Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4122 and Pf1N1B4_4123 are separated by 48 nucleotides Pf1N1B4_4123 and Pf1N1B4_4124 are separated by 125 nucleotides Pf1N1B4_4124 and Pf1N1B4_4125 are separated by 490 nucleotides
Pf1N1B4_4122 - Acetyltransferase, at 4,563,562 to 4,564,062
_4122
Pf1N1B4_4123 - Isochorismate pyruvate-lyase (EC 4.-.-.-), at 4,564,111 to 4,564,431
_4123
Pf1N1B4_4124 - Nicotinamidase/isochorismatase family protein, at 4,564,557 to 4,564,889
_4124
Pf1N1B4_4125 - hypothetical protein, at 4,565,380 to 4,565,922
_4125
Position (kb)
4564
4565 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4563.564 kb on - strand at 4563.614 kb on - strand, within Pf1N1B4_4122 at 4563.664 kb on - strand at 4563.675 kb on - strand, within Pf1N1B4_4122 at 4563.675 kb on - strand, within Pf1N1B4_4122 at 4563.676 kb on + strand, within Pf1N1B4_4122 at 4563.676 kb on + strand, within Pf1N1B4_4122 at 4563.676 kb on + strand, within Pf1N1B4_4122 at 4563.677 kb on - strand, within Pf1N1B4_4122 at 4563.677 kb on - strand, within Pf1N1B4_4122 at 4563.677 kb on - strand, within Pf1N1B4_4122 at 4563.847 kb on - strand at 4563.847 kb on - strand, within Pf1N1B4_4122 at 4563.847 kb on - strand, within Pf1N1B4_4122 at 4563.847 kb on - strand, within Pf1N1B4_4122 at 4563.904 kb on + strand, within Pf1N1B4_4122 at 4563.904 kb on + strand, within Pf1N1B4_4122 at 4563.905 kb on - strand at 4563.908 kb on + strand, within Pf1N1B4_4122 at 4563.909 kb on - strand, within Pf1N1B4_4122 at 4563.944 kb on + strand, within Pf1N1B4_4122 at 4563.945 kb on - strand, within Pf1N1B4_4122 at 4564.029 kb on + strand at 4564.029 kb on + strand at 4564.036 kb on + strand at 4564.036 kb on + strand at 4564.036 kb on + strand at 4564.041 kb on + strand at 4564.062 kb on + strand at 4564.062 kb on + strand at 4564.063 kb on - strand at 4564.089 kb on + strand at 4564.089 kb on + strand at 4564.089 kb on + strand at 4564.089 kb on + strand at 4564.090 kb on - strand at 4564.090 kb on - strand at 4564.090 kb on - strand at 4564.181 kb on + strand, within Pf1N1B4_4123 at 4564.181 kb on + strand at 4564.182 kb on - strand, within Pf1N1B4_4123 at 4564.316 kb on + strand, within Pf1N1B4_4123 at 4564.316 kb on + strand, within Pf1N1B4_4123 at 4564.412 kb on - strand at 4564.412 kb on - strand at 4564.433 kb on + strand at 4564.478 kb on + strand at 4564.478 kb on + strand at 4564.479 kb on - strand at 4564.479 kb on - strand at 4564.788 kb on + strand, within Pf1N1B4_4124 at 4564.860 kb on + strand at 4564.861 kb on - strand at 4564.908 kb on - strand at 4564.926 kb on + strand at 4564.926 kb on + strand at 4564.927 kb on - strand at 4564.982 kb on + strand at 4564.983 kb on - strand at 4565.062 kb on - strand at 4565.062 kb on - strand at 4565.062 kb on - strand at 4565.062 kb on - strand at 4565.071 kb on + strand at 4565.071 kb on + strand at 4565.071 kb on + strand at 4565.071 kb on + strand at 4565.071 kb on + strand at 4565.071 kb on + strand at 4565.071 kb on + strand at 4565.071 kb on + strand at 4565.071 kb on + strand at 4565.071 kb on + strand at 4565.071 kb on + strand at 4565.072 kb on - strand at 4565.072 kb on - strand at 4565.072 kb on - strand at 4565.072 kb on - strand at 4565.072 kb on - strand at 4565.072 kb on - strand at 4565.072 kb on - strand at 4565.072 kb on - strand at 4565.072 kb on - strand at 4565.072 kb on - strand at 4565.072 kb on - strand at 4565.072 kb on - strand at 4565.075 kb on + strand at 4565.076 kb on - strand at 4565.076 kb on - strand at 4565.076 kb on - strand at 4565.076 kb on - strand at 4565.076 kb on - strand at 4565.242 kb on - strand at 4565.242 kb on - strand at 4565.242 kb on - strand at 4565.254 kb on - strand at 4565.254 kb on - strand at 4565.254 kb on - strand at 4565.254 kb on - strand at 4565.259 kb on + strand at 4565.312 kb on + strand at 4565.312 kb on + strand at 4565.313 kb on - strand at 4565.313 kb on - strand at 4565.313 kb on - strand at 4565.313 kb on - strand at 4565.313 kb on - strand at 4565.350 kb on - strand at 4565.357 kb on + strand at 4565.358 kb on - strand at 4565.399 kb on + strand at 4565.488 kb on - strand, within Pf1N1B4_4125 at 4565.488 kb on - strand, within Pf1N1B4_4125 at 4565.488 kb on - strand, within Pf1N1B4_4125 at 4565.615 kb on - strand, within Pf1N1B4_4125 at 4565.629 kb on - strand, within Pf1N1B4_4125 at 4565.629 kb on - strand, within Pf1N1B4_4125 at 4565.629 kb on - strand, within Pf1N1B4_4125 at 4565.676 kb on - strand, within Pf1N1B4_4125 at 4565.846 kb on + strand at 4565.846 kb on + strand, within Pf1N1B4_4125 at 4565.847 kb on - strand, within Pf1N1B4_4125 at 4565.847 kb on - strand, within Pf1N1B4_4125 at 4565.847 kb on - strand, within Pf1N1B4_4125 at 4565.847 kb on - strand, within Pf1N1B4_4125
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 4,563,564 - -0.0 4,563,614 - Pf1N1B4_4122 0.10 -3.4 4,563,664 - -1.5 4,563,675 - Pf1N1B4_4122 0.23 -0.0 4,563,675 - Pf1N1B4_4122 0.23 -2.7 4,563,676 + Pf1N1B4_4122 0.23 -1.9 4,563,676 + Pf1N1B4_4122 0.23 -2.6 4,563,676 + Pf1N1B4_4122 0.23 -0.0 4,563,677 - Pf1N1B4_4122 0.23 -1.8 4,563,677 - Pf1N1B4_4122 0.23 -0.0 4,563,677 - Pf1N1B4_4122 0.23 -2.0 4,563,847 - -0.0 4,563,847 - Pf1N1B4_4122 0.57 -0.0 4,563,847 - Pf1N1B4_4122 0.57 -0.9 4,563,847 - Pf1N1B4_4122 0.57 -2.4 4,563,904 + Pf1N1B4_4122 0.68 -2.2 4,563,904 + Pf1N1B4_4122 0.68 -3.0 4,563,905 - -0.0 4,563,908 + Pf1N1B4_4122 0.69 -0.0 4,563,909 - Pf1N1B4_4122 0.69 -0.0 4,563,944 + Pf1N1B4_4122 0.76 -4.4 4,563,945 - Pf1N1B4_4122 0.76 -0.0 4,564,029 + -1.8 4,564,029 + -1.4 4,564,036 + -3.4 4,564,036 + -1.0 4,564,036 + -2.5 4,564,041 + -1.3 4,564,062 + -1.4 4,564,062 + -0.0 4,564,063 - -0.0 4,564,089 + -0.0 4,564,089 + -0.0 4,564,089 + -4.3 4,564,089 + -0.0 4,564,090 - -0.0 4,564,090 - -0.0 4,564,090 - -2.8 4,564,181 + Pf1N1B4_4123 0.22 -0.0 4,564,181 + -0.0 4,564,182 - Pf1N1B4_4123 0.22 -1.5 4,564,316 + Pf1N1B4_4123 0.64 -0.6 4,564,316 + Pf1N1B4_4123 0.64 -2.5 4,564,412 - -0.0 4,564,412 - -0.0 4,564,433 + -2.5 4,564,478 + -0.0 4,564,478 + -0.0 4,564,479 - -3.1 4,564,479 - -0.0 4,564,788 + Pf1N1B4_4124 0.69 -0.9 4,564,860 + -0.0 4,564,861 - -0.0 4,564,908 - -0.0 4,564,926 + -1.9 4,564,926 + -0.0 4,564,927 - -0.0 4,564,982 + -3.0 4,564,983 - -1.7 4,565,062 - -0.0 4,565,062 - -0.0 4,565,062 - -3.4 4,565,062 - -0.9 4,565,071 + -2.7 4,565,071 + -0.0 4,565,071 + -0.0 4,565,071 + -0.0 4,565,071 + -3.6 4,565,071 + -1.9 4,565,071 + -1.1 4,565,071 + -0.0 4,565,071 + -1.8 4,565,071 + -1.9 4,565,071 + -2.0 4,565,072 - -2.0 4,565,072 - -2.1 4,565,072 - -1.5 4,565,072 - -1.8 4,565,072 - -0.0 4,565,072 - -0.0 4,565,072 - -0.4 4,565,072 - -0.0 4,565,072 - -2.4 4,565,072 - -0.0 4,565,072 - -0.3 4,565,072 - -0.0 4,565,075 + -0.0 4,565,076 - -2.1 4,565,076 - -0.0 4,565,076 - -0.0 4,565,076 - -0.0 4,565,076 - -0.0 4,565,242 - -3.0 4,565,242 - -0.0 4,565,242 - -2.6 4,565,254 - -1.6 4,565,254 - -0.0 4,565,254 - -0.0 4,565,254 - -0.0 4,565,259 + -0.0 4,565,312 + -0.0 4,565,312 + -0.0 4,565,313 - -0.0 4,565,313 - -0.0 4,565,313 - -0.0 4,565,313 - -0.0 4,565,313 - -2.3 4,565,350 - -0.0 4,565,357 + -0.0 4,565,358 - -0.0 4,565,399 + -0.0 4,565,488 - Pf1N1B4_4125 0.20 +0.1 4,565,488 - Pf1N1B4_4125 0.20 -0.0 4,565,488 - Pf1N1B4_4125 0.20 -0.0 4,565,615 - Pf1N1B4_4125 0.43 -2.0 4,565,629 - Pf1N1B4_4125 0.46 -0.0 4,565,629 - Pf1N1B4_4125 0.46 -0.0 4,565,629 - Pf1N1B4_4125 0.46 -2.7 4,565,676 - Pf1N1B4_4125 0.55 -0.0 4,565,846 + -0.0 4,565,846 + Pf1N1B4_4125 0.86 -0.0 4,565,847 - Pf1N1B4_4125 0.86 -1.7 4,565,847 - Pf1N1B4_4125 0.86 -0.0 4,565,847 - Pf1N1B4_4125 0.86 -2.8 4,565,847 - Pf1N1B4_4125 0.86 -0.0
Or see this region's nucleotide sequence