Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_74 and Pf1N1B4_75 are separated by 16 nucleotides Pf1N1B4_75 and Pf1N1B4_76 are separated by 501 nucleotides Pf1N1B4_76 and Pf1N1B4_77 are separated by 307 nucleotides Pf1N1B4_77 and Pf1N1B4_78 are separated by 143 nucleotides
Pf1N1B4_74 - hypothetical protein, at 78,166 to 78,429
_74
Pf1N1B4_75 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 78,446 to 78,823
_75
Pf1N1B4_76 - ATP binding protein, at 79,325 to 80,692
_76
Pf1N1B4_77 - FIG00956064: hypothetical protein, at 81,000 to 81,317
_77
Pf1N1B4_78 - FIG00956937: hypothetical protein, at 81,461 to 81,994
_78
Position (kb) 79
80
81 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 78.419 kb on + strand at 78.419 kb on + strand at 78.419 kb on + strand at 78.419 kb on + strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.685 kb on + strand, within Pf1N1B4_75 at 78.685 kb on + strand, within Pf1N1B4_75 at 78.686 kb on - strand, within Pf1N1B4_75 at 78.813 kb on - strand at 78.844 kb on + strand at 78.888 kb on + strand at 79.002 kb on + strand at 79.003 kb on - strand at 79.181 kb on + strand at 79.181 kb on + strand at 79.182 kb on - strand at 79.182 kb on - strand at 79.182 kb on - strand at 79.182 kb on - strand at 79.200 kb on + strand at 79.201 kb on - strand at 79.236 kb on - strand at 79.276 kb on + strand at 79.276 kb on + strand at 79.277 kb on - strand at 79.277 kb on - strand at 79.324 kb on - strand at 79.587 kb on + strand at 79.785 kb on - strand, within Pf1N1B4_76 at 80.281 kb on - strand, within Pf1N1B4_76 at 80.281 kb on - strand, within Pf1N1B4_76 at 80.344 kb on - strand, within Pf1N1B4_76 at 80.374 kb on - strand, within Pf1N1B4_76 at 80.449 kb on - strand, within Pf1N1B4_76 at 80.503 kb on - strand, within Pf1N1B4_76 at 80.659 kb on - strand at 80.705 kb on + strand at 80.752 kb on + strand at 80.752 kb on + strand at 80.752 kb on + strand at 80.752 kb on + strand at 80.753 kb on - strand at 80.753 kb on - strand at 80.753 kb on - strand at 80.753 kb on - strand at 80.824 kb on + strand at 80.824 kb on + strand at 80.824 kb on + strand at 80.825 kb on - strand at 80.825 kb on - strand at 80.877 kb on - strand at 80.877 kb on - strand at 80.911 kb on + strand at 80.927 kb on - strand at 81.030 kb on + strand at 81.030 kb on + strand at 81.031 kb on - strand at 81.071 kb on + strand, within Pf1N1B4_77 at 81.071 kb on + strand, within Pf1N1B4_77 at 81.071 kb on + strand, within Pf1N1B4_77 at 81.137 kb on + strand, within Pf1N1B4_77 at 81.162 kb on + strand, within Pf1N1B4_77 at 81.220 kb on + strand, within Pf1N1B4_77 at 81.289 kb on + strand at 81.290 kb on - strand at 81.290 kb on - strand at 81.315 kb on + strand at 81.316 kb on - strand at 81.334 kb on + strand at 81.335 kb on - strand at 81.335 kb on - strand at 81.403 kb on - strand at 81.403 kb on - strand at 81.426 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 78,419 + +0.0 78,419 + -3.1 78,419 + -3.7 78,419 + +0.0 78,420 - -1.1 78,420 - +0.0 78,420 - -2.6 78,420 - -2.5 78,420 - +0.0 78,685 + Pf1N1B4_75 0.63 +0.0 78,685 + Pf1N1B4_75 0.63 +0.0 78,686 - Pf1N1B4_75 0.63 +0.0 78,813 - -3.7 78,844 + -1.0 78,888 + +0.0 79,002 + +0.0 79,003 - +0.0 79,181 + +0.0 79,181 + +0.0 79,182 - -1.4 79,182 - -2.4 79,182 - +0.0 79,182 - +0.0 79,200 + +0.0 79,201 - +0.0 79,236 - +0.0 79,276 + -1.2 79,276 + -2.1 79,277 - -1.3 79,277 - -0.2 79,324 - +0.0 79,587 + -1.9 79,785 - Pf1N1B4_76 0.34 +0.0 80,281 - Pf1N1B4_76 0.70 +0.0 80,281 - Pf1N1B4_76 0.70 +0.0 80,344 - Pf1N1B4_76 0.74 +0.0 80,374 - Pf1N1B4_76 0.77 +0.0 80,449 - Pf1N1B4_76 0.82 -3.1 80,503 - Pf1N1B4_76 0.86 -4.6 80,659 - -1.5 80,705 + +0.0 80,752 + +0.0 80,752 + +0.0 80,752 + +0.0 80,752 + +0.0 80,753 - +0.0 80,753 - +0.0 80,753 - -1.6 80,753 - +0.0 80,824 + +0.0 80,824 + -1.4 80,824 + -2.3 80,825 - +0.0 80,825 - +0.0 80,877 - -2.9 80,877 - +0.0 80,911 + +0.0 80,927 - +0.0 81,030 + -3.7 81,030 + +0.0 81,031 - +0.0 81,071 + Pf1N1B4_77 0.22 +0.0 81,071 + Pf1N1B4_77 0.22 +0.0 81,071 + Pf1N1B4_77 0.22 +0.0 81,137 + Pf1N1B4_77 0.43 -3.0 81,162 + Pf1N1B4_77 0.51 +0.0 81,220 + Pf1N1B4_77 0.69 -3.4 81,289 + -3.8 81,290 - +0.0 81,290 - -2.8 81,315 + +0.0 81,316 - +0.0 81,334 + +0.0 81,335 - -3.5 81,335 - -2.1 81,403 - +0.0 81,403 - +0.0 81,426 + +0.0
Or see this region's nucleotide sequence