Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_700 and Pf1N1B4_701 are separated by 172 nucleotides Pf1N1B4_701 and Pf1N1B4_702 are separated by 9 nucleotides Pf1N1B4_702 and Pf1N1B4_703 are separated by 179 nucleotides
Pf1N1B4_700 - LysR-family transcriptional regulator clustered with PA0057, at 799,556 to 800,443
_700
Pf1N1B4_701 - FIG00954094: hypothetical protein, at 800,616 to 800,837
_701
Pf1N1B4_702 - Transcriptional regulator PobR, AraC family, at 800,847 to 801,758
_702
Pf1N1B4_703 - P-hydroxybenzoate hydroxylase (EC 1.14.13.2), at 801,938 to 803,131
_703
Position (kb)
800
801
802 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 800.048 kb on + strand, within Pf1N1B4_700 at 800.048 kb on + strand, within Pf1N1B4_700 at 800.049 kb on - strand, within Pf1N1B4_700 at 800.049 kb on - strand, within Pf1N1B4_700 at 800.117 kb on + strand, within Pf1N1B4_700 at 800.117 kb on + strand, within Pf1N1B4_700 at 800.118 kb on - strand, within Pf1N1B4_700 at 800.118 kb on - strand, within Pf1N1B4_700 at 800.336 kb on + strand, within Pf1N1B4_700 at 800.336 kb on + strand, within Pf1N1B4_700 at 800.336 kb on + strand, within Pf1N1B4_700 at 800.336 kb on + strand, within Pf1N1B4_700 at 800.336 kb on + strand, within Pf1N1B4_700 at 800.336 kb on + strand, within Pf1N1B4_700 at 800.336 kb on + strand, within Pf1N1B4_700 at 800.336 kb on + strand, within Pf1N1B4_700 at 800.337 kb on - strand, within Pf1N1B4_700 at 800.337 kb on - strand, within Pf1N1B4_700 at 800.337 kb on - strand, within Pf1N1B4_700 at 800.337 kb on - strand, within Pf1N1B4_700 at 800.337 kb on - strand, within Pf1N1B4_700 at 800.337 kb on - strand, within Pf1N1B4_700 at 800.346 kb on - strand, within Pf1N1B4_700 at 800.346 kb on - strand, within Pf1N1B4_700 at 800.346 kb on - strand, within Pf1N1B4_700 at 800.346 kb on - strand, within Pf1N1B4_700 at 800.404 kb on + strand at 800.405 kb on - strand at 800.405 kb on - strand at 800.405 kb on - strand at 800.405 kb on - strand at 800.441 kb on + strand at 800.441 kb on + strand at 800.441 kb on + strand at 800.441 kb on + strand at 800.441 kb on + strand at 800.442 kb on - strand at 800.442 kb on - strand at 800.442 kb on - strand at 800.442 kb on - strand at 800.442 kb on - strand at 800.442 kb on - strand at 800.442 kb on - strand at 800.442 kb on - strand at 800.442 kb on - strand at 800.565 kb on - strand at 800.615 kb on + strand at 800.615 kb on + strand at 800.615 kb on + strand at 800.616 kb on - strand at 800.616 kb on - strand at 800.616 kb on - strand at 800.616 kb on - strand at 800.616 kb on - strand at 800.616 kb on - strand at 800.616 kb on - strand at 800.640 kb on - strand, within Pf1N1B4_701 at 800.640 kb on - strand, within Pf1N1B4_701 at 800.682 kb on + strand, within Pf1N1B4_701 at 800.682 kb on + strand, within Pf1N1B4_701 at 800.682 kb on + strand, within Pf1N1B4_701 at 800.683 kb on - strand at 800.723 kb on - strand, within Pf1N1B4_701 at 800.723 kb on - strand, within Pf1N1B4_701 at 800.724 kb on + strand, within Pf1N1B4_701 at 800.724 kb on + strand, within Pf1N1B4_701 at 800.725 kb on - strand at 800.725 kb on - strand, within Pf1N1B4_701 at 800.989 kb on + strand, within Pf1N1B4_702 at 800.989 kb on + strand, within Pf1N1B4_702 at 800.990 kb on - strand, within Pf1N1B4_702 at 801.007 kb on + strand, within Pf1N1B4_702 at 801.008 kb on - strand, within Pf1N1B4_702 at 801.263 kb on + strand at 801.356 kb on - strand, within Pf1N1B4_702 at 801.597 kb on + strand, within Pf1N1B4_702 at 801.597 kb on + strand, within Pf1N1B4_702 at 801.597 kb on + strand, within Pf1N1B4_702 at 801.597 kb on + strand, within Pf1N1B4_702 at 801.597 kb on + strand, within Pf1N1B4_702 at 801.597 kb on + strand, within Pf1N1B4_702 at 801.598 kb on - strand, within Pf1N1B4_702 at 801.598 kb on - strand, within Pf1N1B4_702 at 801.681 kb on + strand at 801.681 kb on + strand at 801.681 kb on + strand at 801.715 kb on + strand at 801.715 kb on + strand at 801.715 kb on + strand at 801.715 kb on + strand at 801.716 kb on - strand at 801.816 kb on + strand at 801.816 kb on + strand at 801.816 kb on + strand at 801.817 kb on - strand at 801.817 kb on - strand at 801.930 kb on + strand at 801.993 kb on + strand at 802.058 kb on + strand, within Pf1N1B4_703 at 802.058 kb on + strand, within Pf1N1B4_703 at 802.058 kb on + strand, within Pf1N1B4_703 at 802.058 kb on + strand, within Pf1N1B4_703 at 802.058 kb on + strand, within Pf1N1B4_703 at 802.059 kb on - strand, within Pf1N1B4_703 at 802.059 kb on - strand, within Pf1N1B4_703 at 802.059 kb on - strand, within Pf1N1B4_703 at 802.059 kb on - strand, within Pf1N1B4_703 at 802.104 kb on + strand, within Pf1N1B4_703 at 802.104 kb on + strand, within Pf1N1B4_703 at 802.105 kb on - strand, within Pf1N1B4_703 at 802.105 kb on - strand, within Pf1N1B4_703 at 802.244 kb on + strand, within Pf1N1B4_703 at 802.245 kb on - strand, within Pf1N1B4_703 at 802.245 kb on - strand, within Pf1N1B4_703 at 802.310 kb on + strand, within Pf1N1B4_703 at 802.310 kb on + strand, within Pf1N1B4_703 at 802.310 kb on + strand, within Pf1N1B4_703 at 802.310 kb on + strand, within Pf1N1B4_703 at 802.310 kb on + strand, within Pf1N1B4_703 at 802.310 kb on + strand, within Pf1N1B4_703 at 802.310 kb on + strand, within Pf1N1B4_703 at 802.310 kb on + strand, within Pf1N1B4_703 at 802.311 kb on - strand, within Pf1N1B4_703 at 802.311 kb on - strand, within Pf1N1B4_703 at 802.311 kb on - strand, within Pf1N1B4_703 at 802.311 kb on - strand, within Pf1N1B4_703 at 802.388 kb on + strand, within Pf1N1B4_703 at 802.388 kb on + strand, within Pf1N1B4_703 at 802.406 kb on + strand, within Pf1N1B4_703 at 802.406 kb on + strand, within Pf1N1B4_703 at 802.407 kb on - strand, within Pf1N1B4_703 at 802.407 kb on - strand, within Pf1N1B4_703 at 802.601 kb on - strand, within Pf1N1B4_703 at 802.610 kb on + strand, within Pf1N1B4_703 at 802.611 kb on - strand, within Pf1N1B4_703 at 802.611 kb on - strand, within Pf1N1B4_703 at 802.611 kb on - strand, within Pf1N1B4_703
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 800,048 + Pf1N1B4_700 0.55 -0.0 800,048 + Pf1N1B4_700 0.55 -1.8 800,049 - Pf1N1B4_700 0.56 -2.1 800,049 - Pf1N1B4_700 0.56 -1.6 800,117 + Pf1N1B4_700 0.63 -0.0 800,117 + Pf1N1B4_700 0.63 -2.7 800,118 - Pf1N1B4_700 0.63 -0.0 800,118 - Pf1N1B4_700 0.63 -0.0 800,336 + Pf1N1B4_700 0.88 -0.0 800,336 + Pf1N1B4_700 0.88 -0.0 800,336 + Pf1N1B4_700 0.88 -2.4 800,336 + Pf1N1B4_700 0.88 -0.0 800,336 + Pf1N1B4_700 0.88 -0.0 800,336 + Pf1N1B4_700 0.88 -1.6 800,336 + Pf1N1B4_700 0.88 -0.0 800,336 + Pf1N1B4_700 0.88 -0.0 800,337 - Pf1N1B4_700 0.88 -2.3 800,337 - Pf1N1B4_700 0.88 -1.0 800,337 - Pf1N1B4_700 0.88 -0.0 800,337 - Pf1N1B4_700 0.88 -0.0 800,337 - Pf1N1B4_700 0.88 -0.0 800,337 - Pf1N1B4_700 0.88 -0.0 800,346 - Pf1N1B4_700 0.89 -0.0 800,346 - Pf1N1B4_700 0.89 -0.0 800,346 - Pf1N1B4_700 0.89 -0.0 800,346 - Pf1N1B4_700 0.89 -0.0 800,404 + -0.0 800,405 - -0.2 800,405 - -2.6 800,405 - -2.0 800,405 - -0.8 800,441 + -0.0 800,441 + -0.0 800,441 + -0.0 800,441 + -0.0 800,441 + -0.0 800,442 - -0.0 800,442 - -1.8 800,442 - -1.6 800,442 - -0.0 800,442 - -0.0 800,442 - -1.8 800,442 - -0.0 800,442 - -0.0 800,442 - -0.0 800,565 - -0.0 800,615 + -1.0 800,615 + -0.0 800,615 + -0.0 800,616 - -1.9 800,616 - -2.7 800,616 - -2.5 800,616 - -1.4 800,616 - -0.0 800,616 - -2.8 800,616 - -0.0 800,640 - Pf1N1B4_701 0.11 -0.0 800,640 - Pf1N1B4_701 0.11 -0.0 800,682 + Pf1N1B4_701 0.30 -0.0 800,682 + Pf1N1B4_701 0.30 -2.1 800,682 + Pf1N1B4_701 0.30 -2.2 800,683 - -1.0 800,723 - Pf1N1B4_701 0.48 -0.0 800,723 - Pf1N1B4_701 0.48 -0.0 800,724 + Pf1N1B4_701 0.49 -2.2 800,724 + Pf1N1B4_701 0.49 -0.0 800,725 - -2.1 800,725 - Pf1N1B4_701 0.49 +0.2 800,989 + Pf1N1B4_702 0.16 -2.8 800,989 + Pf1N1B4_702 0.16 -3.7 800,990 - Pf1N1B4_702 0.16 -4.1 801,007 + Pf1N1B4_702 0.18 -0.0 801,008 - Pf1N1B4_702 0.18 -3.3 801,263 + -0.0 801,356 - Pf1N1B4_702 0.56 -2.1 801,597 + Pf1N1B4_702 0.82 -0.0 801,597 + Pf1N1B4_702 0.82 -0.0 801,597 + Pf1N1B4_702 0.82 -0.0 801,597 + Pf1N1B4_702 0.82 -0.8 801,597 + Pf1N1B4_702 0.82 -4.2 801,597 + Pf1N1B4_702 0.82 -0.0 801,598 - Pf1N1B4_702 0.82 -0.0 801,598 - Pf1N1B4_702 0.82 -0.0 801,681 + -0.0 801,681 + -0.0 801,681 + -0.0 801,715 + -0.0 801,715 + -0.0 801,715 + -0.0 801,715 + -0.0 801,716 - -0.0 801,816 + -0.0 801,816 + -0.0 801,816 + -0.0 801,817 - -0.0 801,817 - -3.5 801,930 + -0.0 801,993 + -4.1 802,058 + Pf1N1B4_703 0.10 -0.0 802,058 + Pf1N1B4_703 0.10 -0.0 802,058 + Pf1N1B4_703 0.10 -2.4 802,058 + Pf1N1B4_703 0.10 +0.8 802,058 + Pf1N1B4_703 0.10 -0.0 802,059 - Pf1N1B4_703 0.10 -1.5 802,059 - Pf1N1B4_703 0.10 -2.2 802,059 - Pf1N1B4_703 0.10 -0.0 802,059 - Pf1N1B4_703 0.10 -0.0 802,104 + Pf1N1B4_703 0.14 -0.0 802,104 + Pf1N1B4_703 0.14 -0.0 802,105 - Pf1N1B4_703 0.14 -0.0 802,105 - Pf1N1B4_703 0.14 -1.0 802,244 + Pf1N1B4_703 0.26 -0.9 802,245 - Pf1N1B4_703 0.26 -0.0 802,245 - Pf1N1B4_703 0.26 -0.0 802,310 + Pf1N1B4_703 0.31 -0.0 802,310 + Pf1N1B4_703 0.31 -3.2 802,310 + Pf1N1B4_703 0.31 -0.0 802,310 + Pf1N1B4_703 0.31 -0.0 802,310 + Pf1N1B4_703 0.31 -0.0 802,310 + Pf1N1B4_703 0.31 -5.5 802,310 + Pf1N1B4_703 0.31 -0.0 802,310 + Pf1N1B4_703 0.31 -0.0 802,311 - Pf1N1B4_703 0.31 -0.0 802,311 - Pf1N1B4_703 0.31 -0.0 802,311 - Pf1N1B4_703 0.31 -0.0 802,311 - Pf1N1B4_703 0.31 -0.0 802,388 + Pf1N1B4_703 0.38 -0.0 802,388 + Pf1N1B4_703 0.38 -1.2 802,406 + Pf1N1B4_703 0.39 -1.8 802,406 + Pf1N1B4_703 0.39 -0.0 802,407 - Pf1N1B4_703 0.39 -2.4 802,407 - Pf1N1B4_703 0.39 -0.0 802,601 - Pf1N1B4_703 0.56 -1.5 802,610 + Pf1N1B4_703 0.56 -2.6 802,611 - Pf1N1B4_703 0.56 -0.0 802,611 - Pf1N1B4_703 0.56 -1.7 802,611 - Pf1N1B4_703 0.56 -0.0
Or see this region's nucleotide sequence