Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5255 and Pf1N1B4_5256 are separated by 149 nucleotides Pf1N1B4_5256 and Pf1N1B4_5257 are separated by 546 nucleotides
Pf1N1B4_5255 - hypothetical protein, at 14,928 to 15,152
_5255
Pf1N1B4_5256 - hypothetical protein, at 15,302 to 15,460
_5256
Pf1N1B4_5257 - hypothetical protein, at 16,007 to 16,306
_5257
Position (kb) 14
15
16 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 13.964 kb on - strand at 14.101 kb on + strand at 14.148 kb on + strand at 14.148 kb on + strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.162 kb on + strand at 14.162 kb on + strand at 14.163 kb on - strand at 14.163 kb on - strand at 14.246 kb on + strand at 14.364 kb on - strand at 14.364 kb on - strand at 14.364 kb on - strand at 14.364 kb on - strand at 14.401 kb on + strand at 14.401 kb on + strand at 14.401 kb on + strand at 14.402 kb on - strand at 14.539 kb on + strand at 14.539 kb on + strand at 14.604 kb on + strand at 14.605 kb on - strand at 14.612 kb on - strand at 14.668 kb on + strand at 14.669 kb on - strand at 14.669 kb on - strand at 14.669 kb on - strand at 14.669 kb on - strand at 14.669 kb on - strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.675 kb on - strand at 14.692 kb on + strand at 14.692 kb on + strand at 14.693 kb on - strand at 14.693 kb on - strand at 14.701 kb on - strand at 14.701 kb on - strand at 14.701 kb on - strand at 14.701 kb on - strand at 14.706 kb on + strand at 14.706 kb on + strand at 14.707 kb on - strand at 14.707 kb on - strand at 14.895 kb on - strand at 14.917 kb on - strand at 14.985 kb on - strand, within Pf1N1B4_5255 at 15.028 kb on + strand, within Pf1N1B4_5255 at 15.073 kb on + strand, within Pf1N1B4_5255 at 15.074 kb on - strand, within Pf1N1B4_5255 at 15.125 kb on + strand, within Pf1N1B4_5255 at 15.126 kb on - strand, within Pf1N1B4_5255 at 15.126 kb on - strand, within Pf1N1B4_5255 at 15.126 kb on - strand, within Pf1N1B4_5255 at 15.249 kb on - strand at 15.298 kb on - strand at 15.303 kb on + strand at 15.332 kb on + strand, within Pf1N1B4_5256 at 15.333 kb on - strand, within Pf1N1B4_5256 at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.499 kb on - strand at 15.499 kb on - strand at 15.581 kb on - strand at 15.604 kb on + strand at 15.757 kb on + strand at 15.758 kb on - strand at 15.758 kb on - strand at 15.794 kb on + strand at 15.794 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.037 kb on + strand at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 13,964 - +0.1 14,101 + -1.2 14,148 + -3.6 14,148 + -2.1 14,149 - +0.1 14,149 - +0.1 14,149 - -2.5 14,149 - -3.9 14,149 - -2.2 14,162 + -2.0 14,162 + -2.8 14,163 - +0.1 14,163 - -2.6 14,246 + +0.1 14,364 - -2.3 14,364 - +0.1 14,364 - +0.1 14,364 - +0.1 14,401 + +0.1 14,401 + +1.1 14,401 + +0.1 14,402 - +0.1 14,539 + -3.2 14,539 + -2.9 14,604 + +0.1 14,605 - +0.1 14,612 - +0.1 14,668 + -3.2 14,669 - +0.1 14,669 - -2.8 14,669 - +0.1 14,669 - -3.2 14,669 - +0.1 14,670 + +0.1 14,670 + +0.1 14,670 + -3.1 14,670 + -3.7 14,670 + +0.1 14,670 + +0.1 14,670 + +0.1 14,670 + +0.1 14,670 + +0.1 14,670 + +0.1 14,671 - -1.8 14,671 - +0.1 14,671 - +0.1 14,671 - +0.1 14,671 - +0.1 14,671 - +0.1 14,671 - +0.1 14,671 - +0.1 14,671 - -1.6 14,671 - +0.1 14,671 - -3.1 14,671 - -2.1 14,671 - -2.6 14,671 - +0.1 14,671 - -1.4 14,671 - +0.1 14,671 - -0.6 14,671 - +0.1 14,671 - -1.4 14,671 - +0.1 14,671 - +0.1 14,671 - +0.1 14,675 - -2.9 14,692 + +0.1 14,692 + -1.8 14,693 - +0.1 14,693 - +0.1 14,701 - -2.0 14,701 - +0.1 14,701 - +0.1 14,701 - +0.1 14,706 + +0.1 14,706 + -2.8 14,707 - +0.1 14,707 - +0.1 14,895 - +0.1 14,917 - +0.1 14,985 - Pf1N1B4_5255 0.25 +0.1 15,028 + Pf1N1B4_5255 0.44 -3.6 15,073 + Pf1N1B4_5255 0.64 -3.9 15,074 - Pf1N1B4_5255 0.65 +0.1 15,125 + Pf1N1B4_5255 0.88 -0.9 15,126 - Pf1N1B4_5255 0.88 +0.1 15,126 - Pf1N1B4_5255 0.88 +0.1 15,126 - Pf1N1B4_5255 0.88 +0.1 15,249 - +0.1 15,298 - -0.9 15,303 + +0.1 15,332 + Pf1N1B4_5256 0.19 -1.9 15,333 - Pf1N1B4_5256 0.19 +0.1 15,498 + +0.1 15,498 + -1.6 15,498 + +0.1 15,498 + -3.1 15,498 + -2.9 15,498 + +0.1 15,499 - +0.1 15,499 - -2.1 15,581 - +0.1 15,604 + +0.1 15,757 + +0.1 15,758 - +0.1 15,758 - -1.9 15,794 + -1.9 15,794 + -1.9 15,889 + -2.4 15,889 + -1.8 15,889 + +0.1 15,889 + +0.1 15,889 + +0.1 15,889 + +0.1 15,889 + +0.1 15,889 + +0.1 15,889 + +0.1 15,889 + +0.1 15,890 - +0.1 15,890 - +0.1 15,890 - -1.5 15,890 - -0.6 15,890 - -1.0 15,890 - +0.1 15,890 - +0.1 15,890 - +0.1 15,890 - +0.1 16,037 + Pf1N1B4_5257 0.10 -2.2 16,037 + Pf1N1B4_5257 0.10 -4.2 16,037 + -2.7 16,037 + Pf1N1B4_5257 0.10 +0.1 16,038 - Pf1N1B4_5257 0.10 +0.1 16,038 - Pf1N1B4_5257 0.10 +0.1 16,038 - Pf1N1B4_5257 0.10 -1.6 16,038 - Pf1N1B4_5257 0.10 -0.6
Or see this region's nucleotide sequence