Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1133

Experiment: Nitrate reduction, replicate C t3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1131 and Pf1N1B4_1132 are separated by 20 nucleotidesPf1N1B4_1132 and Pf1N1B4_1133 are separated by 12 nucleotidesPf1N1B4_1133 and Pf1N1B4_1134 overlap by 4 nucleotides Pf1N1B4_1131 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,269,525 to 1,270,241 _1131 Pf1N1B4_1132 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) / Osmotic adaptation, at 1,270,262 to 1,271,155 _1132 Pf1N1B4_1133 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,271,168 to 1,271,821 _1133 Pf1N1B4_1134 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 1,271,818 to 1,272,975 _1134 Position (kb) 1271 1272Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1270.239 kb on + strandat 1270.239 kb on + strandat 1270.239 kb on + strandat 1270.240 kb on - strandat 1270.240 kb on - strandat 1270.240 kb on - strandat 1270.240 kb on - strandat 1270.278 kb on + strandat 1270.278 kb on + strandat 1270.278 kb on + strandat 1270.279 kb on - strandat 1270.279 kb on - strandat 1270.281 kb on + strandat 1270.281 kb on + strandat 1270.281 kb on + strandat 1270.282 kb on - strandat 1270.282 kb on - strandat 1270.282 kb on - strandat 1270.284 kb on + strandat 1270.285 kb on - strandat 1270.285 kb on - strandat 1270.285 kb on - strandat 1270.398 kb on - strand, within Pf1N1B4_1132at 1270.415 kb on + strand, within Pf1N1B4_1132at 1270.416 kb on - strand, within Pf1N1B4_1132at 1270.523 kb on + strand, within Pf1N1B4_1132at 1270.524 kb on - strand, within Pf1N1B4_1132at 1270.524 kb on - strand, within Pf1N1B4_1132at 1270.524 kb on - strand, within Pf1N1B4_1132at 1270.634 kb on + strand, within Pf1N1B4_1132at 1270.634 kb on + strand, within Pf1N1B4_1132at 1270.635 kb on - strand, within Pf1N1B4_1132at 1270.635 kb on - strand, within Pf1N1B4_1132at 1270.635 kb on - strand, within Pf1N1B4_1132at 1270.635 kb on - strand, within Pf1N1B4_1132at 1270.670 kb on + strand, within Pf1N1B4_1132at 1270.854 kb on - strand, within Pf1N1B4_1132at 1270.854 kb on - strand, within Pf1N1B4_1132at 1270.896 kb on - strandat 1270.896 kb on - strand, within Pf1N1B4_1132at 1270.896 kb on - strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strandat 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.956 kb on - strand, within Pf1N1B4_1132at 1270.956 kb on - strand, within Pf1N1B4_1132at 1270.956 kb on - strand, within Pf1N1B4_1132at 1270.956 kb on - strand, within Pf1N1B4_1132at 1270.967 kb on + strand, within Pf1N1B4_1132at 1270.967 kb on + strand, within Pf1N1B4_1132at 1270.967 kb on + strand, within Pf1N1B4_1132at 1270.998 kb on - strand, within Pf1N1B4_1132at 1271.396 kb on + strand, within Pf1N1B4_1133at 1271.397 kb on - strand, within Pf1N1B4_1133at 1271.483 kb on + strand, within Pf1N1B4_1133at 1271.483 kb on + strandat 1271.483 kb on + strand, within Pf1N1B4_1133at 1271.483 kb on + strand, within Pf1N1B4_1133at 1271.483 kb on + strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.679 kb on + strand, within Pf1N1B4_1133at 1271.679 kb on + strand, within Pf1N1B4_1133at 1271.680 kb on - strand, within Pf1N1B4_1133at 1271.680 kb on - strand, within Pf1N1B4_1133at 1271.680 kb on - strand, within Pf1N1B4_1133at 1272.054 kb on + strandat 1272.054 kb on + strand, within Pf1N1B4_1134at 1272.055 kb on - strand, within Pf1N1B4_1134at 1272.055 kb on - strand, within Pf1N1B4_1134at 1272.064 kb on + strand, within Pf1N1B4_1134at 1272.065 kb on - strand, within Pf1N1B4_1134at 1272.065 kb on - strand, within Pf1N1B4_1134at 1272.211 kb on + strand, within Pf1N1B4_1134at 1272.556 kb on - strand, within Pf1N1B4_1134at 1272.667 kb on + strand, within Pf1N1B4_1134at 1272.706 kb on + strand, within Pf1N1B4_1134at 1272.754 kb on + strand, within Pf1N1B4_1134at 1272.755 kb on - strand, within Pf1N1B4_1134at 1272.755 kb on - strand, within Pf1N1B4_1134at 1272.784 kb on + strand, within Pf1N1B4_1134at 1272.784 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.801 kb on + strand, within Pf1N1B4_1134at 1272.801 kb on + strand, within Pf1N1B4_1134at 1272.801 kb on + strand, within Pf1N1B4_1134at 1272.801 kb on + strand, within Pf1N1B4_1134at 1272.801 kb on + strand, within Pf1N1B4_1134at 1272.802 kb on - strand, within Pf1N1B4_1134at 1272.820 kb on + strand, within Pf1N1B4_1134at 1272.820 kb on + strand, within Pf1N1B4_1134at 1272.820 kb on + strand, within Pf1N1B4_1134at 1272.820 kb on + strand, within Pf1N1B4_1134at 1272.821 kb on - strand, within Pf1N1B4_1134at 1272.821 kb on - strand, within Pf1N1B4_1134

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3
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1,270,239 + -0.0
1,270,239 + -0.0
1,270,239 + -0.0
1,270,240 - -0.0
1,270,240 - -0.0
1,270,240 - -0.0
1,270,240 - -0.0
1,270,278 + -2.4
1,270,278 + -0.0
1,270,278 + -0.0
1,270,279 - -0.0
1,270,279 - -0.0
1,270,281 + -2.9
1,270,281 + -0.0
1,270,281 + -3.4
1,270,282 - -2.2
1,270,282 - -0.0
1,270,282 - -0.0
1,270,284 + -1.7
1,270,285 - -0.0
1,270,285 - -0.0
1,270,285 - -0.0
1,270,398 - Pf1N1B4_1132 0.15 -0.0
1,270,415 + Pf1N1B4_1132 0.17 -0.0
1,270,416 - Pf1N1B4_1132 0.17 -0.0
1,270,523 + Pf1N1B4_1132 0.29 -0.0
1,270,524 - Pf1N1B4_1132 0.29 -0.0
1,270,524 - Pf1N1B4_1132 0.29 -0.0
1,270,524 - Pf1N1B4_1132 0.29 -0.0
1,270,634 + Pf1N1B4_1132 0.42 -0.0
1,270,634 + Pf1N1B4_1132 0.42 -0.0
1,270,635 - Pf1N1B4_1132 0.42 -0.0
1,270,635 - Pf1N1B4_1132 0.42 -0.0
1,270,635 - Pf1N1B4_1132 0.42 -3.1
1,270,635 - Pf1N1B4_1132 0.42 -0.0
1,270,670 + Pf1N1B4_1132 0.46 -3.4
1,270,854 - Pf1N1B4_1132 0.66 -0.0
1,270,854 - Pf1N1B4_1132 0.66 -0.0
1,270,896 - -2.8
1,270,896 - Pf1N1B4_1132 0.71 -0.0
1,270,896 - Pf1N1B4_1132 0.71 -3.2
1,270,955 + Pf1N1B4_1132 0.78 -0.0
1,270,955 + Pf1N1B4_1132 0.78 -0.0
1,270,955 + Pf1N1B4_1132 0.78 -0.0
1,270,955 + Pf1N1B4_1132 0.78 -3.6
1,270,955 + -0.0
1,270,955 + Pf1N1B4_1132 0.78 -0.0
1,270,955 + Pf1N1B4_1132 0.78 -0.0
1,270,955 + Pf1N1B4_1132 0.78 -3.9
1,270,955 + Pf1N1B4_1132 0.78 -0.0
1,270,956 - Pf1N1B4_1132 0.78 -0.0
1,270,956 - Pf1N1B4_1132 0.78 -0.0
1,270,956 - Pf1N1B4_1132 0.78 -0.0
1,270,956 - Pf1N1B4_1132 0.78 -0.0
1,270,967 + Pf1N1B4_1132 0.79 -0.0
1,270,967 + Pf1N1B4_1132 0.79 -0.0
1,270,967 + Pf1N1B4_1132 0.79 -0.0
1,270,998 - Pf1N1B4_1132 0.82 -4.6
1,271,396 + Pf1N1B4_1133 0.35 -1.8
1,271,397 - Pf1N1B4_1133 0.35 -0.0
1,271,483 + Pf1N1B4_1133 0.48 -0.0
1,271,483 + -2.2
1,271,483 + Pf1N1B4_1133 0.48 -0.0
1,271,483 + Pf1N1B4_1133 0.48 -2.5
1,271,483 + Pf1N1B4_1133 0.48 -2.3
1,271,484 - Pf1N1B4_1133 0.48 -4.8
1,271,484 - Pf1N1B4_1133 0.48 -3.0
1,271,484 - Pf1N1B4_1133 0.48 -3.4
1,271,484 - Pf1N1B4_1133 0.48 -3.1
1,271,484 - Pf1N1B4_1133 0.48 -0.0
1,271,484 - Pf1N1B4_1133 0.48 -0.0
1,271,679 + Pf1N1B4_1133 0.78 -0.0
1,271,679 + Pf1N1B4_1133 0.78 -0.0
1,271,680 - Pf1N1B4_1133 0.78 -0.0
1,271,680 - Pf1N1B4_1133 0.78 -2.7
1,271,680 - Pf1N1B4_1133 0.78 -0.0
1,272,054 + -0.0
1,272,054 + Pf1N1B4_1134 0.20 -0.0
1,272,055 - Pf1N1B4_1134 0.20 -0.0
1,272,055 - Pf1N1B4_1134 0.20 -4.1
1,272,064 + Pf1N1B4_1134 0.21 -3.4
1,272,065 - Pf1N1B4_1134 0.21 -0.0
1,272,065 - Pf1N1B4_1134 0.21 -0.0
1,272,211 + Pf1N1B4_1134 0.34 -0.0
1,272,556 - Pf1N1B4_1134 0.64 -3.0
1,272,667 + Pf1N1B4_1134 0.73 -0.0
1,272,706 + Pf1N1B4_1134 0.77 -3.4
1,272,754 + Pf1N1B4_1134 0.81 -2.7
1,272,755 - Pf1N1B4_1134 0.81 -2.6
1,272,755 - Pf1N1B4_1134 0.81 -1.8
1,272,784 + Pf1N1B4_1134 0.83 -0.0
1,272,784 + Pf1N1B4_1134 0.83 -0.0
1,272,797 + Pf1N1B4_1134 0.85 -2.1
1,272,797 + Pf1N1B4_1134 0.85 -0.0
1,272,797 + Pf1N1B4_1134 0.85 -5.0
1,272,797 + Pf1N1B4_1134 0.85 -0.0
1,272,797 + Pf1N1B4_1134 0.85 -0.0
1,272,797 + Pf1N1B4_1134 0.85 -1.0
1,272,797 + Pf1N1B4_1134 0.85 -1.8
1,272,797 + Pf1N1B4_1134 0.85 -0.0
1,272,797 + Pf1N1B4_1134 0.85 -0.0
1,272,797 + Pf1N1B4_1134 0.85 -4.1
1,272,797 + Pf1N1B4_1134 0.85 -2.8
1,272,798 - Pf1N1B4_1134 0.85 -0.0
1,272,798 - Pf1N1B4_1134 0.85 -3.8
1,272,798 - Pf1N1B4_1134 0.85 -0.0
1,272,798 - Pf1N1B4_1134 0.85 -0.0
1,272,798 - Pf1N1B4_1134 0.85 -1.5
1,272,798 - Pf1N1B4_1134 0.85 -0.0
1,272,798 - Pf1N1B4_1134 0.85 -0.0
1,272,801 + Pf1N1B4_1134 0.85 -0.0
1,272,801 + Pf1N1B4_1134 0.85 -0.0
1,272,801 + Pf1N1B4_1134 0.85 -0.0
1,272,801 + Pf1N1B4_1134 0.85 -0.0
1,272,801 + Pf1N1B4_1134 0.85 -0.0
1,272,802 - Pf1N1B4_1134 0.85 -0.0
1,272,820 + Pf1N1B4_1134 0.87 -0.0
1,272,820 + Pf1N1B4_1134 0.87 -0.0
1,272,820 + Pf1N1B4_1134 0.87 -2.6
1,272,820 + Pf1N1B4_1134 0.87 -0.0
1,272,821 - Pf1N1B4_1134 0.87 -0.0
1,272,821 - Pf1N1B4_1134 0.87 -2.4

Or see this region's nucleotide sequence