Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5955 and Pf1N1B4_5956 are separated by 9 nucleotides Pf1N1B4_5956 and Pf1N1B4_5957 overlap by 1 nucleotides Pf1N1B4_5957 and Pf1N1B4_5958 are separated by 183 nucleotides
Pf1N1B4_5955 - Permease of the major facilitator superfamily, at 403,412 to 404,641
_5955
Pf1N1B4_5956 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 404,651 to 405,703
_5956
Pf1N1B4_5957 - Arsenical resistance operon repressor, at 405,703 to 406,065
_5957
Pf1N1B4_5958 - Ferrichrome-iron receptor, at 406,249 to 408,372
_5958
Position (kb) 404
405
406 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 403.920 kb on + strand, within Pf1N1B4_5955 at 403.920 kb on + strand, within Pf1N1B4_5955 at 404.220 kb on + strand, within Pf1N1B4_5955 at 404.220 kb on + strand, within Pf1N1B4_5955 at 404.221 kb on - strand, within Pf1N1B4_5955 at 404.475 kb on + strand at 404.476 kb on - strand, within Pf1N1B4_5955 at 404.476 kb on - strand, within Pf1N1B4_5955 at 404.476 kb on - strand at 404.476 kb on - strand, within Pf1N1B4_5955 at 404.581 kb on - strand at 404.581 kb on - strand at 404.602 kb on - strand at 404.727 kb on + strand at 404.727 kb on + strand at 404.727 kb on + strand at 404.728 kb on - strand at 404.728 kb on - strand at 404.728 kb on - strand at 404.728 kb on - strand at 405.334 kb on - strand, within Pf1N1B4_5956 at 405.650 kb on - strand at 405.852 kb on - strand, within Pf1N1B4_5957 at 405.854 kb on + strand, within Pf1N1B4_5957 at 405.855 kb on - strand, within Pf1N1B4_5957 at 405.897 kb on + strand, within Pf1N1B4_5957 at 405.898 kb on - strand, within Pf1N1B4_5957 at 406.106 kb on + strand at 406.133 kb on - strand at 406.149 kb on - strand at 406.270 kb on + strand at 406.307 kb on + strand at 406.308 kb on - strand at 406.308 kb on - strand at 406.388 kb on - strand at 406.603 kb on + strand, within Pf1N1B4_5958 at 406.603 kb on + strand, within Pf1N1B4_5958 at 406.604 kb on - strand, within Pf1N1B4_5958 at 406.604 kb on - strand, within Pf1N1B4_5958 at 406.604 kb on - strand, within Pf1N1B4_5958 at 406.604 kb on - strand, within Pf1N1B4_5958 at 406.604 kb on - strand, within Pf1N1B4_5958 at 406.604 kb on - strand at 406.634 kb on - strand, within Pf1N1B4_5958 at 406.634 kb on - strand, within Pf1N1B4_5958
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 403,920 + Pf1N1B4_5955 0.41 +0.0 403,920 + Pf1N1B4_5955 0.41 +0.0 404,220 + Pf1N1B4_5955 0.66 -3.0 404,220 + Pf1N1B4_5955 0.66 +0.0 404,221 - Pf1N1B4_5955 0.66 -2.9 404,475 + +0.0 404,476 - Pf1N1B4_5955 0.87 +0.0 404,476 - Pf1N1B4_5955 0.87 -1.3 404,476 - +0.0 404,476 - Pf1N1B4_5955 0.87 -1.6 404,581 - -2.3 404,581 - +0.0 404,602 - +0.0 404,727 + +0.0 404,727 + -2.0 404,727 + +0.0 404,728 - -1.9 404,728 - -3.2 404,728 - +0.0 404,728 - +0.0 405,334 - Pf1N1B4_5956 0.65 +0.0 405,650 - +0.0 405,852 - Pf1N1B4_5957 0.41 +0.0 405,854 + Pf1N1B4_5957 0.42 +0.0 405,855 - Pf1N1B4_5957 0.42 -1.5 405,897 + Pf1N1B4_5957 0.53 -2.7 405,898 - Pf1N1B4_5957 0.54 -2.1 406,106 + -1.4 406,133 - -1.5 406,149 - +0.0 406,270 + +0.0 406,307 + -0.6 406,308 - -2.7 406,308 - +0.0 406,388 - +0.0 406,603 + Pf1N1B4_5958 0.17 -2.2 406,603 + Pf1N1B4_5958 0.17 +0.0 406,604 - Pf1N1B4_5958 0.17 -3.5 406,604 - Pf1N1B4_5958 0.17 +0.0 406,604 - Pf1N1B4_5958 0.17 +0.0 406,604 - Pf1N1B4_5958 0.17 +0.0 406,604 - Pf1N1B4_5958 0.17 +0.0 406,604 - +0.0 406,634 - Pf1N1B4_5958 0.18 +0.0 406,634 - Pf1N1B4_5958 0.18 -1.7
Or see this region's nucleotide sequence