Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5506

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5504 and Pf1N1B4_5505 are separated by 38 nucleotidesPf1N1B4_5505 and Pf1N1B4_5506 overlap by 10 nucleotidesPf1N1B4_5506 and Pf1N1B4_5507 are separated by 48 nucleotides Pf1N1B4_5504 - hypothetical protein, at 254,478 to 255,089 _5504 Pf1N1B4_5505 - hypothetical protein, at 255,128 to 255,304 _5505 Pf1N1B4_5506 - hypothetical protein, at 255,295 to 255,819 _5506 Pf1N1B4_5507 - hypothetical protein, at 255,868 to 256,542 _5507 Position (kb) 255 256Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 254.417 kb on + strandat 254.418 kb on - strandat 254.501 kb on - strandat 254.538 kb on + strandat 254.539 kb on - strandat 254.539 kb on - strandat 254.581 kb on + strand, within Pf1N1B4_5504at 254.581 kb on + strand, within Pf1N1B4_5504at 254.582 kb on - strand, within Pf1N1B4_5504at 254.582 kb on - strand, within Pf1N1B4_5504at 254.582 kb on - strand, within Pf1N1B4_5504at 254.702 kb on - strand, within Pf1N1B4_5504at 254.730 kb on - strand, within Pf1N1B4_5504at 254.730 kb on - strand, within Pf1N1B4_5504at 254.769 kb on + strand, within Pf1N1B4_5504at 254.769 kb on - strand, within Pf1N1B4_5504at 254.770 kb on - strand, within Pf1N1B4_5504at 254.770 kb on - strand, within Pf1N1B4_5504at 254.770 kb on - strand, within Pf1N1B4_5504at 254.770 kb on - strand, within Pf1N1B4_5504at 254.770 kb on - strand, within Pf1N1B4_5504at 254.770 kb on - strand, within Pf1N1B4_5504at 254.770 kb on - strand, within Pf1N1B4_5504at 254.770 kb on - strand, within Pf1N1B4_5504at 254.834 kb on - strand, within Pf1N1B4_5504at 254.836 kb on - strand, within Pf1N1B4_5504at 254.836 kb on - strand, within Pf1N1B4_5504at 254.836 kb on - strand, within Pf1N1B4_5504at 254.893 kb on + strand, within Pf1N1B4_5504at 254.894 kb on - strand, within Pf1N1B4_5504at 254.894 kb on - strand, within Pf1N1B4_5504at 255.054 kb on + strandat 255.055 kb on - strandat 255.066 kb on + strandat 255.067 kb on - strandat 255.067 kb on - strandat 255.131 kb on + strandat 255.131 kb on + strandat 255.131 kb on + strandat 255.131 kb on + strandat 255.131 kb on + strandat 255.131 kb on + strandat 255.131 kb on + strandat 255.132 kb on - strandat 255.132 kb on - strandat 255.132 kb on - strandat 255.132 kb on - strandat 255.132 kb on - strandat 255.132 kb on - strandat 255.132 kb on - strandat 255.242 kb on + strand, within Pf1N1B4_5505at 255.242 kb on + strand, within Pf1N1B4_5505at 255.242 kb on + strandat 255.242 kb on + strand, within Pf1N1B4_5505at 255.242 kb on + strand, within Pf1N1B4_5505at 255.242 kb on + strandat 255.242 kb on + strand, within Pf1N1B4_5505at 255.242 kb on + strand, within Pf1N1B4_5505at 255.242 kb on + strand, within Pf1N1B4_5505at 255.243 kb on - strand, within Pf1N1B4_5505at 255.243 kb on - strandat 255.269 kb on + strandat 255.269 kb on + strandat 255.269 kb on + strand, within Pf1N1B4_5505at 255.269 kb on + strand, within Pf1N1B4_5505at 255.270 kb on - strand, within Pf1N1B4_5505at 255.270 kb on - strand, within Pf1N1B4_5505at 255.289 kb on + strandat 255.592 kb on + strand, within Pf1N1B4_5506at 255.592 kb on + strand, within Pf1N1B4_5506at 255.592 kb on + strand, within Pf1N1B4_5506at 255.592 kb on + strand, within Pf1N1B4_5506at 255.592 kb on + strandat 255.592 kb on + strand, within Pf1N1B4_5506at 255.593 kb on - strand, within Pf1N1B4_5506at 255.593 kb on - strand, within Pf1N1B4_5506at 255.593 kb on - strandat 255.593 kb on - strandat 255.689 kb on + strand, within Pf1N1B4_5506at 255.689 kb on + strand, within Pf1N1B4_5506at 255.689 kb on + strand, within Pf1N1B4_5506at 255.689 kb on + strand, within Pf1N1B4_5506at 255.691 kb on + strandat 255.692 kb on - strand, within Pf1N1B4_5506at 255.740 kb on + strand, within Pf1N1B4_5506at 255.741 kb on - strand, within Pf1N1B4_5506at 255.741 kb on - strand, within Pf1N1B4_5506at 255.829 kb on + strandat 255.829 kb on + strandat 255.829 kb on + strandat 255.830 kb on - strandat 255.881 kb on + strandat 255.881 kb on + strandat 255.881 kb on + strandat 255.882 kb on - strandat 255.932 kb on + strandat 255.933 kb on - strandat 255.933 kb on - strandat 255.933 kb on - strandat 255.933 kb on - strandat 255.933 kb on - strandat 255.933 kb on - strandat 256.353 kb on - strand, within Pf1N1B4_5507at 256.405 kb on + strand, within Pf1N1B4_5507at 256.405 kb on + strand, within Pf1N1B4_5507at 256.406 kb on - strand, within Pf1N1B4_5507at 256.490 kb on + strandat 256.491 kb on - strandat 256.491 kb on - strandat 256.662 kb on + strandat 256.662 kb on + strandat 256.680 kb on - strandat 256.720 kb on + strandat 256.720 kb on + strandat 256.721 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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254,417 + +0.1
254,418 - +0.1
254,501 - -1.1
254,538 + +0.1
254,539 - -0.9
254,539 - -1.6
254,581 + Pf1N1B4_5504 0.17 -0.9
254,581 + Pf1N1B4_5504 0.17 -1.9
254,582 - Pf1N1B4_5504 0.17 -1.7
254,582 - Pf1N1B4_5504 0.17 -2.4
254,582 - Pf1N1B4_5504 0.17 -2.5
254,702 - Pf1N1B4_5504 0.37 -1.8
254,730 - Pf1N1B4_5504 0.41 -2.2
254,730 - Pf1N1B4_5504 0.41 +0.1
254,769 + Pf1N1B4_5504 0.48 +0.1
254,769 - Pf1N1B4_5504 0.48 -1.1
254,770 - Pf1N1B4_5504 0.48 +0.1
254,770 - Pf1N1B4_5504 0.48 +0.1
254,770 - Pf1N1B4_5504 0.48 -3.1
254,770 - Pf1N1B4_5504 0.48 -2.1
254,770 - Pf1N1B4_5504 0.48 -1.8
254,770 - Pf1N1B4_5504 0.48 +0.1
254,770 - Pf1N1B4_5504 0.48 -1.5
254,770 - Pf1N1B4_5504 0.48 +0.1
254,834 - Pf1N1B4_5504 0.58 -1.7
254,836 - Pf1N1B4_5504 0.58 -2.1
254,836 - Pf1N1B4_5504 0.58 -1.7
254,836 - Pf1N1B4_5504 0.58 -2.0
254,893 + Pf1N1B4_5504 0.68 +0.1
254,894 - Pf1N1B4_5504 0.68 -3.0
254,894 - Pf1N1B4_5504 0.68 +0.1
255,054 + +0.1
255,055 - -2.9
255,066 + -2.3
255,067 - -1.4
255,067 - +0.1
255,131 + +0.1
255,131 + -3.4
255,131 + +0.1
255,131 + -1.5
255,131 + -2.5
255,131 + +0.1
255,131 + +0.1
255,132 - -2.3
255,132 - +0.1
255,132 - +0.1
255,132 - -2.3
255,132 - -1.6
255,132 - +0.1
255,132 - +0.1
255,242 + Pf1N1B4_5505 0.64 +0.1
255,242 + Pf1N1B4_5505 0.64 +0.1
255,242 + +0.1
255,242 + Pf1N1B4_5505 0.64 -2.9
255,242 + Pf1N1B4_5505 0.64 +0.1
255,242 + -2.0
255,242 + Pf1N1B4_5505 0.64 -3.5
255,242 + Pf1N1B4_5505 0.64 +0.1
255,242 + Pf1N1B4_5505 0.64 -3.2
255,243 - Pf1N1B4_5505 0.65 -2.9
255,243 - -2.8
255,269 + -2.1
255,269 + +0.1
255,269 + Pf1N1B4_5505 0.80 +0.1
255,269 + Pf1N1B4_5505 0.80 +0.1
255,270 - Pf1N1B4_5505 0.80 -2.6
255,270 - Pf1N1B4_5505 0.80 +0.1
255,289 + +0.1
255,592 + Pf1N1B4_5506 0.57 -3.1
255,592 + Pf1N1B4_5506 0.57 +0.1
255,592 + Pf1N1B4_5506 0.57 +0.1
255,592 + Pf1N1B4_5506 0.57 +0.1
255,592 + +0.1
255,592 + Pf1N1B4_5506 0.57 -1.4
255,593 - Pf1N1B4_5506 0.57 -3.6
255,593 - Pf1N1B4_5506 0.57 -3.4
255,593 - -2.1
255,593 - +0.1
255,689 + Pf1N1B4_5506 0.75 -4.9
255,689 + Pf1N1B4_5506 0.75 -2.4
255,689 + Pf1N1B4_5506 0.75 +0.1
255,689 + Pf1N1B4_5506 0.75 +0.1
255,691 + -0.7
255,692 - Pf1N1B4_5506 0.76 -2.9
255,740 + Pf1N1B4_5506 0.85 +0.1
255,741 - Pf1N1B4_5506 0.85 -3.2
255,741 - Pf1N1B4_5506 0.85 +0.1
255,829 + +0.1
255,829 + +0.1
255,829 + -2.1
255,830 - -1.6
255,881 + +0.1
255,881 + -3.4
255,881 + +0.1
255,882 - +0.1
255,932 + +0.1
255,933 - +0.1
255,933 - +0.1
255,933 - +0.1
255,933 - -3.2
255,933 - -3.3
255,933 - +0.1
256,353 - Pf1N1B4_5507 0.72 -1.9
256,405 + Pf1N1B4_5507 0.80 +0.1
256,405 + Pf1N1B4_5507 0.80 +0.1
256,406 - Pf1N1B4_5507 0.80 +0.1
256,490 + +0.1
256,491 - -1.3
256,491 - +0.1
256,662 + +0.1
256,662 + +0.1
256,680 - -1.5
256,720 + +0.1
256,720 + +0.1
256,721 - -2.1

Or see this region's nucleotide sequence