Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5652 and Pf1N1B4_5653 are separated by 149 nucleotides Pf1N1B4_5653 and Pf1N1B4_5654 are separated by 3 nucleotides
Pf1N1B4_5652 - ChlI component of cobalt chelatase involved in B12 biosynthesis, at 80,438 to 81,448
_5652
Pf1N1B4_5653 - CobN component of cobalt chelatase involved in B12 biosynthesis, at 81,598 to 85,464
_5653
Pf1N1B4_5654 - CobW GTPase involved in cobalt insertion for B12 biosynthesis, at 85,468 to 86,529
_5654
Position (kb) 81
82
83
84
85
86 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 81.141 kb on + strand, within Pf1N1B4_5652 at 81.141 kb on + strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.171 kb on - strand, within Pf1N1B4_5652 at 81.614 kb on - strand at 81.614 kb on - strand at 81.768 kb on - strand at 81.768 kb on - strand at 81.870 kb on + strand at 81.870 kb on + strand at 81.871 kb on - strand at 81.871 kb on - strand at 82.058 kb on + strand, within Pf1N1B4_5653 at 82.778 kb on + strand at 82.951 kb on + strand, within Pf1N1B4_5653 at 82.952 kb on - strand, within Pf1N1B4_5653 at 83.311 kb on - strand, within Pf1N1B4_5653 at 83.414 kb on + strand, within Pf1N1B4_5653 at 83.414 kb on + strand, within Pf1N1B4_5653 at 83.415 kb on - strand, within Pf1N1B4_5653 at 83.415 kb on - strand, within Pf1N1B4_5653 at 83.570 kb on + strand at 83.571 kb on - strand, within Pf1N1B4_5653 at 83.612 kb on + strand, within Pf1N1B4_5653 at 83.717 kb on + strand, within Pf1N1B4_5653 at 83.717 kb on + strand, within Pf1N1B4_5653 at 83.840 kb on + strand at 83.841 kb on - strand, within Pf1N1B4_5653 at 83.862 kb on - strand at 83.862 kb on - strand, within Pf1N1B4_5653 at 83.874 kb on - strand at 84.089 kb on + strand, within Pf1N1B4_5653 at 84.972 kb on - strand, within Pf1N1B4_5653 at 85.253 kb on + strand at 85.254 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 81,141 + Pf1N1B4_5652 0.70 -0.0 81,141 + Pf1N1B4_5652 0.70 +0.0 81,142 - Pf1N1B4_5652 0.70 +0.0 81,142 - Pf1N1B4_5652 0.70 +0.0 81,142 - Pf1N1B4_5652 0.70 -1.4 81,142 - Pf1N1B4_5652 0.70 +0.0 81,142 - Pf1N1B4_5652 0.70 -0.1 81,171 - Pf1N1B4_5652 0.73 +0.0 81,614 - +0.0 81,614 - -3.1 81,768 - +0.0 81,768 - -1.2 81,870 + -3.7 81,870 + -1.1 81,871 - +0.0 81,871 - -1.5 82,058 + Pf1N1B4_5653 0.12 -2.7 82,778 + -3.4 82,951 + Pf1N1B4_5653 0.35 +0.0 82,952 - Pf1N1B4_5653 0.35 -3.4 83,311 - Pf1N1B4_5653 0.44 -1.0 83,414 + Pf1N1B4_5653 0.47 -1.0 83,414 + Pf1N1B4_5653 0.47 -1.4 83,415 - Pf1N1B4_5653 0.47 +0.0 83,415 - Pf1N1B4_5653 0.47 +0.0 83,570 + -1.1 83,571 - Pf1N1B4_5653 0.51 +0.0 83,612 + Pf1N1B4_5653 0.52 -3.0 83,717 + Pf1N1B4_5653 0.55 -3.0 83,717 + Pf1N1B4_5653 0.55 +0.0 83,840 + +0.0 83,841 - Pf1N1B4_5653 0.58 -1.8 83,862 - -1.8 83,862 - Pf1N1B4_5653 0.59 -2.8 83,874 - -1.9 84,089 + Pf1N1B4_5653 0.64 -3.1 84,972 - Pf1N1B4_5653 0.87 -2.0 85,253 + -2.7 85,254 - +0.0
Or see this region's nucleotide sequence