2671.046 kb on + strand at 2671.047 kb on - strand at 2671.047 kb on - strand at 2671.047 kb on - strand at 2671.047 kb on - strand at 2671.047 kb on - strand at 2671.047 kb on - strand at 2671.051 kb on - strand at 2671.069 kb on + strand at 2671.069 kb on + strand at 2671.069 kb on + strand at 2671.069 kb on + strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.281 kb on - strand at 2671.281 kb on - strand at 2671.325 kb on + strand at 2671.354 kb on + strand at 2671.355 kb on - strand at 2671.355 kb on - strand at 2671.355 kb on - strand at 2671.389 kb on - strand at 2671.422 kb on - strand at 2671.422 kb on - strand at 2671.491 kb on - strand at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.863 kb on + strand, within Pf1N1B4_2358 at 2671.863 kb on + strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.916 kb on - strand, within Pf1N1B4_2358
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 2,667,959 + Pf1N1B4_2354 0.11 +0.0 2,667,959 + Pf1N1B4_2354 0.11 +0.0 2,667,959 + Pf1N1B4_2354 0.11 +0.0 2,667,959 + Pf1N1B4_2354 0.11 -4.1 2,667,959 + Pf1N1B4_2354 0.11 +0.0 2,667,960 - Pf1N1B4_2354 0.12 +0.0 2,667,960 - Pf1N1B4_2354 0.12 +0.0 2,667,960 - Pf1N1B4_2354 0.12 -3.7 2,668,160 - Pf1N1B4_2354 0.66 +0.0 2,668,217 + Pf1N1B4_2354 0.82 -2.0 2,668,217 + Pf1N1B4_2354 0.82 +0.0 2,668,217 + Pf1N1B4_2354 0.82 +0.0 2,668,217 + Pf1N1B4_2354 0.82 +0.0 2,668,218 - Pf1N1B4_2354 0.82 +0.0 2,668,218 - Pf1N1B4_2354 0.82 +0.0 2,668,218 - Pf1N1B4_2354 0.82 +0.0 2,668,218 - Pf1N1B4_2354 0.82 +0.0 2,668,218 - Pf1N1B4_2354 0.82 +0.0 2,668,432 - Pf1N1B4_2355 0.26 -2.7 2,668,432 - Pf1N1B4_2355 0.26 +0.0 2,668,432 - Pf1N1B4_2355 0.26 +0.0 2,668,702 + Pf1N1B4_2355 0.77 +0.0 2,668,702 + Pf1N1B4_2355 0.77 +0.0 2,668,703 - Pf1N1B4_2355 0.77 +0.0 2,668,703 - Pf1N1B4_2355 0.77 +0.0 2,668,703 - Pf1N1B4_2355 0.77 +0.0 2,668,706 + Pf1N1B4_2355 0.77 +0.0 2,668,706 + Pf1N1B4_2355 0.77 +0.0 2,668,707 - Pf1N1B4_2355 0.78 +0.0 2,668,771 + Pf1N1B4_2355 0.90 -2.6 2,668,772 - Pf1N1B4_2355 0.90 -0.5 2,668,823 - +0.0 2,668,823 - -1.6 2,668,823 - +0.0 2,668,863 + Pf1N1B4_2356 0.46 -1.0 2,668,863 + Pf1N1B4_2356 0.46 +0.0 2,668,863 + Pf1N1B4_2356 0.46 +0.0 2,668,863 + Pf1N1B4_2356 0.46 +0.0 2,668,863 + Pf1N1B4_2356 0.46 -1.9 2,668,863 + Pf1N1B4_2356 0.46 +0.0 2,668,863 + Pf1N1B4_2356 0.46 +0.0 2,668,863 + Pf1N1B4_2356 0.46 +0.0 2,668,863 + Pf1N1B4_2356 0.46 +0.0 2,668,863 + Pf1N1B4_2356 0.46 +0.0 2,668,864 - Pf1N1B4_2356 0.46 +0.0 2,668,864 - Pf1N1B4_2356 0.46 +0.0 2,668,864 - Pf1N1B4_2356 0.46 +0.0 2,668,864 - Pf1N1B4_2356 0.46 +0.0 2,668,961 + +0.0 2,668,961 + +0.0 2,668,962 - +0.0 2,668,968 + +0.0 2,669,068 + -0.8 2,669,069 - -1.2 2,669,101 + -1.4 2,669,102 - +0.0 2,669,102 - +0.0 2,669,102 - -3.5 2,669,102 - +0.0 2,669,102 - +0.0 2,669,102 - +0.0 2,669,102 - +0.0 2,669,237 - Pf1N1B4_2357 0.14 +0.0 2,669,435 - Pf1N1B4_2357 0.23 +0.0 2,669,542 - Pf1N1B4_2357 0.28 -1.5 2,669,542 - Pf1N1B4_2357 0.28 +0.0 2,669,794 - Pf1N1B4_2357 0.41 +0.0 2,669,800 - Pf1N1B4_2357 0.41 +0.0 2,669,884 - Pf1N1B4_2357 0.45 -1.7 2,669,893 + Pf1N1B4_2357 0.45 +0.0 2,669,894 - Pf1N1B4_2357 0.46 -2.3 2,669,894 - Pf1N1B4_2357 0.46 +0.0 2,669,894 - Pf1N1B4_2357 0.46 +0.0 2,669,897 + Pf1N1B4_2357 0.46 -1.4 2,669,897 + Pf1N1B4_2357 0.46 +0.0 2,669,897 + Pf1N1B4_2357 0.46 +0.0 2,669,897 + Pf1N1B4_2357 0.46 +0.2 2,669,897 + Pf1N1B4_2357 0.46 +0.0 2,669,897 + Pf1N1B4_2357 0.46 +0.1 2,669,898 - Pf1N1B4_2357 0.46 -1.0 2,669,898 - Pf1N1B4_2357 0.46 +0.0 2,669,898 - Pf1N1B4_2357 0.46 +0.0 2,669,898 - Pf1N1B4_2357 0.46 -2.7 2,669,898 - Pf1N1B4_2357 0.46 +0.0 2,669,898 - Pf1N1B4_2357 0.46 +0.0 2,669,898 - Pf1N1B4_2357 0.46 -0.8 2,670,115 + Pf1N1B4_2357 0.56 +0.0 2,670,115 + Pf1N1B4_2357 0.56 +0.3 2,670,115 + Pf1N1B4_2357 0.56 +0.0 2,670,115 + Pf1N1B4_2357 0.56 +0.0 2,670,115 + Pf1N1B4_2357 0.56 +0.0 2,670,116 - Pf1N1B4_2357 0.56 +0.0 2,670,116 - Pf1N1B4_2357 0.56 -2.5 2,670,116 - Pf1N1B4_2357 0.56 +0.0 2,670,116 - Pf1N1B4_2357 0.56 +0.0 2,670,116 - Pf1N1B4_2357 0.56 +0.0 2,670,593 + Pf1N1B4_2357 0.79 +0.0 2,670,593 + Pf1N1B4_2357 0.79 +0.0 2,670,594 - +0.0 2,670,594 - Pf1N1B4_2357 0.80 -1.9 2,670,594 - Pf1N1B4_2357 0.80 +0.0 2,670,594 - Pf1N1B4_2357 0.80 -1.5 2,670,801 + Pf1N1B4_2357 0.90 +0.7 2,670,802 - Pf1N1B4_2357 0.90 -2.5 2,670,802 - Pf1N1B4_2357 0.90 +0.0 2,670,802 - Pf1N1B4_2357 0.90 +0.0 2,670,802 - Pf1N1B4_2357 0.90 +0.0 2,670,988 - +0.0 2,671,029 + +0.0 2,671,029 + +0.0 2,671,030 - -4.8 2,671,046 + -1.4 2,671,046 + -2.0 2,671,046 + +0.0 2,671,047 - +0.0 2,671,047 - -2.3 2,671,047 - -3.6 2,671,047 - +0.0 2,671,047 - +0.0 2,671,047 - -2.4 2,671,051 - -1.6 2,671,069 + -3.7 2,671,069 + +0.0 2,671,069 + -2.4 2,671,069 + -0.4 2,671,070 - +0.0 2,671,070 - +0.0 2,671,070 - -1.0 2,671,070 - +1.0 2,671,070 - +0.0 2,671,070 - +0.0 2,671,070 - -3.0 2,671,070 - +0.0 2,671,070 - +0.0 2,671,070 - -3.6 2,671,070 - +0.0 2,671,070 - -1.8 2,671,281 - -2.0 2,671,281 - +0.0 2,671,325 + -1.5 2,671,354 + +0.0 2,671,355 - +0.0 2,671,355 - -1.8 2,671,355 - +0.0 2,671,389 - +0.0 2,671,422 - +0.0 2,671,422 - -3.4 2,671,491 - +0.0 2,671,861 + Pf1N1B4_2358 0.14 -1.2 2,671,861 + Pf1N1B4_2358 0.14 -2.6 2,671,861 + Pf1N1B4_2358 0.14 +0.0 2,671,861 + Pf1N1B4_2358 0.14 +0.0 2,671,861 + Pf1N1B4_2358 0.14 +0.0 2,671,861 + Pf1N1B4_2358 0.14 -1.0 2,671,861 + Pf1N1B4_2358 0.14 -0.6 2,671,862 - Pf1N1B4_2358 0.14 +0.0 2,671,862 - Pf1N1B4_2358 0.14 -3.2 2,671,862 - Pf1N1B4_2358 0.14 +0.0 2,671,862 - Pf1N1B4_2358 0.14 -2.5 2,671,862 - Pf1N1B4_2358 0.14 -2.3 2,671,862 - Pf1N1B4_2358 0.14 +0.0 2,671,862 - Pf1N1B4_2358 0.14 -1.7 2,671,862 - Pf1N1B4_2358 0.14 +0.0 2,671,862 - Pf1N1B4_2358 0.14 +0.0 2,671,862 - Pf1N1B4_2358 0.14 +0.0 2,671,862 - Pf1N1B4_2358 0.14 +0.0 2,671,862 - Pf1N1B4_2358 0.14 +0.0 2,671,862 - Pf1N1B4_2358 0.14 -2.9 2,671,862 - Pf1N1B4_2358 0.14 +0.0 2,671,862 - Pf1N1B4_2358 0.14 -3.1 2,671,863 + Pf1N1B4_2358 0.14 -1.8 2,671,863 + Pf1N1B4_2358 0.14 -1.8 2,671,864 - Pf1N1B4_2358 0.14 -2.2 2,671,864 - Pf1N1B4_2358 0.14 -2.0 2,671,864 - Pf1N1B4_2358 0.14 +0.0 2,671,864 - Pf1N1B4_2358 0.14 -4.5 2,671,864 - Pf1N1B4_2358 0.14 -3.8 2,671,864 - Pf1N1B4_2358 0.14 +0.0 2,671,916 - Pf1N1B4_2358 0.15 +0.0
Or see this region's nucleotide sequence