Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5137 and Pf1N1B4_5138 are separated by 237 nucleotides Pf1N1B4_5138 and Pf1N1B4_5139 are separated by 113 nucleotides
Pf1N1B4_5137 - Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-), at 5,716,073 to 5,717,170
_5137
Pf1N1B4_5138 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,717,408 to 5,718,499
_5138
Pf1N1B4_5139 - Gamma-aminobutyrate permease, at 5,718,613 to 5,720,118
_5139
Position (kb) 5717
5718
5719 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5716.419 kb on + strand, within Pf1N1B4_5137 at 5716.851 kb on + strand at 5716.942 kb on - strand, within Pf1N1B4_5137 at 5717.005 kb on + strand, within Pf1N1B4_5137 at 5717.006 kb on - strand, within Pf1N1B4_5137 at 5717.006 kb on - strand, within Pf1N1B4_5137 at 5717.006 kb on - strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.020 kb on - strand at 5717.020 kb on - strand at 5717.020 kb on - strand, within Pf1N1B4_5137 at 5717.020 kb on - strand, within Pf1N1B4_5137 at 5717.020 kb on - strand, within Pf1N1B4_5137 at 5717.151 kb on + strand at 5717.151 kb on + strand at 5717.151 kb on + strand at 5717.152 kb on - strand at 5717.152 kb on - strand at 5717.154 kb on + strand at 5717.155 kb on - strand at 5717.338 kb on + strand at 5717.339 kb on - strand at 5717.633 kb on + strand, within Pf1N1B4_5138 at 5717.694 kb on + strand, within Pf1N1B4_5138 at 5717.721 kb on + strand, within Pf1N1B4_5138 at 5717.721 kb on + strand, within Pf1N1B4_5138 at 5717.767 kb on - strand at 5717.911 kb on - strand, within Pf1N1B4_5138 at 5718.492 kb on + strand at 5718.492 kb on + strand at 5718.492 kb on + strand at 5718.598 kb on - strand at 5718.607 kb on + strand at 5718.607 kb on + strand at 5718.608 kb on - strand at 5718.790 kb on - strand, within Pf1N1B4_5139 at 5718.790 kb on - strand, within Pf1N1B4_5139 at 5718.950 kb on + strand, within Pf1N1B4_5139 at 5719.089 kb on + strand, within Pf1N1B4_5139 at 5719.091 kb on + strand, within Pf1N1B4_5139 at 5719.091 kb on + strand at 5719.092 kb on - strand, within Pf1N1B4_5139 at 5719.141 kb on + strand, within Pf1N1B4_5139 at 5719.148 kb on - strand, within Pf1N1B4_5139
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 5,716,419 + Pf1N1B4_5137 0.32 -2.6 5,716,851 + +0.0 5,716,942 - Pf1N1B4_5137 0.79 +0.0 5,717,005 + Pf1N1B4_5137 0.85 -1.5 5,717,006 - Pf1N1B4_5137 0.85 +0.0 5,717,006 - Pf1N1B4_5137 0.85 -4.2 5,717,006 - Pf1N1B4_5137 0.85 -2.0 5,717,019 + Pf1N1B4_5137 0.86 +0.0 5,717,019 + Pf1N1B4_5137 0.86 -2.9 5,717,019 + Pf1N1B4_5137 0.86 +0.0 5,717,019 + Pf1N1B4_5137 0.86 -2.8 5,717,019 + Pf1N1B4_5137 0.86 +0.0 5,717,019 + Pf1N1B4_5137 0.86 -4.9 5,717,019 + Pf1N1B4_5137 0.86 -1.8 5,717,020 - -1.8 5,717,020 - -2.3 5,717,020 - Pf1N1B4_5137 0.86 -2.4 5,717,020 - Pf1N1B4_5137 0.86 -3.3 5,717,020 - Pf1N1B4_5137 0.86 +0.0 5,717,151 + +0.0 5,717,151 + +0.0 5,717,151 + +0.0 5,717,152 - +0.0 5,717,152 - -1.9 5,717,154 + +0.0 5,717,155 - +0.0 5,717,338 + -0.0 5,717,339 - +0.0 5,717,633 + Pf1N1B4_5138 0.21 +0.0 5,717,694 + Pf1N1B4_5138 0.26 -2.9 5,717,721 + Pf1N1B4_5138 0.29 -2.5 5,717,721 + Pf1N1B4_5138 0.29 -2.3 5,717,767 - +0.0 5,717,911 - Pf1N1B4_5138 0.46 +0.0 5,718,492 + +0.0 5,718,492 + -1.8 5,718,492 + +0.0 5,718,598 - -0.4 5,718,607 + +0.0 5,718,607 + -4.2 5,718,608 - +0.0 5,718,790 - Pf1N1B4_5139 0.12 -1.8 5,718,790 - Pf1N1B4_5139 0.12 +0.0 5,718,950 + Pf1N1B4_5139 0.22 +0.0 5,719,089 + Pf1N1B4_5139 0.32 -2.0 5,719,091 + Pf1N1B4_5139 0.32 +0.0 5,719,091 + +0.0 5,719,092 - Pf1N1B4_5139 0.32 -2.4 5,719,141 + Pf1N1B4_5139 0.35 -1.1 5,719,148 - Pf1N1B4_5139 0.36 +0.0
Or see this region's nucleotide sequence