Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_897 and Pf1N1B4_898 are separated by 295 nucleotides Pf1N1B4_898 and Pf1N1B4_899 are separated by 326 nucleotides Pf1N1B4_899 and Pf1N1B4_900 are separated by 8 nucleotides
Pf1N1B4_897 - FIG000988: Predicted permease, at 1,023,355 to 1,024,473
_897
Pf1N1B4_898 - Cytosol aminopeptidase PepA (EC 3.4.11.1), at 1,024,769 to 1,026,259
_898
Pf1N1B4_899 - DNA polymerase III chi subunit (EC 2.7.7.7), at 1,026,586 to 1,027,014
_899
Pf1N1B4_900 - FIG00957284: hypothetical protein, at 1,027,023 to 1,027,412
_900
Position (kb) 1024
1025
1026
1027 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1024.522 kb on - strand at 1024.710 kb on + strand at 1024.710 kb on + strand at 1024.710 kb on + strand at 1024.710 kb on + strand at 1024.710 kb on + strand at 1024.711 kb on - strand at 1024.711 kb on - strand at 1024.711 kb on - strand at 1024.714 kb on + strand at 1024.714 kb on + strand at 1024.714 kb on + strand at 1024.714 kb on + strand at 1024.714 kb on + strand at 1024.714 kb on + strand at 1024.714 kb on + strand at 1024.714 kb on + strand at 1024.715 kb on - strand at 1024.715 kb on - strand at 1024.715 kb on - strand at 1024.715 kb on - strand at 1024.715 kb on - strand at 1024.715 kb on - strand at 1024.715 kb on - strand at 1024.716 kb on + strand at 1024.716 kb on + strand at 1024.716 kb on + strand at 1024.716 kb on + strand at 1024.716 kb on + strand at 1024.716 kb on + strand at 1024.716 kb on + strand at 1024.716 kb on + strand at 1024.716 kb on + strand at 1024.717 kb on - strand at 1024.717 kb on - strand at 1024.717 kb on - strand at 1024.717 kb on - strand at 1024.717 kb on - strand at 1024.717 kb on - strand at 1024.717 kb on - strand at 1024.717 kb on - strand at 1024.717 kb on - strand at 1024.752 kb on - strand at 1024.782 kb on + strand at 1024.783 kb on - strand at 1024.783 kb on - strand at 1025.115 kb on + strand, within Pf1N1B4_898 at 1025.136 kb on - strand, within Pf1N1B4_898 at 1025.136 kb on - strand, within Pf1N1B4_898 at 1025.169 kb on + strand, within Pf1N1B4_898 at 1025.169 kb on + strand, within Pf1N1B4_898 at 1025.169 kb on + strand, within Pf1N1B4_898 at 1025.170 kb on - strand, within Pf1N1B4_898 at 1025.170 kb on - strand, within Pf1N1B4_898 at 1025.170 kb on - strand, within Pf1N1B4_898 at 1025.170 kb on - strand, within Pf1N1B4_898 at 1025.170 kb on - strand, within Pf1N1B4_898 at 1025.180 kb on + strand, within Pf1N1B4_898 at 1025.181 kb on - strand, within Pf1N1B4_898 at 1025.181 kb on - strand, within Pf1N1B4_898 at 1025.251 kb on + strand, within Pf1N1B4_898 at 1025.252 kb on - strand, within Pf1N1B4_898 at 1025.468 kb on + strand, within Pf1N1B4_898 at 1025.468 kb on + strand, within Pf1N1B4_898 at 1025.469 kb on - strand, within Pf1N1B4_898 at 1025.519 kb on + strand, within Pf1N1B4_898 at 1025.519 kb on + strand, within Pf1N1B4_898 at 1025.519 kb on + strand, within Pf1N1B4_898 at 1025.520 kb on - strand, within Pf1N1B4_898 at 1025.520 kb on - strand, within Pf1N1B4_898 at 1025.520 kb on - strand, within Pf1N1B4_898 at 1025.520 kb on - strand, within Pf1N1B4_898 at 1025.630 kb on + strand at 1025.630 kb on + strand, within Pf1N1B4_898 at 1025.630 kb on + strand, within Pf1N1B4_898 at 1025.630 kb on + strand, within Pf1N1B4_898 at 1025.630 kb on + strand, within Pf1N1B4_898 at 1025.630 kb on + strand, within Pf1N1B4_898 at 1025.631 kb on - strand, within Pf1N1B4_898 at 1025.631 kb on - strand, within Pf1N1B4_898 at 1025.849 kb on + strand, within Pf1N1B4_898 at 1025.849 kb on + strand, within Pf1N1B4_898 at 1025.849 kb on + strand, within Pf1N1B4_898 at 1026.033 kb on - strand, within Pf1N1B4_898 at 1026.033 kb on - strand, within Pf1N1B4_898 at 1026.033 kb on - strand, within Pf1N1B4_898 at 1026.233 kb on + strand at 1026.234 kb on - strand at 1026.234 kb on - strand at 1026.286 kb on + strand at 1026.286 kb on + strand at 1026.286 kb on + strand at 1026.286 kb on + strand at 1026.286 kb on + strand at 1026.496 kb on - strand at 1026.520 kb on + strand at 1026.520 kb on + strand at 1026.520 kb on + strand at 1026.521 kb on - strand at 1026.604 kb on + strand at 1026.604 kb on + strand at 1026.604 kb on + strand at 1026.604 kb on + strand at 1026.604 kb on + strand at 1026.604 kb on + strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.607 kb on - strand at 1026.691 kb on + strand, within Pf1N1B4_899 at 1026.691 kb on + strand, within Pf1N1B4_899 at 1026.702 kb on + strand at 1026.879 kb on - strand, within Pf1N1B4_899 at 1027.001 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 1,024,522 - -0.0 1,024,710 + -2.7 1,024,710 + -0.0 1,024,710 + -0.0 1,024,710 + -0.0 1,024,710 + -0.0 1,024,711 - -0.0 1,024,711 - -0.0 1,024,711 - -3.8 1,024,714 + -0.0 1,024,714 + -0.0 1,024,714 + -0.0 1,024,714 + -0.0 1,024,714 + -0.0 1,024,714 + -0.0 1,024,714 + -2.0 1,024,714 + -0.0 1,024,715 - -1.5 1,024,715 - -0.0 1,024,715 - -0.0 1,024,715 - -0.0 1,024,715 - -0.0 1,024,715 - -0.0 1,024,715 - -0.0 1,024,716 + -0.0 1,024,716 + -3.0 1,024,716 + -0.0 1,024,716 + -0.0 1,024,716 + -0.0 1,024,716 + -5.1 1,024,716 + -3.7 1,024,716 + -0.0 1,024,716 + -2.2 1,024,717 - -0.0 1,024,717 - -0.0 1,024,717 - -0.0 1,024,717 - -0.0 1,024,717 - -0.0 1,024,717 - -1.8 1,024,717 - -0.0 1,024,717 - -0.0 1,024,717 - -2.6 1,024,752 - -1.0 1,024,782 + -0.0 1,024,783 - -1.3 1,024,783 - -0.0 1,025,115 + Pf1N1B4_898 0.23 -0.0 1,025,136 - Pf1N1B4_898 0.25 -0.4 1,025,136 - Pf1N1B4_898 0.25 -0.0 1,025,169 + Pf1N1B4_898 0.27 -0.0 1,025,169 + Pf1N1B4_898 0.27 -0.0 1,025,169 + Pf1N1B4_898 0.27 -1.2 1,025,170 - Pf1N1B4_898 0.27 -0.0 1,025,170 - Pf1N1B4_898 0.27 -0.0 1,025,170 - Pf1N1B4_898 0.27 -0.8 1,025,170 - Pf1N1B4_898 0.27 -0.0 1,025,170 - Pf1N1B4_898 0.27 -0.8 1,025,180 + Pf1N1B4_898 0.28 -0.6 1,025,181 - Pf1N1B4_898 0.28 -0.0 1,025,181 - Pf1N1B4_898 0.28 -0.0 1,025,251 + Pf1N1B4_898 0.32 -0.0 1,025,252 - Pf1N1B4_898 0.32 -1.9 1,025,468 + Pf1N1B4_898 0.47 -1.9 1,025,468 + Pf1N1B4_898 0.47 -0.0 1,025,469 - Pf1N1B4_898 0.47 +0.8 1,025,519 + Pf1N1B4_898 0.50 -0.0 1,025,519 + Pf1N1B4_898 0.50 -0.0 1,025,519 + Pf1N1B4_898 0.50 -0.0 1,025,520 - Pf1N1B4_898 0.50 -0.0 1,025,520 - Pf1N1B4_898 0.50 -1.2 1,025,520 - Pf1N1B4_898 0.50 -0.0 1,025,520 - Pf1N1B4_898 0.50 -0.0 1,025,630 + -1.7 1,025,630 + Pf1N1B4_898 0.58 -0.0 1,025,630 + Pf1N1B4_898 0.58 -0.0 1,025,630 + Pf1N1B4_898 0.58 -0.0 1,025,630 + Pf1N1B4_898 0.58 -0.0 1,025,630 + Pf1N1B4_898 0.58 -0.2 1,025,631 - Pf1N1B4_898 0.58 -0.0 1,025,631 - Pf1N1B4_898 0.58 -2.1 1,025,849 + Pf1N1B4_898 0.72 -0.0 1,025,849 + Pf1N1B4_898 0.72 -0.0 1,025,849 + Pf1N1B4_898 0.72 -1.2 1,026,033 - Pf1N1B4_898 0.85 -0.0 1,026,033 - Pf1N1B4_898 0.85 -0.0 1,026,033 - Pf1N1B4_898 0.85 -0.0 1,026,233 + -0.4 1,026,234 - +1.6 1,026,234 - -0.0 1,026,286 + -0.1 1,026,286 + -1.7 1,026,286 + -0.0 1,026,286 + -0.0 1,026,286 + -0.0 1,026,496 - -0.0 1,026,520 + -3.0 1,026,520 + -0.0 1,026,520 + -0.0 1,026,521 - -3.8 1,026,604 + -0.0 1,026,604 + -0.0 1,026,604 + -0.0 1,026,604 + -2.4 1,026,604 + -0.0 1,026,604 + -3.2 1,026,605 - -0.0 1,026,605 - -0.0 1,026,605 - -0.0 1,026,605 - -0.0 1,026,605 - -0.0 1,026,605 - -2.2 1,026,605 - -0.0 1,026,605 - -0.0 1,026,605 - -0.0 1,026,605 - -3.3 1,026,605 - -0.0 1,026,605 - -0.0 1,026,607 - -0.9 1,026,691 + Pf1N1B4_899 0.24 -1.4 1,026,691 + Pf1N1B4_899 0.24 -0.0 1,026,702 + -1.8 1,026,879 - Pf1N1B4_899 0.68 -2.1 1,027,001 + -0.0
Or see this region's nucleotide sequence