Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1556 and Pf1N1B4_1557 are separated by 129 nucleotides Pf1N1B4_1557 and Pf1N1B4_1558 are separated by 59 nucleotides Pf1N1B4_1558 and Pf1N1B4_1559 are separated by 270 nucleotides
Pf1N1B4_1556 - Poly(3-hydroxyalkanoate) depolymerase, at 1,795,068 to 1,795,922
_1556
Pf1N1B4_1557 - Polyhydroxyalkanoic acid synthase, at 1,796,052 to 1,797,734
_1557
Pf1N1B4_1558 - Transcriptional regulator PhaD, at 1,797,794 to 1,798,414
_1558
Pf1N1B4_1559 - Polyhydroxyalkanoate granule-associated protein PhaF, at 1,798,685 to 1,799,473
_1559
Position (kb)
1796
1797
1798 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1795.198 kb on + strand, within Pf1N1B4_1556 at 1795.199 kb on - strand at 1795.199 kb on - strand, within Pf1N1B4_1556 at 1795.335 kb on + strand, within Pf1N1B4_1556 at 1795.335 kb on + strand, within Pf1N1B4_1556 at 1795.515 kb on + strand, within Pf1N1B4_1556 at 1795.515 kb on + strand, within Pf1N1B4_1556 at 1795.516 kb on - strand, within Pf1N1B4_1556 at 1795.516 kb on - strand, within Pf1N1B4_1556 at 1795.516 kb on - strand, within Pf1N1B4_1556 at 1795.557 kb on + strand, within Pf1N1B4_1556 at 1795.558 kb on - strand, within Pf1N1B4_1556 at 1795.638 kb on + strand, within Pf1N1B4_1556 at 1795.638 kb on + strand, within Pf1N1B4_1556 at 1795.639 kb on - strand, within Pf1N1B4_1556 at 1795.639 kb on - strand, within Pf1N1B4_1556 at 1795.639 kb on - strand, within Pf1N1B4_1556 at 1795.888 kb on + strand at 1795.889 kb on - strand at 1795.889 kb on - strand at 1795.942 kb on - strand at 1795.942 kb on - strand at 1795.994 kb on + strand at 1795.995 kb on - strand at 1795.995 kb on - strand at 1796.046 kb on + strand at 1796.046 kb on + strand at 1796.047 kb on - strand at 1796.193 kb on + strand at 1796.193 kb on + strand at 1796.194 kb on - strand at 1796.194 kb on - strand at 1796.194 kb on - strand at 1796.240 kb on - strand, within Pf1N1B4_1557 at 1796.320 kb on - strand, within Pf1N1B4_1557 at 1796.340 kb on + strand, within Pf1N1B4_1557 at 1796.340 kb on + strand, within Pf1N1B4_1557 at 1796.341 kb on - strand, within Pf1N1B4_1557 at 1796.341 kb on - strand, within Pf1N1B4_1557 at 1796.341 kb on - strand, within Pf1N1B4_1557 at 1796.341 kb on - strand, within Pf1N1B4_1557 at 1796.511 kb on - strand, within Pf1N1B4_1557 at 1796.511 kb on - strand, within Pf1N1B4_1557 at 1796.667 kb on + strand, within Pf1N1B4_1557 at 1796.668 kb on - strand, within Pf1N1B4_1557 at 1796.668 kb on - strand, within Pf1N1B4_1557 at 1796.668 kb on - strand, within Pf1N1B4_1557 at 1796.668 kb on - strand, within Pf1N1B4_1557 at 1796.691 kb on + strand, within Pf1N1B4_1557 at 1796.692 kb on - strand, within Pf1N1B4_1557 at 1796.705 kb on - strand, within Pf1N1B4_1557 at 1796.733 kb on + strand, within Pf1N1B4_1557 at 1796.733 kb on + strand, within Pf1N1B4_1557 at 1796.734 kb on - strand, within Pf1N1B4_1557 at 1796.734 kb on - strand, within Pf1N1B4_1557 at 1796.734 kb on - strand, within Pf1N1B4_1557 at 1796.734 kb on - strand, within Pf1N1B4_1557 at 1796.734 kb on - strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.737 kb on - strand, within Pf1N1B4_1557 at 1796.737 kb on - strand, within Pf1N1B4_1557 at 1796.737 kb on - strand, within Pf1N1B4_1557 at 1796.737 kb on - strand, within Pf1N1B4_1557 at 1796.859 kb on + strand, within Pf1N1B4_1557 at 1796.860 kb on - strand at 1796.995 kb on + strand, within Pf1N1B4_1557 at 1796.995 kb on + strand, within Pf1N1B4_1557 at 1796.995 kb on + strand, within Pf1N1B4_1557 at 1796.996 kb on - strand, within Pf1N1B4_1557 at 1796.996 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.046 kb on + strand, within Pf1N1B4_1557 at 1797.047 kb on - strand, within Pf1N1B4_1557 at 1797.047 kb on - strand, within Pf1N1B4_1557 at 1797.186 kb on + strand, within Pf1N1B4_1557 at 1797.186 kb on + strand, within Pf1N1B4_1557 at 1797.186 kb on + strand, within Pf1N1B4_1557 at 1797.187 kb on - strand, within Pf1N1B4_1557 at 1797.187 kb on - strand, within Pf1N1B4_1557 at 1797.197 kb on + strand, within Pf1N1B4_1557 at 1797.198 kb on - strand, within Pf1N1B4_1557 at 1797.201 kb on + strand, within Pf1N1B4_1557 at 1797.201 kb on + strand, within Pf1N1B4_1557 at 1797.201 kb on + strand, within Pf1N1B4_1557 at 1797.202 kb on - strand, within Pf1N1B4_1557 at 1797.202 kb on - strand, within Pf1N1B4_1557 at 1797.202 kb on - strand, within Pf1N1B4_1557 at 1797.241 kb on - strand, within Pf1N1B4_1557 at 1797.432 kb on + strand, within Pf1N1B4_1557 at 1797.445 kb on + strand, within Pf1N1B4_1557 at 1797.446 kb on - strand, within Pf1N1B4_1557 at 1797.446 kb on - strand, within Pf1N1B4_1557 at 1797.493 kb on + strand, within Pf1N1B4_1557 at 1797.493 kb on + strand, within Pf1N1B4_1557 at 1797.493 kb on + strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.576 kb on + strand at 1797.576 kb on + strand at 1797.579 kb on + strand at 1797.580 kb on - strand at 1797.580 kb on - strand at 1797.580 kb on - strand at 1797.580 kb on - strand at 1797.603 kb on - strand at 1797.687 kb on + strand at 1797.688 kb on - strand at 1797.714 kb on - strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.740 kb on - strand at 1797.782 kb on + strand at 1797.782 kb on + strand at 1797.782 kb on + strand at 1797.911 kb on + strand, within Pf1N1B4_1558 at 1797.912 kb on - strand, within Pf1N1B4_1558 at 1797.912 kb on - strand at 1797.915 kb on - strand, within Pf1N1B4_1558 at 1798.026 kb on - strand, within Pf1N1B4_1558 at 1798.275 kb on - strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.445 kb on + strand at 1798.445 kb on + strand at 1798.446 kb on - strand at 1798.526 kb on + strand at 1798.526 kb on + strand at 1798.526 kb on + strand at 1798.526 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 1,795,198 + Pf1N1B4_1556 0.15 -3.2 1,795,199 - -0.0 1,795,199 - Pf1N1B4_1556 0.15 -2.2 1,795,335 + Pf1N1B4_1556 0.31 -0.0 1,795,335 + Pf1N1B4_1556 0.31 -0.0 1,795,515 + Pf1N1B4_1556 0.52 -0.0 1,795,515 + Pf1N1B4_1556 0.52 -0.0 1,795,516 - Pf1N1B4_1556 0.52 -0.0 1,795,516 - Pf1N1B4_1556 0.52 -1.8 1,795,516 - Pf1N1B4_1556 0.52 -0.0 1,795,557 + Pf1N1B4_1556 0.57 -3.1 1,795,558 - Pf1N1B4_1556 0.57 -3.2 1,795,638 + Pf1N1B4_1556 0.67 -0.0 1,795,638 + Pf1N1B4_1556 0.67 -0.0 1,795,639 - Pf1N1B4_1556 0.67 -2.6 1,795,639 - Pf1N1B4_1556 0.67 -0.0 1,795,639 - Pf1N1B4_1556 0.67 -0.0 1,795,888 + -0.0 1,795,889 - -0.0 1,795,889 - -0.0 1,795,942 - -0.0 1,795,942 - -1.0 1,795,994 + -3.4 1,795,995 - -3.1 1,795,995 - -2.6 1,796,046 + -2.5 1,796,046 + -0.0 1,796,047 - -0.0 1,796,193 + -0.0 1,796,193 + -1.2 1,796,194 - -0.0 1,796,194 - -0.0 1,796,194 - -0.0 1,796,240 - Pf1N1B4_1557 0.11 -2.2 1,796,320 - Pf1N1B4_1557 0.16 -2.0 1,796,340 + Pf1N1B4_1557 0.17 -1.8 1,796,340 + Pf1N1B4_1557 0.17 -1.0 1,796,341 - Pf1N1B4_1557 0.17 -0.0 1,796,341 - Pf1N1B4_1557 0.17 -0.0 1,796,341 - Pf1N1B4_1557 0.17 -0.0 1,796,341 - Pf1N1B4_1557 0.17 -0.0 1,796,511 - Pf1N1B4_1557 0.27 -0.0 1,796,511 - Pf1N1B4_1557 0.27 -0.0 1,796,667 + Pf1N1B4_1557 0.37 -0.0 1,796,668 - Pf1N1B4_1557 0.37 -1.2 1,796,668 - Pf1N1B4_1557 0.37 -0.0 1,796,668 - Pf1N1B4_1557 0.37 -0.0 1,796,668 - Pf1N1B4_1557 0.37 -2.2 1,796,691 + Pf1N1B4_1557 0.38 -0.0 1,796,692 - Pf1N1B4_1557 0.38 -0.0 1,796,705 - Pf1N1B4_1557 0.39 -0.0 1,796,733 + Pf1N1B4_1557 0.40 -2.5 1,796,733 + Pf1N1B4_1557 0.40 -0.0 1,796,734 - Pf1N1B4_1557 0.41 -0.0 1,796,734 - Pf1N1B4_1557 0.41 -0.0 1,796,734 - Pf1N1B4_1557 0.41 -0.0 1,796,734 - Pf1N1B4_1557 0.41 -0.0 1,796,734 - Pf1N1B4_1557 0.41 -2.6 1,796,736 + Pf1N1B4_1557 0.41 -0.0 1,796,736 + Pf1N1B4_1557 0.41 -0.0 1,796,736 + Pf1N1B4_1557 0.41 -0.0 1,796,736 + Pf1N1B4_1557 0.41 -0.0 1,796,736 + Pf1N1B4_1557 0.41 -1.7 1,796,736 + Pf1N1B4_1557 0.41 -3.2 1,796,737 - Pf1N1B4_1557 0.41 -0.0 1,796,737 - Pf1N1B4_1557 0.41 -2.1 1,796,737 - Pf1N1B4_1557 0.41 -0.0 1,796,737 - Pf1N1B4_1557 0.41 -0.0 1,796,859 + Pf1N1B4_1557 0.48 -2.4 1,796,860 - -0.6 1,796,995 + Pf1N1B4_1557 0.56 -0.0 1,796,995 + Pf1N1B4_1557 0.56 -0.0 1,796,995 + Pf1N1B4_1557 0.56 -1.1 1,796,996 - Pf1N1B4_1557 0.56 -0.0 1,796,996 - Pf1N1B4_1557 0.56 -1.8 1,797,019 - Pf1N1B4_1557 0.57 -2.8 1,797,019 - Pf1N1B4_1557 0.57 -0.0 1,797,019 - Pf1N1B4_1557 0.57 -0.0 1,797,019 - Pf1N1B4_1557 0.57 -4.0 1,797,019 - Pf1N1B4_1557 0.57 -0.0 1,797,019 - Pf1N1B4_1557 0.57 -0.0 1,797,046 + Pf1N1B4_1557 0.59 -0.0 1,797,047 - Pf1N1B4_1557 0.59 -2.3 1,797,047 - Pf1N1B4_1557 0.59 -0.0 1,797,186 + Pf1N1B4_1557 0.67 -2.2 1,797,186 + Pf1N1B4_1557 0.67 -0.0 1,797,186 + Pf1N1B4_1557 0.67 -0.7 1,797,187 - Pf1N1B4_1557 0.67 -1.5 1,797,187 - Pf1N1B4_1557 0.67 -0.0 1,797,197 + Pf1N1B4_1557 0.68 -2.4 1,797,198 - Pf1N1B4_1557 0.68 -0.0 1,797,201 + Pf1N1B4_1557 0.68 -1.7 1,797,201 + Pf1N1B4_1557 0.68 -0.0 1,797,201 + Pf1N1B4_1557 0.68 -1.8 1,797,202 - Pf1N1B4_1557 0.68 -0.0 1,797,202 - Pf1N1B4_1557 0.68 -0.0 1,797,202 - Pf1N1B4_1557 0.68 -2.1 1,797,241 - Pf1N1B4_1557 0.71 -2.0 1,797,432 + Pf1N1B4_1557 0.82 -0.0 1,797,445 + Pf1N1B4_1557 0.83 -2.2 1,797,446 - Pf1N1B4_1557 0.83 -3.0 1,797,446 - Pf1N1B4_1557 0.83 -0.0 1,797,493 + Pf1N1B4_1557 0.86 -0.0 1,797,493 + Pf1N1B4_1557 0.86 -2.5 1,797,493 + Pf1N1B4_1557 0.86 -0.0 1,797,494 - Pf1N1B4_1557 0.86 -0.0 1,797,494 - Pf1N1B4_1557 0.86 -0.0 1,797,494 - Pf1N1B4_1557 0.86 -0.0 1,797,494 - Pf1N1B4_1557 0.86 -1.6 1,797,494 - Pf1N1B4_1557 0.86 -2.8 1,797,494 - Pf1N1B4_1557 0.86 -1.4 1,797,494 - Pf1N1B4_1557 0.86 -0.0 1,797,535 - Pf1N1B4_1557 0.88 -0.0 1,797,535 - Pf1N1B4_1557 0.88 -0.0 1,797,535 - Pf1N1B4_1557 0.88 -2.7 1,797,535 - Pf1N1B4_1557 0.88 -0.0 1,797,535 - Pf1N1B4_1557 0.88 -0.0 1,797,535 - Pf1N1B4_1557 0.88 -1.6 1,797,535 - Pf1N1B4_1557 0.88 -3.0 1,797,535 - Pf1N1B4_1557 0.88 -0.0 1,797,576 + -0.9 1,797,576 + -0.0 1,797,579 + -1.6 1,797,580 - -0.0 1,797,580 - -2.9 1,797,580 - -0.0 1,797,580 - -0.0 1,797,603 - -0.0 1,797,687 + -0.0 1,797,688 - -0.0 1,797,714 - -1.7 1,797,717 + -0.0 1,797,717 + -2.3 1,797,717 + -0.0 1,797,717 + -0.0 1,797,718 - -1.7 1,797,718 - -0.0 1,797,718 - -0.0 1,797,718 - -0.0 1,797,718 - -1.4 1,797,718 - -0.0 1,797,718 - -3.2 1,797,718 - -1.8 1,797,740 - -0.0 1,797,782 + -0.0 1,797,782 + -2.5 1,797,782 + -4.0 1,797,911 + Pf1N1B4_1558 0.19 -2.7 1,797,912 - Pf1N1B4_1558 0.19 -0.0 1,797,912 - -0.0 1,797,915 - Pf1N1B4_1558 0.19 -0.0 1,798,026 - Pf1N1B4_1558 0.37 -0.0 1,798,275 - Pf1N1B4_1558 0.77 +1.4 1,798,319 + Pf1N1B4_1558 0.85 -5.4 1,798,319 + Pf1N1B4_1558 0.85 -0.0 1,798,319 + Pf1N1B4_1558 0.85 -0.0 1,798,319 + Pf1N1B4_1558 0.85 -5.0 1,798,445 + -2.1 1,798,445 + -0.0 1,798,446 - -0.0 1,798,526 + -0.0 1,798,526 + -0.0 1,798,526 + -0.0 1,798,526 + -0.0
Or see this region's nucleotide sequence