Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_47

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_45 and Pf1N1B4_46 are separated by 55 nucleotidesPf1N1B4_46 and Pf1N1B4_47 are separated by 3 nucleotidesPf1N1B4_47 and Pf1N1B4_48 are separated by 145 nucleotidesPf1N1B4_48 and Pf1N1B4_49 are separated by 30 nucleotidesPf1N1B4_49 and Pf1N1B4_50 are separated by 163 nucleotides Pf1N1B4_45 - Copper resistance protein B, at 53,588 to 54,478 _45 Pf1N1B4_46 - Copper resistance protein C precursor, at 54,534 to 54,917 _46 Pf1N1B4_47 - Copper resistance protein D, at 54,921 to 55,850 _47 Pf1N1B4_48 - hypothetical protein, at 55,996 to 56,406 _48 Pf1N1B4_49 - hypothetical protein, at 56,437 to 56,643 _49 Pf1N1B4_50 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 56,807 to 56,989 _50 Position (kb) 54 55 56Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 54.137 kb on + strand, within Pf1N1B4_45at 54.138 kb on - strand, within Pf1N1B4_45at 54.210 kb on - strand, within Pf1N1B4_45at 54.365 kb on + strandat 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.365 kb on + strand, within Pf1N1B4_45at 54.366 kb on - strand, within Pf1N1B4_45at 54.366 kb on - strand, within Pf1N1B4_45at 54.366 kb on - strand, within Pf1N1B4_45at 54.393 kb on - strandat 54.410 kb on + strandat 54.411 kb on - strandat 54.485 kb on + strandat 54.486 kb on - strandat 54.514 kb on - strandat 54.843 kb on + strand, within Pf1N1B4_46at 54.843 kb on + strand, within Pf1N1B4_46at 54.844 kb on - strand, within Pf1N1B4_46at 54.844 kb on - strand, within Pf1N1B4_46at 54.844 kb on - strand, within Pf1N1B4_46at 54.844 kb on - strand, within Pf1N1B4_46at 54.899 kb on + strandat 54.899 kb on + strandat 54.899 kb on + strandat 54.899 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.960 kb on + strandat 54.961 kb on - strandat 54.961 kb on - strandat 54.961 kb on - strandat 54.961 kb on - strandat 54.961 kb on - strandat 55.006 kb on - strandat 55.006 kb on - strandat 55.014 kb on - strand, within Pf1N1B4_47at 55.276 kb on + strand, within Pf1N1B4_47at 55.277 kb on - strand, within Pf1N1B4_47at 55.341 kb on - strand, within Pf1N1B4_47at 55.499 kb on + strand, within Pf1N1B4_47at 55.611 kb on + strand, within Pf1N1B4_47at 55.611 kb on + strand, within Pf1N1B4_47at 55.611 kb on + strand, within Pf1N1B4_47at 55.611 kb on + strand, within Pf1N1B4_47at 55.612 kb on - strand, within Pf1N1B4_47at 55.848 kb on + strandat 55.848 kb on + strandat 55.863 kb on + strandat 55.863 kb on + strandat 55.864 kb on - strandat 55.870 kb on + strandat 55.980 kb on + strandat 55.980 kb on + strandat 55.981 kb on - strandat 56.005 kb on + strandat 56.006 kb on - strandat 56.516 kb on + strand, within Pf1N1B4_49at 56.517 kb on - strand, within Pf1N1B4_49at 56.520 kb on - strand, within Pf1N1B4_49at 56.559 kb on + strand, within Pf1N1B4_49at 56.559 kb on + strand, within Pf1N1B4_49at 56.560 kb on - strand, within Pf1N1B4_49at 56.560 kb on - strandat 56.560 kb on - strand, within Pf1N1B4_49at 56.588 kb on + strand, within Pf1N1B4_49at 56.588 kb on + strand, within Pf1N1B4_49at 56.767 kb on + strandat 56.784 kb on + strandat 56.837 kb on + strand, within Pf1N1B4_50at 56.837 kb on + strand, within Pf1N1B4_50at 56.837 kb on + strand, within Pf1N1B4_50at 56.837 kb on + strand, within Pf1N1B4_50at 56.838 kb on - strand, within Pf1N1B4_50at 56.838 kb on - strand, within Pf1N1B4_50at 56.838 kb on - strand, within Pf1N1B4_50at 56.838 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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54,137 + Pf1N1B4_45 0.62 -2.4
54,138 - Pf1N1B4_45 0.62 +0.0
54,210 - Pf1N1B4_45 0.70 -1.0
54,365 + -1.3
54,365 + Pf1N1B4_45 0.87 +0.0
54,365 + Pf1N1B4_45 0.87 -1.5
54,365 + Pf1N1B4_45 0.87 +0.0
54,365 + Pf1N1B4_45 0.87 +0.0
54,365 + Pf1N1B4_45 0.87 +0.0
54,365 + Pf1N1B4_45 0.87 -1.2
54,366 - Pf1N1B4_45 0.87 +0.0
54,366 - Pf1N1B4_45 0.87 -0.8
54,366 - Pf1N1B4_45 0.87 -1.2
54,393 - +0.0
54,410 + -2.3
54,411 - +0.0
54,485 + -1.3
54,486 - +0.0
54,514 - -4.2
54,843 + Pf1N1B4_46 0.80 +0.0
54,843 + Pf1N1B4_46 0.80 +0.0
54,844 - Pf1N1B4_46 0.81 -2.5
54,844 - Pf1N1B4_46 0.81 -2.3
54,844 - Pf1N1B4_46 0.81 +0.0
54,844 - Pf1N1B4_46 0.81 +0.0
54,899 + +0.0
54,899 + +0.0
54,899 + +0.0
54,899 + -1.1
54,960 + +0.0
54,960 + +0.0
54,960 + -0.4
54,960 + -1.2
54,960 + +0.0
54,960 + +0.0
54,960 + -1.3
54,961 - -2.0
54,961 - +0.0
54,961 - -2.4
54,961 - +0.0
54,961 - +0.0
55,006 - -4.0
55,006 - +0.0
55,014 - Pf1N1B4_47 0.10 +0.0
55,276 + Pf1N1B4_47 0.38 +0.0
55,277 - Pf1N1B4_47 0.38 +0.0
55,341 - Pf1N1B4_47 0.45 +0.0
55,499 + Pf1N1B4_47 0.62 +0.0
55,611 + Pf1N1B4_47 0.74 +0.0
55,611 + Pf1N1B4_47 0.74 +0.3
55,611 + Pf1N1B4_47 0.74 -3.2
55,611 + Pf1N1B4_47 0.74 -1.7
55,612 - Pf1N1B4_47 0.74 +0.0
55,848 + +0.0
55,848 + +0.0
55,863 + +0.0
55,863 + +0.0
55,864 - +0.0
55,870 + +0.0
55,980 + +0.0
55,980 + -2.8
55,981 - +0.0
56,005 + +0.0
56,006 - +0.0
56,516 + Pf1N1B4_49 0.38 +0.0
56,517 - Pf1N1B4_49 0.39 +0.0
56,520 - Pf1N1B4_49 0.40 +0.0
56,559 + Pf1N1B4_49 0.59 -2.6
56,559 + Pf1N1B4_49 0.59 +0.0
56,560 - Pf1N1B4_49 0.59 +0.0
56,560 - +0.0
56,560 - Pf1N1B4_49 0.59 +0.0
56,588 + Pf1N1B4_49 0.73 -1.1
56,588 + Pf1N1B4_49 0.73 -2.3
56,767 + -2.0
56,784 + +0.0
56,837 + Pf1N1B4_50 0.16 +0.0
56,837 + Pf1N1B4_50 0.16 +0.0
56,837 + Pf1N1B4_50 0.16 -2.6
56,837 + Pf1N1B4_50 0.16 +0.0
56,838 - Pf1N1B4_50 0.17 +0.0
56,838 - Pf1N1B4_50 0.17 +0.0
56,838 - Pf1N1B4_50 0.17 -2.8
56,838 - -3.1

Or see this region's nucleotide sequence