Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4378 and Pf1N1B4_4379 are separated by 175 nucleotides Pf1N1B4_4379 and Pf1N1B4_4380 are separated by 51 nucleotides
Pf1N1B4_4378 - Kup system potassium uptake protein, at 4,835,129 to 4,837,030
_4378
Pf1N1B4_4379 - Nicotinamidase family protein YcaC, at 4,837,206 to 4,837,790
_4379
Pf1N1B4_4380 - oxidoreductase, at 4,837,842 to 4,838,912
_4380
Position (kb)
4837
4838 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4836.261 kb on - strand, within Pf1N1B4_4378 at 4836.292 kb on + strand, within Pf1N1B4_4378 at 4836.318 kb on + strand, within Pf1N1B4_4378 at 4836.318 kb on + strand, within Pf1N1B4_4378 at 4836.318 kb on + strand, within Pf1N1B4_4378 at 4836.318 kb on + strand, within Pf1N1B4_4378 at 4836.360 kb on + strand, within Pf1N1B4_4378 at 4836.392 kb on + strand, within Pf1N1B4_4378 at 4836.393 kb on - strand, within Pf1N1B4_4378 at 4836.747 kb on - strand, within Pf1N1B4_4378 at 4836.866 kb on + strand at 4836.866 kb on + strand at 4836.867 kb on - strand at 4836.974 kb on + strand at 4836.974 kb on + strand at 4837.028 kb on + strand at 4837.069 kb on + strand at 4837.069 kb on + strand at 4837.069 kb on + strand at 4837.069 kb on + strand at 4837.075 kb on + strand at 4837.208 kb on - strand at 4837.609 kb on + strand, within Pf1N1B4_4379 at 4837.609 kb on + strand, within Pf1N1B4_4379 at 4837.808 kb on + strand at 4837.809 kb on - strand at 4837.809 kb on - strand at 4837.809 kb on - strand at 4837.882 kb on + strand at 4837.882 kb on + strand at 4837.882 kb on + strand at 4837.882 kb on + strand at 4837.883 kb on - strand at 4837.903 kb on + strand at 4837.903 kb on + strand at 4837.904 kb on - strand at 4837.929 kb on - strand at 4837.929 kb on - strand at 4838.065 kb on + strand, within Pf1N1B4_4380 at 4838.137 kb on + strand at 4838.137 kb on + strand at 4838.179 kb on + strand at 4838.179 kb on + strand, within Pf1N1B4_4380 at 4838.179 kb on + strand, within Pf1N1B4_4380 at 4838.180 kb on - strand at 4838.327 kb on - strand, within Pf1N1B4_4380 at 4838.649 kb on + strand, within Pf1N1B4_4380 at 4838.650 kb on - strand at 4838.650 kb on - strand, within Pf1N1B4_4380 at 4838.708 kb on + strand, within Pf1N1B4_4380 at 4838.716 kb on + strand, within Pf1N1B4_4380 at 4838.789 kb on - strand, within Pf1N1B4_4380 at 4838.789 kb on - strand, within Pf1N1B4_4380 at 4838.789 kb on - strand, within Pf1N1B4_4380 at 4838.789 kb on - strand, within Pf1N1B4_4380
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 4,836,261 - Pf1N1B4_4378 0.60 -1.8 4,836,292 + Pf1N1B4_4378 0.61 +0.1 4,836,318 + Pf1N1B4_4378 0.63 +0.1 4,836,318 + Pf1N1B4_4378 0.63 -1.2 4,836,318 + Pf1N1B4_4378 0.63 +0.1 4,836,318 + Pf1N1B4_4378 0.63 -2.6 4,836,360 + Pf1N1B4_4378 0.65 +0.1 4,836,392 + Pf1N1B4_4378 0.66 -2.8 4,836,393 - Pf1N1B4_4378 0.66 -3.1 4,836,747 - Pf1N1B4_4378 0.85 +0.1 4,836,866 + +0.1 4,836,866 + -1.9 4,836,867 - -2.7 4,836,974 + -2.2 4,836,974 + +0.1 4,837,028 + +0.1 4,837,069 + +0.1 4,837,069 + +0.1 4,837,069 + -3.9 4,837,069 + +0.1 4,837,075 + -2.4 4,837,208 - -2.6 4,837,609 + Pf1N1B4_4379 0.69 -0.9 4,837,609 + Pf1N1B4_4379 0.69 -1.0 4,837,808 + -1.5 4,837,809 - -2.4 4,837,809 - +0.1 4,837,809 - +0.1 4,837,882 + -1.5 4,837,882 + -2.2 4,837,882 + -0.9 4,837,882 + +0.1 4,837,883 - +0.1 4,837,903 + +0.1 4,837,903 + -4.0 4,837,904 - +0.1 4,837,929 - -1.6 4,837,929 - -2.1 4,838,065 + Pf1N1B4_4380 0.21 -1.1 4,838,137 + +0.1 4,838,137 + -2.2 4,838,179 + -0.9 4,838,179 + Pf1N1B4_4380 0.31 +0.1 4,838,179 + Pf1N1B4_4380 0.31 +0.1 4,838,180 - -2.3 4,838,327 - Pf1N1B4_4380 0.45 +0.1 4,838,649 + Pf1N1B4_4380 0.75 -0.8 4,838,650 - +0.1 4,838,650 - Pf1N1B4_4380 0.75 -1.9 4,838,708 + Pf1N1B4_4380 0.81 +0.1 4,838,716 + Pf1N1B4_4380 0.82 -1.0 4,838,789 - Pf1N1B4_4380 0.88 +0.1 4,838,789 - Pf1N1B4_4380 0.88 +1.1 4,838,789 - Pf1N1B4_4380 0.88 +0.1 4,838,789 - Pf1N1B4_4380 0.88 +0.1
Or see this region's nucleotide sequence