Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c35260 and PGA1_c35270 are separated by 334 nucleotides PGA1_c35270 and PGA1_c35280 are separated by 81 nucleotides PGA1_c35280 and PGA1_c35290 overlap by 4 nucleotides PGA1_c35290 and PGA1_c35300 are separated by 92 nucleotides PGA1_c35300 and PGA1_c35310 are separated by 62 nucleotides
PGA1_c35260: PGA1_c35260 - ATP-dependent protease HslV, at 3,697,348 to 3,697,911
_c35260
PGA1_c35270: PGA1_c35270 - Uncharacterized conserved small protein, at 3,698,246 to 3,698,440
_c35270
PGA1_c35280: PGA1_c35280 - conserved hypothetical protein, at 3,698,522 to 3,698,887
_c35280
PGA1_c35290: PGA1_c35290 - Conserved hypothetical protein (Lin0512_fam)., at 3,698,884 to 3,699,231
_c35290
PGA1_c35300: PGA1_c35300 - thioredoxin TrxA, at 3,699,324 to 3,699,644
_c35300
PGA1_c35310: PGA1_c35310 - double strand break repair helicase AddA, at 3,699,707 to 3,703,075
_c35310
Position (kb)
3698
3699
3700 Strain fitness (log2 ratio)
-2
-1
0
1 at 3697.931 kb on - strand at 3697.998 kb on - strand at 3698.076 kb on + strand at 3698.265 kb on + strand at 3698.265 kb on + strand at 3698.319 kb on + strand, within PGA1_c35270 at 3698.385 kb on + strand, within PGA1_c35270 at 3698.393 kb on - strand, within PGA1_c35270 at 3698.442 kb on - strand at 3698.442 kb on - strand at 3698.450 kb on + strand at 3698.453 kb on - strand at 3698.477 kb on + strand at 3698.509 kb on - strand at 3698.541 kb on + strand at 3698.541 kb on + strand at 3698.555 kb on - strand at 3698.572 kb on - strand, within PGA1_c35280 at 3698.630 kb on + strand, within PGA1_c35280 at 3698.683 kb on + strand, within PGA1_c35280 at 3698.748 kb on + strand, within PGA1_c35280 at 3698.754 kb on - strand, within PGA1_c35280 at 3698.754 kb on - strand, within PGA1_c35280 at 3698.754 kb on - strand, within PGA1_c35280 at 3698.795 kb on + strand, within PGA1_c35280 at 3698.803 kb on + strand, within PGA1_c35280 at 3698.803 kb on - strand, within PGA1_c35280 at 3698.817 kb on + strand, within PGA1_c35280 at 3698.817 kb on + strand, within PGA1_c35280 at 3698.817 kb on + strand, within PGA1_c35280 at 3698.822 kb on + strand, within PGA1_c35280 at 3698.823 kb on + strand, within PGA1_c35280 at 3698.823 kb on + strand, within PGA1_c35280 at 3698.823 kb on + strand, within PGA1_c35280 at 3698.826 kb on - strand, within PGA1_c35280 at 3698.830 kb on - strand, within PGA1_c35280 at 3698.831 kb on - strand, within PGA1_c35280 at 3698.831 kb on - strand, within PGA1_c35280 at 3698.937 kb on - strand, within PGA1_c35290 at 3698.938 kb on + strand, within PGA1_c35290 at 3698.974 kb on + strand, within PGA1_c35290 at 3698.975 kb on + strand, within PGA1_c35290 at 3698.975 kb on + strand, within PGA1_c35290 at 3698.975 kb on + strand, within PGA1_c35290 at 3698.983 kb on - strand, within PGA1_c35290 at 3698.983 kb on - strand, within PGA1_c35290 at 3699.007 kb on - strand, within PGA1_c35290 at 3699.015 kb on - strand, within PGA1_c35290 at 3699.020 kb on - strand, within PGA1_c35290 at 3699.049 kb on - strand, within PGA1_c35290 at 3699.073 kb on + strand, within PGA1_c35290 at 3699.081 kb on - strand, within PGA1_c35290 at 3699.127 kb on + strand, within PGA1_c35290 at 3699.175 kb on - strand, within PGA1_c35290 at 3699.175 kb on - strand, within PGA1_c35290 at 3699.197 kb on + strand at 3699.227 kb on + strand at 3699.248 kb on - strand at 3699.724 kb on + strand at 3699.747 kb on - strand at 3699.751 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 3,697,931 - +0.8 3,697,998 - -0.4 3,698,076 + +0.1 3,698,265 + +0.6 3,698,265 + +0.5 3,698,319 + PGA1_c35270 0.37 -0.5 3,698,385 + PGA1_c35270 0.71 -0.2 3,698,393 - PGA1_c35270 0.75 -0.1 3,698,442 - -0.3 3,698,442 - +0.5 3,698,450 + +0.4 3,698,453 - +1.0 3,698,477 + +0.2 3,698,509 - -0.8 3,698,541 + -0.3 3,698,541 + -0.7 3,698,555 - +0.0 3,698,572 - PGA1_c35280 0.14 -0.3 3,698,630 + PGA1_c35280 0.30 -1.2 3,698,683 + PGA1_c35280 0.44 -0.2 3,698,748 + PGA1_c35280 0.62 -0.2 3,698,754 - PGA1_c35280 0.63 -0.0 3,698,754 - PGA1_c35280 0.63 +0.6 3,698,754 - PGA1_c35280 0.63 +0.4 3,698,795 + PGA1_c35280 0.75 -0.1 3,698,803 + PGA1_c35280 0.77 -0.5 3,698,803 - PGA1_c35280 0.77 -0.1 3,698,817 + PGA1_c35280 0.81 +0.2 3,698,817 + PGA1_c35280 0.81 +0.8 3,698,817 + PGA1_c35280 0.81 +0.1 3,698,822 + PGA1_c35280 0.82 -0.2 3,698,823 + PGA1_c35280 0.82 -0.4 3,698,823 + PGA1_c35280 0.82 +0.6 3,698,823 + PGA1_c35280 0.82 +0.2 3,698,826 - PGA1_c35280 0.83 -0.4 3,698,830 - PGA1_c35280 0.84 -0.3 3,698,831 - PGA1_c35280 0.84 +0.6 3,698,831 - PGA1_c35280 0.84 -2.4 3,698,937 - PGA1_c35290 0.15 +1.5 3,698,938 + PGA1_c35290 0.16 +0.4 3,698,974 + PGA1_c35290 0.26 -0.1 3,698,975 + PGA1_c35290 0.26 +0.2 3,698,975 + PGA1_c35290 0.26 +0.6 3,698,975 + PGA1_c35290 0.26 +1.0 3,698,983 - PGA1_c35290 0.28 +0.1 3,698,983 - PGA1_c35290 0.28 +0.4 3,699,007 - PGA1_c35290 0.35 +0.5 3,699,015 - PGA1_c35290 0.38 -0.4 3,699,020 - PGA1_c35290 0.39 +0.1 3,699,049 - PGA1_c35290 0.47 +0.2 3,699,073 + PGA1_c35290 0.54 +0.6 3,699,081 - PGA1_c35290 0.57 -0.1 3,699,127 + PGA1_c35290 0.70 -0.1 3,699,175 - PGA1_c35290 0.84 +0.4 3,699,175 - PGA1_c35290 0.84 -0.2 3,699,197 + +0.8 3,699,227 + -0.3 3,699,248 - +0.0 3,699,724 + +0.2 3,699,747 - +1.1 3,699,751 - -0.5
Or see this region's nucleotide sequence