Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4119 and Pf1N1B4_4120 are separated by 67 nucleotides Pf1N1B4_4120 and Pf1N1B4_4121 are separated by 101 nucleotides Pf1N1B4_4121 and Pf1N1B4_4122 are separated by 270 nucleotides Pf1N1B4_4122 and Pf1N1B4_4123 are separated by 48 nucleotides
Pf1N1B4_4119 - dioxygenase, at 4,562,080 to 4,562,391
_4119
Pf1N1B4_4120 - hypothetical protein, at 4,562,459 to 4,562,647
_4120
Pf1N1B4_4121 - putative lipoprotein, at 4,562,749 to 4,563,291
_4121
Pf1N1B4_4122 - Acetyltransferase, at 4,563,562 to 4,564,062
_4122
Pf1N1B4_4123 - Isochorismate pyruvate-lyase (EC 4.-.-.-), at 4,564,111 to 4,564,431
_4123
Position (kb)
4562
4563
4564 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4561.837 kb on + strand at 4561.838 kb on - strand at 4561.838 kb on - strand at 4561.838 kb on - strand at 4561.892 kb on + strand at 4561.892 kb on + strand at 4561.892 kb on + strand at 4561.892 kb on + strand at 4561.893 kb on - strand at 4561.893 kb on - strand at 4561.922 kb on + strand at 4561.975 kb on + strand at 4562.079 kb on + strand at 4562.084 kb on + strand at 4562.146 kb on + strand, within Pf1N1B4_4119 at 4562.147 kb on - strand, within Pf1N1B4_4119 at 4562.185 kb on + strand, within Pf1N1B4_4119 at 4562.186 kb on - strand, within Pf1N1B4_4119 at 4562.186 kb on - strand, within Pf1N1B4_4119 at 4562.279 kb on + strand, within Pf1N1B4_4119 at 4562.279 kb on + strand, within Pf1N1B4_4119 at 4562.417 kb on + strand at 4562.587 kb on + strand, within Pf1N1B4_4120 at 4562.588 kb on - strand, within Pf1N1B4_4120 at 4562.646 kb on - strand at 4562.646 kb on - strand at 4562.713 kb on + strand at 4562.714 kb on - strand at 4562.714 kb on - strand at 4562.829 kb on - strand, within Pf1N1B4_4121 at 4562.888 kb on - strand, within Pf1N1B4_4121 at 4562.888 kb on - strand, within Pf1N1B4_4121 at 4562.932 kb on + strand at 4562.932 kb on + strand at 4562.933 kb on - strand, within Pf1N1B4_4121 at 4562.933 kb on - strand at 4562.933 kb on - strand, within Pf1N1B4_4121 at 4562.936 kb on + strand, within Pf1N1B4_4121 at 4562.936 kb on + strand, within Pf1N1B4_4121 at 4562.936 kb on + strand, within Pf1N1B4_4121 at 4562.937 kb on - strand, within Pf1N1B4_4121 at 4562.937 kb on - strand, within Pf1N1B4_4121 at 4562.937 kb on - strand, within Pf1N1B4_4121 at 4562.937 kb on - strand at 4562.937 kb on - strand, within Pf1N1B4_4121 at 4562.937 kb on - strand, within Pf1N1B4_4121 at 4562.973 kb on - strand, within Pf1N1B4_4121 at 4562.979 kb on - strand, within Pf1N1B4_4121 at 4563.026 kb on + strand, within Pf1N1B4_4121 at 4563.027 kb on - strand, within Pf1N1B4_4121 at 4563.059 kb on - strand, within Pf1N1B4_4121 at 4563.059 kb on - strand, within Pf1N1B4_4121 at 4563.072 kb on - strand, within Pf1N1B4_4121 at 4563.170 kb on + strand, within Pf1N1B4_4121 at 4563.306 kb on + strand at 4563.310 kb on - strand at 4563.310 kb on - strand at 4563.398 kb on + strand at 4563.403 kb on + strand at 4563.404 kb on - strand at 4563.413 kb on - strand at 4563.564 kb on - strand at 4563.614 kb on - strand, within Pf1N1B4_4122 at 4563.664 kb on - strand at 4563.675 kb on - strand, within Pf1N1B4_4122 at 4563.675 kb on - strand, within Pf1N1B4_4122 at 4563.676 kb on + strand, within Pf1N1B4_4122 at 4563.676 kb on + strand, within Pf1N1B4_4122 at 4563.676 kb on + strand, within Pf1N1B4_4122 at 4563.677 kb on - strand, within Pf1N1B4_4122 at 4563.677 kb on - strand, within Pf1N1B4_4122 at 4563.677 kb on - strand, within Pf1N1B4_4122 at 4563.847 kb on - strand at 4563.847 kb on - strand, within Pf1N1B4_4122 at 4563.847 kb on - strand, within Pf1N1B4_4122 at 4563.847 kb on - strand, within Pf1N1B4_4122 at 4563.904 kb on + strand, within Pf1N1B4_4122 at 4563.904 kb on + strand, within Pf1N1B4_4122 at 4563.905 kb on - strand at 4563.908 kb on + strand, within Pf1N1B4_4122 at 4563.909 kb on - strand, within Pf1N1B4_4122 at 4563.944 kb on + strand, within Pf1N1B4_4122 at 4563.945 kb on - strand, within Pf1N1B4_4122 at 4564.029 kb on + strand at 4564.029 kb on + strand at 4564.036 kb on + strand at 4564.036 kb on + strand at 4564.036 kb on + strand at 4564.041 kb on + strand at 4564.062 kb on + strand at 4564.062 kb on + strand at 4564.063 kb on - strand at 4564.089 kb on + strand at 4564.089 kb on + strand at 4564.089 kb on + strand at 4564.089 kb on + strand at 4564.090 kb on - strand at 4564.090 kb on - strand at 4564.090 kb on - strand at 4564.181 kb on + strand, within Pf1N1B4_4123 at 4564.181 kb on + strand at 4564.182 kb on - strand, within Pf1N1B4_4123
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 4,561,837 + -0.0 4,561,838 - -1.3 4,561,838 - -3.6 4,561,838 - -0.0 4,561,892 + -0.0 4,561,892 + -0.8 4,561,892 + -0.0 4,561,892 + -0.0 4,561,893 - -1.8 4,561,893 - -0.0 4,561,922 + -2.2 4,561,975 + -0.0 4,562,079 + -0.0 4,562,084 + -0.0 4,562,146 + Pf1N1B4_4119 0.21 -0.0 4,562,147 - Pf1N1B4_4119 0.21 -3.5 4,562,185 + Pf1N1B4_4119 0.34 -0.9 4,562,186 - Pf1N1B4_4119 0.34 -0.0 4,562,186 - Pf1N1B4_4119 0.34 -2.2 4,562,279 + Pf1N1B4_4119 0.64 -0.0 4,562,279 + Pf1N1B4_4119 0.64 -0.0 4,562,417 + -0.0 4,562,587 + Pf1N1B4_4120 0.68 -0.0 4,562,588 - Pf1N1B4_4120 0.68 -1.6 4,562,646 - -2.9 4,562,646 - -0.0 4,562,713 + -3.5 4,562,714 - -0.0 4,562,714 - -0.0 4,562,829 - Pf1N1B4_4121 0.15 -1.0 4,562,888 - Pf1N1B4_4121 0.26 -0.0 4,562,888 - Pf1N1B4_4121 0.26 -3.1 4,562,932 + -1.9 4,562,932 + -2.2 4,562,933 - Pf1N1B4_4121 0.34 -0.0 4,562,933 - -1.4 4,562,933 - Pf1N1B4_4121 0.34 -0.4 4,562,936 + Pf1N1B4_4121 0.34 -0.0 4,562,936 + Pf1N1B4_4121 0.34 -2.2 4,562,936 + Pf1N1B4_4121 0.34 -0.0 4,562,937 - Pf1N1B4_4121 0.35 -1.4 4,562,937 - Pf1N1B4_4121 0.35 -2.4 4,562,937 - Pf1N1B4_4121 0.35 -0.0 4,562,937 - -0.0 4,562,937 - Pf1N1B4_4121 0.35 -0.0 4,562,937 - Pf1N1B4_4121 0.35 -1.0 4,562,973 - Pf1N1B4_4121 0.41 -0.3 4,562,979 - Pf1N1B4_4121 0.42 -2.0 4,563,026 + Pf1N1B4_4121 0.51 -1.8 4,563,027 - Pf1N1B4_4121 0.51 -0.0 4,563,059 - Pf1N1B4_4121 0.57 -0.0 4,563,059 - Pf1N1B4_4121 0.57 -0.0 4,563,072 - Pf1N1B4_4121 0.59 -0.0 4,563,170 + Pf1N1B4_4121 0.78 -0.0 4,563,306 + -1.4 4,563,310 - -1.5 4,563,310 - -3.0 4,563,398 + -0.0 4,563,403 + -0.0 4,563,404 - -2.3 4,563,413 - -1.7 4,563,564 - -0.0 4,563,614 - Pf1N1B4_4122 0.10 -3.4 4,563,664 - -1.5 4,563,675 - Pf1N1B4_4122 0.23 -0.0 4,563,675 - Pf1N1B4_4122 0.23 -2.7 4,563,676 + Pf1N1B4_4122 0.23 -1.9 4,563,676 + Pf1N1B4_4122 0.23 -2.6 4,563,676 + Pf1N1B4_4122 0.23 -0.0 4,563,677 - Pf1N1B4_4122 0.23 -1.8 4,563,677 - Pf1N1B4_4122 0.23 -0.0 4,563,677 - Pf1N1B4_4122 0.23 -2.0 4,563,847 - -0.0 4,563,847 - Pf1N1B4_4122 0.57 -0.0 4,563,847 - Pf1N1B4_4122 0.57 -0.9 4,563,847 - Pf1N1B4_4122 0.57 -2.4 4,563,904 + Pf1N1B4_4122 0.68 -2.2 4,563,904 + Pf1N1B4_4122 0.68 -3.0 4,563,905 - -0.0 4,563,908 + Pf1N1B4_4122 0.69 -0.0 4,563,909 - Pf1N1B4_4122 0.69 -0.0 4,563,944 + Pf1N1B4_4122 0.76 -4.4 4,563,945 - Pf1N1B4_4122 0.76 -0.0 4,564,029 + -1.8 4,564,029 + -1.4 4,564,036 + -3.4 4,564,036 + -1.0 4,564,036 + -2.5 4,564,041 + -1.3 4,564,062 + -1.4 4,564,062 + -0.0 4,564,063 - -0.0 4,564,089 + -0.0 4,564,089 + -0.0 4,564,089 + -4.3 4,564,089 + -0.0 4,564,090 - -0.0 4,564,090 - -0.0 4,564,090 - -2.8 4,564,181 + Pf1N1B4_4123 0.22 -0.0 4,564,181 + -0.0 4,564,182 - Pf1N1B4_4123 0.22 -1.5
Or see this region's nucleotide sequence