Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1496 and Pf1N1B4_1497 are separated by 34 nucleotides Pf1N1B4_1497 and Pf1N1B4_1498 are separated by 48 nucleotides
Pf1N1B4_1496 - Na(+)/H(+) antiporter, at 1,726,521 to 1,728,263
_1496
Pf1N1B4_1497 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 1,728,298 to 1,731,648
_1497
Pf1N1B4_1498 - Selenoprotein O and cysteine-containing homologs, at 1,731,697 to 1,733,160
_1498
Position (kb)
1728
1729
1730
1731
1732 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1727.607 kb on + strand, within Pf1N1B4_1496 at 1727.607 kb on + strand, within Pf1N1B4_1496 at 1727.608 kb on - strand, within Pf1N1B4_1496 at 1727.608 kb on - strand, within Pf1N1B4_1496 at 1727.608 kb on - strand, within Pf1N1B4_1496 at 1727.924 kb on - strand, within Pf1N1B4_1496 at 1728.161 kb on + strand at 1728.161 kb on + strand at 1728.211 kb on + strand at 1728.211 kb on + strand at 1728.211 kb on + strand at 1728.212 kb on - strand at 1728.261 kb on + strand at 1728.297 kb on + strand at 1728.298 kb on - strand at 1728.298 kb on - strand at 1728.298 kb on - strand at 1728.506 kb on - strand at 1728.625 kb on + strand at 1728.767 kb on + strand, within Pf1N1B4_1497 at 1729.145 kb on - strand, within Pf1N1B4_1497 at 1729.294 kb on + strand, within Pf1N1B4_1497 at 1729.370 kb on - strand, within Pf1N1B4_1497 at 1729.420 kb on + strand, within Pf1N1B4_1497 at 1729.420 kb on + strand, within Pf1N1B4_1497 at 1729.420 kb on + strand, within Pf1N1B4_1497 at 1729.420 kb on + strand, within Pf1N1B4_1497 at 1729.420 kb on + strand, within Pf1N1B4_1497 at 1729.420 kb on + strand, within Pf1N1B4_1497 at 1729.420 kb on + strand, within Pf1N1B4_1497 at 1729.421 kb on - strand, within Pf1N1B4_1497 at 1729.421 kb on - strand, within Pf1N1B4_1497 at 1729.421 kb on - strand, within Pf1N1B4_1497 at 1729.421 kb on - strand, within Pf1N1B4_1497 at 1729.421 kb on - strand, within Pf1N1B4_1497 at 1729.421 kb on - strand, within Pf1N1B4_1497 at 1729.421 kb on - strand, within Pf1N1B4_1497 at 1729.421 kb on - strand, within Pf1N1B4_1497 at 1729.421 kb on - strand, within Pf1N1B4_1497 at 1729.421 kb on - strand, within Pf1N1B4_1497 at 1729.421 kb on - strand, within Pf1N1B4_1497 at 1729.547 kb on + strand, within Pf1N1B4_1497 at 1729.678 kb on + strand, within Pf1N1B4_1497 at 1729.678 kb on + strand, within Pf1N1B4_1497 at 1729.678 kb on + strand, within Pf1N1B4_1497 at 1729.678 kb on + strand, within Pf1N1B4_1497 at 1729.678 kb on + strand, within Pf1N1B4_1497 at 1729.678 kb on + strand, within Pf1N1B4_1497 at 1729.678 kb on + strand, within Pf1N1B4_1497 at 1729.678 kb on + strand, within Pf1N1B4_1497 at 1729.678 kb on + strand, within Pf1N1B4_1497 at 1729.679 kb on - strand, within Pf1N1B4_1497 at 1729.679 kb on - strand, within Pf1N1B4_1497 at 1729.679 kb on - strand, within Pf1N1B4_1497 at 1729.679 kb on - strand, within Pf1N1B4_1497 at 1729.679 kb on - strand, within Pf1N1B4_1497 at 1729.831 kb on + strand, within Pf1N1B4_1497 at 1729.831 kb on + strand, within Pf1N1B4_1497 at 1730.047 kb on + strand, within Pf1N1B4_1497 at 1730.048 kb on - strand, within Pf1N1B4_1497 at 1730.251 kb on + strand, within Pf1N1B4_1497 at 1730.389 kb on + strand, within Pf1N1B4_1497 at 1730.392 kb on + strand, within Pf1N1B4_1497 at 1730.392 kb on + strand, within Pf1N1B4_1497 at 1730.393 kb on - strand, within Pf1N1B4_1497 at 1730.393 kb on - strand, within Pf1N1B4_1497 at 1730.393 kb on - strand, within Pf1N1B4_1497 at 1730.393 kb on - strand, within Pf1N1B4_1497 at 1730.393 kb on - strand, within Pf1N1B4_1497 at 1730.395 kb on + strand, within Pf1N1B4_1497 at 1730.395 kb on + strand, within Pf1N1B4_1497 at 1730.396 kb on - strand, within Pf1N1B4_1497 at 1730.437 kb on + strand, within Pf1N1B4_1497 at 1730.437 kb on + strand, within Pf1N1B4_1497 at 1730.438 kb on - strand, within Pf1N1B4_1497 at 1730.513 kb on - strand, within Pf1N1B4_1497 at 1730.665 kb on + strand, within Pf1N1B4_1497 at 1730.666 kb on - strand, within Pf1N1B4_1497 at 1730.702 kb on - strand, within Pf1N1B4_1497 at 1730.728 kb on + strand, within Pf1N1B4_1497 at 1730.728 kb on + strand, within Pf1N1B4_1497 at 1730.729 kb on - strand, within Pf1N1B4_1497 at 1730.917 kb on + strand, within Pf1N1B4_1497 at 1730.917 kb on + strand, within Pf1N1B4_1497 at 1730.917 kb on + strand, within Pf1N1B4_1497 at 1730.918 kb on - strand, within Pf1N1B4_1497 at 1730.918 kb on - strand, within Pf1N1B4_1497 at 1730.918 kb on - strand, within Pf1N1B4_1497 at 1731.367 kb on + strand at 1731.367 kb on + strand at 1731.367 kb on + strand at 1731.368 kb on - strand at 1731.368 kb on - strand at 1731.368 kb on - strand at 1731.409 kb on + strand at 1731.411 kb on + strand at 1731.412 kb on - strand at 1731.412 kb on - strand at 1731.412 kb on - strand at 1731.553 kb on + strand at 1731.553 kb on + strand at 1731.554 kb on - strand at 1731.554 kb on - strand at 1731.554 kb on - strand at 1731.554 kb on - strand at 1731.926 kb on - strand, within Pf1N1B4_1498 at 1731.949 kb on + strand, within Pf1N1B4_1498 at 1731.949 kb on + strand, within Pf1N1B4_1498 at 1731.949 kb on + strand, within Pf1N1B4_1498 at 1731.949 kb on + strand, within Pf1N1B4_1498 at 1731.950 kb on - strand, within Pf1N1B4_1498 at 1731.950 kb on - strand, within Pf1N1B4_1498 at 1731.950 kb on - strand, within Pf1N1B4_1498 at 1731.950 kb on - strand, within Pf1N1B4_1498 at 1731.950 kb on - strand, within Pf1N1B4_1498 at 1731.997 kb on + strand, within Pf1N1B4_1498 at 1731.997 kb on + strand, within Pf1N1B4_1498 at 1731.997 kb on + strand, within Pf1N1B4_1498 at 1731.998 kb on - strand, within Pf1N1B4_1498 at 1731.998 kb on - strand, within Pf1N1B4_1498 at 1731.998 kb on - strand, within Pf1N1B4_1498 at 1731.998 kb on - strand, within Pf1N1B4_1498 at 1731.998 kb on - strand, within Pf1N1B4_1498 at 1731.998 kb on - strand, within Pf1N1B4_1498 at 1731.999 kb on + strand, within Pf1N1B4_1498 at 1731.999 kb on + strand, within Pf1N1B4_1498 at 1731.999 kb on + strand, within Pf1N1B4_1498 at 1732.000 kb on - strand, within Pf1N1B4_1498 at 1732.000 kb on - strand, within Pf1N1B4_1498 at 1732.055 kb on - strand, within Pf1N1B4_1498 at 1732.267 kb on + strand, within Pf1N1B4_1498 at 1732.267 kb on + strand, within Pf1N1B4_1498 at 1732.267 kb on + strand, within Pf1N1B4_1498 at 1732.268 kb on - strand, within Pf1N1B4_1498 at 1732.268 kb on - strand, within Pf1N1B4_1498 at 1732.268 kb on - strand, within Pf1N1B4_1498 at 1732.270 kb on + strand, within Pf1N1B4_1498 at 1732.357 kb on + strand, within Pf1N1B4_1498 at 1732.358 kb on - strand, within Pf1N1B4_1498 at 1732.358 kb on - strand, within Pf1N1B4_1498 at 1732.358 kb on - strand, within Pf1N1B4_1498 at 1732.421 kb on - strand, within Pf1N1B4_1498 at 1732.555 kb on + strand, within Pf1N1B4_1498
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 1,727,607 + Pf1N1B4_1496 0.62 -2.9 1,727,607 + Pf1N1B4_1496 0.62 -0.0 1,727,608 - Pf1N1B4_1496 0.62 -0.0 1,727,608 - Pf1N1B4_1496 0.62 -0.0 1,727,608 - Pf1N1B4_1496 0.62 -0.0 1,727,924 - Pf1N1B4_1496 0.80 -1.7 1,728,161 + -0.9 1,728,161 + -0.0 1,728,211 + -0.0 1,728,211 + -3.5 1,728,211 + -0.0 1,728,212 - -0.0 1,728,261 + -1.7 1,728,297 + -2.3 1,728,298 - -2.4 1,728,298 - -0.0 1,728,298 - -3.3 1,728,506 - -0.0 1,728,625 + -0.0 1,728,767 + Pf1N1B4_1497 0.14 -0.0 1,729,145 - Pf1N1B4_1497 0.25 -2.8 1,729,294 + Pf1N1B4_1497 0.30 -2.9 1,729,370 - Pf1N1B4_1497 0.32 -0.0 1,729,420 + Pf1N1B4_1497 0.33 -2.6 1,729,420 + Pf1N1B4_1497 0.33 -1.0 1,729,420 + Pf1N1B4_1497 0.33 -3.3 1,729,420 + Pf1N1B4_1497 0.33 -0.0 1,729,420 + Pf1N1B4_1497 0.33 -4.8 1,729,420 + Pf1N1B4_1497 0.33 -0.0 1,729,420 + Pf1N1B4_1497 0.33 -0.0 1,729,421 - Pf1N1B4_1497 0.34 -3.6 1,729,421 - Pf1N1B4_1497 0.34 -0.0 1,729,421 - Pf1N1B4_1497 0.34 -0.0 1,729,421 - Pf1N1B4_1497 0.34 -2.7 1,729,421 - Pf1N1B4_1497 0.34 -0.0 1,729,421 - Pf1N1B4_1497 0.34 -4.4 1,729,421 - Pf1N1B4_1497 0.34 -0.0 1,729,421 - Pf1N1B4_1497 0.34 -3.7 1,729,421 - Pf1N1B4_1497 0.34 -0.0 1,729,421 - Pf1N1B4_1497 0.34 -0.0 1,729,421 - Pf1N1B4_1497 0.34 -0.0 1,729,547 + Pf1N1B4_1497 0.37 -3.2 1,729,678 + Pf1N1B4_1497 0.41 -0.0 1,729,678 + Pf1N1B4_1497 0.41 -0.0 1,729,678 + Pf1N1B4_1497 0.41 -0.0 1,729,678 + Pf1N1B4_1497 0.41 -0.0 1,729,678 + Pf1N1B4_1497 0.41 -0.0 1,729,678 + Pf1N1B4_1497 0.41 -0.0 1,729,678 + Pf1N1B4_1497 0.41 -4.5 1,729,678 + Pf1N1B4_1497 0.41 -0.0 1,729,678 + Pf1N1B4_1497 0.41 -0.0 1,729,679 - Pf1N1B4_1497 0.41 -2.6 1,729,679 - Pf1N1B4_1497 0.41 -0.0 1,729,679 - Pf1N1B4_1497 0.41 -0.0 1,729,679 - Pf1N1B4_1497 0.41 -2.5 1,729,679 - Pf1N1B4_1497 0.41 -2.1 1,729,831 + Pf1N1B4_1497 0.46 -2.6 1,729,831 + Pf1N1B4_1497 0.46 -0.0 1,730,047 + Pf1N1B4_1497 0.52 -0.0 1,730,048 - Pf1N1B4_1497 0.52 -0.0 1,730,251 + Pf1N1B4_1497 0.58 -0.0 1,730,389 + Pf1N1B4_1497 0.62 -0.0 1,730,392 + Pf1N1B4_1497 0.62 -3.1 1,730,392 + Pf1N1B4_1497 0.62 -2.2 1,730,393 - Pf1N1B4_1497 0.63 -0.0 1,730,393 - Pf1N1B4_1497 0.63 -0.0 1,730,393 - Pf1N1B4_1497 0.63 -0.0 1,730,393 - Pf1N1B4_1497 0.63 -3.2 1,730,393 - Pf1N1B4_1497 0.63 -0.0 1,730,395 + Pf1N1B4_1497 0.63 -2.6 1,730,395 + Pf1N1B4_1497 0.63 -2.7 1,730,396 - Pf1N1B4_1497 0.63 -2.5 1,730,437 + Pf1N1B4_1497 0.64 -1.7 1,730,437 + Pf1N1B4_1497 0.64 -0.0 1,730,438 - Pf1N1B4_1497 0.64 -0.0 1,730,513 - Pf1N1B4_1497 0.66 -0.0 1,730,665 + Pf1N1B4_1497 0.71 -0.0 1,730,666 - Pf1N1B4_1497 0.71 -0.0 1,730,702 - Pf1N1B4_1497 0.72 -0.0 1,730,728 + Pf1N1B4_1497 0.73 -0.0 1,730,728 + Pf1N1B4_1497 0.73 -0.0 1,730,729 - Pf1N1B4_1497 0.73 -0.0 1,730,917 + Pf1N1B4_1497 0.78 -2.5 1,730,917 + Pf1N1B4_1497 0.78 -0.0 1,730,917 + Pf1N1B4_1497 0.78 -0.0 1,730,918 - Pf1N1B4_1497 0.78 -3.4 1,730,918 - Pf1N1B4_1497 0.78 -0.0 1,730,918 - Pf1N1B4_1497 0.78 -0.0 1,731,367 + -0.0 1,731,367 + -3.5 1,731,367 + -1.2 1,731,368 - -0.0 1,731,368 - -3.4 1,731,368 - -0.0 1,731,409 + -2.8 1,731,411 + -0.0 1,731,412 - -0.0 1,731,412 - -3.4 1,731,412 - -0.0 1,731,553 + -3.5 1,731,553 + -0.0 1,731,554 - -0.0 1,731,554 - -0.0 1,731,554 - -0.0 1,731,554 - -0.0 1,731,926 - Pf1N1B4_1498 0.16 -0.0 1,731,949 + Pf1N1B4_1498 0.17 -1.6 1,731,949 + Pf1N1B4_1498 0.17 -0.0 1,731,949 + Pf1N1B4_1498 0.17 -0.0 1,731,949 + Pf1N1B4_1498 0.17 -0.0 1,731,950 - Pf1N1B4_1498 0.17 -3.1 1,731,950 - Pf1N1B4_1498 0.17 -1.6 1,731,950 - Pf1N1B4_1498 0.17 -0.0 1,731,950 - Pf1N1B4_1498 0.17 -0.0 1,731,950 - Pf1N1B4_1498 0.17 -0.0 1,731,997 + Pf1N1B4_1498 0.20 -0.0 1,731,997 + Pf1N1B4_1498 0.20 -0.0 1,731,997 + Pf1N1B4_1498 0.20 -5.7 1,731,998 - Pf1N1B4_1498 0.21 -0.0 1,731,998 - Pf1N1B4_1498 0.21 -0.0 1,731,998 - Pf1N1B4_1498 0.21 -0.0 1,731,998 - Pf1N1B4_1498 0.21 -2.8 1,731,998 - Pf1N1B4_1498 0.21 -0.0 1,731,998 - Pf1N1B4_1498 0.21 -2.5 1,731,999 + Pf1N1B4_1498 0.21 -2.5 1,731,999 + Pf1N1B4_1498 0.21 -0.0 1,731,999 + Pf1N1B4_1498 0.21 -0.8 1,732,000 - Pf1N1B4_1498 0.21 -0.0 1,732,000 - Pf1N1B4_1498 0.21 -0.7 1,732,055 - Pf1N1B4_1498 0.24 -3.5 1,732,267 + Pf1N1B4_1498 0.39 -0.0 1,732,267 + Pf1N1B4_1498 0.39 -0.0 1,732,267 + Pf1N1B4_1498 0.39 -0.0 1,732,268 - Pf1N1B4_1498 0.39 -1.6 1,732,268 - Pf1N1B4_1498 0.39 -0.0 1,732,268 - Pf1N1B4_1498 0.39 -1.3 1,732,270 + Pf1N1B4_1498 0.39 -2.7 1,732,357 + Pf1N1B4_1498 0.45 -0.0 1,732,358 - Pf1N1B4_1498 0.45 -0.0 1,732,358 - Pf1N1B4_1498 0.45 -0.0 1,732,358 - Pf1N1B4_1498 0.45 -1.9 1,732,421 - Pf1N1B4_1498 0.49 -1.7 1,732,555 + Pf1N1B4_1498 0.59 -0.0
Or see this region's nucleotide sequence