Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05600 and PGA1_c05610 overlap by 1 nucleotides PGA1_c05610 and PGA1_c05620 overlap by 4 nucleotides PGA1_c05620 and PGA1_c05630 are separated by 25 nucleotides PGA1_c05630 and PGA1_c05640 are separated by 37 nucleotides
PGA1_c05600: PGA1_c05600 - queuosine biosynthesis protein QueC, at 574,198 to 574,896
_c05600
PGA1_c05610: PGA1_c05610 - putative queuosine biosynthesis protein QueD, at 574,896 to 575,249
_c05610
PGA1_c05620: PGA1_c05620 - putative 7-cyano-7-deazaguanosine biosynthesis protein QueE, at 575,246 to 576,043
_c05620
PGA1_c05630: PGA1_c05630 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 576,069 to 576,533
_c05630
PGA1_c05640: PGA1_c05640 - hypothetical protein, at 576,571 to 577,242
_c05640
Position (kb) 575
576
577 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 574.272 kb on + strand, within PGA1_c05600 at 574.295 kb on + strand, within PGA1_c05600 at 574.295 kb on + strand, within PGA1_c05600 at 574.295 kb on + strand, within PGA1_c05600 at 574.357 kb on - strand, within PGA1_c05600 at 574.448 kb on + strand, within PGA1_c05600 at 574.451 kb on - strand, within PGA1_c05600 at 574.467 kb on + strand, within PGA1_c05600 at 574.467 kb on + strand, within PGA1_c05600 at 574.467 kb on + strand, within PGA1_c05600 at 574.474 kb on + strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.533 kb on + strand, within PGA1_c05600 at 574.533 kb on + strand, within PGA1_c05600 at 574.566 kb on - strand, within PGA1_c05600 at 574.582 kb on + strand, within PGA1_c05600 at 574.600 kb on + strand, within PGA1_c05600 at 574.604 kb on + strand, within PGA1_c05600 at 574.657 kb on - strand, within PGA1_c05600 at 574.672 kb on + strand, within PGA1_c05600 at 574.693 kb on + strand, within PGA1_c05600 at 574.760 kb on - strand, within PGA1_c05600 at 574.768 kb on + strand, within PGA1_c05600 at 574.803 kb on + strand, within PGA1_c05600 at 574.852 kb on + strand at 574.988 kb on + strand, within PGA1_c05610 at 575.012 kb on + strand, within PGA1_c05610 at 575.017 kb on + strand, within PGA1_c05610 at 575.022 kb on + strand, within PGA1_c05610 at 575.030 kb on - strand, within PGA1_c05610 at 575.030 kb on - strand, within PGA1_c05610 at 575.030 kb on - strand, within PGA1_c05610 at 575.030 kb on - strand, within PGA1_c05610 at 575.035 kb on - strand, within PGA1_c05610 at 575.059 kb on + strand, within PGA1_c05610 at 575.106 kb on + strand, within PGA1_c05610 at 575.124 kb on - strand, within PGA1_c05610 at 575.139 kb on + strand, within PGA1_c05610 at 575.139 kb on + strand, within PGA1_c05610 at 575.139 kb on + strand, within PGA1_c05610 at 575.147 kb on - strand, within PGA1_c05610 at 575.147 kb on - strand, within PGA1_c05610 at 575.187 kb on + strand, within PGA1_c05610 at 575.237 kb on + strand at 575.322 kb on + strand at 575.377 kb on + strand, within PGA1_c05620 at 575.408 kb on + strand, within PGA1_c05620 at 575.432 kb on + strand, within PGA1_c05620 at 575.433 kb on - strand, within PGA1_c05620 at 575.465 kb on - strand, within PGA1_c05620 at 575.465 kb on - strand, within PGA1_c05620 at 575.520 kb on + strand, within PGA1_c05620 at 575.520 kb on + strand, within PGA1_c05620 at 575.525 kb on + strand, within PGA1_c05620 at 575.617 kb on + strand, within PGA1_c05620 at 575.617 kb on + strand, within PGA1_c05620 at 575.617 kb on + strand, within PGA1_c05620 at 575.651 kb on + strand, within PGA1_c05620 at 575.671 kb on + strand, within PGA1_c05620 at 575.684 kb on - strand, within PGA1_c05620 at 575.702 kb on - strand, within PGA1_c05620 at 575.761 kb on + strand, within PGA1_c05620 at 575.761 kb on + strand, within PGA1_c05620 at 575.779 kb on - strand, within PGA1_c05620 at 575.822 kb on + strand, within PGA1_c05620 at 575.884 kb on + strand, within PGA1_c05620 at 575.890 kb on + strand, within PGA1_c05620 at 575.979 kb on + strand at 575.987 kb on - strand at 576.016 kb on + strand at 576.070 kb on + strand at 576.101 kb on + strand at 576.109 kb on + strand at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.122 kb on - strand, within PGA1_c05630 at 576.122 kb on - strand, within PGA1_c05630 at 576.138 kb on - strand, within PGA1_c05630 at 576.148 kb on - strand, within PGA1_c05630 at 576.174 kb on + strand, within PGA1_c05630 at 576.226 kb on - strand, within PGA1_c05630 at 576.226 kb on - strand, within PGA1_c05630 at 576.239 kb on + strand, within PGA1_c05630 at 576.350 kb on + strand, within PGA1_c05630 at 576.466 kb on + strand, within PGA1_c05630 at 576.474 kb on - strand, within PGA1_c05630 at 576.476 kb on + strand, within PGA1_c05630 at 576.484 kb on - strand, within PGA1_c05630 at 576.484 kb on - strand, within PGA1_c05630 at 576.506 kb on - strand at 576.506 kb on - strand at 576.512 kb on + strand at 576.538 kb on + strand at 576.676 kb on + strand, within PGA1_c05640 at 576.714 kb on + strand, within PGA1_c05640 at 576.748 kb on + strand, within PGA1_c05640 at 576.756 kb on - strand, within PGA1_c05640 at 576.756 kb on - strand, within PGA1_c05640 at 576.865 kb on + strand, within PGA1_c05640 at 576.865 kb on + strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 574,272 + PGA1_c05600 0.11 +0.4 574,295 + PGA1_c05600 0.14 -0.7 574,295 + PGA1_c05600 0.14 +0.4 574,295 + PGA1_c05600 0.14 -0.6 574,357 - PGA1_c05600 0.23 +0.9 574,448 + PGA1_c05600 0.36 -0.6 574,451 - PGA1_c05600 0.36 -0.0 574,467 + PGA1_c05600 0.38 +0.7 574,467 + PGA1_c05600 0.38 +0.8 574,467 + PGA1_c05600 0.38 +1.5 574,474 + PGA1_c05600 0.39 +0.4 574,475 - PGA1_c05600 0.40 +1.5 574,475 - PGA1_c05600 0.40 -0.2 574,475 - PGA1_c05600 0.40 +0.4 574,475 - PGA1_c05600 0.40 +0.1 574,533 + PGA1_c05600 0.48 -0.4 574,533 + PGA1_c05600 0.48 +0.7 574,566 - PGA1_c05600 0.53 +1.0 574,582 + PGA1_c05600 0.55 +0.4 574,600 + PGA1_c05600 0.58 -0.2 574,604 + PGA1_c05600 0.58 -0.2 574,657 - PGA1_c05600 0.66 -0.3 574,672 + PGA1_c05600 0.68 +0.7 574,693 + PGA1_c05600 0.71 -0.7 574,760 - PGA1_c05600 0.80 -0.6 574,768 + PGA1_c05600 0.82 +0.4 574,803 + PGA1_c05600 0.87 -0.6 574,852 + +0.1 574,988 + PGA1_c05610 0.26 +0.2 575,012 + PGA1_c05610 0.33 -0.5 575,017 + PGA1_c05610 0.34 -0.1 575,022 + PGA1_c05610 0.36 +1.4 575,030 - PGA1_c05610 0.38 +0.0 575,030 - PGA1_c05610 0.38 +0.4 575,030 - PGA1_c05610 0.38 +0.9 575,030 - PGA1_c05610 0.38 +0.3 575,035 - PGA1_c05610 0.39 -0.2 575,059 + PGA1_c05610 0.46 -0.5 575,106 + PGA1_c05610 0.59 +0.6 575,124 - PGA1_c05610 0.64 +1.5 575,139 + PGA1_c05610 0.69 +0.9 575,139 + PGA1_c05610 0.69 +1.1 575,139 + PGA1_c05610 0.69 -0.3 575,147 - PGA1_c05610 0.71 +1.1 575,147 - PGA1_c05610 0.71 -0.7 575,187 + PGA1_c05610 0.82 +0.5 575,237 + -0.8 575,322 + -0.6 575,377 + PGA1_c05620 0.16 -1.1 575,408 + PGA1_c05620 0.20 -0.3 575,432 + PGA1_c05620 0.23 -1.3 575,433 - PGA1_c05620 0.23 +2.3 575,465 - PGA1_c05620 0.27 +0.4 575,465 - PGA1_c05620 0.27 -1.0 575,520 + PGA1_c05620 0.34 -0.3 575,520 + PGA1_c05620 0.34 +0.8 575,525 + PGA1_c05620 0.35 +0.4 575,617 + PGA1_c05620 0.46 +0.6 575,617 + PGA1_c05620 0.46 +0.6 575,617 + PGA1_c05620 0.46 +0.2 575,651 + PGA1_c05620 0.51 +1.5 575,671 + PGA1_c05620 0.53 -1.5 575,684 - PGA1_c05620 0.55 -1.3 575,702 - PGA1_c05620 0.57 -0.9 575,761 + PGA1_c05620 0.65 +0.5 575,761 + PGA1_c05620 0.65 -0.7 575,779 - PGA1_c05620 0.67 -0.3 575,822 + PGA1_c05620 0.72 +0.7 575,884 + PGA1_c05620 0.80 -0.3 575,890 + PGA1_c05620 0.81 -1.0 575,979 + +1.8 575,987 - -0.9 576,016 + +0.2 576,070 + +0.0 576,101 + +0.7 576,109 + +0.5 576,117 - PGA1_c05630 0.10 +0.3 576,117 - PGA1_c05630 0.10 +0.6 576,117 - PGA1_c05630 0.10 -0.8 576,117 - PGA1_c05630 0.10 -0.1 576,117 - PGA1_c05630 0.10 +0.6 576,117 - PGA1_c05630 0.10 +0.1 576,122 - PGA1_c05630 0.11 -0.6 576,122 - PGA1_c05630 0.11 -0.7 576,138 - PGA1_c05630 0.15 -0.7 576,148 - PGA1_c05630 0.17 +0.0 576,174 + PGA1_c05630 0.23 -0.1 576,226 - PGA1_c05630 0.34 +0.3 576,226 - PGA1_c05630 0.34 +0.4 576,239 + PGA1_c05630 0.37 -0.4 576,350 + PGA1_c05630 0.60 -0.3 576,466 + PGA1_c05630 0.85 +0.7 576,474 - PGA1_c05630 0.87 +0.4 576,476 + PGA1_c05630 0.88 +0.4 576,484 - PGA1_c05630 0.89 +0.2 576,484 - PGA1_c05630 0.89 +0.3 576,506 - -0.0 576,506 - -2.8 576,512 + +0.5 576,538 + -1.2 576,676 + PGA1_c05640 0.16 -0.8 576,714 + PGA1_c05640 0.21 -0.6 576,748 + PGA1_c05640 0.26 +0.8 576,756 - PGA1_c05640 0.28 +0.4 576,756 - PGA1_c05640 0.28 +0.3 576,865 + PGA1_c05640 0.44 +0.8 576,865 + PGA1_c05640 0.44 +0.5 576,873 - PGA1_c05640 0.45 +0.3 576,873 - PGA1_c05640 0.45 -0.6 576,873 - PGA1_c05640 0.45 -1.5 576,873 - PGA1_c05640 0.45 -1.0
Or see this region's nucleotide sequence