Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5608 and Pf1N1B4_5609 overlap by 13 nucleotides Pf1N1B4_5609 and Pf1N1B4_5610 are separated by 65 nucleotides Pf1N1B4_5610 and Pf1N1B4_5611 are separated by 19 nucleotides
Pf1N1B4_5608 - Sulfopyruvate decarboxylase - alpha subunit (EC 4.1.1.79), at 34,681 to 35,220
_5608
Pf1N1B4_5609 - Sulfopyruvate decarboxylase - beta subunit (EC 4.1.1.79), at 35,208 to 35,804
_5609
Pf1N1B4_5610 - Probable acyl-CoA dehydrogenase (EC 1.3.99.3), at 35,870 to 37,033
_5610
Pf1N1B4_5611 - L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16), at 37,053 to 38,255
_5611
Position (kb) 35
36
37
38 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 35.316 kb on + strand, within Pf1N1B4_5609 at 35.355 kb on + strand, within Pf1N1B4_5609 at 35.355 kb on + strand, within Pf1N1B4_5609 at 35.355 kb on + strand, within Pf1N1B4_5609 at 35.355 kb on + strand, within Pf1N1B4_5609 at 35.757 kb on + strand at 35.811 kb on + strand at 36.112 kb on + strand, within Pf1N1B4_5610 at 36.141 kb on - strand, within Pf1N1B4_5610 at 36.189 kb on - strand at 36.192 kb on - strand, within Pf1N1B4_5610 at 36.192 kb on - strand, within Pf1N1B4_5610 at 36.308 kb on + strand, within Pf1N1B4_5610 at 36.308 kb on + strand, within Pf1N1B4_5610 at 36.313 kb on - strand, within Pf1N1B4_5610 at 36.330 kb on + strand, within Pf1N1B4_5610 at 36.330 kb on + strand at 36.579 kb on - strand, within Pf1N1B4_5610 at 36.839 kb on + strand, within Pf1N1B4_5610 at 36.840 kb on - strand, within Pf1N1B4_5610 at 36.992 kb on + strand at 36.993 kb on - strand at 36.993 kb on - strand at 36.993 kb on - strand at 37.031 kb on + strand at 37.032 kb on - strand at 37.032 kb on - strand at 37.590 kb on + strand at 37.591 kb on - strand, within Pf1N1B4_5611 at 37.705 kb on - strand, within Pf1N1B4_5611 at 37.708 kb on - strand, within Pf1N1B4_5611
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 35,316 + Pf1N1B4_5609 0.18 -2.6 35,355 + Pf1N1B4_5609 0.25 -2.0 35,355 + Pf1N1B4_5609 0.25 +0.0 35,355 + Pf1N1B4_5609 0.25 +0.0 35,355 + Pf1N1B4_5609 0.25 -1.6 35,757 + +0.0 35,811 + +0.0 36,112 + Pf1N1B4_5610 0.21 +0.0 36,141 - Pf1N1B4_5610 0.23 -5.3 36,189 - +0.0 36,192 - Pf1N1B4_5610 0.28 +0.0 36,192 - Pf1N1B4_5610 0.28 -3.5 36,308 + Pf1N1B4_5610 0.38 +0.0 36,308 + Pf1N1B4_5610 0.38 -3.7 36,313 - Pf1N1B4_5610 0.38 -3.0 36,330 + Pf1N1B4_5610 0.40 +0.0 36,330 + -4.2 36,579 - Pf1N1B4_5610 0.61 -4.5 36,839 + Pf1N1B4_5610 0.83 -3.3 36,840 - Pf1N1B4_5610 0.83 +0.0 36,992 + +0.0 36,993 - -5.2 36,993 - -4.1 36,993 - +0.0 37,031 + -3.3 37,032 - +0.0 37,032 - -2.5 37,590 + +0.0 37,591 - Pf1N1B4_5611 0.45 +0.0 37,705 - Pf1N1B4_5611 0.54 -3.9 37,708 - Pf1N1B4_5611 0.54 +0.0
Or see this region's nucleotide sequence