Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_626

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_624 and Pf1N1B4_625 are separated by 95 nucleotidesPf1N1B4_625 and Pf1N1B4_626 are separated by 135 nucleotidesPf1N1B4_626 and Pf1N1B4_627 overlap by 33 nucleotidesPf1N1B4_627 and Pf1N1B4_5174 are separated by 170 nucleotidesPf1N1B4_5174 and Pf1N1B4_628 are separated by 63 nucleotides Pf1N1B4_624 - Cold shock protein CspA, at 723,499 to 723,711 _624 Pf1N1B4_625 - FIG023103: Predicted transmembrane protein, at 723,807 to 724,295 _625 Pf1N1B4_626 - Quinolinate synthetase (EC 2.5.1.72), at 724,431 to 725,489 _626 Pf1N1B4_627 - hypothetical protein, at 725,457 to 725,573 _627 Pf1N1B4_5174 - tRNA-Lys-TTT, at 725,744 to 725,816 _5174 Pf1N1B4_628 - Queuosine Biosynthesis QueC ATPase, at 725,880 to 726,560 _628 Position (kb) 724 725 726Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 723.445 kb on - strandat 723.695 kb on + strandat 723.695 kb on + strandat 723.695 kb on + strandat 723.737 kb on + strandat 723.766 kb on + strandat 723.766 kb on + strandat 723.767 kb on - strandat 723.775 kb on - strandat 723.823 kb on + strandat 723.823 kb on + strandat 723.823 kb on + strandat 723.823 kb on + strandat 723.823 kb on + strandat 723.823 kb on + strandat 723.823 kb on + strandat 723.823 kb on + strandat 723.823 kb on + strandat 723.823 kb on + strandat 723.823 kb on + strandat 723.823 kb on + strandat 723.824 kb on - strandat 723.824 kb on - strandat 723.824 kb on - strandat 723.824 kb on - strandat 723.824 kb on - strandat 723.824 kb on - strandat 723.824 kb on - strandat 723.824 kb on - strandat 723.824 kb on - strandat 723.824 kb on - strandat 723.882 kb on + strand, within Pf1N1B4_625at 723.883 kb on - strand, within Pf1N1B4_625at 723.883 kb on - strand, within Pf1N1B4_625at 723.883 kb on - strand, within Pf1N1B4_625at 723.896 kb on - strand, within Pf1N1B4_625at 723.896 kb on - strand, within Pf1N1B4_625at 723.930 kb on + strand, within Pf1N1B4_625at 723.930 kb on + strand, within Pf1N1B4_625at 723.931 kb on - strand, within Pf1N1B4_625at 723.931 kb on - strand, within Pf1N1B4_625at 723.931 kb on - strand, within Pf1N1B4_625at 724.016 kb on - strand, within Pf1N1B4_625at 724.186 kb on + strand, within Pf1N1B4_625at 724.187 kb on - strand, within Pf1N1B4_625at 724.251 kb on + strandat 724.251 kb on + strandat 724.251 kb on + strandat 724.251 kb on + strandat 724.251 kb on + strandat 724.251 kb on + strandat 724.251 kb on + strandat 724.251 kb on + strandat 724.252 kb on - strandat 724.252 kb on - strandat 724.252 kb on - strandat 724.252 kb on - strandat 724.252 kb on - strandat 724.252 kb on - strandat 724.270 kb on + strandat 724.270 kb on + strandat 724.270 kb on + strandat 724.347 kb on + strandat 724.348 kb on - strandat 724.348 kb on - strandat 724.348 kb on - strandat 724.610 kb on + strand, within Pf1N1B4_626at 724.935 kb on - strand, within Pf1N1B4_626at 724.936 kb on + strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 725.072 kb on - strandat 725.072 kb on - strand, within Pf1N1B4_626at 725.072 kb on - strand, within Pf1N1B4_626at 725.084 kb on + strand, within Pf1N1B4_626at 725.084 kb on + strand, within Pf1N1B4_626at 725.085 kb on - strand, within Pf1N1B4_626at 725.085 kb on - strand, within Pf1N1B4_626at 725.341 kb on + strand, within Pf1N1B4_626at 725.341 kb on + strand, within Pf1N1B4_626at 725.342 kb on - strand, within Pf1N1B4_626at 725.397 kb on - strandat 725.404 kb on + strandat 725.405 kb on - strandat 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strandat 725.576 kb on - strandat 725.576 kb on - strandat 725.576 kb on - strandat 725.715 kb on - strandat 725.715 kb on - strandat 725.715 kb on - strandat 725.715 kb on - strandat 725.887 kb on + strandat 725.887 kb on + strandat 725.955 kb on - strand, within Pf1N1B4_628at 726.096 kb on + strand, within Pf1N1B4_628at 726.097 kb on - strand, within Pf1N1B4_628at 726.097 kb on - strand, within Pf1N1B4_628at 726.176 kb on - strand, within Pf1N1B4_628at 726.176 kb on - strand, within Pf1N1B4_628at 726.274 kb on + strand, within Pf1N1B4_628at 726.274 kb on + strand, within Pf1N1B4_628at 726.274 kb on + strand, within Pf1N1B4_628at 726.274 kb on + strand, within Pf1N1B4_628at 726.275 kb on - strand, within Pf1N1B4_628at 726.275 kb on - strand, within Pf1N1B4_628at 726.275 kb on - strand, within Pf1N1B4_628at 726.320 kb on + strand, within Pf1N1B4_628at 726.320 kb on + strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.446 kb on - strand, within Pf1N1B4_628at 726.463 kb on + strand, within Pf1N1B4_628at 726.463 kb on + strand, within Pf1N1B4_628at 726.463 kb on + strand, within Pf1N1B4_628at 726.464 kb on - strand, within Pf1N1B4_628at 726.464 kb on - strand, within Pf1N1B4_628

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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723,445 - -0.9
723,695 + -0.0
723,695 + -0.0
723,695 + -0.5
723,737 + -0.0
723,766 + -1.8
723,766 + -0.0
723,767 - -0.0
723,775 - -1.9
723,823 + -1.2
723,823 + -1.3
723,823 + -1.1
723,823 + -0.0
723,823 + -2.7
723,823 + -0.0
723,823 + -0.0
723,823 + -1.0
723,823 + -2.5
723,823 + -0.0
723,823 + -1.3
723,823 + -1.0
723,824 - -0.0
723,824 - -0.0
723,824 - -1.1
723,824 - -2.0
723,824 - -0.0
723,824 - -0.0
723,824 - -0.0
723,824 - -0.0
723,824 - -2.6
723,824 - -0.6
723,882 + Pf1N1B4_625 0.15 -0.9
723,883 - Pf1N1B4_625 0.16 +1.8
723,883 - Pf1N1B4_625 0.16 -0.0
723,883 - Pf1N1B4_625 0.16 -0.0
723,896 - Pf1N1B4_625 0.18 -0.0
723,896 - Pf1N1B4_625 0.18 -0.0
723,930 + Pf1N1B4_625 0.25 -0.0
723,930 + Pf1N1B4_625 0.25 -0.0
723,931 - Pf1N1B4_625 0.25 -0.0
723,931 - Pf1N1B4_625 0.25 -0.0
723,931 - Pf1N1B4_625 0.25 -1.0
724,016 - Pf1N1B4_625 0.43 -1.0
724,186 + Pf1N1B4_625 0.78 -1.7
724,187 - Pf1N1B4_625 0.78 -0.0
724,251 + -0.7
724,251 + -0.0
724,251 + -0.0
724,251 + -2.0
724,251 + -0.0
724,251 + -0.0
724,251 + -0.0
724,251 + -1.8
724,252 - -2.9
724,252 - -0.7
724,252 - -0.0
724,252 - -1.0
724,252 - -0.0
724,252 - -0.0
724,270 + -0.8
724,270 + -0.0
724,270 + +0.3
724,347 + -2.8
724,348 - -0.0
724,348 - -0.0
724,348 - -0.7
724,610 + Pf1N1B4_626 0.17 -2.7
724,935 - Pf1N1B4_626 0.48 -0.0
724,936 + Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -1.9
724,937 - Pf1N1B4_626 0.48 -1.9
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -1.9
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -1.5
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -3.1
725,072 - -0.0
725,072 - Pf1N1B4_626 0.61 -3.0
725,072 - Pf1N1B4_626 0.61 -0.0
725,084 + Pf1N1B4_626 0.62 -0.0
725,084 + Pf1N1B4_626 0.62 -0.0
725,085 - Pf1N1B4_626 0.62 -0.0
725,085 - Pf1N1B4_626 0.62 -3.0
725,341 + Pf1N1B4_626 0.86 -0.0
725,341 + Pf1N1B4_626 0.86 -0.0
725,342 - Pf1N1B4_626 0.86 -0.0
725,397 - -0.0
725,404 + -0.0
725,405 - -1.4
725,532 - Pf1N1B4_627 0.64 -0.0
725,532 - Pf1N1B4_627 0.64 -1.4
725,532 - Pf1N1B4_627 0.64 -2.0
725,532 - Pf1N1B4_627 0.64 -0.0
725,532 - Pf1N1B4_627 0.64 -2.2
725,532 - Pf1N1B4_627 0.64 -0.0
725,532 - Pf1N1B4_627 0.64 -0.0
725,532 - -0.0
725,576 - -0.2
725,576 - -0.0
725,576 - -0.0
725,715 - -0.0
725,715 - -0.0
725,715 - -0.0
725,715 - -0.0
725,887 + -0.0
725,887 + -0.0
725,955 - Pf1N1B4_628 0.11 -1.4
726,096 + Pf1N1B4_628 0.32 -2.5
726,097 - Pf1N1B4_628 0.32 -0.0
726,097 - Pf1N1B4_628 0.32 -0.0
726,176 - Pf1N1B4_628 0.43 -0.0
726,176 - Pf1N1B4_628 0.43 -3.0
726,274 + Pf1N1B4_628 0.58 -0.0
726,274 + Pf1N1B4_628 0.58 -0.0
726,274 + Pf1N1B4_628 0.58 -0.0
726,274 + Pf1N1B4_628 0.58 -0.0
726,275 - Pf1N1B4_628 0.58 -0.0
726,275 - Pf1N1B4_628 0.58 -2.1
726,275 - Pf1N1B4_628 0.58 -0.8
726,320 + Pf1N1B4_628 0.65 -0.0
726,320 + Pf1N1B4_628 0.65 -0.0
726,321 - Pf1N1B4_628 0.65 -0.0
726,321 - Pf1N1B4_628 0.65 -1.2
726,321 - Pf1N1B4_628 0.65 -0.0
726,321 - Pf1N1B4_628 0.65 -2.7
726,321 - Pf1N1B4_628 0.65 +1.0
726,321 - Pf1N1B4_628 0.65 -2.6
726,446 - Pf1N1B4_628 0.83 -0.0
726,463 + Pf1N1B4_628 0.86 +1.0
726,463 + Pf1N1B4_628 0.86 -0.0
726,463 + Pf1N1B4_628 0.86 -0.0
726,464 - Pf1N1B4_628 0.86 -0.0
726,464 - Pf1N1B4_628 0.86 -0.0

Or see this region's nucleotide sequence