Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_846

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_845 and Pf1N1B4_846 are separated by 232 nucleotidesPf1N1B4_846 and Pf1N1B4_847 are separated by 15 nucleotides Pf1N1B4_845 - Membrane proteins related to metalloendopeptidases, at 973,712 to 974,533 _845 Pf1N1B4_846 - hypothetical protein, at 974,766 to 974,969 _846 Pf1N1B4_847 - 2-isopropylmalate synthase (EC 2.3.3.13), at 974,985 to 976,664 _847 Position (kb) 974 975Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 973.775 kb on + strandat 973.775 kb on + strandat 973.775 kb on + strandat 973.775 kb on + strandat 973.775 kb on + strandat 973.775 kb on + strandat 973.775 kb on + strandat 973.775 kb on + strandat 973.775 kb on + strandat 973.775 kb on + strandat 973.775 kb on + strandat 973.775 kb on + strandat 973.775 kb on + strandat 973.776 kb on - strandat 973.776 kb on - strandat 973.776 kb on - strandat 973.776 kb on - strandat 973.776 kb on - strandat 973.776 kb on - strandat 973.776 kb on - strandat 973.776 kb on - strandat 973.776 kb on - strandat 973.776 kb on - strandat 973.862 kb on + strand, within Pf1N1B4_845at 973.863 kb on - strand, within Pf1N1B4_845at 973.863 kb on - strand, within Pf1N1B4_845at 973.935 kb on + strand, within Pf1N1B4_845at 973.935 kb on + strandat 974.003 kb on + strand, within Pf1N1B4_845at 974.215 kb on + strand, within Pf1N1B4_845at 974.215 kb on + strand, within Pf1N1B4_845at 974.215 kb on + strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.304 kb on - strand, within Pf1N1B4_845at 974.304 kb on - strand, within Pf1N1B4_845at 974.304 kb on - strand, within Pf1N1B4_845at 974.368 kb on + strand, within Pf1N1B4_845at 974.368 kb on + strand, within Pf1N1B4_845at 974.368 kb on + strand, within Pf1N1B4_845at 974.369 kb on - strand, within Pf1N1B4_845at 974.369 kb on - strand, within Pf1N1B4_845at 974.461 kb on + strandat 974.461 kb on + strandat 974.462 kb on - strandat 974.462 kb on - strandat 974.656 kb on - strandat 974.656 kb on - strandat 974.729 kb on + strandat 974.915 kb on + strand, within Pf1N1B4_846at 974.915 kb on + strandat 974.916 kb on - strandat 974.922 kb on + strand, within Pf1N1B4_846at 974.972 kb on + strandat 974.972 kb on + strandat 975.016 kb on - strandat 975.016 kb on - strandat 975.246 kb on - strand, within Pf1N1B4_847at 975.246 kb on - strand, within Pf1N1B4_847at 975.326 kb on + strand, within Pf1N1B4_847at 975.327 kb on - strand, within Pf1N1B4_847at 975.327 kb on - strandat 975.327 kb on - strand, within Pf1N1B4_847at 975.372 kb on + strand, within Pf1N1B4_847at 975.372 kb on + strand, within Pf1N1B4_847at 975.373 kb on - strand, within Pf1N1B4_847at 975.373 kb on - strand, within Pf1N1B4_847at 975.373 kb on - strandat 975.654 kb on + strand, within Pf1N1B4_847at 975.654 kb on + strand, within Pf1N1B4_847at 975.654 kb on + strand, within Pf1N1B4_847at 975.654 kb on + strand, within Pf1N1B4_847at 975.655 kb on - strand, within Pf1N1B4_847at 975.655 kb on - strand, within Pf1N1B4_847at 975.655 kb on - strand, within Pf1N1B4_847at 975.655 kb on - strand, within Pf1N1B4_847at 975.655 kb on - strand, within Pf1N1B4_847at 975.655 kb on - strandat 975.861 kb on + strand, within Pf1N1B4_847at 975.861 kb on + strand, within Pf1N1B4_847at 975.862 kb on - strand, within Pf1N1B4_847at 975.862 kb on - strand, within Pf1N1B4_847at 975.862 kb on - strandat 975.862 kb on - strand, within Pf1N1B4_847at 975.963 kb on + strand, within Pf1N1B4_847

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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973,775 + -0.0
973,775 + -2.2
973,775 + -0.0
973,775 + -0.0
973,775 + -0.0
973,775 + -0.0
973,775 + -0.0
973,775 + -0.0
973,775 + -0.0
973,775 + -1.0
973,775 + -0.0
973,775 + -3.1
973,775 + -0.0
973,776 - -0.0
973,776 - -2.2
973,776 - -0.0
973,776 - -0.0
973,776 - -0.0
973,776 - -1.9
973,776 - -2.1
973,776 - -1.8
973,776 - -0.0
973,776 - -1.7
973,862 + Pf1N1B4_845 0.18 -0.0
973,863 - Pf1N1B4_845 0.18 -0.3
973,863 - Pf1N1B4_845 0.18 -0.0
973,935 + Pf1N1B4_845 0.27 -1.3
973,935 + -2.5
974,003 + Pf1N1B4_845 0.35 -0.0
974,215 + Pf1N1B4_845 0.61 -4.1
974,215 + Pf1N1B4_845 0.61 -0.0
974,215 + Pf1N1B4_845 0.61 -0.0
974,216 - Pf1N1B4_845 0.61 -0.0
974,216 - Pf1N1B4_845 0.61 +1.0
974,216 - Pf1N1B4_845 0.61 -2.0
974,216 - Pf1N1B4_845 0.61 -0.0
974,216 - Pf1N1B4_845 0.61 -1.1
974,216 - Pf1N1B4_845 0.61 -0.0
974,304 - Pf1N1B4_845 0.72 -2.5
974,304 - Pf1N1B4_845 0.72 -0.0
974,304 - Pf1N1B4_845 0.72 -1.7
974,368 + Pf1N1B4_845 0.80 -0.0
974,368 + Pf1N1B4_845 0.80 -0.0
974,368 + Pf1N1B4_845 0.80 -1.7
974,369 - Pf1N1B4_845 0.80 -0.0
974,369 - Pf1N1B4_845 0.80 -0.0
974,461 + -0.0
974,461 + -0.0
974,462 - -0.0
974,462 - -0.0
974,656 - -0.5
974,656 - -0.0
974,729 + -0.0
974,915 + Pf1N1B4_846 0.73 -3.1
974,915 + -0.0
974,916 - -4.5
974,922 + Pf1N1B4_846 0.76 -2.4
974,972 + -0.0
974,972 + -5.9
975,016 - -5.0
975,016 - -0.0
975,246 - Pf1N1B4_847 0.16 -0.0
975,246 - Pf1N1B4_847 0.16 -5.2
975,326 + Pf1N1B4_847 0.20 -0.0
975,327 - Pf1N1B4_847 0.20 -0.0
975,327 - -0.0
975,327 - Pf1N1B4_847 0.20 -0.0
975,372 + Pf1N1B4_847 0.23 -0.0
975,372 + Pf1N1B4_847 0.23 -0.0
975,373 - Pf1N1B4_847 0.23 -0.0
975,373 - Pf1N1B4_847 0.23 -4.4
975,373 - -5.2
975,654 + Pf1N1B4_847 0.40 -0.0
975,654 + Pf1N1B4_847 0.40 -3.4
975,654 + Pf1N1B4_847 0.40 -0.0
975,654 + Pf1N1B4_847 0.40 -0.0
975,655 - Pf1N1B4_847 0.40 -4.7
975,655 - Pf1N1B4_847 0.40 -0.0
975,655 - Pf1N1B4_847 0.40 -5.3
975,655 - Pf1N1B4_847 0.40 -0.0
975,655 - Pf1N1B4_847 0.40 -0.0
975,655 - -5.1
975,861 + Pf1N1B4_847 0.52 -0.0
975,861 + Pf1N1B4_847 0.52 -0.0
975,862 - Pf1N1B4_847 0.52 -0.0
975,862 - Pf1N1B4_847 0.52 -0.0
975,862 - -0.0
975,862 - Pf1N1B4_847 0.52 -5.6
975,963 + Pf1N1B4_847 0.58 -0.0

Or see this region's nucleotide sequence