Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5907 and Pf1N1B4_5908 are separated by 89 nucleotides Pf1N1B4_5908 and Pf1N1B4_5909 are separated by 70 nucleotides
Pf1N1B4_5907 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 349,909 to 351,336
_5907
Pf1N1B4_5908 - Nicotinamidase family protein YcaC, at 351,426 to 352,052
_5908
Pf1N1B4_5909 - Exoenzymes regulatory protein AepA precursor, at 352,123 to 353,961
_5909
Position (kb) 351
352
353 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 350.728 kb on + strand, within Pf1N1B4_5907 at 350.956 kb on + strand, within Pf1N1B4_5907 at 350.956 kb on + strand at 351.166 kb on + strand, within Pf1N1B4_5907 at 351.167 kb on - strand, within Pf1N1B4_5907 at 351.367 kb on + strand at 351.367 kb on + strand at 351.367 kb on + strand at 351.391 kb on + strand at 351.391 kb on + strand at 351.469 kb on + strand at 351.709 kb on - strand, within Pf1N1B4_5908 at 352.005 kb on + strand at 352.037 kb on - strand at 352.037 kb on - strand at 352.079 kb on - strand at 352.079 kb on - strand at 352.124 kb on + strand at 352.124 kb on + strand at 352.124 kb on + strand at 352.617 kb on - strand, within Pf1N1B4_5909 at 352.617 kb on - strand, within Pf1N1B4_5909 at 352.638 kb on - strand, within Pf1N1B4_5909 at 352.638 kb on - strand, within Pf1N1B4_5909 at 352.638 kb on - strand, within Pf1N1B4_5909 at 352.638 kb on - strand, within Pf1N1B4_5909 at 352.638 kb on - strand, within Pf1N1B4_5909 at 352.752 kb on - strand, within Pf1N1B4_5909 at 352.752 kb on - strand at 352.931 kb on + strand, within Pf1N1B4_5909 at 352.931 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 350,728 + Pf1N1B4_5907 0.57 +0.0 350,956 + Pf1N1B4_5907 0.73 -0.7 350,956 + +0.0 351,166 + Pf1N1B4_5907 0.88 +0.0 351,167 - Pf1N1B4_5907 0.88 +0.0 351,367 + -4.4 351,367 + +0.0 351,367 + -2.6 351,391 + +0.0 351,391 + -1.3 351,469 + -2.3 351,709 - Pf1N1B4_5908 0.45 +0.0 352,005 + +0.0 352,037 - -2.0 352,037 - +0.0 352,079 - +0.0 352,079 - +0.0 352,124 + -1.8 352,124 + -2.9 352,124 + +0.0 352,617 - Pf1N1B4_5909 0.27 -1.8 352,617 - Pf1N1B4_5909 0.27 -2.0 352,638 - Pf1N1B4_5909 0.28 -1.7 352,638 - Pf1N1B4_5909 0.28 +0.0 352,638 - Pf1N1B4_5909 0.28 +0.0 352,638 - Pf1N1B4_5909 0.28 +0.0 352,638 - Pf1N1B4_5909 0.28 -0.5 352,752 - Pf1N1B4_5909 0.34 +0.0 352,752 - +0.0 352,931 + Pf1N1B4_5909 0.44 +0.0 352,931 + +0.0
Or see this region's nucleotide sequence