Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4740

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4738 and Pf1N1B4_4739 overlap by 4 nucleotidesPf1N1B4_4739 and Pf1N1B4_4740 are separated by 231 nucleotidesPf1N1B4_4740 and Pf1N1B4_4741 are separated by 292 nucleotides Pf1N1B4_4738 - Ribosylnicotinamide kinase (EC 2.7.1.22), at 5,235,500 to 5,236,024 _4738 Pf1N1B4_4739 - Ribosyl nicotinamide transporter, PnuC-like, at 5,236,021 to 5,236,584 _4739 Pf1N1B4_4740 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 5,236,816 to 5,238,450 _4740 Pf1N1B4_4741 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 5,238,743 to 5,239,573 _4741 Position (kb) 5236 5237 5238 5239Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 5235.914 kb on - strand, within Pf1N1B4_4738at 5235.927 kb on + strand, within Pf1N1B4_4738at 5236.109 kb on + strand, within Pf1N1B4_4739at 5236.109 kb on + strand, within Pf1N1B4_4739at 5236.122 kb on - strand, within Pf1N1B4_4739at 5236.122 kb on - strand, within Pf1N1B4_4739at 5236.400 kb on + strand, within Pf1N1B4_4739at 5236.400 kb on + strand, within Pf1N1B4_4739at 5236.400 kb on + strand, within Pf1N1B4_4739at 5236.401 kb on - strand, within Pf1N1B4_4739at 5236.418 kb on + strand, within Pf1N1B4_4739at 5236.419 kb on - strand, within Pf1N1B4_4739at 5236.473 kb on + strand, within Pf1N1B4_4739at 5236.474 kb on - strand, within Pf1N1B4_4739at 5236.474 kb on - strandat 5236.474 kb on - strand, within Pf1N1B4_4739at 5236.474 kb on - strand, within Pf1N1B4_4739at 5236.475 kb on + strand, within Pf1N1B4_4739at 5236.476 kb on - strand, within Pf1N1B4_4739at 5236.756 kb on + strandat 5237.184 kb on + strand, within Pf1N1B4_4740at 5237.513 kb on + strand, within Pf1N1B4_4740at 5237.919 kb on - strand, within Pf1N1B4_4740at 5237.919 kb on - strand, within Pf1N1B4_4740at 5237.966 kb on + strandat 5237.966 kb on + strand, within Pf1N1B4_4740at 5238.379 kb on + strandat 5238.379 kb on + strandat 5238.480 kb on + strandat 5238.541 kb on + strandat 5238.687 kb on - strandat 5238.687 kb on - strandat 5238.693 kb on + strandat 5238.693 kb on + strandat 5238.836 kb on - strand, within Pf1N1B4_4741at 5238.883 kb on + strand, within Pf1N1B4_4741at 5238.883 kb on + strandat 5238.886 kb on + strand, within Pf1N1B4_4741at 5238.886 kb on + strandat 5239.072 kb on + strand, within Pf1N1B4_4741at 5239.174 kb on + strand, within Pf1N1B4_4741at 5239.174 kb on + strand, within Pf1N1B4_4741at 5239.174 kb on + strandat 5239.346 kb on + strand, within Pf1N1B4_4741at 5239.355 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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5,235,914 - Pf1N1B4_4738 0.79 +0.0
5,235,927 + Pf1N1B4_4738 0.81 +0.0
5,236,109 + Pf1N1B4_4739 0.16 -2.0
5,236,109 + Pf1N1B4_4739 0.16 -2.9
5,236,122 - Pf1N1B4_4739 0.18 +0.0
5,236,122 - Pf1N1B4_4739 0.18 +0.0
5,236,400 + Pf1N1B4_4739 0.67 -4.9
5,236,400 + Pf1N1B4_4739 0.67 -3.0
5,236,400 + Pf1N1B4_4739 0.67 -2.7
5,236,401 - Pf1N1B4_4739 0.67 -2.6
5,236,418 + Pf1N1B4_4739 0.70 +0.0
5,236,419 - Pf1N1B4_4739 0.71 +0.0
5,236,473 + Pf1N1B4_4739 0.80 -3.8
5,236,474 - Pf1N1B4_4739 0.80 +0.0
5,236,474 - -3.3
5,236,474 - Pf1N1B4_4739 0.80 -3.1
5,236,474 - Pf1N1B4_4739 0.80 -2.8
5,236,475 + Pf1N1B4_4739 0.80 +0.0
5,236,476 - Pf1N1B4_4739 0.81 +0.0
5,236,756 + -1.8
5,237,184 + Pf1N1B4_4740 0.23 -2.7
5,237,513 + Pf1N1B4_4740 0.43 +0.0
5,237,919 - Pf1N1B4_4740 0.67 -3.9
5,237,919 - Pf1N1B4_4740 0.67 +0.0
5,237,966 + -1.2
5,237,966 + Pf1N1B4_4740 0.70 +0.0
5,238,379 + -1.5
5,238,379 + +0.0
5,238,480 + -2.3
5,238,541 + -1.0
5,238,687 - -4.1
5,238,687 - +0.0
5,238,693 + -3.1
5,238,693 + -1.7
5,238,836 - Pf1N1B4_4741 0.11 -2.1
5,238,883 + Pf1N1B4_4741 0.17 +0.0
5,238,883 + +0.0
5,238,886 + Pf1N1B4_4741 0.17 +0.0
5,238,886 + -1.2
5,239,072 + Pf1N1B4_4741 0.40 +0.0
5,239,174 + Pf1N1B4_4741 0.52 -2.1
5,239,174 + Pf1N1B4_4741 0.52 +0.0
5,239,174 + -3.2
5,239,346 + Pf1N1B4_4741 0.73 -1.5
5,239,355 + +0.0

Or see this region's nucleotide sequence