Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_96

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_95 and Pf1N1B4_96 are separated by 158 nucleotidesPf1N1B4_96 and Pf1N1B4_97 overlap by 1 nucleotides Pf1N1B4_95 - contains type I hydrophobic transmembrane region and ATP/GTP binding motif, at 100,013 to 100,699 _95 Pf1N1B4_96 - Selenophosphate-dependent tRNA 2-selenouridine synthase, at 100,858 to 101,961 _96 Pf1N1B4_97 - Selenide,water dikinase (EC 2.7.9.3), at 101,961 to 102,995 _97 Position (kb) 100 101 102Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 100.004 kb on - strandat 100.019 kb on + strandat 100.020 kb on - strandat 100.020 kb on - strandat 100.071 kb on - strandat 100.071 kb on - strandat 100.071 kb on - strandat 100.071 kb on - strandat 100.071 kb on - strandat 100.071 kb on - strandat 100.072 kb on + strandat 100.073 kb on - strandat 100.073 kb on - strandat 100.286 kb on + strand, within Pf1N1B4_95at 100.286 kb on + strand, within Pf1N1B4_95at 100.286 kb on + strand, within Pf1N1B4_95at 100.287 kb on - strand, within Pf1N1B4_95at 100.337 kb on - strand, within Pf1N1B4_95at 100.337 kb on - strand, within Pf1N1B4_95at 100.343 kb on + strand, within Pf1N1B4_95at 100.344 kb on - strand, within Pf1N1B4_95at 100.349 kb on + strand, within Pf1N1B4_95at 100.349 kb on + strand, within Pf1N1B4_95at 100.350 kb on - strand, within Pf1N1B4_95at 100.350 kb on - strandat 100.467 kb on - strand, within Pf1N1B4_95at 100.467 kb on - strand, within Pf1N1B4_95at 100.484 kb on + strand, within Pf1N1B4_95at 100.484 kb on + strand, within Pf1N1B4_95at 100.485 kb on - strand, within Pf1N1B4_95at 100.602 kb on - strand, within Pf1N1B4_95at 100.602 kb on - strand, within Pf1N1B4_95at 100.655 kb on + strandat 100.655 kb on + strandat 100.656 kb on - strandat 100.656 kb on - strandat 100.656 kb on - strandat 100.697 kb on + strandat 100.697 kb on + strandat 100.698 kb on - strandat 100.726 kb on + strandat 100.726 kb on + strandat 100.728 kb on + strandat 100.728 kb on + strandat 100.729 kb on - strandat 100.890 kb on - strandat 100.890 kb on - strandat 100.959 kb on - strandat 100.959 kb on - strandat 100.971 kb on - strand, within Pf1N1B4_96at 100.971 kb on - strand, within Pf1N1B4_96at 100.973 kb on + strand, within Pf1N1B4_96at 101.160 kb on - strand, within Pf1N1B4_96at 101.195 kb on + strand, within Pf1N1B4_96at 101.196 kb on - strand, within Pf1N1B4_96at 101.341 kb on - strand, within Pf1N1B4_96at 101.682 kb on - strand, within Pf1N1B4_96at 101.940 kb on - strandat 102.157 kb on + strand, within Pf1N1B4_97at 102.157 kb on + strand, within Pf1N1B4_97at 102.157 kb on + strand, within Pf1N1B4_97at 102.157 kb on + strand, within Pf1N1B4_97at 102.157 kb on + strand, within Pf1N1B4_97at 102.158 kb on - strand, within Pf1N1B4_97at 102.158 kb on - strand, within Pf1N1B4_97at 102.158 kb on - strand, within Pf1N1B4_97at 102.158 kb on - strand, within Pf1N1B4_97at 102.209 kb on - strand, within Pf1N1B4_97at 102.211 kb on + strand, within Pf1N1B4_97at 102.211 kb on + strand, within Pf1N1B4_97at 102.211 kb on + strand, within Pf1N1B4_97at 102.211 kb on + strand, within Pf1N1B4_97at 102.212 kb on - strandat 102.212 kb on - strand, within Pf1N1B4_97at 102.212 kb on - strand, within Pf1N1B4_97at 102.212 kb on - strand, within Pf1N1B4_97at 102.212 kb on - strandat 102.212 kb on - strand, within Pf1N1B4_97at 102.475 kb on + strand, within Pf1N1B4_97at 102.475 kb on + strand, within Pf1N1B4_97at 102.476 kb on - strand, within Pf1N1B4_97at 102.476 kb on - strand, within Pf1N1B4_97at 102.722 kb on - strand, within Pf1N1B4_97at 102.722 kb on - strand, within Pf1N1B4_97at 102.722 kb on - strand, within Pf1N1B4_97at 102.835 kb on + strand, within Pf1N1B4_97at 102.836 kb on - strand, within Pf1N1B4_97at 102.836 kb on - strand, within Pf1N1B4_97at 102.836 kb on - strand, within Pf1N1B4_97at 102.836 kb on - strand, within Pf1N1B4_97

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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100,004 - +0.0
100,019 + -1.3
100,020 - -1.8
100,020 - +0.0
100,071 - -3.5
100,071 - +0.0
100,071 - -1.6
100,071 - +0.0
100,071 - -1.8
100,071 - -1.0
100,072 + +0.0
100,073 - +0.0
100,073 - +0.0
100,286 + Pf1N1B4_95 0.40 -4.2
100,286 + Pf1N1B4_95 0.40 +0.0
100,286 + Pf1N1B4_95 0.40 -2.6
100,287 - Pf1N1B4_95 0.40 +0.0
100,337 - Pf1N1B4_95 0.47 +0.0
100,337 - Pf1N1B4_95 0.47 -1.0
100,343 + Pf1N1B4_95 0.48 -0.8
100,344 - Pf1N1B4_95 0.48 +0.0
100,349 + Pf1N1B4_95 0.49 -1.5
100,349 + Pf1N1B4_95 0.49 +0.0
100,350 - Pf1N1B4_95 0.49 -3.8
100,350 - +0.0
100,467 - Pf1N1B4_95 0.66 -3.9
100,467 - Pf1N1B4_95 0.66 +0.0
100,484 + Pf1N1B4_95 0.69 +0.0
100,484 + Pf1N1B4_95 0.69 -4.2
100,485 - Pf1N1B4_95 0.69 -0.9
100,602 - Pf1N1B4_95 0.86 -2.2
100,602 - Pf1N1B4_95 0.86 +0.0
100,655 + +0.0
100,655 + -1.0
100,656 - +0.0
100,656 - +0.0
100,656 - -1.5
100,697 + -2.0
100,697 + +0.0
100,698 - -1.6
100,726 + -2.4
100,726 + +0.0
100,728 + +0.0
100,728 + +0.0
100,729 - -2.8
100,890 - +0.0
100,890 - -1.0
100,959 - +0.0
100,959 - -2.0
100,971 - Pf1N1B4_96 0.10 -1.7
100,971 - Pf1N1B4_96 0.10 +0.0
100,973 + Pf1N1B4_96 0.10 -2.1
101,160 - Pf1N1B4_96 0.27 +0.0
101,195 + Pf1N1B4_96 0.31 -1.7
101,196 - Pf1N1B4_96 0.31 -2.2
101,341 - Pf1N1B4_96 0.44 +0.0
101,682 - Pf1N1B4_96 0.75 -2.2
101,940 - -4.0
102,157 + Pf1N1B4_97 0.19 +0.0
102,157 + Pf1N1B4_97 0.19 +0.0
102,157 + Pf1N1B4_97 0.19 -3.1
102,157 + Pf1N1B4_97 0.19 +0.0
102,157 + Pf1N1B4_97 0.19 -2.2
102,158 - Pf1N1B4_97 0.19 +0.0
102,158 - Pf1N1B4_97 0.19 +0.0
102,158 - Pf1N1B4_97 0.19 -4.0
102,158 - Pf1N1B4_97 0.19 +0.0
102,209 - Pf1N1B4_97 0.24 -3.1
102,211 + Pf1N1B4_97 0.24 +0.0
102,211 + Pf1N1B4_97 0.24 +0.0
102,211 + Pf1N1B4_97 0.24 +0.0
102,211 + Pf1N1B4_97 0.24 -2.2
102,212 - +0.0
102,212 - Pf1N1B4_97 0.24 -3.6
102,212 - Pf1N1B4_97 0.24 +0.0
102,212 - Pf1N1B4_97 0.24 +0.0
102,212 - -3.0
102,212 - Pf1N1B4_97 0.24 +0.0
102,475 + Pf1N1B4_97 0.50 +0.0
102,475 + Pf1N1B4_97 0.50 -2.6
102,476 - Pf1N1B4_97 0.50 -3.8
102,476 - Pf1N1B4_97 0.50 +0.0
102,722 - Pf1N1B4_97 0.74 +0.0
102,722 - Pf1N1B4_97 0.74 +0.0
102,722 - Pf1N1B4_97 0.74 +0.0
102,835 + Pf1N1B4_97 0.84 -5.2
102,836 - Pf1N1B4_97 0.85 +0.0
102,836 - Pf1N1B4_97 0.85 +0.0
102,836 - Pf1N1B4_97 0.85 +0.0
102,836 - Pf1N1B4_97 0.85 -2.3

Or see this region's nucleotide sequence