Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c12130 and PGA1_c12140 are separated by 28 nucleotides PGA1_c12140 and PGA1_c12150 overlap by 4 nucleotides PGA1_c12150 and PGA1_c12160 overlap by 4 nucleotides PGA1_c12160 and PGA1_c12170 are separated by 152 nucleotides
PGA1_c12130: PGA1_c12130 - DNA-damage-inducible protein F, at 1,259,514 to 1,260,845
_c12130
PGA1_c12140: PGA1_c12140 - putative arsenate reductase, at 1,260,874 to 1,261,215
_c12140
PGA1_c12150: PGA1_c12150 - dihydroorotate dehydrogenase PyrD, at 1,261,212 to 1,262,270
_c12150
PGA1_c12160: PGA1_c12160 - Uncharacterized protein conserved in bacteria, at 1,262,267 to 1,262,605
_c12160
PGA1_c12170: PGA1_c12170 - putative 5'-nucleotidase, at 1,262,758 to 1,264,335
_c12170
Position (kb)
1261
1262
1263 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1260.218 kb on + strand, within PGA1_c12130 at 1260.218 kb on + strand, within PGA1_c12130 at 1260.244 kb on - strand, within PGA1_c12130 at 1260.249 kb on + strand, within PGA1_c12130 at 1260.378 kb on - strand, within PGA1_c12130 at 1260.431 kb on - strand, within PGA1_c12130 at 1260.457 kb on + strand, within PGA1_c12130 at 1260.457 kb on + strand, within PGA1_c12130 at 1260.498 kb on + strand, within PGA1_c12130 at 1260.501 kb on - strand, within PGA1_c12130 at 1260.595 kb on + strand, within PGA1_c12130 at 1260.609 kb on + strand, within PGA1_c12130 at 1260.668 kb on - strand, within PGA1_c12130 at 1260.793 kb on + strand at 1260.793 kb on + strand at 1260.793 kb on + strand at 1260.799 kb on + strand at 1260.799 kb on + strand at 1260.801 kb on - strand at 1260.801 kb on - strand at 1260.807 kb on - strand at 1260.884 kb on + strand at 1260.940 kb on + strand, within PGA1_c12140 at 1260.955 kb on + strand, within PGA1_c12140 at 1261.014 kb on - strand, within PGA1_c12140 at 1261.058 kb on + strand, within PGA1_c12140 at 1261.084 kb on - strand, within PGA1_c12140 at 1261.114 kb on + strand, within PGA1_c12140 at 1261.145 kb on - strand, within PGA1_c12140 at 1261.145 kb on - strand, within PGA1_c12140 at 1261.151 kb on + strand, within PGA1_c12140 at 1261.151 kb on + strand, within PGA1_c12140 at 1261.159 kb on - strand, within PGA1_c12140 at 1261.159 kb on - strand, within PGA1_c12140 at 1261.161 kb on + strand, within PGA1_c12140 at 1261.161 kb on + strand, within PGA1_c12140 at 1261.193 kb on + strand at 1261.200 kb on + strand at 1261.329 kb on + strand, within PGA1_c12150 at 1261.329 kb on + strand, within PGA1_c12150 at 1261.329 kb on + strand, within PGA1_c12150 at 1261.337 kb on - strand, within PGA1_c12150 at 1261.337 kb on - strand, within PGA1_c12150 at 1261.339 kb on - strand, within PGA1_c12150 at 1261.386 kb on - strand, within PGA1_c12150 at 1261.391 kb on - strand, within PGA1_c12150 at 1261.391 kb on - strand, within PGA1_c12150 at 1261.489 kb on + strand, within PGA1_c12150 at 1261.525 kb on + strand, within PGA1_c12150 at 1261.535 kb on - strand, within PGA1_c12150 at 1261.535 kb on - strand, within PGA1_c12150 at 1261.535 kb on - strand, within PGA1_c12150 at 1261.535 kb on - strand, within PGA1_c12150 at 1261.546 kb on + strand, within PGA1_c12150 at 1261.658 kb on - strand, within PGA1_c12150 at 1261.681 kb on + strand, within PGA1_c12150 at 1261.693 kb on + strand, within PGA1_c12150 at 1261.693 kb on + strand, within PGA1_c12150 at 1261.701 kb on - strand, within PGA1_c12150 at 1261.715 kb on - strand, within PGA1_c12150 at 1261.717 kb on + strand, within PGA1_c12150 at 1261.717 kb on + strand, within PGA1_c12150 at 1261.725 kb on - strand, within PGA1_c12150 at 1261.747 kb on - strand, within PGA1_c12150 at 1261.839 kb on + strand, within PGA1_c12150 at 1261.839 kb on + strand, within PGA1_c12150 at 1261.989 kb on - strand, within PGA1_c12150 at 1261.989 kb on - strand, within PGA1_c12150 at 1262.024 kb on + strand, within PGA1_c12150 at 1262.085 kb on + strand, within PGA1_c12150 at 1262.114 kb on + strand, within PGA1_c12150 at 1262.144 kb on + strand, within PGA1_c12150 at 1262.240 kb on - strand at 1262.255 kb on - strand at 1262.264 kb on + strand at 1262.264 kb on + strand at 1262.322 kb on + strand, within PGA1_c12160 at 1262.324 kb on - strand, within PGA1_c12160 at 1262.330 kb on - strand, within PGA1_c12160 at 1262.401 kb on + strand, within PGA1_c12160 at 1262.404 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.437 kb on + strand, within PGA1_c12160 at 1262.437 kb on + strand, within PGA1_c12160 at 1262.612 kb on + strand at 1262.612 kb on + strand at 1262.612 kb on + strand at 1262.620 kb on - strand at 1262.620 kb on - strand at 1262.620 kb on - strand at 1262.625 kb on + strand at 1262.668 kb on + strand at 1262.721 kb on + strand at 1262.745 kb on + strand at 1262.797 kb on - strand at 1262.859 kb on + strand at 1262.902 kb on - strand at 1262.922 kb on - strand, within PGA1_c12170 at 1262.927 kb on - strand, within PGA1_c12170 at 1262.946 kb on + strand, within PGA1_c12170 at 1262.993 kb on - strand, within PGA1_c12170 at 1263.014 kb on + strand, within PGA1_c12170 at 1263.030 kb on + strand, within PGA1_c12170 at 1263.030 kb on + strand, within PGA1_c12170 at 1263.032 kb on + strand, within PGA1_c12170 at 1263.038 kb on - strand, within PGA1_c12170 at 1263.200 kb on + strand, within PGA1_c12170
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 1,260,218 + PGA1_c12130 0.53 +0.2 1,260,218 + PGA1_c12130 0.53 -1.1 1,260,244 - PGA1_c12130 0.55 +0.3 1,260,249 + PGA1_c12130 0.55 +1.7 1,260,378 - PGA1_c12130 0.65 +0.5 1,260,431 - PGA1_c12130 0.69 +0.4 1,260,457 + PGA1_c12130 0.71 +0.4 1,260,457 + PGA1_c12130 0.71 -0.3 1,260,498 + PGA1_c12130 0.74 -0.6 1,260,501 - PGA1_c12130 0.74 -2.0 1,260,595 + PGA1_c12130 0.81 +0.7 1,260,609 + PGA1_c12130 0.82 -0.5 1,260,668 - PGA1_c12130 0.87 -0.8 1,260,793 + -0.1 1,260,793 + -0.1 1,260,793 + +0.2 1,260,799 + -0.3 1,260,799 + +0.4 1,260,801 - -0.9 1,260,801 - -0.5 1,260,807 - -0.2 1,260,884 + +0.4 1,260,940 + PGA1_c12140 0.19 -0.2 1,260,955 + PGA1_c12140 0.24 -0.2 1,261,014 - PGA1_c12140 0.41 +1.1 1,261,058 + PGA1_c12140 0.54 -1.4 1,261,084 - PGA1_c12140 0.61 -0.3 1,261,114 + PGA1_c12140 0.70 -0.4 1,261,145 - PGA1_c12140 0.79 +0.4 1,261,145 - PGA1_c12140 0.79 +0.5 1,261,151 + PGA1_c12140 0.81 -0.1 1,261,151 + PGA1_c12140 0.81 +0.5 1,261,159 - PGA1_c12140 0.83 +0.1 1,261,159 - PGA1_c12140 0.83 +0.2 1,261,161 + PGA1_c12140 0.84 +0.6 1,261,161 + PGA1_c12140 0.84 -1.3 1,261,193 + +0.3 1,261,200 + +1.0 1,261,329 + PGA1_c12150 0.11 -2.9 1,261,329 + PGA1_c12150 0.11 -3.9 1,261,329 + PGA1_c12150 0.11 -3.2 1,261,337 - PGA1_c12150 0.12 -3.0 1,261,337 - PGA1_c12150 0.12 -2.5 1,261,339 - PGA1_c12150 0.12 -1.8 1,261,386 - PGA1_c12150 0.16 -4.0 1,261,391 - PGA1_c12150 0.17 -3.6 1,261,391 - PGA1_c12150 0.17 -3.2 1,261,489 + PGA1_c12150 0.26 -3.1 1,261,525 + PGA1_c12150 0.30 -2.2 1,261,535 - PGA1_c12150 0.31 -3.4 1,261,535 - PGA1_c12150 0.31 -4.4 1,261,535 - PGA1_c12150 0.31 -1.9 1,261,535 - PGA1_c12150 0.31 -2.0 1,261,546 + PGA1_c12150 0.32 -3.6 1,261,658 - PGA1_c12150 0.42 -3.2 1,261,681 + PGA1_c12150 0.44 -3.2 1,261,693 + PGA1_c12150 0.45 -3.4 1,261,693 + PGA1_c12150 0.45 -3.3 1,261,701 - PGA1_c12150 0.46 -1.8 1,261,715 - PGA1_c12150 0.47 -3.6 1,261,717 + PGA1_c12150 0.48 -3.5 1,261,717 + PGA1_c12150 0.48 -3.1 1,261,725 - PGA1_c12150 0.48 -4.1 1,261,747 - PGA1_c12150 0.51 -3.5 1,261,839 + PGA1_c12150 0.59 -3.0 1,261,839 + PGA1_c12150 0.59 -2.7 1,261,989 - PGA1_c12150 0.73 -3.3 1,261,989 - PGA1_c12150 0.73 -2.3 1,262,024 + PGA1_c12150 0.77 -1.9 1,262,085 + PGA1_c12150 0.82 -4.3 1,262,114 + PGA1_c12150 0.85 -3.6 1,262,144 + PGA1_c12150 0.88 -1.5 1,262,240 - -2.5 1,262,255 - -1.2 1,262,264 + -3.8 1,262,264 + -1.7 1,262,322 + PGA1_c12160 0.16 -1.2 1,262,324 - PGA1_c12160 0.17 -0.5 1,262,330 - PGA1_c12160 0.19 -2.4 1,262,401 + PGA1_c12160 0.40 -2.2 1,262,404 - PGA1_c12160 0.40 -2.2 1,262,409 - PGA1_c12160 0.42 -1.8 1,262,409 - PGA1_c12160 0.42 -1.7 1,262,409 - PGA1_c12160 0.42 -3.0 1,262,409 - PGA1_c12160 0.42 -2.3 1,262,409 - PGA1_c12160 0.42 -1.3 1,262,437 + PGA1_c12160 0.50 -1.8 1,262,437 + PGA1_c12160 0.50 -2.3 1,262,612 + -2.4 1,262,612 + -0.9 1,262,612 + -0.3 1,262,620 - -2.4 1,262,620 - -2.0 1,262,620 - -0.9 1,262,625 + -0.8 1,262,668 + +0.4 1,262,721 + -0.6 1,262,745 + -1.3 1,262,797 - +0.3 1,262,859 + -0.0 1,262,902 - -0.0 1,262,922 - PGA1_c12170 0.10 -0.1 1,262,927 - PGA1_c12170 0.11 +0.5 1,262,946 + PGA1_c12170 0.12 +1.2 1,262,993 - PGA1_c12170 0.15 +0.1 1,263,014 + PGA1_c12170 0.16 +0.6 1,263,030 + PGA1_c12170 0.17 -0.3 1,263,030 + PGA1_c12170 0.17 -1.4 1,263,032 + PGA1_c12170 0.17 -1.6 1,263,038 - PGA1_c12170 0.18 +0.7 1,263,200 + PGA1_c12170 0.28 -0.3
Or see this region's nucleotide sequence