Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5551 and Pf1N1B4_5552 are separated by 182 nucleotides Pf1N1B4_5552 and Pf1N1B4_5553 are separated by 394 nucleotides
Pf1N1B4_5551 - hypothetical protein, at 323,086 to 324,072
_5551
Pf1N1B4_5552 - hypothetical protein, at 324,255 to 324,395
_5552
Pf1N1B4_5553 - Exodeoxyribonuclease I (EC 3.1.11.1), at 324,790 to 326,202
_5553
Position (kb) 324
325 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 323.464 kb on - strand, within Pf1N1B4_5551 at 323.464 kb on - strand, within Pf1N1B4_5551 at 323.498 kb on + strand, within Pf1N1B4_5551 at 323.499 kb on - strand, within Pf1N1B4_5551 at 323.564 kb on - strand, within Pf1N1B4_5551 at 323.576 kb on + strand, within Pf1N1B4_5551 at 323.576 kb on + strand, within Pf1N1B4_5551 at 323.576 kb on + strand at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.792 kb on - strand, within Pf1N1B4_5551 at 323.935 kb on + strand, within Pf1N1B4_5551 at 324.012 kb on + strand at 324.059 kb on + strand at 324.092 kb on + strand at 324.210 kb on - strand at 324.210 kb on - strand at 324.251 kb on + strand at 324.252 kb on - strand at 324.262 kb on + strand at 324.262 kb on + strand at 324.263 kb on - strand at 324.306 kb on + strand, within Pf1N1B4_5552 at 324.307 kb on - strand, within Pf1N1B4_5552 at 324.307 kb on - strand, within Pf1N1B4_5552 at 324.307 kb on - strand, within Pf1N1B4_5552 at 324.335 kb on + strand, within Pf1N1B4_5552 at 324.360 kb on - strand, within Pf1N1B4_5552 at 324.360 kb on - strand, within Pf1N1B4_5552 at 324.582 kb on + strand at 324.582 kb on + strand at 324.583 kb on - strand at 324.604 kb on - strand at 324.699 kb on + strand at 324.700 kb on - strand at 324.721 kb on + strand at 324.722 kb on - strand at 324.722 kb on - strand at 324.882 kb on + strand at 324.882 kb on + strand at 324.883 kb on - strand at 324.883 kb on - strand at 324.883 kb on - strand at 324.883 kb on - strand at 324.926 kb on - strand at 325.004 kb on + strand at 325.005 kb on - strand, within Pf1N1B4_5553 at 325.130 kb on + strand, within Pf1N1B4_5553 at 325.130 kb on + strand, within Pf1N1B4_5553 at 325.135 kb on - strand at 325.240 kb on + strand, within Pf1N1B4_5553 at 325.240 kb on + strand at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 323,464 - Pf1N1B4_5551 0.38 +0.1 323,464 - Pf1N1B4_5551 0.38 +0.1 323,498 + Pf1N1B4_5551 0.42 +0.1 323,499 - Pf1N1B4_5551 0.42 +0.1 323,564 - Pf1N1B4_5551 0.48 -1.5 323,576 + Pf1N1B4_5551 0.50 +0.1 323,576 + Pf1N1B4_5551 0.50 -2.0 323,576 + -0.9 323,577 - Pf1N1B4_5551 0.50 +0.1 323,577 - Pf1N1B4_5551 0.50 +0.1 323,577 - Pf1N1B4_5551 0.50 -3.1 323,791 + Pf1N1B4_5551 0.71 -1.6 323,791 + Pf1N1B4_5551 0.71 +0.1 323,791 + Pf1N1B4_5551 0.71 -2.5 323,792 - Pf1N1B4_5551 0.72 -2.9 323,935 + Pf1N1B4_5551 0.86 +0.1 324,012 + +0.1 324,059 + -1.8 324,092 + -1.1 324,210 - +0.1 324,210 - -0.9 324,251 + +0.1 324,252 - +0.1 324,262 + -1.2 324,262 + -1.9 324,263 - +0.1 324,306 + Pf1N1B4_5552 0.36 +0.1 324,307 - Pf1N1B4_5552 0.37 +0.1 324,307 - Pf1N1B4_5552 0.37 -3.6 324,307 - Pf1N1B4_5552 0.37 +0.1 324,335 + Pf1N1B4_5552 0.57 +0.1 324,360 - Pf1N1B4_5552 0.74 +0.1 324,360 - Pf1N1B4_5552 0.74 +0.1 324,582 + +0.1 324,582 + +0.1 324,583 - -3.1 324,604 - -1.9 324,699 + -1.6 324,700 - -1.6 324,721 + -3.2 324,722 - +0.1 324,722 - +0.1 324,882 + +0.1 324,882 + +0.1 324,883 - -1.7 324,883 - -1.6 324,883 - +0.1 324,883 - +0.1 324,926 - +0.1 325,004 + -1.8 325,005 - Pf1N1B4_5553 0.15 +0.1 325,130 + Pf1N1B4_5553 0.24 +0.1 325,130 + Pf1N1B4_5553 0.24 +0.1 325,135 - -1.2 325,240 + Pf1N1B4_5553 0.32 +0.1 325,240 + -1.9 325,241 - Pf1N1B4_5553 0.32 +0.1 325,241 - Pf1N1B4_5553 0.32 -2.2 325,241 - Pf1N1B4_5553 0.32 +0.1 325,241 - Pf1N1B4_5553 0.32 -2.1 325,241 - Pf1N1B4_5553 0.32 -2.6
Or see this region's nucleotide sequence