Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4738 and Pf1N1B4_4739 overlap by 4 nucleotides Pf1N1B4_4739 and Pf1N1B4_4740 are separated by 231 nucleotides
Pf1N1B4_4738 - Ribosylnicotinamide kinase (EC 2.7.1.22), at 5,235,500 to 5,236,024
_4738
Pf1N1B4_4739 - Ribosyl nicotinamide transporter, PnuC-like, at 5,236,021 to 5,236,584
_4739
Pf1N1B4_4740 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 5,236,816 to 5,238,450
_4740
Position (kb) 5236
5237 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5235.233 kb on + strand at 5235.233 kb on + strand at 5235.233 kb on + strand at 5235.234 kb on - strand at 5235.244 kb on + strand at 5235.353 kb on + strand at 5235.353 kb on + strand at 5235.439 kb on + strand at 5235.712 kb on - strand, within Pf1N1B4_4738 at 5235.914 kb on - strand, within Pf1N1B4_4738 at 5235.927 kb on + strand, within Pf1N1B4_4738 at 5236.109 kb on + strand, within Pf1N1B4_4739 at 5236.109 kb on + strand, within Pf1N1B4_4739 at 5236.122 kb on - strand, within Pf1N1B4_4739 at 5236.122 kb on - strand, within Pf1N1B4_4739 at 5236.400 kb on + strand, within Pf1N1B4_4739 at 5236.400 kb on + strand, within Pf1N1B4_4739 at 5236.400 kb on + strand, within Pf1N1B4_4739 at 5236.401 kb on - strand, within Pf1N1B4_4739 at 5236.418 kb on + strand, within Pf1N1B4_4739 at 5236.419 kb on - strand, within Pf1N1B4_4739 at 5236.473 kb on + strand, within Pf1N1B4_4739 at 5236.474 kb on - strand, within Pf1N1B4_4739 at 5236.474 kb on - strand at 5236.474 kb on - strand, within Pf1N1B4_4739 at 5236.474 kb on - strand, within Pf1N1B4_4739 at 5236.475 kb on + strand, within Pf1N1B4_4739 at 5236.476 kb on - strand, within Pf1N1B4_4739 at 5236.756 kb on + strand at 5237.184 kb on + strand, within Pf1N1B4_4740 at 5237.513 kb on + strand, within Pf1N1B4_4740
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 5,235,233 + +0.0 5,235,233 + +0.0 5,235,233 + +0.0 5,235,234 - +0.0 5,235,244 + -2.7 5,235,353 + +0.0 5,235,353 + +0.0 5,235,439 + +0.0 5,235,712 - Pf1N1B4_4738 0.40 +0.0 5,235,914 - Pf1N1B4_4738 0.79 +0.0 5,235,927 + Pf1N1B4_4738 0.81 +0.0 5,236,109 + Pf1N1B4_4739 0.16 -2.0 5,236,109 + Pf1N1B4_4739 0.16 -2.9 5,236,122 - Pf1N1B4_4739 0.18 +0.0 5,236,122 - Pf1N1B4_4739 0.18 +0.0 5,236,400 + Pf1N1B4_4739 0.67 -4.9 5,236,400 + Pf1N1B4_4739 0.67 -3.0 5,236,400 + Pf1N1B4_4739 0.67 -2.7 5,236,401 - Pf1N1B4_4739 0.67 -2.6 5,236,418 + Pf1N1B4_4739 0.70 +0.0 5,236,419 - Pf1N1B4_4739 0.71 +0.0 5,236,473 + Pf1N1B4_4739 0.80 -3.8 5,236,474 - Pf1N1B4_4739 0.80 +0.0 5,236,474 - -3.3 5,236,474 - Pf1N1B4_4739 0.80 -3.1 5,236,474 - Pf1N1B4_4739 0.80 -2.8 5,236,475 + Pf1N1B4_4739 0.80 +0.0 5,236,476 - Pf1N1B4_4739 0.81 +0.0 5,236,756 + -1.8 5,237,184 + Pf1N1B4_4740 0.23 -2.7 5,237,513 + Pf1N1B4_4740 0.43 +0.0
Or see this region's nucleotide sequence