Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1038 and Pf1N1B4_1039 are separated by 206 nucleotides Pf1N1B4_1039 and Pf1N1B4_1040 are separated by 89 nucleotides Pf1N1B4_1040 and Pf1N1B4_1041 are separated by 22 nucleotides Pf1N1B4_1041 and Pf1N1B4_1042 are separated by 2 nucleotides
Pf1N1B4_1038 - Transcriptional regulator, MerR family, at 1,176,975 to 1,177,934
_1038
Pf1N1B4_1039 - putative membrane protein, at 1,178,141 to 1,178,710
_1039
Pf1N1B4_1040 - FolM Alternative dihydrofolate reductase 1, at 1,178,800 to 1,179,510
_1040
Pf1N1B4_1041 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 1,179,533 to 1,180,108
_1041
Pf1N1B4_1042 - Dihydroneopterin triphosphate epimerase, at 1,180,111 to 1,180,482
_1042
Position (kb)
1178
1179
1180 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1177.818 kb on + strand, within Pf1N1B4_1038 at 1177.942 kb on + strand at 1177.942 kb on + strand at 1177.942 kb on + strand at 1177.942 kb on + strand at 1177.943 kb on - strand at 1177.943 kb on - strand at 1177.952 kb on + strand at 1177.952 kb on + strand at 1177.952 kb on + strand at 1177.953 kb on - strand at 1178.010 kb on + strand at 1178.011 kb on - strand at 1178.105 kb on - strand at 1178.146 kb on + strand at 1178.147 kb on - strand at 1178.272 kb on - strand, within Pf1N1B4_1039 at 1178.308 kb on + strand, within Pf1N1B4_1039 at 1178.309 kb on - strand, within Pf1N1B4_1039 at 1178.309 kb on - strand, within Pf1N1B4_1039 at 1178.532 kb on + strand, within Pf1N1B4_1039 at 1178.533 kb on - strand, within Pf1N1B4_1039 at 1178.612 kb on + strand, within Pf1N1B4_1039 at 1178.612 kb on + strand, within Pf1N1B4_1039 at 1178.613 kb on - strand, within Pf1N1B4_1039 at 1178.613 kb on - strand, within Pf1N1B4_1039 at 1178.613 kb on - strand, within Pf1N1B4_1039 at 1178.613 kb on - strand, within Pf1N1B4_1039 at 1178.613 kb on - strand, within Pf1N1B4_1039 at 1178.718 kb on + strand at 1178.719 kb on - strand at 1178.719 kb on - strand at 1178.742 kb on + strand at 1178.742 kb on + strand at 1178.742 kb on + strand at 1178.742 kb on + strand at 1178.742 kb on + strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.767 kb on - strand at 1178.769 kb on - strand at 1178.769 kb on - strand at 1178.781 kb on - strand at 1178.808 kb on + strand at 1178.809 kb on - strand at 1179.120 kb on + strand, within Pf1N1B4_1040 at 1179.120 kb on + strand, within Pf1N1B4_1040 at 1179.120 kb on + strand, within Pf1N1B4_1040 at 1179.121 kb on - strand, within Pf1N1B4_1040 at 1179.121 kb on - strand, within Pf1N1B4_1040 at 1179.121 kb on - strand at 1179.338 kb on + strand, within Pf1N1B4_1040 at 1179.338 kb on + strand at 1179.339 kb on - strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.442 kb on + strand at 1179.460 kb on + strand at 1179.460 kb on + strand at 1179.460 kb on + strand at 1179.461 kb on - strand at 1179.461 kb on - strand at 1179.461 kb on - strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.541 kb on - strand at 1179.573 kb on - strand at 1179.659 kb on + strand, within Pf1N1B4_1041 at 1179.659 kb on + strand at 1179.659 kb on + strand, within Pf1N1B4_1041 at 1179.660 kb on - strand, within Pf1N1B4_1041 at 1179.660 kb on - strand at 1179.660 kb on - strand, within Pf1N1B4_1041 at 1179.660 kb on - strand, within Pf1N1B4_1041 at 1179.674 kb on + strand, within Pf1N1B4_1041 at 1179.675 kb on - strand, within Pf1N1B4_1041 at 1179.761 kb on + strand, within Pf1N1B4_1041 at 1179.761 kb on + strand, within Pf1N1B4_1041 at 1179.762 kb on - strand, within Pf1N1B4_1041 at 1179.762 kb on - strand, within Pf1N1B4_1041 at 1179.815 kb on + strand, within Pf1N1B4_1041 at 1179.815 kb on + strand, within Pf1N1B4_1041 at 1179.815 kb on + strand at 1179.815 kb on + strand, within Pf1N1B4_1041 at 1179.815 kb on + strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.817 kb on + strand, within Pf1N1B4_1041 at 1179.818 kb on - strand, within Pf1N1B4_1041 at 1179.818 kb on - strand, within Pf1N1B4_1041 at 1179.868 kb on + strand, within Pf1N1B4_1041 at 1179.868 kb on + strand, within Pf1N1B4_1041 at 1179.868 kb on + strand, within Pf1N1B4_1041 at 1179.868 kb on + strand, within Pf1N1B4_1041 at 1179.869 kb on - strand, within Pf1N1B4_1041 at 1179.869 kb on - strand, within Pf1N1B4_1041 at 1179.869 kb on - strand, within Pf1N1B4_1041 at 1179.869 kb on - strand, within Pf1N1B4_1041 at 1180.063 kb on + strand at 1180.064 kb on - strand at 1180.064 kb on - strand at 1180.067 kb on - strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.107 kb on - strand at 1180.107 kb on - strand at 1180.107 kb on - strand at 1180.107 kb on - strand at 1180.107 kb on - strand at 1180.176 kb on - strand, within Pf1N1B4_1042 at 1180.288 kb on + strand, within Pf1N1B4_1042 at 1180.288 kb on + strand, within Pf1N1B4_1042 at 1180.402 kb on + strand, within Pf1N1B4_1042 at 1180.402 kb on + strand, within Pf1N1B4_1042 at 1180.507 kb on + strand at 1180.508 kb on - strand at 1180.508 kb on - strand at 1180.508 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 1,177,818 + Pf1N1B4_1038 0.88 -0.1 1,177,942 + -0.1 1,177,942 + -0.1 1,177,942 + -0.1 1,177,942 + -3.5 1,177,943 - -2.5 1,177,943 - -0.1 1,177,952 + -0.1 1,177,952 + -2.6 1,177,952 + -3.0 1,177,953 - -0.1 1,178,010 + -3.1 1,178,011 - -0.1 1,178,105 - -0.1 1,178,146 + -0.1 1,178,147 - -3.3 1,178,272 - Pf1N1B4_1039 0.23 -0.1 1,178,308 + Pf1N1B4_1039 0.29 -0.1 1,178,309 - Pf1N1B4_1039 0.29 -0.1 1,178,309 - Pf1N1B4_1039 0.29 -0.1 1,178,532 + Pf1N1B4_1039 0.69 -0.1 1,178,533 - Pf1N1B4_1039 0.69 -2.4 1,178,612 + Pf1N1B4_1039 0.83 -0.1 1,178,612 + Pf1N1B4_1039 0.83 -0.1 1,178,613 - Pf1N1B4_1039 0.83 -0.1 1,178,613 - Pf1N1B4_1039 0.83 -2.1 1,178,613 - Pf1N1B4_1039 0.83 -0.1 1,178,613 - Pf1N1B4_1039 0.83 +0.9 1,178,613 - Pf1N1B4_1039 0.83 -3.5 1,178,718 + -2.4 1,178,719 - -0.1 1,178,719 - -0.1 1,178,742 + -0.1 1,178,742 + -0.1 1,178,742 + -2.1 1,178,742 + -0.1 1,178,742 + -0.1 1,178,743 - -0.9 1,178,743 - -0.1 1,178,743 - -1.6 1,178,743 - -2.5 1,178,743 - -0.1 1,178,743 - -0.1 1,178,743 - -3.2 1,178,743 - -2.2 1,178,767 - -0.1 1,178,769 - -0.1 1,178,769 - -0.1 1,178,781 - -0.1 1,178,808 + -0.1 1,178,809 - -0.1 1,179,120 + Pf1N1B4_1040 0.45 -2.1 1,179,120 + Pf1N1B4_1040 0.45 +0.3 1,179,120 + Pf1N1B4_1040 0.45 -0.1 1,179,121 - Pf1N1B4_1040 0.45 -3.8 1,179,121 - Pf1N1B4_1040 0.45 -0.1 1,179,121 - -0.1 1,179,338 + Pf1N1B4_1040 0.76 -0.1 1,179,338 + +3.3 1,179,339 - Pf1N1B4_1040 0.76 -0.1 1,179,427 + Pf1N1B4_1040 0.88 -0.1 1,179,427 + Pf1N1B4_1040 0.88 -0.2 1,179,427 + Pf1N1B4_1040 0.88 -0.1 1,179,427 + Pf1N1B4_1040 0.88 -0.1 1,179,428 - Pf1N1B4_1040 0.88 -0.1 1,179,428 - Pf1N1B4_1040 0.88 -0.1 1,179,428 - Pf1N1B4_1040 0.88 -1.8 1,179,428 - Pf1N1B4_1040 0.88 -0.1 1,179,428 - Pf1N1B4_1040 0.88 -0.1 1,179,442 + -0.1 1,179,460 + +1.2 1,179,460 + -0.1 1,179,460 + -0.1 1,179,461 - -2.6 1,179,461 - -0.1 1,179,461 - -0.1 1,179,508 + -0.1 1,179,508 + -0.1 1,179,508 + -2.8 1,179,508 + -0.1 1,179,508 + +0.1 1,179,541 - -2.5 1,179,573 - -2.2 1,179,659 + Pf1N1B4_1041 0.22 -0.1 1,179,659 + -0.1 1,179,659 + Pf1N1B4_1041 0.22 -0.1 1,179,660 - Pf1N1B4_1041 0.22 -0.1 1,179,660 - -1.1 1,179,660 - Pf1N1B4_1041 0.22 -0.1 1,179,660 - Pf1N1B4_1041 0.22 -2.8 1,179,674 + Pf1N1B4_1041 0.24 -0.1 1,179,675 - Pf1N1B4_1041 0.25 -0.1 1,179,761 + Pf1N1B4_1041 0.40 -0.1 1,179,761 + Pf1N1B4_1041 0.40 -0.1 1,179,762 - Pf1N1B4_1041 0.40 -0.1 1,179,762 - Pf1N1B4_1041 0.40 -2.4 1,179,815 + Pf1N1B4_1041 0.49 -0.1 1,179,815 + Pf1N1B4_1041 0.49 -2.1 1,179,815 + -2.9 1,179,815 + Pf1N1B4_1041 0.49 -0.1 1,179,815 + Pf1N1B4_1041 0.49 -3.0 1,179,816 - Pf1N1B4_1041 0.49 -0.1 1,179,816 - Pf1N1B4_1041 0.49 -0.1 1,179,816 - Pf1N1B4_1041 0.49 -0.1 1,179,816 - Pf1N1B4_1041 0.49 -0.1 1,179,816 - Pf1N1B4_1041 0.49 -0.1 1,179,816 - Pf1N1B4_1041 0.49 -0.1 1,179,816 - Pf1N1B4_1041 0.49 -0.1 1,179,816 - Pf1N1B4_1041 0.49 -3.7 1,179,816 - Pf1N1B4_1041 0.49 -0.1 1,179,816 - Pf1N1B4_1041 0.49 -0.1 1,179,816 - Pf1N1B4_1041 0.49 -2.2 1,179,816 - Pf1N1B4_1041 0.49 -0.1 1,179,816 - Pf1N1B4_1041 0.49 -0.1 1,179,817 + Pf1N1B4_1041 0.49 -0.1 1,179,818 - Pf1N1B4_1041 0.49 -0.1 1,179,818 - Pf1N1B4_1041 0.49 -0.1 1,179,868 + Pf1N1B4_1041 0.58 -1.8 1,179,868 + Pf1N1B4_1041 0.58 -0.1 1,179,868 + Pf1N1B4_1041 0.58 -2.6 1,179,868 + Pf1N1B4_1041 0.58 -2.6 1,179,869 - Pf1N1B4_1041 0.58 -1.4 1,179,869 - Pf1N1B4_1041 0.58 -2.9 1,179,869 - Pf1N1B4_1041 0.58 -0.1 1,179,869 - Pf1N1B4_1041 0.58 -0.1 1,180,063 + -0.1 1,180,064 - -0.1 1,180,064 - -0.1 1,180,067 - -0.1 1,180,106 + -1.7 1,180,106 + -0.1 1,180,106 + -0.1 1,180,106 + -0.1 1,180,106 + -0.1 1,180,106 + -0.1 1,180,106 + -0.1 1,180,107 - -0.1 1,180,107 - -0.1 1,180,107 - -2.4 1,180,107 - -1.6 1,180,107 - -0.1 1,180,176 - Pf1N1B4_1042 0.17 -0.1 1,180,288 + Pf1N1B4_1042 0.48 -2.2 1,180,288 + Pf1N1B4_1042 0.48 -2.2 1,180,402 + Pf1N1B4_1042 0.78 -2.9 1,180,402 + Pf1N1B4_1042 0.78 -1.8 1,180,507 + -3.3 1,180,508 - -2.7 1,180,508 - -2.9 1,180,508 - -0.1
Or see this region's nucleotide sequence