Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4520 and Pf1N1B4_4521 are separated by 2 nucleotides Pf1N1B4_4521 and Pf1N1B4_4522 are separated by 140 nucleotides Pf1N1B4_4522 and Pf1N1B4_4523 are separated by 3 nucleotides
Pf1N1B4_4520 - GENE II AND X PROTEINS, at 4,984,635 to 4,985,048
_4520
Pf1N1B4_4521 - Probable transmembrane protein, at 4,985,051 to 4,985,485
_4521
Pf1N1B4_4522 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 4,985,626 to 4,986,060
_4522
Pf1N1B4_4523 - RNA polymerase sigma-70 factor, ECF subfamily, at 4,986,064 to 4,986,957
_4523
Position (kb)
4985
4986
4987 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4985.265 kb on - strand, within Pf1N1B4_4521 at 4985.269 kb on - strand at 4985.269 kb on - strand, within Pf1N1B4_4521 at 4985.269 kb on - strand, within Pf1N1B4_4521 at 4985.269 kb on - strand, within Pf1N1B4_4521 at 4985.300 kb on + strand, within Pf1N1B4_4521 at 4985.301 kb on - strand, within Pf1N1B4_4521 at 4985.518 kb on - strand at 4985.966 kb on + strand, within Pf1N1B4_4522 at 4985.966 kb on + strand, within Pf1N1B4_4522 at 4986.285 kb on + strand, within Pf1N1B4_4523 at 4986.285 kb on + strand, within Pf1N1B4_4523 at 4986.301 kb on + strand, within Pf1N1B4_4523 at 4986.777 kb on + strand, within Pf1N1B4_4523 at 4986.778 kb on - strand, within Pf1N1B4_4523 at 4986.778 kb on - strand, within Pf1N1B4_4523 at 4986.956 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 4,985,265 - Pf1N1B4_4521 0.49 +0.1 4,985,269 - -3.3 4,985,269 - Pf1N1B4_4521 0.50 -2.9 4,985,269 - Pf1N1B4_4521 0.50 -1.8 4,985,269 - Pf1N1B4_4521 0.50 +0.1 4,985,300 + Pf1N1B4_4521 0.57 +0.1 4,985,301 - Pf1N1B4_4521 0.57 -2.0 4,985,518 - -2.2 4,985,966 + Pf1N1B4_4522 0.78 -2.8 4,985,966 + Pf1N1B4_4522 0.78 -2.8 4,986,285 + Pf1N1B4_4523 0.25 +0.1 4,986,285 + Pf1N1B4_4523 0.25 +0.1 4,986,301 + Pf1N1B4_4523 0.27 -2.5 4,986,777 + Pf1N1B4_4523 0.80 +0.1 4,986,778 - Pf1N1B4_4523 0.80 +0.1 4,986,778 - Pf1N1B4_4523 0.80 -1.3 4,986,956 - +0.1
Or see this region's nucleotide sequence