Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5271 and Pf1N1B4_5272 are separated by 255 nucleotides Pf1N1B4_5272 and Pf1N1B4_5273 are separated by 123 nucleotides Pf1N1B4_5273 and Pf1N1B4_5274 are separated by 176 nucleotides Pf1N1B4_5274 and Pf1N1B4_5275 are separated by 424 nucleotides
Pf1N1B4_5271 - hypothetical protein, at 28,584 to 28,769
_5271
Pf1N1B4_5272 - hypothetical protein, at 29,025 to 29,201
_5272
Pf1N1B4_5273 - hypothetical protein, at 29,325 to 29,489
_5273
Pf1N1B4_5274 - hypothetical protein, at 29,666 to 29,947
_5274
Pf1N1B4_5275 - hypothetical protein, at 30,372 to 30,725
_5275
Position (kb) 29
30 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 28.732 kb on - strand, within Pf1N1B4_5271 at 28.836 kb on + strand at 28.837 kb on - strand at 28.945 kb on - strand at 28.948 kb on - strand at 28.948 kb on - strand at 28.971 kb on - strand at 28.971 kb on - strand at 28.971 kb on - strand at 28.989 kb on + strand at 29.212 kb on + strand at 29.594 kb on + strand at 29.594 kb on + strand at 29.595 kb on - strand at 29.595 kb on - strand at 29.595 kb on - strand at 29.595 kb on - strand at 29.595 kb on - strand at 29.656 kb on + strand at 29.656 kb on + strand at 29.708 kb on + strand, within Pf1N1B4_5274 at 29.709 kb on - strand, within Pf1N1B4_5274 at 29.709 kb on - strand, within Pf1N1B4_5274 at 29.780 kb on + strand, within Pf1N1B4_5274 at 29.780 kb on + strand, within Pf1N1B4_5274 at 29.780 kb on + strand, within Pf1N1B4_5274 at 29.780 kb on + strand, within Pf1N1B4_5274 at 29.781 kb on - strand, within Pf1N1B4_5274 at 29.781 kb on - strand, within Pf1N1B4_5274 at 29.872 kb on + strand, within Pf1N1B4_5274 at 29.872 kb on + strand, within Pf1N1B4_5274 at 29.912 kb on + strand, within Pf1N1B4_5274 at 29.912 kb on + strand, within Pf1N1B4_5274 at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.925 kb on - strand at 29.925 kb on - strand at 29.925 kb on - strand at 29.945 kb on + strand at 29.945 kb on + strand at 29.946 kb on - strand at 29.946 kb on - strand at 29.985 kb on + strand at 29.985 kb on + strand at 29.985 kb on + strand at 30.143 kb on + strand at 30.143 kb on + strand at 30.143 kb on + strand at 30.143 kb on + strand at 30.144 kb on - strand at 30.144 kb on - strand at 30.144 kb on - strand at 30.144 kb on - strand at 30.144 kb on - strand at 30.144 kb on - strand at 30.212 kb on - strand at 30.212 kb on - strand at 30.278 kb on + strand at 30.297 kb on + strand at 30.383 kb on + strand at 30.384 kb on - strand at 30.384 kb on - strand at 30.564 kb on + strand, within Pf1N1B4_5275 at 30.565 kb on - strand, within Pf1N1B4_5275 at 30.565 kb on - strand, within Pf1N1B4_5275 at 30.600 kb on + strand, within Pf1N1B4_5275 at 30.600 kb on + strand at 30.600 kb on + strand, within Pf1N1B4_5275 at 30.723 kb on + strand at 30.724 kb on - strand at 30.780 kb on + strand at 30.805 kb on + strand at 30.904 kb on + strand at 30.919 kb on + strand at 30.919 kb on + strand at 30.945 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 28,732 - Pf1N1B4_5271 0.80 -0.8 28,836 + -1.6 28,837 - +0.1 28,945 - +0.1 28,948 - +0.1 28,948 - +0.1 28,971 - -3.6 28,971 - -4.9 28,971 - +0.1 28,989 + -1.6 29,212 + +0.1 29,594 + +0.1 29,594 + +0.1 29,595 - +0.1 29,595 - +0.1 29,595 - +0.1 29,595 - +0.1 29,595 - +0.1 29,656 + -1.3 29,656 + +0.1 29,708 + Pf1N1B4_5274 0.15 -3.2 29,709 - Pf1N1B4_5274 0.15 +0.1 29,709 - Pf1N1B4_5274 0.15 +0.1 29,780 + Pf1N1B4_5274 0.40 -3.5 29,780 + Pf1N1B4_5274 0.40 +0.1 29,780 + Pf1N1B4_5274 0.40 +0.1 29,780 + Pf1N1B4_5274 0.40 -3.2 29,781 - Pf1N1B4_5274 0.41 +0.1 29,781 - Pf1N1B4_5274 0.41 +0.1 29,872 + Pf1N1B4_5274 0.73 +0.1 29,872 + Pf1N1B4_5274 0.73 +0.1 29,912 + Pf1N1B4_5274 0.87 -2.7 29,912 + Pf1N1B4_5274 0.87 +0.1 29,924 + -1.0 29,924 + +0.1 29,924 + -3.0 29,924 + +0.1 29,924 + +0.1 29,924 + +0.1 29,924 + -1.7 29,924 + +0.1 29,924 + +0.1 29,925 - -2.7 29,925 - +0.1 29,925 - -0.8 29,945 + -2.7 29,945 + +0.1 29,946 - -1.6 29,946 - -0.8 29,985 + +0.1 29,985 + -2.6 29,985 + +0.1 30,143 + -0.4 30,143 + +0.1 30,143 + +0.1 30,143 + -2.3 30,144 - +0.1 30,144 - +0.1 30,144 - -1.1 30,144 - -2.1 30,144 - -1.9 30,144 - -1.7 30,212 - +0.1 30,212 - +0.1 30,278 + +0.1 30,297 + +0.1 30,383 + +0.1 30,384 - +0.1 30,384 - +0.1 30,564 + Pf1N1B4_5275 0.54 -0.8 30,565 - Pf1N1B4_5275 0.55 +0.1 30,565 - Pf1N1B4_5275 0.55 +0.1 30,600 + Pf1N1B4_5275 0.64 -1.6 30,600 + -3.7 30,600 + Pf1N1B4_5275 0.64 -1.8 30,723 + +0.1 30,724 - +0.1 30,780 + -2.0 30,805 + -1.6 30,904 + +0.1 30,919 + -2.9 30,919 + +0.1 30,945 + +1.1
Or see this region's nucleotide sequence