Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4775 and Pf1N1B4_4776 are separated by 194 nucleotides Pf1N1B4_4776 and Pf1N1B4_4777 are separated by 325 nucleotides
Pf1N1B4_4775 - Sensory histidine kinase QseC, at 5,276,612 to 5,277,913
_4775
Pf1N1B4_4776 - Nicotinamidase (EC 3.5.1.19), at 5,278,108 to 5,278,746
_4776
Pf1N1B4_4777 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 5,279,072 to 5,279,884
_4777
Position (kb) 5278
5279 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5277.422 kb on + strand, within Pf1N1B4_4775 at 5277.546 kb on + strand, within Pf1N1B4_4775 at 5277.546 kb on + strand, within Pf1N1B4_4775 at 5277.943 kb on + strand at 5277.991 kb on - strand at 5277.991 kb on - strand at 5278.127 kb on - strand at 5278.307 kb on + strand, within Pf1N1B4_4776 at 5278.307 kb on + strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.388 kb on + strand, within Pf1N1B4_4776 at 5278.477 kb on - strand, within Pf1N1B4_4776 at 5278.539 kb on - strand at 5278.705 kb on + strand at 5278.705 kb on + strand at 5278.706 kb on - strand at 5278.706 kb on - strand at 5278.783 kb on - strand at 5278.783 kb on - strand at 5278.788 kb on + strand at 5278.788 kb on + strand at 5278.789 kb on - strand at 5278.899 kb on + strand at 5279.031 kb on + strand at 5279.032 kb on - strand at 5279.032 kb on - strand at 5279.045 kb on + strand at 5279.046 kb on - strand at 5279.046 kb on - strand at 5279.046 kb on - strand at 5279.082 kb on - strand at 5279.530 kb on - strand, within Pf1N1B4_4777 at 5279.530 kb on - strand, within Pf1N1B4_4777 at 5279.588 kb on + strand, within Pf1N1B4_4777 at 5279.649 kb on - strand, within Pf1N1B4_4777
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 5,277,422 + Pf1N1B4_4775 0.62 -3.7 5,277,546 + Pf1N1B4_4775 0.72 +0.0 5,277,546 + Pf1N1B4_4775 0.72 +0.0 5,277,943 + +0.0 5,277,991 - -2.8 5,277,991 - +0.0 5,278,127 - -2.1 5,278,307 + Pf1N1B4_4776 0.31 +0.0 5,278,307 + Pf1N1B4_4776 0.31 +0.0 5,278,308 - Pf1N1B4_4776 0.31 +0.0 5,278,308 - Pf1N1B4_4776 0.31 +0.0 5,278,308 - Pf1N1B4_4776 0.31 +0.0 5,278,308 - Pf1N1B4_4776 0.31 +0.0 5,278,388 + Pf1N1B4_4776 0.44 +0.0 5,278,477 - Pf1N1B4_4776 0.58 +0.0 5,278,539 - +0.0 5,278,705 + -2.3 5,278,705 + +0.0 5,278,706 - -3.2 5,278,706 - -2.8 5,278,783 - +0.0 5,278,783 - -2.4 5,278,788 + -2.2 5,278,788 + +0.0 5,278,789 - +0.0 5,278,899 + -1.2 5,279,031 + +0.0 5,279,032 - -5.4 5,279,032 - -2.8 5,279,045 + +0.0 5,279,046 - -2.1 5,279,046 - -1.4 5,279,046 - +0.0 5,279,082 - +0.0 5,279,530 - Pf1N1B4_4777 0.56 +0.0 5,279,530 - Pf1N1B4_4777 0.56 -2.2 5,279,588 + Pf1N1B4_4777 0.63 +0.0 5,279,649 - Pf1N1B4_4777 0.71 +0.0
Or see this region's nucleotide sequence