Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4592

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4591 and Pf1N1B4_4592 are separated by 1 nucleotidesPf1N1B4_4592 and Pf1N1B4_4593 are separated by 4 nucleotides Pf1N1B4_4591 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 5,061,902 to 5,062,921 _4591 Pf1N1B4_4592 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 5,062,923 to 5,063,996 _4592 Pf1N1B4_4593 - ABC transporter, periplasmic substrate-binding protein, at 5,064,001 to 5,065,842 _4593 Position (kb) 5062 5063 5064Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5062.221 kb on + strand, within Pf1N1B4_4591at 5062.603 kb on - strandat 5062.970 kb on - strandat 5063.249 kb on - strand, within Pf1N1B4_4592at 5063.354 kb on - strand, within Pf1N1B4_4592at 5063.746 kb on + strand, within Pf1N1B4_4592at 5063.747 kb on - strand, within Pf1N1B4_4592at 5064.098 kb on + strandat 5064.099 kb on - strandat 5064.099 kb on - strandat 5064.179 kb on + strandat 5064.183 kb on - strandat 5064.614 kb on + strand, within Pf1N1B4_4593at 5064.983 kb on + strand, within Pf1N1B4_4593at 5064.984 kb on - strand, within Pf1N1B4_4593at 5064.984 kb on - strand, within Pf1N1B4_4593at 5064.984 kb on - strand, within Pf1N1B4_4593

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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5,062,221 + Pf1N1B4_4591 0.31 +0.1
5,062,603 - +0.1
5,062,970 - +0.1
5,063,249 - Pf1N1B4_4592 0.30 +0.1
5,063,354 - Pf1N1B4_4592 0.40 +0.1
5,063,746 + Pf1N1B4_4592 0.77 -4.0
5,063,747 - Pf1N1B4_4592 0.77 -1.3
5,064,098 + +0.1
5,064,099 - +0.1
5,064,099 - +0.1
5,064,179 + -0.7
5,064,183 - -0.9
5,064,614 + Pf1N1B4_4593 0.33 -3.1
5,064,983 + Pf1N1B4_4593 0.53 +0.1
5,064,984 - Pf1N1B4_4593 0.53 +0.1
5,064,984 - Pf1N1B4_4593 0.53 -0.6
5,064,984 - Pf1N1B4_4593 0.53 +0.1

Or see this region's nucleotide sequence