Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3876

Experiment: Nitrate reduction, replicate A t2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction, replicate A t2
4,305,419	+				-2.4
4,305,456	+		Pf1N1B4_3875	0.14	-0.0
4,305,520	+		Pf1N1B4_3875	0.20	-2.0
4,305,567	+		Pf1N1B4_3875	0.25	-0.0
4,305,591	+		Pf1N1B4_3875	0.27	-0.0
4,305,592	-		Pf1N1B4_3875	0.27	-0.0
4,305,625	+		Pf1N1B4_3875	0.30	-1.0
4,305,626	-		Pf1N1B4_3875	0.30	-2.6
4,305,664	+		Pf1N1B4_3875	0.34	-0.0
4,305,665	-		Pf1N1B4_3875	0.34	-4.5
4,305,665	-		Pf1N1B4_3875	0.34	-2.6
4,305,670	+		Pf1N1B4_3875	0.35	-0.0
4,305,670	+				-0.0
4,305,670	+				-0.0
4,305,671	-		Pf1N1B4_3875	0.35	-0.0
4,305,705	-		Pf1N1B4_3875	0.38	-1.6
4,305,705	-		Pf1N1B4_3875	0.38	-0.7
4,305,742	-		Pf1N1B4_3875	0.42	-0.4
4,306,071	+		Pf1N1B4_3875	0.75	-0.0
4,306,090	+		Pf1N1B4_3875	0.76	-1.5
4,306,090	+		Pf1N1B4_3875	0.76	-0.0
4,306,090	+		Pf1N1B4_3875	0.76	-0.0
4,306,106	+		Pf1N1B4_3875	0.78	-0.0
4,306,137	+		Pf1N1B4_3875	0.81	-2.2
4,306,137	+		Pf1N1B4_3875	0.81	-0.0
4,306,137	+		Pf1N1B4_3875	0.81	-1.4
4,306,137	+		Pf1N1B4_3875	0.81	-0.3
4,306,137	+		Pf1N1B4_3875	0.81	-2.1
4,306,137	+		Pf1N1B4_3875	0.81	-0.0
4,306,138	-		Pf1N1B4_3875	0.81	-0.0
4,306,143	+		Pf1N1B4_3875	0.82	-0.0
4,306,143	+		Pf1N1B4_3875	0.82	-0.0
4,306,143	+		Pf1N1B4_3875	0.82	-0.0
4,306,143	+		Pf1N1B4_3875	0.82	-0.0
4,306,143	+		Pf1N1B4_3875	0.82	-0.8
4,306,143	+		Pf1N1B4_3875	0.82	-2.4
4,306,143	+		Pf1N1B4_3875	0.82	-2.1
4,306,143	+		Pf1N1B4_3875	0.82	-0.0
4,306,143	+		Pf1N1B4_3875	0.82	-0.0
4,306,143	+		Pf1N1B4_3875	0.82	-0.0
4,306,143	+				-2.7
4,306,143	+		Pf1N1B4_3875	0.82	-0.0
4,306,143	+		Pf1N1B4_3875	0.82	-0.0
4,306,143	+		Pf1N1B4_3875	0.82	-1.5
4,306,143	+		Pf1N1B4_3875	0.82	-2.2
4,306,143	+				-0.0
4,306,143	+		Pf1N1B4_3875	0.82	-0.0
4,306,143	+				-2.3
4,306,143	+		Pf1N1B4_3875	0.82	-0.0
4,306,144	-		Pf1N1B4_3875	0.82	-1.3
4,306,144	-				-0.0
4,306,144	-		Pf1N1B4_3875	0.82	-0.0
4,306,144	-				-0.0
4,306,147	+		Pf1N1B4_3875	0.82	-2.5
4,306,147	+		Pf1N1B4_3875	0.82	-0.0
4,306,147	+		Pf1N1B4_3875	0.82	-1.8
4,306,147	+				-0.0
4,306,147	+		Pf1N1B4_3875	0.82	-0.0
4,306,216	+		Pf1N1B4_3875	0.89	-1.5
4,306,221	+		Pf1N1B4_3875	0.89	-1.7
4,306,221	+		Pf1N1B4_3875	0.89	-2.0
4,306,221	+		Pf1N1B4_3875	0.89	-0.0
4,306,268	+				-2.4
4,306,278	+				-0.0
4,306,278	+				-0.0
4,306,278	+				-0.0
4,306,278	+				-0.0
4,306,324	+				-0.0
4,306,324	+				-0.6
4,306,324	+				-0.0
4,306,324	+				-3.7
4,306,324	+				-0.0
4,306,325	-				-0.0
4,306,453	+				-1.6
4,306,454	-				-0.0
4,306,454	-				-0.8
4,306,454	-				-3.3
4,306,454	-				-0.0
4,306,459	+				-0.5
4,306,459	+				-0.0
4,306,459	+				-0.0
4,306,459	+				-4.8
4,306,459	+				-0.0
4,306,459	+				-1.3
4,306,459	+				-0.7
4,306,459	+				-4.0
4,306,460	-				-2.2
4,306,460	-				-1.2
4,306,460	-				-2.4
4,306,460	-				-0.0
4,306,570	+		Pf1N1B4_3876	0.17	-0.0
4,306,570	+		Pf1N1B4_3876	0.17	-2.9
4,306,570	+		Pf1N1B4_3876	0.17	-0.0
4,306,570	+		Pf1N1B4_3876	0.17	-1.4
4,306,570	+		Pf1N1B4_3876	0.17	-0.0
4,306,570	+		Pf1N1B4_3876	0.17	-0.0
4,306,571	-		Pf1N1B4_3876	0.17	-1.5
4,306,571	-		Pf1N1B4_3876	0.17	-2.6
4,306,571	-		Pf1N1B4_3876	0.17	-2.7
4,306,571	-		Pf1N1B4_3876	0.17	-1.6
4,306,571	-		Pf1N1B4_3876	0.17	-1.7
4,306,619	+				-0.0
4,306,619	+		Pf1N1B4_3876	0.22	-1.0
4,306,620	-		Pf1N1B4_3876	0.22	-0.0
4,306,739	-		Pf1N1B4_3876	0.34	-0.0
4,306,739	-		Pf1N1B4_3876	0.34	-0.0
4,306,739	-		Pf1N1B4_3876	0.34	-1.8
4,306,739	-		Pf1N1B4_3876	0.34	-2.3
4,306,886	-		Pf1N1B4_3876	0.49	-0.0
4,307,119	+		Pf1N1B4_3876	0.72	-0.0
4,307,120	-		Pf1N1B4_3876	0.72	-0.0
4,307,120	-		Pf1N1B4_3876	0.72	-0.0
4,307,120	-		Pf1N1B4_3876	0.72	-0.0
4,307,120	-		Pf1N1B4_3876	0.72	-1.3
4,307,120	-		Pf1N1B4_3876	0.72	-1.7
4,307,126	-		Pf1N1B4_3876	0.73	-2.7
4,307,126	-		Pf1N1B4_3876	0.73	-2.2
4,307,177	-		Pf1N1B4_3876	0.78	-0.0
4,307,242	+		Pf1N1B4_3876	0.85	-2.0
4,307,243	-		Pf1N1B4_3876	0.85	-0.0
4,307,243	-		Pf1N1B4_3876	0.85	-0.0
4,307,243	-		Pf1N1B4_3876	0.85	-0.0
4,307,243	-		Pf1N1B4_3876	0.85	-1.3
4,307,368	+				-1.3
4,307,368	+				-1.4
4,307,368	+				-0.0
4,307,369	-				-2.2
4,307,369	-				-1.9
4,307,369	-				-0.0
4,307,369	-				-0.0
4,307,369	-				-0.0
4,307,369	-				-1.9
4,307,384	+				-0.5
4,307,384	+				-1.8
4,307,385	-				-2.0
4,307,385	-				-1.7
4,307,385	-				-2.8
4,307,385	-				-0.0
4,307,385	-				-0.0
4,307,385	-				-2.0
4,307,385	-				-0.0
4,307,399	-				-0.0
4,307,404	+				-0.0
4,307,404	+				-0.0
4,307,404	+				-0.0
4,307,405	-				-0.0
4,307,405	-				-0.9
4,307,405	-				-2.2
4,307,574	+				-0.0
4,307,574	+				-0.0
4,307,575	-				-1.0
4,307,575	-				-0.0
4,307,575	-				-0.0
4,307,575	-				-3.0
4,307,575	-				-0.0
4,307,619	-				-0.0
4,307,619	-				-2.2
4,307,690	+		Pf1N1B4_3878	0.18	-0.3
4,307,691	-		Pf1N1B4_3878	0.19	-1.4
4,307,691	-		Pf1N1B4_3878	0.19	-0.0
4,307,691	-		Pf1N1B4_3878	0.19	-3.9
4,307,691	-		Pf1N1B4_3878	0.19	-1.5
4,307,797	+		Pf1N1B4_3878	0.36	-0.0
4,307,797	+		Pf1N1B4_3878	0.36	-0.0
4,307,797	+		Pf1N1B4_3878	0.36	-0.0
4,307,797	+		Pf1N1B4_3878	0.36	-0.0
4,307,798	-		Pf1N1B4_3878	0.36	-1.5
4,307,798	-		Pf1N1B4_3878	0.36	-1.3
4,307,798	-		Pf1N1B4_3878	0.36	-2.6
4,307,798	-		Pf1N1B4_3878	0.36	-0.0
4,307,813	+		Pf1N1B4_3878	0.39	-0.9
4,307,813	+		Pf1N1B4_3878	0.39	-0.0
4,307,814	-		Pf1N1B4_3878	0.39	-0.0
4,307,814	-		Pf1N1B4_3878	0.39	-0.0
4,307,814	-		Pf1N1B4_3878	0.39	-1.3
4,307,814	-		Pf1N1B4_3878	0.39	-0.0
4,307,814	-				-0.0
4,307,814	-		Pf1N1B4_3878	0.39	-3.7
4,307,814	-		Pf1N1B4_3878	0.39	-0.5
4,307,934	-		Pf1N1B4_3878	0.58	-0.0
4,308,011	+		Pf1N1B4_3878	0.71	-0.1
4,308,011	+		Pf1N1B4_3878	0.71	-3.2
4,308,012	-		Pf1N1B4_3878	0.71	-0.0
4,308,012	-		Pf1N1B4_3878	0.71	-1.2
4,308,012	-		Pf1N1B4_3878	0.71	-0.0
4,308,012	-		Pf1N1B4_3878	0.71	-0.0
4,308,159	+				+1.0
4,308,159	+				-0.0
4,308,160	-				-0.7
4,308,201	+				+1.2
4,308,201	+				-0.0
4,308,201	+				-0.0
4,308,202	-				-0.0
4,308,202	-				-2.2
4,308,202	-				-1.4
4,308,202	-				-0.0
4,308,202	-				-2.5
4,308,249	+				-0.0
4,308,249	+				-0.0
4,308,249	+				-0.0
4,308,250	-				-1.5
4,308,250	-				-0.9
4,308,250	-				-0.0
4,308,255	-				-0.0
4,308,261	+				-0.0
4,308,262	-				-0.0
4,308,262	-				-0.0
4,308,268	+				-0.0
4,308,269	-				-0.0
4,308,269	-				-2.0
4,308,269	-				-0.0
4,308,364	+		Pf1N1B4_3879	0.13	-1.7
4,308,365	-		Pf1N1B4_3879	0.13	-0.0
4,308,365	-		Pf1N1B4_3879	0.13	-0.4
4,308,365	-		Pf1N1B4_3879	0.13	-0.0
4,308,365	-		Pf1N1B4_3879	0.13	-3.0