Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_784

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_783 and Pf1N1B4_784 are separated by 108 nucleotidesPf1N1B4_784 and Pf1N1B4_785 are separated by 282 nucleotidesPf1N1B4_785 and Pf1N1B4_786 are separated by 1 nucleotides Pf1N1B4_783 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (EC 2.1.1.14), at 892,441 to 894,753 _783 Pf1N1B4_784 - Transcriptional activator MetR, at 894,862 to 895,779 _784 Pf1N1B4_785 - acetyltransferase, GNAT family, at 896,062 to 896,577 _785 Pf1N1B4_786 - L-lysine permease, at 896,579 to 897,217 _786 Position (kb) 894 895 896Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 893.879 kb on + strand, within Pf1N1B4_783at 893.999 kb on + strand, within Pf1N1B4_783at 894.000 kb on - strand, within Pf1N1B4_783at 894.029 kb on + strand, within Pf1N1B4_783at 894.044 kb on + strand, within Pf1N1B4_783at 894.045 kb on - strand, within Pf1N1B4_783at 894.230 kb on + strand, within Pf1N1B4_783at 894.230 kb on + strand, within Pf1N1B4_783at 894.230 kb on + strand, within Pf1N1B4_783at 894.231 kb on - strand, within Pf1N1B4_783at 894.231 kb on - strand, within Pf1N1B4_783at 894.231 kb on - strand, within Pf1N1B4_783at 894.231 kb on - strand, within Pf1N1B4_783at 894.357 kb on - strand, within Pf1N1B4_783at 894.357 kb on - strand, within Pf1N1B4_783at 894.362 kb on + strand, within Pf1N1B4_783at 894.362 kb on + strand, within Pf1N1B4_783at 894.362 kb on + strand, within Pf1N1B4_783at 894.362 kb on + strand, within Pf1N1B4_783at 894.363 kb on - strand, within Pf1N1B4_783at 894.363 kb on - strandat 894.363 kb on - strand, within Pf1N1B4_783at 894.366 kb on + strand, within Pf1N1B4_783at 894.366 kb on + strand, within Pf1N1B4_783at 894.366 kb on + strand, within Pf1N1B4_783at 894.411 kb on + strand, within Pf1N1B4_783at 894.411 kb on + strand, within Pf1N1B4_783at 894.412 kb on - strand, within Pf1N1B4_783at 894.412 kb on - strand, within Pf1N1B4_783at 894.547 kb on + strandat 894.547 kb on + strandat 894.770 kb on - strandat 894.771 kb on + strandat 894.772 kb on - strandat 894.784 kb on + strandat 894.785 kb on - strandat 894.785 kb on - strandat 894.785 kb on - strandat 894.785 kb on - strandat 894.785 kb on - strandat 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.333 kb on + strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.334 kb on - strand, within Pf1N1B4_784at 895.351 kb on + strand, within Pf1N1B4_784at 895.351 kb on + strand, within Pf1N1B4_784at 895.351 kb on + strand, within Pf1N1B4_784at 895.352 kb on - strand, within Pf1N1B4_784at 895.352 kb on - strand, within Pf1N1B4_784at 895.352 kb on - strand, within Pf1N1B4_784at 895.402 kb on + strand, within Pf1N1B4_784at 895.402 kb on + strand, within Pf1N1B4_784at 895.402 kb on + strand, within Pf1N1B4_784at 895.403 kb on - strand, within Pf1N1B4_784at 895.403 kb on - strand, within Pf1N1B4_784at 895.403 kb on - strand, within Pf1N1B4_784at 895.606 kb on + strand, within Pf1N1B4_784at 895.606 kb on + strand, within Pf1N1B4_784at 895.606 kb on + strandat 895.606 kb on + strand, within Pf1N1B4_784at 895.606 kb on + strand, within Pf1N1B4_784at 895.606 kb on + strand, within Pf1N1B4_784at 895.606 kb on + strand, within Pf1N1B4_784at 895.607 kb on - strand, within Pf1N1B4_784at 895.607 kb on - strand, within Pf1N1B4_784at 895.607 kb on - strand, within Pf1N1B4_784at 895.607 kb on - strand, within Pf1N1B4_784at 895.607 kb on - strand, within Pf1N1B4_784at 895.607 kb on - strandat 895.607 kb on - strand, within Pf1N1B4_784at 895.607 kb on - strand, within Pf1N1B4_784at 895.607 kb on - strandat 895.621 kb on + strand, within Pf1N1B4_784at 895.621 kb on + strand, within Pf1N1B4_784at 895.622 kb on - strand, within Pf1N1B4_784at 895.670 kb on - strand, within Pf1N1B4_784at 895.705 kb on + strandat 895.706 kb on - strandat 895.854 kb on + strandat 895.854 kb on + strandat 895.854 kb on + strandat 895.855 kb on - strandat 895.863 kb on + strandat 895.863 kb on + strandat 895.863 kb on + strandat 895.863 kb on + strandat 895.863 kb on + strandat 895.863 kb on + strandat 895.863 kb on + strandat 895.863 kb on + strandat 895.863 kb on + strandat 895.864 kb on - strandat 895.961 kb on + strandat 895.961 kb on + strandat 895.962 kb on - strandat 896.114 kb on + strand, within Pf1N1B4_785at 896.115 kb on - strand, within Pf1N1B4_785at 896.178 kb on - strand, within Pf1N1B4_785at 896.178 kb on - strand, within Pf1N1B4_785at 896.316 kb on - strand, within Pf1N1B4_785at 896.316 kb on - strand, within Pf1N1B4_785at 896.316 kb on - strand, within Pf1N1B4_785at 896.416 kb on + strand, within Pf1N1B4_785at 896.417 kb on - strand, within Pf1N1B4_785at 896.417 kb on - strand, within Pf1N1B4_785at 896.417 kb on - strand, within Pf1N1B4_785at 896.646 kb on + strand, within Pf1N1B4_786at 896.647 kb on - strand, within Pf1N1B4_786at 896.647 kb on - strand, within Pf1N1B4_786

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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893,879 + Pf1N1B4_783 0.62 -0.1
893,999 + Pf1N1B4_783 0.67 -1.5
894,000 - Pf1N1B4_783 0.67 -0.1
894,029 + Pf1N1B4_783 0.69 -0.1
894,044 + Pf1N1B4_783 0.69 -1.4
894,045 - Pf1N1B4_783 0.69 -0.1
894,230 + Pf1N1B4_783 0.77 -3.1
894,230 + Pf1N1B4_783 0.77 -0.1
894,230 + Pf1N1B4_783 0.77 -1.6
894,231 - Pf1N1B4_783 0.77 -2.3
894,231 - Pf1N1B4_783 0.77 -0.1
894,231 - Pf1N1B4_783 0.77 -1.7
894,231 - Pf1N1B4_783 0.77 -2.5
894,357 - Pf1N1B4_783 0.83 -0.1
894,357 - Pf1N1B4_783 0.83 -1.4
894,362 + Pf1N1B4_783 0.83 -1.0
894,362 + Pf1N1B4_783 0.83 -0.1
894,362 + Pf1N1B4_783 0.83 -1.6
894,362 + Pf1N1B4_783 0.83 -1.7
894,363 - Pf1N1B4_783 0.83 -0.1
894,363 - -0.1
894,363 - Pf1N1B4_783 0.83 -0.1
894,366 + Pf1N1B4_783 0.83 -0.1
894,366 + Pf1N1B4_783 0.83 -0.1
894,366 + Pf1N1B4_783 0.83 -0.1
894,411 + Pf1N1B4_783 0.85 -0.1
894,411 + Pf1N1B4_783 0.85 -0.1
894,412 - Pf1N1B4_783 0.85 -0.1
894,412 - Pf1N1B4_783 0.85 -2.1
894,547 + -0.1
894,547 + -1.2
894,770 - -0.1
894,771 + -0.1
894,772 - -4.8
894,784 + -2.0
894,785 - -0.1
894,785 - -4.0
894,785 - -3.1
894,785 - -0.1
894,785 - -0.1
895,333 + Pf1N1B4_784 0.51 -0.1
895,333 + Pf1N1B4_784 0.51 -2.9
895,333 + Pf1N1B4_784 0.51 -0.1
895,333 + Pf1N1B4_784 0.51 -0.1
895,333 + Pf1N1B4_784 0.51 +0.9
895,333 + Pf1N1B4_784 0.51 -0.1
895,333 + Pf1N1B4_784 0.51 -3.2
895,333 + Pf1N1B4_784 0.51 -0.1
895,333 + Pf1N1B4_784 0.51 -0.1
895,333 + Pf1N1B4_784 0.51 -0.1
895,333 + Pf1N1B4_784 0.51 -0.1
895,333 + Pf1N1B4_784 0.51 -5.5
895,333 + Pf1N1B4_784 0.51 -0.1
895,334 - Pf1N1B4_784 0.51 -0.1
895,334 - Pf1N1B4_784 0.51 -0.1
895,334 - Pf1N1B4_784 0.51 -2.6
895,334 - Pf1N1B4_784 0.51 -5.3
895,334 - Pf1N1B4_784 0.51 -0.1
895,334 - Pf1N1B4_784 0.51 -0.1
895,334 - Pf1N1B4_784 0.51 -0.1
895,334 - Pf1N1B4_784 0.51 -0.1
895,334 - Pf1N1B4_784 0.51 -0.1
895,334 - Pf1N1B4_784 0.51 -4.5
895,334 - Pf1N1B4_784 0.51 -0.1
895,334 - Pf1N1B4_784 0.51 -2.6
895,334 - Pf1N1B4_784 0.51 -2.4
895,351 + Pf1N1B4_784 0.53 -0.1
895,351 + Pf1N1B4_784 0.53 -0.1
895,351 + Pf1N1B4_784 0.53 -2.6
895,352 - Pf1N1B4_784 0.53 -2.6
895,352 - Pf1N1B4_784 0.53 -0.1
895,352 - Pf1N1B4_784 0.53 -1.4
895,402 + Pf1N1B4_784 0.59 -4.0
895,402 + Pf1N1B4_784 0.59 -3.0
895,402 + Pf1N1B4_784 0.59 -0.1
895,403 - Pf1N1B4_784 0.59 -0.1
895,403 - Pf1N1B4_784 0.59 -0.1
895,403 - Pf1N1B4_784 0.59 -0.1
895,606 + Pf1N1B4_784 0.81 -0.1
895,606 + Pf1N1B4_784 0.81 -3.1
895,606 + -2.8
895,606 + Pf1N1B4_784 0.81 -0.1
895,606 + Pf1N1B4_784 0.81 -3.9
895,606 + Pf1N1B4_784 0.81 -0.1
895,606 + Pf1N1B4_784 0.81 -2.4
895,607 - Pf1N1B4_784 0.81 -2.9
895,607 - Pf1N1B4_784 0.81 -0.1
895,607 - Pf1N1B4_784 0.81 -0.1
895,607 - Pf1N1B4_784 0.81 -0.1
895,607 - Pf1N1B4_784 0.81 -3.4
895,607 - -0.1
895,607 - Pf1N1B4_784 0.81 -2.4
895,607 - Pf1N1B4_784 0.81 -0.1
895,607 - -0.1
895,621 + Pf1N1B4_784 0.83 -1.1
895,621 + Pf1N1B4_784 0.83 -0.1
895,622 - Pf1N1B4_784 0.83 -0.1
895,670 - Pf1N1B4_784 0.88 -0.1
895,705 + -0.1
895,706 - -2.9
895,854 + -0.1
895,854 + -0.1
895,854 + -0.1
895,855 - -0.1
895,863 + -0.1
895,863 + -2.7
895,863 + -0.1
895,863 + -1.4
895,863 + -1.8
895,863 + -0.1
895,863 + -0.1
895,863 + -2.8
895,863 + -0.1
895,864 - -3.7
895,961 + -0.1
895,961 + -0.1
895,962 - -0.1
896,114 + Pf1N1B4_785 0.10 -1.7
896,115 - Pf1N1B4_785 0.10 -0.1
896,178 - Pf1N1B4_785 0.22 -0.1
896,178 - Pf1N1B4_785 0.22 -0.1
896,316 - Pf1N1B4_785 0.49 -0.1
896,316 - Pf1N1B4_785 0.49 -0.1
896,316 - Pf1N1B4_785 0.49 -0.1
896,416 + Pf1N1B4_785 0.69 -1.9
896,417 - Pf1N1B4_785 0.69 -0.1
896,417 - Pf1N1B4_785 0.69 -3.1
896,417 - Pf1N1B4_785 0.69 -2.9
896,646 + Pf1N1B4_786 0.10 -3.1
896,647 - Pf1N1B4_786 0.11 -2.2
896,647 - Pf1N1B4_786 0.11 -0.1

Or see this region's nucleotide sequence