Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5085 and Pf1N1B4_5086 are separated by 29 nucleotides Pf1N1B4_5086 and Pf1N1B4_5087 are separated by 23 nucleotides Pf1N1B4_5087 and Pf1N1B4_5088 overlap by 4 nucleotides
Pf1N1B4_5085 - Capsular polysaccharide synthesis enzyme CpsA, sugar transferase, at 5,635,163 to 5,636,554
_5085
Pf1N1B4_5086 - N-acetylmannosaminyltransferase (EC 2.4.1.187), at 5,636,584 to 5,637,291
_5086
Pf1N1B4_5087 - Glycosyl transferase, group 1 family protein, at 5,637,315 to 5,638,394
_5087
Pf1N1B4_5088 - Glycosyl transferase, group 1 family protein, at 5,638,391 to 5,639,503
_5088
Position (kb) 5637
5638
5639 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5636.355 kb on - strand, within Pf1N1B4_5085 at 5636.471 kb on + strand at 5636.549 kb on + strand at 5636.549 kb on + strand at 5636.550 kb on - strand at 5636.612 kb on - strand at 5637.127 kb on + strand, within Pf1N1B4_5086 at 5637.420 kb on + strand at 5637.608 kb on + strand, within Pf1N1B4_5087 at 5637.627 kb on + strand, within Pf1N1B4_5087 at 5637.627 kb on + strand, within Pf1N1B4_5087 at 5637.627 kb on + strand, within Pf1N1B4_5087 at 5637.627 kb on + strand, within Pf1N1B4_5087 at 5637.628 kb on - strand, within Pf1N1B4_5087 at 5637.628 kb on - strand, within Pf1N1B4_5087 at 5637.702 kb on + strand, within Pf1N1B4_5087 at 5637.702 kb on + strand, within Pf1N1B4_5087 at 5637.703 kb on - strand, within Pf1N1B4_5087 at 5637.703 kb on - strand, within Pf1N1B4_5087 at 5637.899 kb on - strand, within Pf1N1B4_5087 at 5637.934 kb on - strand, within Pf1N1B4_5087 at 5638.345 kb on - strand at 5638.345 kb on - strand at 5638.372 kb on - strand at 5638.372 kb on - strand at 5638.374 kb on + strand at 5638.995 kb on + strand, within Pf1N1B4_5088
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 5,636,355 - Pf1N1B4_5085 0.86 +0.0 5,636,471 + +0.0 5,636,549 + -2.0 5,636,549 + -2.5 5,636,550 - +0.0 5,636,612 - +0.0 5,637,127 + Pf1N1B4_5086 0.77 -1.6 5,637,420 + +0.0 5,637,608 + Pf1N1B4_5087 0.27 -2.0 5,637,627 + Pf1N1B4_5087 0.29 -2.3 5,637,627 + Pf1N1B4_5087 0.29 +0.0 5,637,627 + Pf1N1B4_5087 0.29 +0.0 5,637,627 + Pf1N1B4_5087 0.29 +0.0 5,637,628 - Pf1N1B4_5087 0.29 +0.0 5,637,628 - Pf1N1B4_5087 0.29 +0.0 5,637,702 + Pf1N1B4_5087 0.36 -1.2 5,637,702 + Pf1N1B4_5087 0.36 -0.9 5,637,703 - Pf1N1B4_5087 0.36 +0.0 5,637,703 - Pf1N1B4_5087 0.36 -3.0 5,637,899 - Pf1N1B4_5087 0.54 +0.0 5,637,934 - Pf1N1B4_5087 0.57 +0.0 5,638,345 - -3.0 5,638,345 - +0.0 5,638,372 - -1.0 5,638,372 - -0.0 5,638,374 + -1.6 5,638,995 + Pf1N1B4_5088 0.54 +0.0
Or see this region's nucleotide sequence