Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_99 and Pf1N1B4_100 are separated by 308 nucleotides Pf1N1B4_100 and Pf1N1B4_101 are separated by 64 nucleotides Pf1N1B4_101 and Pf1N1B4_102 are separated by 374 nucleotides
Pf1N1B4_99 - Purine nucleoside permease, at 104,512 to 105,546
_99
Pf1N1B4_100 - FIG00953437: hypothetical protein, at 105,855 to 106,082
_100
Pf1N1B4_101 - hypothetical protein, at 106,147 to 106,311
_101
Pf1N1B4_102 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 106,686 to 108,446
_102
Position (kb) 105
106
107 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 104.902 kb on + strand, within Pf1N1B4_99 at 104.902 kb on + strand, within Pf1N1B4_99 at 104.903 kb on - strand, within Pf1N1B4_99 at 104.903 kb on - strand at 105.020 kb on - strand, within Pf1N1B4_99 at 105.192 kb on + strand, within Pf1N1B4_99 at 105.192 kb on + strand, within Pf1N1B4_99 at 105.255 kb on + strand, within Pf1N1B4_99 at 105.268 kb on + strand, within Pf1N1B4_99 at 105.268 kb on + strand, within Pf1N1B4_99 at 105.269 kb on - strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.302 kb on - strand at 105.302 kb on - strand, within Pf1N1B4_99 at 105.428 kb on - strand at 105.657 kb on + strand at 105.657 kb on + strand at 105.657 kb on + strand at 105.658 kb on - strand at 105.791 kb on - strand at 105.828 kb on - strand at 105.828 kb on - strand at 105.829 kb on + strand at 105.830 kb on - strand at 105.830 kb on - strand at 105.833 kb on + strand at 105.833 kb on + strand at 105.833 kb on + strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.845 kb on + strand at 105.846 kb on - strand at 105.854 kb on + strand at 105.885 kb on + strand, within Pf1N1B4_100 at 105.885 kb on + strand, within Pf1N1B4_100 at 105.885 kb on + strand, within Pf1N1B4_100 at 105.886 kb on - strand, within Pf1N1B4_100 at 106.226 kb on + strand, within Pf1N1B4_101 at 106.226 kb on + strand, within Pf1N1B4_101 at 106.227 kb on - strand, within Pf1N1B4_101 at 106.293 kb on + strand, within Pf1N1B4_101 at 106.424 kb on + strand at 106.424 kb on + strand at 106.424 kb on + strand at 106.424 kb on + strand at 106.428 kb on - strand at 106.428 kb on - strand at 106.501 kb on + strand at 106.502 kb on - strand at 106.549 kb on + strand at 106.550 kb on - strand at 106.677 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 104,902 + Pf1N1B4_99 0.38 -0.0 104,902 + Pf1N1B4_99 0.38 -2.2 104,903 - Pf1N1B4_99 0.38 -4.2 104,903 - -0.0 105,020 - Pf1N1B4_99 0.49 -0.0 105,192 + Pf1N1B4_99 0.66 -1.0 105,192 + Pf1N1B4_99 0.66 -2.7 105,255 + Pf1N1B4_99 0.72 -0.0 105,268 + Pf1N1B4_99 0.73 -4.0 105,268 + Pf1N1B4_99 0.73 -0.0 105,269 - Pf1N1B4_99 0.73 -1.6 105,301 + Pf1N1B4_99 0.76 -0.0 105,301 + Pf1N1B4_99 0.76 -0.0 105,301 + Pf1N1B4_99 0.76 -0.0 105,301 + Pf1N1B4_99 0.76 -0.0 105,301 + Pf1N1B4_99 0.76 -2.2 105,302 - -0.0 105,302 - Pf1N1B4_99 0.76 -3.0 105,428 - -2.0 105,657 + -2.3 105,657 + -4.4 105,657 + -0.0 105,658 - -1.0 105,791 - -0.0 105,828 - -0.0 105,828 - -1.0 105,829 + -0.0 105,830 - -3.3 105,830 - -3.3 105,833 + -0.0 105,833 + -0.0 105,833 + -0.0 105,834 - -0.0 105,834 - -1.3 105,834 - -0.0 105,834 - -0.0 105,834 - -2.3 105,834 - -2.4 105,834 - -0.0 105,834 - -0.0 105,845 + -0.0 105,846 - -0.0 105,854 + -4.5 105,885 + Pf1N1B4_100 0.13 -3.1 105,885 + Pf1N1B4_100 0.13 -0.0 105,885 + Pf1N1B4_100 0.13 -0.0 105,886 - Pf1N1B4_100 0.14 -0.0 106,226 + Pf1N1B4_101 0.48 -3.2 106,226 + Pf1N1B4_101 0.48 -0.0 106,227 - Pf1N1B4_101 0.48 -4.1 106,293 + Pf1N1B4_101 0.88 -3.0 106,424 + -0.0 106,424 + -0.0 106,424 + -2.8 106,424 + -0.0 106,428 - -2.7 106,428 - -3.0 106,501 + -3.1 106,502 - -0.0 106,549 + -2.1 106,550 - -0.0 106,677 + -3.7
Or see this region's nucleotide sequence