Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4672 and Pf1N1B4_4673 are separated by 186 nucleotides Pf1N1B4_4673 and Pf1N1B4_4674 are separated by 252 nucleotides Pf1N1B4_4674 and Pf1N1B4_4675 are separated by 161 nucleotides
Pf1N1B4_4672 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 5,153,126 to 5,154,535
_4672
Pf1N1B4_4673 - 4Fe-4S ferredoxin, iron-sulfur binding, at 5,154,722 to 5,156,089
_4673
Pf1N1B4_4674 - hypothetical protein, at 5,156,342 to 5,156,575
_4674
Pf1N1B4_4675 - Mg(2+) transport ATPase protein C, at 5,156,737 to 5,157,210
_4675
Position (kb) 5154
5155
5156
5157 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5153.978 kb on + strand, within Pf1N1B4_4672 at 5153.979 kb on - strand, within Pf1N1B4_4672 at 5154.393 kb on + strand, within Pf1N1B4_4672 at 5154.396 kb on + strand at 5154.396 kb on + strand at 5154.396 kb on + strand at 5154.716 kb on - strand at 5154.984 kb on + strand at 5154.984 kb on + strand, within Pf1N1B4_4673 at 5154.985 kb on - strand, within Pf1N1B4_4673 at 5154.985 kb on - strand, within Pf1N1B4_4673 at 5154.985 kb on - strand, within Pf1N1B4_4673 at 5155.179 kb on + strand at 5155.180 kb on - strand, within Pf1N1B4_4673 at 5155.506 kb on + strand, within Pf1N1B4_4673 at 5155.506 kb on + strand, within Pf1N1B4_4673 at 5155.507 kb on - strand, within Pf1N1B4_4673 at 5155.507 kb on - strand, within Pf1N1B4_4673 at 5155.603 kb on - strand, within Pf1N1B4_4673 at 5155.704 kb on - strand, within Pf1N1B4_4673 at 5156.101 kb on + strand at 5156.101 kb on + strand at 5156.185 kb on - strand at 5156.185 kb on - strand at 5156.305 kb on - strand at 5156.436 kb on + strand, within Pf1N1B4_4674 at 5156.669 kb on + strand at 5156.669 kb on + strand at 5156.669 kb on + strand at 5156.669 kb on + strand at 5156.669 kb on + strand at 5156.669 kb on + strand at 5156.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 5,153,978 + Pf1N1B4_4672 0.60 +0.0 5,153,979 - Pf1N1B4_4672 0.60 +0.5 5,154,393 + Pf1N1B4_4672 0.90 +0.0 5,154,396 + +0.4 5,154,396 + -2.3 5,154,396 + +0.7 5,154,716 - -0.9 5,154,984 + +1.5 5,154,984 + Pf1N1B4_4673 0.19 +0.0 5,154,985 - Pf1N1B4_4673 0.19 +0.0 5,154,985 - Pf1N1B4_4673 0.19 +0.0 5,154,985 - Pf1N1B4_4673 0.19 +2.2 5,155,179 + +3.5 5,155,180 - Pf1N1B4_4673 0.33 +0.0 5,155,506 + Pf1N1B4_4673 0.57 +1.1 5,155,506 + Pf1N1B4_4673 0.57 +1.6 5,155,507 - Pf1N1B4_4673 0.57 +0.0 5,155,507 - Pf1N1B4_4673 0.57 +0.0 5,155,603 - Pf1N1B4_4673 0.64 +0.0 5,155,704 - Pf1N1B4_4673 0.72 +0.0 5,156,101 + +0.0 5,156,101 + +0.0 5,156,185 - +0.0 5,156,185 - +2.5 5,156,305 - +0.0 5,156,436 + Pf1N1B4_4674 0.40 -3.9 5,156,669 + -2.1 5,156,669 + -4.0 5,156,669 + -1.9 5,156,669 + +0.0 5,156,669 + -3.3 5,156,669 + +0.0 5,156,670 - +0.0
Or see this region's nucleotide sequence