Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4064 and Pf1N1B4_4065 are separated by 0 nucleotides Pf1N1B4_4065 and Pf1N1B4_4066 are separated by 139 nucleotides
Pf1N1B4_4064 - Seryl-tRNA synthetase (EC 6.1.1.11), at 4,518,009 to 4,519,289
_4064
Pf1N1B4_4065 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 4,519,290 to 4,520,684
_4065
Pf1N1B4_4066 - Glutathione S-transferase, omega (EC 2.5.1.18), at 4,520,824 to 4,521,828
_4066
Position (kb)
4519
4520
4521 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4519.287 kb on + strand at 4519.296 kb on + strand at 4519.296 kb on + strand at 4519.296 kb on + strand at 4519.297 kb on - strand at 4519.333 kb on + strand at 4519.333 kb on + strand at 4519.408 kb on + strand at 4519.408 kb on + strand at 4519.741 kb on - strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.029 kb on - strand, within Pf1N1B4_4065 at 4520.029 kb on - strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.204 kb on + strand, within Pf1N1B4_4065 at 4520.205 kb on - strand, within Pf1N1B4_4065 at 4520.304 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.434 kb on - strand, within Pf1N1B4_4065 at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.597 kb on - strand at 4520.597 kb on - strand at 4520.597 kb on - strand at 4520.727 kb on - strand at 4520.949 kb on - strand, within Pf1N1B4_4066 at 4520.949 kb on - strand, within Pf1N1B4_4066 at 4520.951 kb on + strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4521.006 kb on - strand, within Pf1N1B4_4066 at 4521.006 kb on - strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.081 kb on - strand, within Pf1N1B4_4066 at 4521.200 kb on - strand at 4521.221 kb on + strand, within Pf1N1B4_4066 at 4521.222 kb on - strand, within Pf1N1B4_4066 at 4521.222 kb on - strand, within Pf1N1B4_4066 at 4521.479 kb on + strand, within Pf1N1B4_4066 at 4521.662 kb on + strand at 4521.663 kb on - strand, within Pf1N1B4_4066
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 4,519,287 + -3.2 4,519,296 + -2.9 4,519,296 + -0.0 4,519,296 + -0.0 4,519,297 - -2.6 4,519,333 + -1.0 4,519,333 + -0.0 4,519,408 + -0.0 4,519,408 + -0.0 4,519,741 - Pf1N1B4_4065 0.32 -0.0 4,519,834 + Pf1N1B4_4065 0.39 -0.0 4,519,834 + Pf1N1B4_4065 0.39 -0.0 4,519,834 + -5.6 4,519,834 + Pf1N1B4_4065 0.39 -0.0 4,519,834 + Pf1N1B4_4065 0.39 -0.0 4,519,834 + Pf1N1B4_4065 0.39 -0.0 4,519,834 + Pf1N1B4_4065 0.39 -3.8 4,519,835 - Pf1N1B4_4065 0.39 -4.7 4,519,835 - Pf1N1B4_4065 0.39 -2.3 4,519,835 - Pf1N1B4_4065 0.39 -0.0 4,519,835 - Pf1N1B4_4065 0.39 -0.0 4,519,835 - Pf1N1B4_4065 0.39 -0.0 4,520,028 + Pf1N1B4_4065 0.53 -0.0 4,520,028 + Pf1N1B4_4065 0.53 -2.8 4,520,028 + Pf1N1B4_4065 0.53 -0.0 4,520,028 + Pf1N1B4_4065 0.53 -0.0 4,520,028 + Pf1N1B4_4065 0.53 -4.8 4,520,028 + Pf1N1B4_4065 0.53 -0.0 4,520,029 - Pf1N1B4_4065 0.53 -3.6 4,520,029 - Pf1N1B4_4065 0.53 -0.0 4,520,088 + Pf1N1B4_4065 0.57 -4.8 4,520,088 + Pf1N1B4_4065 0.57 -0.0 4,520,088 + Pf1N1B4_4065 0.57 -5.6 4,520,088 + Pf1N1B4_4065 0.57 -0.0 4,520,088 + Pf1N1B4_4065 0.57 -3.7 4,520,204 + Pf1N1B4_4065 0.66 -5.0 4,520,205 - Pf1N1B4_4065 0.66 -0.0 4,520,304 + Pf1N1B4_4065 0.73 -0.0 4,520,433 + Pf1N1B4_4065 0.82 -0.0 4,520,433 + Pf1N1B4_4065 0.82 -0.0 4,520,433 + Pf1N1B4_4065 0.82 -4.1 4,520,433 + Pf1N1B4_4065 0.82 -0.0 4,520,433 + Pf1N1B4_4065 0.82 -0.0 4,520,433 + Pf1N1B4_4065 0.82 -0.0 4,520,434 - Pf1N1B4_4065 0.82 -0.0 4,520,596 + -0.0 4,520,596 + -3.7 4,520,596 + -2.1 4,520,596 + -2.9 4,520,596 + -0.0 4,520,596 + -2.5 4,520,596 + -0.0 4,520,596 + -0.0 4,520,597 - -4.1 4,520,597 - -0.0 4,520,597 - -3.9 4,520,727 - -0.0 4,520,949 - Pf1N1B4_4066 0.12 -2.6 4,520,949 - Pf1N1B4_4066 0.12 -0.0 4,520,951 + Pf1N1B4_4066 0.13 -3.2 4,520,952 - Pf1N1B4_4066 0.13 -0.0 4,520,952 - Pf1N1B4_4066 0.13 -0.0 4,520,952 - Pf1N1B4_4066 0.13 -0.0 4,520,952 - Pf1N1B4_4066 0.13 -0.0 4,521,006 - Pf1N1B4_4066 0.18 -0.0 4,521,006 - Pf1N1B4_4066 0.18 -1.8 4,521,080 + Pf1N1B4_4066 0.25 -1.7 4,521,080 + Pf1N1B4_4066 0.25 -1.9 4,521,080 + Pf1N1B4_4066 0.25 -4.6 4,521,081 - Pf1N1B4_4066 0.26 -1.9 4,521,200 - -0.0 4,521,221 + Pf1N1B4_4066 0.40 -1.7 4,521,222 - Pf1N1B4_4066 0.40 -0.0 4,521,222 - Pf1N1B4_4066 0.40 -0.0 4,521,479 + Pf1N1B4_4066 0.65 -0.0 4,521,662 + -1.1 4,521,663 - Pf1N1B4_4066 0.83 -3.2
Or see this region's nucleotide sequence