Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4733 and Pf1N1B4_4734 are separated by 32 nucleotides Pf1N1B4_4734 and Pf1N1B4_4735 are separated by 62 nucleotides Pf1N1B4_4735 and Pf1N1B4_4736 are separated by 51 nucleotides Pf1N1B4_4736 and Pf1N1B4_4737 are separated by 1 nucleotides
Pf1N1B4_4733 - Fap system putative outer membrane protein, at 5,231,152 to 5,231,889
_4733
Pf1N1B4_4734 - Fap protein with C39 domain, at 5,231,922 to 5,232,602
_4734
Pf1N1B4_4735 - Fap amyloid fibril major component, at 5,232,665 to 5,233,954
_4735
Pf1N1B4_4736 - Fap amyloid fibril minor component, at 5,234,006 to 5,234,578
_4736
Pf1N1B4_4737 - hypothetical protein, at 5,234,580 to 5,234,801
_4737
Position (kb) 5232
5233
5234 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5231.698 kb on + strand, within Pf1N1B4_4733 at 5231.698 kb on + strand, within Pf1N1B4_4733 at 5232.145 kb on + strand at 5232.145 kb on + strand, within Pf1N1B4_4734 at 5232.146 kb on - strand, within Pf1N1B4_4734 at 5232.213 kb on + strand, within Pf1N1B4_4734 at 5232.214 kb on - strand, within Pf1N1B4_4734 at 5232.397 kb on + strand, within Pf1N1B4_4734 at 5232.411 kb on + strand at 5232.476 kb on + strand, within Pf1N1B4_4734 at 5232.487 kb on - strand, within Pf1N1B4_4734 at 5232.505 kb on + strand, within Pf1N1B4_4734 at 5232.505 kb on + strand, within Pf1N1B4_4734 at 5232.572 kb on - strand at 5232.572 kb on - strand at 5232.831 kb on + strand, within Pf1N1B4_4735 at 5232.831 kb on + strand at 5232.831 kb on + strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.867 kb on + strand at 5232.868 kb on - strand, within Pf1N1B4_4735 at 5232.904 kb on + strand, within Pf1N1B4_4735 at 5232.953 kb on - strand, within Pf1N1B4_4735 at 5233.035 kb on - strand, within Pf1N1B4_4735 at 5233.035 kb on - strand, within Pf1N1B4_4735 at 5233.349 kb on - strand, within Pf1N1B4_4735 at 5233.815 kb on + strand, within Pf1N1B4_4735 at 5233.847 kb on - strand at 5233.889 kb on + strand at 5233.890 kb on - strand at 5233.968 kb on - strand at 5233.971 kb on + strand at 5233.971 kb on + strand at 5233.971 kb on + strand at 5233.982 kb on - strand at 5233.994 kb on + strand at 5234.021 kb on + strand at 5234.218 kb on + strand, within Pf1N1B4_4736 at 5234.219 kb on - strand, within Pf1N1B4_4736 at 5234.224 kb on + strand, within Pf1N1B4_4736 at 5234.224 kb on + strand, within Pf1N1B4_4736 at 5234.225 kb on - strand, within Pf1N1B4_4736 at 5234.228 kb on + strand, within Pf1N1B4_4736 at 5234.735 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 5,231,698 + Pf1N1B4_4733 0.74 -1.7 5,231,698 + Pf1N1B4_4733 0.74 -1.0 5,232,145 + +0.0 5,232,145 + Pf1N1B4_4734 0.33 +0.0 5,232,146 - Pf1N1B4_4734 0.33 -2.4 5,232,213 + Pf1N1B4_4734 0.43 +0.0 5,232,214 - Pf1N1B4_4734 0.43 +0.0 5,232,397 + Pf1N1B4_4734 0.70 -2.3 5,232,411 + +0.0 5,232,476 + Pf1N1B4_4734 0.81 +0.0 5,232,487 - Pf1N1B4_4734 0.83 -1.8 5,232,505 + Pf1N1B4_4734 0.86 +0.0 5,232,505 + Pf1N1B4_4734 0.86 -2.1 5,232,572 - -1.5 5,232,572 - -2.6 5,232,831 + Pf1N1B4_4735 0.13 -3.0 5,232,831 + +0.0 5,232,831 + Pf1N1B4_4735 0.13 +0.0 5,232,832 - Pf1N1B4_4735 0.13 +0.0 5,232,832 - Pf1N1B4_4735 0.13 +0.0 5,232,832 - Pf1N1B4_4735 0.13 -0.9 5,232,867 + -1.4 5,232,868 - Pf1N1B4_4735 0.16 +0.0 5,232,904 + Pf1N1B4_4735 0.19 -1.2 5,232,953 - Pf1N1B4_4735 0.22 -3.7 5,233,035 - Pf1N1B4_4735 0.29 +0.0 5,233,035 - Pf1N1B4_4735 0.29 -1.3 5,233,349 - Pf1N1B4_4735 0.53 +0.0 5,233,815 + Pf1N1B4_4735 0.89 +0.0 5,233,847 - -1.4 5,233,889 + +0.0 5,233,890 - -1.8 5,233,968 - -1.6 5,233,971 + +0.0 5,233,971 + +0.0 5,233,971 + -1.5 5,233,982 - +0.0 5,233,994 + +0.0 5,234,021 + +0.0 5,234,218 + Pf1N1B4_4736 0.37 -1.9 5,234,219 - Pf1N1B4_4736 0.37 -4.1 5,234,224 + Pf1N1B4_4736 0.38 -3.6 5,234,224 + Pf1N1B4_4736 0.38 +0.0 5,234,225 - Pf1N1B4_4736 0.38 +0.0 5,234,228 + Pf1N1B4_4736 0.39 -2.2 5,234,735 + -1.6
Or see this region's nucleotide sequence